OBJECTIVE:To discuss the status quo and the development strategy of the study of the processing of Chinese herbal medicine.METHODS:The physical-chemical property,the technology transfer and the qualitation and quantitation reported in the literature about the processing of Chinese herbal medicine were analyzed and summarized.The status quo and the current methods for the study of processing of Chinese herbal medicine were expounded and its development strategy was put forward in view of the role and importance of the processing study.RESULTS & CONCLUSION:To develop the study on the processing of Chinese herbal medicine,it is essential to strengthen literature research and summarizing of experiences as well as the combination of processing synergy with clinic practice,attach great importance to the guiding effect of traditional Chinese medicine theory on the study of physical-chemical study in the processing of Chinese herbal medicine,and strengthen study on the key issues including the processing technology of Chinese herbal medicine and the quality control of decoction pieces.

Objective Extracting high content of DHA, EPA from rude fish oil. Methods Extracting fish oil ethyl ester with silver nitrate-water method. Results With silver nitrate-water method extracting three kinds of products from rude fish oil were gained(1) The total content of EPA and DHA mixture was higher than 95%. (2) The content of DHA was higher than 95%. (3) The content of EPA was higher than DHA. Conclusion The researching technology may reduce the production cost of high content of EPA and DHA. The method researched can increase the stabilities of DHA, EPA, and decrease the side effects of the products.

Objective:To evaluate the curative effect of mirror therapy combined with rehabilitation training in the treatment of stroke hemiplegic upper limb motor dysfunction. Methods:To choose 48 patients, were randomly divided into control group, 24 cases in each group to observe, are basic rehabilitation training, observation group joint mirror therapy, using FMA, ARAT, BMI, respectively, evaluation of patients with general motor function, upper limb motor function, daily life ability, numerical measurement of radial carpal extensor emg. Results:After the treatment, the control group, the observation group the FMA level, ARAT level, MBI level, EMG level was higher than that before treatment, the observation group than that in the control group, the observation group FMA, ARAT, MBI, the difference of EMG signals is higher than that of control group, with significant difference(t=14.14,t=4.55,t=5.84, t=9.14;P<0.05).Conclusion: mirror therapy combined with rehabilitation training is really helpful to upper extremity motor functional recovery of stroke patients with hemiplegia, is of positive significance to improve the living ability of the patients.

AIM:To develope a reversed-phase high performance liquid chromatographic method for determing chlorogenic acid and caffeic acid in Fanhuncao Granule(Senecio cannabifolius Less). METHODS: The operation was carried out on Eclipse XDB-C_(18) column with the mobile phase consisting of a mixture of acetonitrile-0.4% phosphoric acid(12(∶)88,V/V).The flow rate of 1.0 mL/min and UV detection wavelength at 327 nm were set to determine the contents of chlorogenic acid and caffieic acid. RESULTS: There was good linear relationship between the concentrations and the peak-areas of chlorogenic acid and caffeic acid.The two kinds of standard solutions were both stable in 16 h(RSD=1.55% for chlorogenic acid,1.11% for caffeic acid) The average recovery was 100.1% and 99.8% for chlorogenic acid and caffeic acid,repectively. CONCLUSION: The method is simple,sensitive,rapid and accurate,and can be used for the quality control of Fanhuncao Granule.

Double template molecularly imprinted polymer ( D_MIP) was prepared by emulsion polymerization method using the nonylphenol (NP), bisphenol A (BPA) as templates, α_methyl methacrylate(MAA) as functional monomer. Then using the D_MIP as solid phase extraction materials, a method based on double_molecularly imprinted solid phase extraction coupled with high performance liquid chromatographic fluorescence detection was developed for the determination of trace NP and BPA from environmental and food samples. The D_MIP was characterized by Fourier transform infrared spectroscopy ( FT_IR ) and adsorption experiments. The results showed that the saturated adsorption capacity of the D_MIP for NP and BPA was 73. 3 and 97. 5 mg/g, respectively, and the relative selectivity coefficient was 2. 2 and 1. 7, respectively. Moreover, the conditions affecting extraction efficiency, such as washing conditions and desorption conditions were optimized. Under the optimal conditions, there was a good linear correlation in the concentration range of 0. 01-2. 3 mg/L with correlation coefficients (R2) greater than 0. 998 for NP and BPA. The detection limits ( S/N=3) were 0. 001 and 0. 002 mg/L. The proposed method was applied to determine NP and BPA in river water, beer and crucian carp samples. The recoveries of NP and BPA were in the range of 86. 4%-99. 1%with the relative standard deviation less than 6 . 2%. This method has good selectivity and high sensitivity and shows good prospects for the application in the enrichment and separation of NP and BPA in real samples.

