Objective To explore the effects of Acer truncatum oil on oxidative stress and inflammatory reaction in rats after exhaustive exercise to provide a scientific basis for the prevention of sports injury by Acer truncatum oil.Methods Thirty healthy male SD rats were randomly divided into control group (A,normal diet + saline every day),exhaustive group (B,normal diet + saline daily) and exhaustive with Acer truncatum oil group (C,0.21 mL/kg daily Acer truncatum oil gavage),6 weeks later,the rats performed once exhaustive swimming exercise,then the creatine kinase (CK),lactate dehydrogenase (LDH),cytokine interleukin (IL-1β,IL-10) and tumor necrosis factor alpha (TNF-α) in the serum;malondialdehyde (MDA) homogenate reduction glutathione (GSH),superoxide dismutase (SOD),catalase (CAT) and glutathione peroxidase (GSH-Px) in muscle were measured.Results After exhaustive exercise,when compared with the group A,the serum levels of CK,LDH,IL-1,TNF-α and IL-10 were significantly higher than in the group B(P＜0.05,P＜0.01,P＜0.05,P＜0.01,P＜0.05),IL-10/ TNF-α ratio had no statistically significant difference(P＞0.05),the level of MDA in gastrocnemius muscle increased significantly (P＜0.05),GSH level and GSH-PX activity decreased significantly (P＜0.05,P＜0.01),SOD and CAT activity were not statistically significant (P＞0.05).when compared with the group B,serum CK,LDH level and IL-1 β,TNF-α were significantly decreased (P＜0.05,P＜0.01,P＜0.01,P＜0.05),IL-10 had no statistical significance (P＞0.05),a significant increase in IL-10/TNF-α(P＜0.05),the level of MDA of gastrocnemius muscle decreased significantly (P＜0.05),the level of GSH and SOD,CAT and GSH-PX were significantly increased (P＜0,01,P＜0.01,P＜0.05,P＜0.01).Conclusion Acer truncatum oil can significantly reduce oxidative stress and inflammatory reaction caused by the exhaustive exercise,which has a protective effect on skeletal muscle.

0.05,there were not significant differences in two groups.In the hypothyroidism group,the characteristics had been improved,such as myxedema、feeling of cold,lowing-sweat、alopeda,The function of thyroid and heart rate had been improved。To compare with matched control,there were significant difference P

Objective To explore the relationship between the emotional labor and personality of the health care workers. Methods 168 health care workers in 6 hospitals of Shandong province were surveyed to complete the Emotional Labor Scale and the revised NEO personality inventory(NEO-PI-R). Results The first strategy of emotional labor that workers usually used was natural acting (5.17±1.15),the next one was deep act-ing (4.74±0.78) and the last was surface acting(3.88±1.21). The correlation analysis showed that the extro-version had a negative correlation (r=-0.20, P < 0.05) with the surface acting and a positive correlation (r=0. 31, P<0.01) with the deep acting. The agreeableness had a positive correlation (r=0.22, P < 0.05) with the nat-ural acting. The conscientiousness had a positive correlation (r=0.25, P<0.05) with the deep acting. The extro-version had significant negative influence on surface acting and significant positive influence on deep acting. The a-greeableness had positive significant effect on natural acting and the conscientiousness had positive significant effect on deep acting. Conclusion Personality influenced the emotional labor and gender,age,work tenure and ed-ucational had significant effect on emotional labor.

ObjectiveTo screen for proteins related to invasive and metastatic potential of ovarian cancer.MethodsThe nude mice were intraperitoneally injected with the human ovarian cancer 3AO cell line.The metastatic tumors were isolated and cultured in vitro.The cell lines were established from tumors and transplanted intraperitoneally for several passages.Invasive potential of cell lines was evaluated by cell adhesion and matrigel invasion assays.Lysates from different cell lines with high or low invasive potential were analyzed by surface enhanced laser desorption/ionization-time of flight-mass spectrometry(SELDI-TOF-MS)and protein-chip array technology.The proteomic fingerprint patterns were compared. ResultsThe subclones 3AOF3 and 3AOF4 were isolated from repeated passaging in vivo and vitro,and showed higher invasive capacity than 3AO cells.By comparing their proteomic fingerprints,9 distinct proteins (3494,3831,8121,8293,9123,9332,10 184,15 436 and 17 134 at m/z value) were found,which were expressed only in 3AO cells.On the contrary,1 distinct protein (4360 at m/z value) was expressed only in 3AO F3-4 cells,and 2 distinct proteins (1842 and 3145 at m/z value) were expressed only in 3AO F2-4 cells.Additionally,6 distinct proteins (1236,5476,5649,6575,6897 and 7016 at m/z value) and a mass cluster (13 794 and 14 027 at m/z value)were expressed in all 3AO and 3AOF1-4 cells,while the amount of protein was different.ConclusionBy comparing the proteomic fingerprints of isogenic ovarian cancer cells with different invasive potential,a group of proteins are identified which are correlated with high invasive and metastatic potential.

