Objective To evaluate the value of HE4 ,CA125 in the diagnosis of epithelial ovarian cancer (EOC) .Methods A to‐tal of 54 samples with epithelial ovarian cancer ,64 suspicious benign pelvic mass ,and 60 health controls were consecutively enrolled in this study .Results Good diagnostic performance in discriminating benign from EOC patients was obtained for CA125 and HE4 . Serum level of HE4 of the patients in the three group were 189 .94 pmol/L ,56 .74 pmol/L and 46 .36 pmol/L .Levels of CA125 were 89 .39 U/L ,45 .11 U/L and 34 .24 U/L .There were stastically significant differences between the groups(P<0 .05) .The ser‐um HE4 and CA125 levels of the patients with ovarian cancer at stage Ⅲ ,Ⅳ(HE4=236 .25 pmol/L ,CA125=206 .35 U/L) were significantly higher than the cases at stage Ⅰ ,Ⅱ(HE4=96 .36 pmol/L ,CA125=67 .8 U/L P<0 .05) .Benign pelvic mass as con‐trol ,the specificity (SP) was 86 .6% ,sensitivity (SN) was 82 .6% ,positive predictive value and negative predictive value of the ser‐um HE4 were 85 .71% ,71 .67% ,73 .84% ,and 84 .31% respectively .SN of CA125 was 86 .67% ,higher than that of HE4 (71 .67% ,P<0 .01) .The area under curves (AUC) of HE4 was 0 .87 higher than that of CA125 (0 .81) ,(P<0 .01) .Combing de‐tection of ovarian cancer was higher than that of HE4 and CA125 alone .SP was 95 .15% ,negative predictive value was 92 .13%(P<0 .01) .Conclusion Overall ,the level of serum CA125 and HE4 increase significantly ,which showes well diagnostic perform‐ance to EOC from benign diseases .The combined detection of CA125 and HE4 could improve the diagnostic power in cervical cancer prominently ,so it has great reference value in the diagnosis of epithelial ovarian cancer .

Objective To detect the level of plasma superoxide dismutase(SOD) in patients with lung cancer and effect of chemotherapy(CGM + CBP) on it,and to investigate the role of SOD in the development .of lung cancer. Methods Xanthine oxidation enzyme was availabe to detect the level of plasma SOD in 30 cases of normal control subjects and 29 cases of patients with lung cancer before and after first, second, third cycle of chemotherapy. Resuits The levels of plasma SOD in lung cancer after chemotherapy were lower than those in the control group(P＜0.01). The level of plasma SOD in lung cancer after first and second chemo-cycle was higher than that before, but lower than that of control group(P＜0.01), the differences between after third chemo-cycle and control group were not significant(P＞0.05 ). The differences in squamoun cell carcinoma and adenocarcinoma before chemothrapy and after were not significant (P＞0.05 ). The differences in squamoun cell carcinoma and adenocarcinoma and adenosquamoun carcinoma before chemotherapy and after were highly significant (P＜0.01 ). The differences in each chemo-cycle were highly significant (P＜0.01). Conclusion SOD played an important role in growth, regulation and treatment of lung cancer,and it might be a clinical index in lung cancer.

Objective To investigate the clinical curative effect and safety of ambrocol oral liquid combined with pidotimod oral liquid in the treatment of children with mite allergic asthma.Methods 54 cases of children with mite allergic asthma from December 2014 to December 2015 were selected and randomly divided into the control group and experimental group,27 cases in each group.On the basis of routine treatment,the control group was given pidotimod oral solution 10mL,once a day,oral; on the basis of the control group,the experimental group was treated with ambrocol oral solution 10mL,twice a day,oral.Interleukin-2(IL-2),interleukin-4(IL-4),immunoglobulin E(IgE)levels,the efficacy and safety of the two groups were measured before and after treatment.Results After treatment,compared with the control group,the serum levels of IL-2 were higher,and the levels of IL-4、IgE were lower in the experimental group,the difference was statistically significant(P<0.05),the treatment efficiency of the experimental group(92.59％)was significantly higher than that of the control group(81.48％),the difference was statistically significant(P<0.05).Conclusion The ambrocol oral liquid combined with pidotimod oral liquid can significantly improve the serum level of IL-2,and reduce the serum level of IL-4,IgE in children with mite allergic asthma,improve clinical efficacy.

Objective To study the epidemiological characteristics of the acute toxicosis cases in a general hospital of a metropolis. Methods The prospective study was performed from Jan. 1st, 2005 to Dec. 31st, 2006 in acute toxicosis patients admitted during that period. The data analyzed included sex, age, occupation, onset time, poisoning cause, variety of poisons, way of poisoning, emergency diagnosis, emergency treatment, etc. Results There were 315 poisoning patients treated during the study period, accounting for 2.33‰ of the total patients visiting in the emergency department. Among these poisoning patients, the sex ratio (males vs females) was 1∶0.84. The age of most of the patients ranged from 20 to 29 (35.9%) years old. Alcohol was the major cause of poisoning (38.4%), followed in order by poisoning due to drugs (20.6%), food poisoning (18.1%), chemical poisoning (10.2%) and pesticide poisoning (5.7%). There was predominant time of the occurrence of poisoning in a year, but it was more frequent in January, February, June, July, November and December. 85.1% of patients were poisoned by oral route. The occupation of patients was mainly technical personnel (21.0%), followed in order by farmers and the laborers, self-employed businessmen, persons taking care of household chores and the unemployed, etc. 98.1% of patients survived in the hospital. Mortality rate was 1.9%. Conclusions Young persons and technical personnel constitute the high-risk group of patients with poisoning. The main poisons were alcohol, drugs, food and other chemical poisons. The main route of poison intake was the digestive tract. There is no obvious seasonal regularity.