Objective To discuss the surgical treatment for huge hepatoblastoma in children,and the technique of hepatectomy without blockade of the blood supply to the remained liver lobes.Methods We reviewed 12 cases of huge hepatoblastorna who had been operated from July 2001 to January 2009 in our hospital.The mean age of the children was 3.2 years(range,11 months to 12 years).The diameter of the tumor was from 10 to 23 cm.3～7 cycles of chemotherapy was routinely administrated before operation.When the tumor reduced to a certain size that radical resection could be performed safely,regular hepatectomy was conducted.Hepatoblastoma resection without blocking the blood supply to the remained liver lobes was performed in every patient.Results The operations were successfully accomplished in all the 12 children.5 cases received right trihepatectomy (segment Ⅳ,Ⅴ～Ⅷ),4 cases received right hemihepatectomy(segment Ⅴ～Ⅷ),and the other 3 cases received Ⅳ,Ⅶ,Ⅷ segmentectomy,right Ⅴ,Ⅵ segmentectomy,and left hemihepatectomy respectively.The intraoperative hemodynamic parameters were stable,and there was no perioperative mortality.Postoperative chemotherapy wag routinely administrated.The follow-up period varied from 2 to 92 months.11 children survived and were disease free,among those 6 children have survived for more than 5 years.One child had brain and lung metastasis 5 months post operation,and died 7 months post operation. Conclusion Preoperative chemotherapy administrated to children with huge hepatoblastoma can reduce the tumor size and render tumor reseetable.Hepatoblastoma resection without blocking the blood supply to the remained liver lobes is a safe and feasible surgical technique.

Objective To investigate the status of the plague natural focus and assess the risk of plague in regions with large scale engineering construction project,in order to provide a basis for plague prevention and control.Methods The survey was carried out in 14 regions with large scale engineering construction project of 40 counties in Guizhou Province in 2000-2011,2-3 towns were selected in each county and 2-3 villages in each town,the plague hosts and flea vectors in the residential houses and outdoors were investigated,the sera of 5 animals were collected in each village.The capture rate of hosts and flea index were calculated.Then the samples were cultured and serological tested for plague.Results The density of rodents was 1.14％-3.87％ in the reservoir region' residential building of hydro power station (Tianshengqiao,Pingban,Silin,Guangzhao,Sanbanxi,Dongqing,Qianzhong,Shatuo) and Xingyi airport,and Rattus flavipestus was the dominant species.The density of rodents was 0.00％-0.84％ in the reservoir region of hydro power station (Hongjiadu,Yingzidu,Goupitan,Longtan) and Jinyang district; the total flea index in Yinzidu,Pingban,Longtan reservoir regions of hydro power stations were 3.16,2.00,1.06,3.47 and 1.99,respectively.A total of 21 rodent and flea samples from Tianshengqiao hydro power station were tested positive for F1 antigen of the plague.Three strains of Yersiniapestis were isolated.Nine rat filter paper blood samples and 7 sera of indicative animals were tested plague F1 antibody positive.Other regions with large scale engineering construction project were tested negative.Conclusions The reservoir region of Tianshengqiao hydro power station is endemic areas of the plague,where belong to high risk areas.The density of Rattus flavipestus is higher than the controlling standard of the country in the reservoir region of hydro power station (Pingban,Silin,Guangzhao,Sanbanxi,Dongqing,Qianzhong,Shatuo),where belong to potential risk areas.The plague prevention and monitoring should be continually strengthened to reduce the risk of plague.