Objective To study the role of coping styles in the relationship between coping resources and psychosomatic health in patients with asthma. Methods 184 adult patients with asthma were investigated with General Serf-Efficacy Scale (GSES), Internality, Powerful Others, and Chance Scale (IPC), Social Support Rat-ing Scale (SSRS), Defensive Style Questionnaire (DSQ), Trait Coping Style Questionnaire (TCSQ) and Short Form 36 Health Survey Questionnaire (SF-36). Results Significant correlation between self-efficacy (r=0.356),locus of control(Ir=0.208, Pr=0.341, Cr=-0.349) ,subjective(r=0.201) and objective(r=0.189)social support, immature and middle ( r = - 0. 257 ) defensive styles and coping styles ( PCr = 0.231, NCr =-0.505 ) with Mental Component Summary (MCS) was found (all P <0.05), but Physical Component Summary (PCS) was only related to self-efficacy( r=0. 175) ,chance(r=0.150),immature(r=-0.256) and middle de-fensive Styles and negtive coping styles (all P < 0.05). PCS (t = - 5.189) and MCS (t = - 6. 758) could be predic-ted effectively by negative coping style (all P<0.01). Moreover, self-efficacy, powerful others and the use of so-cial support could had an effect on the health of patients through negative coping style. Conclusion Negative coping style could not only have a direct effect on the health of asthma patients but also be a mediator between self-efficacy, locus of control, social support and the health of patients.

Objective To observe the effects of kidney aquaporin-2 (AQP2) expression in Lung-Qi deficiency model rats and provide experimental evidence for the traditional Chinese medical theory that the kidney is related with the lung in charge of regulating the water passage. Method Twenty healthy rats were divided into the model group and the control group. The method of immunohistochemistry and Western blot was used to determine the expression of AQP2 in kidney. RT-PCR was applied to analyze the expression of AQP2 mRNA in lung and kidney tissues. Result The expression of AQP2 and AQP2 mRNA in kidney in model group was higher than in the control group. Conclusion The expression of AQP2 in kidney was higher in Lung-Qi deficiency model rats. One of the substance basis of "lung in charge of regulating the water passage" may be related to the change of AQP2 expression in the kidney tissues.

Objective: To discuss the application value with two-dimensional ultrasound speckle tracking technique in the evaluation of left ventricular function in patients with OSAS. Methods: Selected 110 cases of hospital patients with OSAS in recent years, used STI technical analysis of left ventricular myocardium using different directions overall resilience, Left ventricular torsion and systolic mitral annular midpoint of the maximum displacement (MAD) and left ventricular ejection fraction (LVEF) relationship, performed using SPSS 17.0 statistical correlation analysis. Results: MAD had a significant correlation with LVEF(r=0.81, P<0.01); GLS(r=-0.74, P<0.01),GRS GRS(r=0.57, P<0.01),GCS (r=-0.55, P<0.01) and LVtw(r=0.65, P<0.01)were significantly associated with LVEF;MAD and GLs(r=-0.69, P<0.01),GRs (r=0.49, P<0.01), LVtw (r=0.45, P<0.01)were significantly associated;MAD and GCs (r=-0.39, P<0.05)with a negative correlation. Conclusion:STI resilience through the myocardium, ventricular torsion and systolic mitral annular maximum displacement at the midpoint of the analysis, can accurately evaluate left ventricular longitudinal, radial and torsional motion synchronization, highly correlated with left ventricular ejection fraction, good consistency, with a very broad application prospects.

Objective To analyze the clinicopathologic features,prognosis and influencing factors of invasive cervical adenocarcinoma.Methods Clinical and pathologic data of 96 cases of invasive cervical adenocarcinoma and 100 cases of squamous carcinoma of the cervix treated in Shanxi Cancer Hospital from years of 1998 to 2007 were retrospectively analyzed.Results The morbidity of cervical adenocarcinoma was showing the arise and low-age trend.The overall 5-year survival rate was 60.4 ％,The overall survival rates of patients with stage Ⅰ,Ⅱ,Ⅲ,and Ⅳ were 82.9 ％,51.5 ％,13.3 ％ and 0,respectively.Ocervical adenocarcinoma had many prognosis-related factors:clinical stage,lymmphatic spread,vascular invasion,volume of tumor,histologic grade.Multivariate analysis showed that clinical stage,lymph nodal metastasis and vascular invasion were independent prognosis-related factors.However,whether ovary was reserved,it was irrelative to prognosis.Conclusion The morbidity of cervical adenocarcinoma is showing the arise and low-age trend.Earlier diagnosis is extremely important for improving the survival rate of cervical adenocarcinoma,the reserved ovary doesn't influence the overall 5-year survival rate for young women with early cervical adenocarcinoma.