Objective To evlauate the clinical feasibility and efficacy of laparoscopic right hemicolectomy in the treatment of colon carcinoma .Methods 100 patients with rightcolon carcinoma were divided into 2 groups,50 ca-ses in each group .The control group received open surgery ,and the observation group was treated by laparoscopic right hemicolectomy .The clinical effect and side effect of the two groups were compared .Results The patients of the two groups were all successfully operated .The intraoperative blood loss ,postoperative anal exhaust time ,postoperative hos-pital stay,incidence rate of postoperative complications in the observation group were (75 ±42)mL,(24.8 ±1.3)h, (7.5 ±2.0)d,4.0%,respectively,which were significantly better than those of the control group [(100 ±50)mL, (64.9 ±1.8)h,(12.1 ±3.4)d,10.0%](χ2 =5.02,9.28,11.70,9.87,all P<0.05);Conclusion The laparo-scopic right hemicolectomy in the treatment of rightcolon carcinoma is safe and feasible ,and the curative efficacy is outstanding .It can obviously improve the prognosis of patients ,which should be popularized and applied in clinical .

Objective To explore the feasibility and clinical effect of single-incision laparoscopic surgery(SILS) in the treatment of gastrointestinal stromal tumors (GIST).Methods Clinical data of 13 GIST patients who underwent SILS resection [tumor diameter (1.3 ± 0.5) cm] were retrospectively analyzed.Surgical method was summarized and the clinical outcome was evaluated.Results All patients were successfully implemented without open conversion.The incision length was (3.8 ± 0.6) cm.Operation time was (100.0 ± 34.6) min.Intraoperative blood was (70.0 ± 45.5) ml,hospitalization time was 1 ～ 4d.There were no intraoperative or postoperative complications,such as secondary haemorrhage,anastomotic leakage or obstruction.The patients were favorably healed.Conclusion Application of SILS in treatment of GIST is safe with small trauma,fast postoperative rehabilitation,and early curative effect is satisfactory.

Objective To explore the clinical value of laparoscopic hepatectomy in liver tumor surgery.Methods The clinical data and follow-up results of 50 patients with liver tumors treated by laparoscopic hepatectomy were retrospectively analyzed.Results Laparoscopic liver resection was successful in 30 cases of primary liver cancer,10 cases of hepatic hemangioma,and 10 cases of other benign liver space occupying lesions (liver vascular smooth fatty tumor,focal nodular hyperplasia,hepatic abscess).Conclusion Laparoscopic hepatectomy as a minimally invasive treatment method is applicable to all parts of liver tumor surgery,and trauma is small,recovery is quick,and it is safe and feasible,and clinical effect is reliable.

Objective To investigate the expression of Skp2 and Cx43 in cervical carcinoma and to study their relationships and clinical significance. Methods The expression of Skp2 and Cx43 was examined by immunohistochemical method in 48 cases of cervical carcinoma tissue, 84 cases of cervical intraepithelial neoplasia (CINⅠ - Ⅱ 45 cases, CINⅢ 39 cases), 28 cases of chronic cervicitis. Results Skp2 expression in cervical carcinoma was higher than that in the CINⅠ - Ⅱ and chronic cervicitis (P <0.05), Cx43 expression was lower in cervical carcinoma than that in the CINⅢ, CINⅠ - Ⅱ and chronic cervicitis (P <0.05). The expression of Skp2 was correlated with histological differentiation and lymph node metastasis (P <0.05). The expression of Cx43 was correlated with the lymph node metastasis (P <0.05). Besides, the expression of Skp2had a negative correlation with that of Cx43. Conclusion Skp2 and Cx43 may play an important role in the genesis and development of cervical carcinoma.

Objective To explore the causes and prevention methods of postoperative anastomotic leakage after colorectal cancer laparoscopic-assisted surgery.Methods 100 patients with colorectal cancer underwent laparoscopic-assisted surgery,the data was collected and retrospectively analyzed.Results All patients were successfully done,there were 5 cases with anastomotic leakage (5％).There were significant correlations of anastomotic leakage with too strong anastomotic tension,insufficiency anastomotic blood supply,tumor location,size,operation time,preoperative radiotherapy,gender,age,obesity,diabetes,hypoalbuminemia,and so on.Conclusion The laparoscopic-assisted surgery of colorectal cancer has a curative efficacy,with the continuous improvement of laparoscopic technique and operation,as long as the proper measures were taken,the occurrence of anastomotic leakage can be prevented and reduced.

Objective To explore the expression of nemo-like kinase (NLK) in primary hepatic carcino-ma (HCC) and its clinicopathological significance. Methods The expression of NLK was detected in 136 HCC samples by Immunohistochemistry. Results NLK expression was significantly up-regulated in HCC specimens compared to corresponding normal liver tissues. High expression of NLK was significantly associated with Ed-mondson-steiner grade, tumor size and number of tumor nodules (all P < 0.05). There was positive correlation between NLK and proliferation marker Ki-67 (P < 0.01). Kaplan-Meier analysis showed the high expression NLK group was significantly associated with poor overall survival and disease-free survival (all P < 0.001). Univariate analysis showed that the high expression NLK was associated with poor prognosis (P < 0.001). Multivariable Cox regression analysis suggested that the expressions of NLK and Ki-67 , Edmondson-steiner grade , metastasis , tu-mor size and number of tumor nodules were independent prognostic indicators for HCC. Conclusions NLK was markedly upregulated in HCC specimens, and it might be an independent prognostic marker for HCC. NLK might play an important role in tumorigenesis, progression and prognosis of HCC.