This study was aimed to observe clinical effect of Wu-Fu Ma-Xin Gui-Jiang (WFMXGJ) decoction treatment for patients with nonspecific low back pain. A total of 60 nonspecific low back pain cases were selected and randomly divided into the treatment group and the control group, with 30 cases in each group. Patients in the treatment group were treated with modified WFMXGJ decoction, while cases in the control group were given rou-tine treatment of oral administration of nimesulide sustained-release tablets. Seven days were one treatment course. Comparison was made on Visual Analogue Scale/Score (VAS), clinical symptoms and body signs before and after treatment between two groups. The results showed that compared with pretreatment, low back pain were relieved with obviously improved VAS among patients in both groups (P<0.05). However, there was no significant difference between the treatment group and the control group. In the treatment group, acute low back pain VAS was obviously improved than the chronic low back pain VAS (P<0.05). There was no statistical significance between the acute and chronic low back pain in the control group. In the treatment group, the total effective rate was 93.33%;and the con-trol group was 73.33%. The clinical effect of treatment group was more significant than the control group (P<0.05). There was no statistical significance on acute and chronic low back pain in the treatment group and the control group. The recurrent rate of the treatment group was significantly lower than the control group (P<0.05). In the treatment group, the recurrence rate of chronic low back pain patients was higher (P<0.05). There was no significant difference on the comparison between acute and chronic low back pain in the control group. It was concluded that modified WFMXGJ decoction had obvious therapeutic effect on treatment of nonspecific low back pain, especially for the improvement of acute low back pain, with non-toxic side effects and low recurrence rate. It is worthy of fur-ther research and promotion.

Objective To investigate inhibitory effect of Telmisartan on carotid arterial intima-media thickness in patients with essential hypertension. Methods 104 patients with mild or mid essential hypertension were randomly divided into three groups, which include telmisartan group( telmisartan 80 mg/d PO), ramipril group( ramipril 5 mg/d PO), and control group( other anti-hypertension agents). Blood pressure was monitored during treatment. Carotid arterial intima-media thickness(CIMT) were measured in all patients at beginning and patients who had the 12 months course. Results There were 91 patients who had the 12 months course,which include telmisartan group33 cases,ramipril group28 cases, and control 30 cases. The CIMT was significantly decreased in telmisartan group and ramipril group ( all P < 0. 05 ), and not changed in control while blood pressure fall effectively. In ramipril, group5 cases were ceased the course because of severe cough. Conclusion Both telmisartan and ramipril could decrease blood pressure and CIMT effectively, and there were less side effects in telmisartan group.

Objective To evaluate the in vitro differentiation of human amniotic epithelial cells (hAECs ) into hepatocyte-like cells. Methods Combined approach of dexamethasone, HGF, IGF and other cytokines were used to induce the differentiation of hAECs into hepatocyte-like cells. The induction lasted 2 weeks. During the induction, the expression of albumin ALB, CYP1A1, CYP1A2, IGFR, c-met and key functional genes related to liver cells as well as transcription factors HNF3, HNF4 and C/EBPa were monitored by RT-PCR. Time dependent changes of the surface marker colony ALB, AFP and CK18 were analyzed by cell flow cytometry. Results After the 2 week induction, the expressions of liver hepatocyte-like cell functional genes such as albumin, CYP1A1, CYP1A2, c-met, and transcription factors such as HNF3, HNF4, C/EBPa and HNF1 were observed. Six days after the induction, hAECs mainly were stained AFP+, and the positive rate was (15.1±2.1)%. While 10 days after the induction, part of the hAECs showed AFP+/ALB+ (6.5±1.4)%; and on 14th day, hAECs only showed ALB+, and the rate was (13.9±2.3)%. ALB+ cell increase indicated a gradual functional maturation from the hAECs to hepatocyte-like cells. Similaritly, the number of CK18+ cells in the whole population was also increased: On 10th day, the rate was (16.1±1.2)%; on 14th day, that was (21.3±4.6)%, which proved the above hypothesis of the trandifferentiation. By extending the induction time, the expression of functional genes increased gradually, and a maturing process of hAECs was detected by cell surface markers. Conclusion The differentiation of hAECs induced in vitro has the characteristics of hepatocyte-like cells.