Objective To investigate the changes of intestinal mucosal barrier function and protection of alanyl-glutamine(Alm-Gln) dipeptide during early stage of postthoracotomy in aged patients.Methods A prospective,randomized and controlled trial was conducted and 20 aged patients who underwent non-digestive thoracotomy were randomized into two groups,experimental group (intravenous administration of Aln-Gln dipeptide,0.5 g/(kg · d),for 4 days,n =10) and control group(equal amount saline as placebo,for 4 days,n =10).The indices of boby temperatures,heart rates,respiration and white blood cell count of all patients were daily recorded during administration.Serum concentrations of glutamine (Gln),D-lactate,diamine oxidase (DAO) and tumor necrosis factor-α(TNF-α) were measured before and after operation.Results There were no statistically significant differences in the patients' general information between experimental group and control group including age,gender and body weight.Plasma Gln concentration in postoperative 5 days was higher than that of pre-operation of experiment group ((478.32 ± 47.42) μmol/L vs.(372.67 ± 29.14) μmol/L,P =0.021).The plasma Gln level of control group at 5th day after operation was higher than that in pre-operation ((431.12 ± 42.27) μmol/L vs.(386.29 ± 19.73) μmol/L,P =0.017).The plasma level of Gln in experimental group was significantly higher than that in control group after operation((478.32±47.42) μmol/L vs.(386.29 ± 19.73) μmol/L,P =0.012).There were no significantly differences between the two groups in terms of the plasma level of DAO and D-lactate before operation (P ＞ 0.05).Meanwhile the levels of DAO and D-lactate in both group at 5th day after operation were significantly higher than that at before operation(DAO:(2.53 ±0.47) U/ml vs.(1.66±0.32) U/ml,P =0.003;D-lactate:(6.82 ±1.91) mg/L vs.(4.92 ±1.57) mg/L,P =0.024),and the levels of them in experimental group were significantly lower than that in control group(DAO:(1.10 ± 0.23) U/ml vs.(2.53 ± 0.47) U/ml,P =0.013 ; D-lactate:(4.87 ± 1.33) mg/L vs.(6.82 ± 1.91) mg/L,P =0.019).The concentration of TNF-α was significant increase in both two groups at first day after operation,but decreased at the third day.The concentration of TNF-α in experimental group at 5th day after operation was lower than that in control group ((6.89 ± 5.21) pg/L vs.(13.04 ± 4.46) pg,/L,P =0.003).The morbidity of systemic inflammatory response syndrome (SIRS) was significantly decreased in experimental group and the rate of SIRS was also lower than that in the control group(P ＜ 0.01).Conclusion Intestinal mucosal barrier function was damaged after thoracotomy in aged patients.Administration of Aln-Gln dipeptide could increase the level of serum Gln,protect the intestinal barrier and attenuate the systemic inflammatory response.Aln-Gln dipeptide can be used to help aged patients recover rapidly.

Objective To study the optimal dose of esmolol for maintaining cardiovascular stability in patients with hypertension during tracheal extubation. Methods In post-anestheisa care unit, hypertensive patients after general anesthesia meeting the extubation criteria were included. Patients were divided into 2 groups according the age: group Ⅰ (>65 years old for the elderly hypertensive, 21 cases), and groupⅡ(≤65 years old for the non-elderly hypertensive, 22 cases). All the patients received esmolol bolus before sputum suction and tube extraction, and the tracheal extubation were extubated 2 minutes after esmolol bolus. The systolic blood pressure, diastolic blood pressure and heart rate were was recorded before tracheal extubation, 2 min after esmolol bolus, at the time of sputum suction extubation, 1 min after tracheal extubation, 3 min after tracheal extubation and 5 min after tracheal extubation. Esmolol dose was determined by the up and down method. Initial dose was 0.5 mg/kg, in accordance with the arithmetic dose (0.2 mg/kg) increasing or decreasing progressively. In negative results (the systolic blood pressure at extubation or 5 min after extubation ≥ 20% of the base, or the systolic blood pressure at sputum suction extubation>180 mmHg, 1 mmHg=0.133 kPa) esmolol dose increased progressively, and in positive results (the systolic blood pressure at extubation or 5 min after extubation<20%of the base) esmolol dose decreased progressively. When the crosspoint (from positive to negative result) reached 6, the study was terminated. Results The median effective doses of esmolol for maintaining cardiovascular stability in groupⅠand groupⅡwere (0.6 ± 0.1) and (0.8 ± 0.1) mg/kg. Conclusions Esmolol can maintain cardiovascular stability in patients with hypertension during tracheal extubation. Median effective dose decreases in older hypertensive patients.