Small conductance Ca 2+ activated potassium channels ( SK channels) are potassium selective , voltage independent, and Ca 2+ high sensitivity. SK channels are widely distributed in neurons. SK underlie the slow afterhyperpolarization , which regulate the fire of neurons by spike frequency adaptation. Three mammalian SK channels (SK 1, SK 2, SK 3) have been cloned . Ca 2+ , CaM and neurotransimitters involve in the gating of these channels. The changes of these channels may result in memory, neuromuscular disorder and schizophrenia.

Objective To investigate the clinical value of spiral CT in diagnosis of fracture of tibia plateau, to provide a reference for clinical treatment.Methods 50 cases of tibial plateau fracture were selected,they were diagnosed with X -ray and diagnostic multislice spiral CT three dimensional reconstruction scan respectively.The fracture type and so on of the two groups were compared.Results 50 patients were diagnosed as 14 cases of type Ⅰ, type Ⅱ 10 cases,Ⅲ type 6 cases,Ⅳ type 7 cases,Ⅴ type 5 cases,Ⅵ 8 cases,CT three -dimensional reconstruction diagnosis accuracy rate was 100.00%.X -ray diagnosis was accurate in 41 cases,diagnostic accuracy rate was 82.00%,the difference of two diagnostic ways was statistically significant (χ2 =8.657,P =0.000).All patients were not detected with significant artifacts,the line was good,the fracture line was blurred and visible fracture callus formation surgical outcome was good.Conclusion Spiral CT diagnosis in tibial plateau fracture has high accuracy,through clear and intuitive display morphological characteristics of the patients improves anatomical repair treatment,it is worthy of clinical application.

OBJECTIVE:To discuss the management tactics and developmental prospects of fair-price drug stores in China.METHODS:To analyze the advantages and disadvantages of fair-price drug stores and countermeasure.RESULTS&CONCLUSION:Development of fair-price drug stores need not only support of governments but also the improvement of drug and service quality by strengthening physical distributions,renewing management methods and bringing up specialists.

Objective:To isolate and identify the periodontal ligament stem cells.Methods:Periodontal ligament tissues were digested with a mixture of collagenase and Dispase,then the periodontal ligament stem cell clones were isolated by limited dilution method.The transmission electron microscopy(TEM),flow cytometery and immunohistochemistry procedure were employed to study the ultrastructure,cell cycle and surface marker of the cloned cells.Results:The obtained cells showed the characteristics of undifferentiated morphology at ultrastructural level.94.4% of the cells were in phase G_0/G_1.The cells were Vimentin,STRO-1,ON and BSP positive.Conclusion:Cloning incubation may be the effective way to isolate and purify the periodontal ligament stem cells.

Abstract Objective:To obtain recombinant vaccinia viruses expressed HIV-lcn subtype B gag and gag-hIL-2 chimera gene,evaluate theimmune effects in experimental mice by immunization with HIV-1 DNA vaccine,observe the immune adjuvant effect of IL-2.Metbods: HIV0-1gag and gag-hIL-2 chimera gene were inserted downstream of the combined promotor of pJ38 vector respectively and recombinant vaccinia virus-es were selected by plaque essay. Expression products were examined by SDS-PAGE and Western blot.Immune response of immunized Balb/cmice was evaluated by lymphocyte transformation test,CTL quantity change of T lymphocyte of CD4+、CD8+ and the level of serum antibody.Re-sults:Recombinant vaccinia viruses vJ38gag and vJ38gag-IL-2 were obtained and the expression were confirmed by SDS-PAGE and Western-blot. Recombinant vaccinia viruses show good immune effect when immuned with HIV-1 DNA vaccine.The best strategy for immunization is therVV-primed and HIV-1 DNA vaccine-boosted group and threer times of immunization were suggested to get effective results compared withvJ38gag an incressed immune response was induced by vJ38gag-IL-2. Conclusion: HIV-1 Gag protein and IL-2 protein can be expressed invJ38gag and vJ38gag-IL-2 respectively. IL-2 show good effect to ha sued as immune adjuvant.

AIM To study the effects of propafenone on Kv 4 2 and Kv4 3 potassium current. METHODS Whole cell patch clamp technique was used to record changes of Kv4 2 and Kv4 3 potassium currents. RESULTS ① Propafenone redu ced the amplitude of Kv4 2 and Kv4 3 currents, in a concentration-dependent, IC 50 was 10 3 and 71 ?mol?L -1 respectively. ② Propafenone inhibi ted Kv4 2 and Kv4 3 current inactivation, 10 ?mol?L -1 propafenone acce lerates Kv4 2 current inactivation constant from (38 9?2 1) ms to (9 9?1 8) ms; and shifted the steady state inactivation curves of Kv4 2 current to negative potentials. 100 ?mol?L -1 Propaf enone accelerates Kv4 3 current inactivation constant from (144 8?20 8) ms t o (18 5?2 8) ms; and shifted the steady state inactivation curves of Kv4 2 c urrent to negative potentials. ③ propafenone inhibited Kv4 2 and Kv4 3 curren t activation, 10 ?mol?L -1 propafenone shifted the steady state activati on curves of Kv4 2 current to negative potentials. 100 ?mol?L -1 propafe none shifted the steady state activation curves of Kv4 2 current to negative po tentials. CONCLUSION Propafenone, maybe, also exerts its effects via blocking Kv4 2 and Kv4 3 current to antiarrhythmia.

AIM To study the effects of H 2O 2 on transient outward potassium current METHODS Whole cell patch clamp technique was used to record changes of I A in the cultured rat hippocampal neurons RESULTS After exposure to 30 ?mol?L -1 H 2O 2 for 12 h (1) decreased I A current density from (53 8?15 8) PA?PF -1 to (18 5?6 2) PA?PF -1 ( n=10,P

Objective To explore the expression of nemo-like kinase (NLK) in primary hepatic carcino-ma (HCC) and its clinicopathological significance. Methods The expression of NLK was detected in 136 HCC samples by Immunohistochemistry. Results NLK expression was significantly up-regulated in HCC specimens compared to corresponding normal liver tissues. High expression of NLK was significantly associated with Ed-mondson-steiner grade, tumor size and number of tumor nodules (all P < 0.05). There was positive correlation between NLK and proliferation marker Ki-67 (P < 0.01). Kaplan-Meier analysis showed the high expression NLK group was significantly associated with poor overall survival and disease-free survival (all P < 0.001). Univariate analysis showed that the high expression NLK was associated with poor prognosis (P < 0.001). Multivariable Cox regression analysis suggested that the expressions of NLK and Ki-67 , Edmondson-steiner grade , metastasis , tu-mor size and number of tumor nodules were independent prognostic indicators for HCC. Conclusions NLK was markedly upregulated in HCC specimens, and it might be an independent prognostic marker for HCC. NLK might play an important role in tumorigenesis, progression and prognosis of HCC.

Objective:To investigate the effects of enamel matrix proteins(EMPs) on proliferation and alkaline phosphotase (ALP) activity of human dental follicle cells(HDFCs) in vitro. Methods:HDFCs were dissected and cultured by digestion with bacterial collagenase. MTT method and enzyme kinetics method were used to examine the proliferation and ALP activity of HDFCs treated with EMPs.Results:EMPs at 25~200 mg/L increased the proliferation of HDFCs and EMPs at 100 mg/L showed the strongest effects in 7-day-culture.EMPs at 100 mg/L increased ALP activity of HDFCs in 3- and 7-day cultures.Conclusion:EMPs may stimulate the proliferation and the ALP activity of HDFCs.

Objective To study the effect of glycerin fructose combined with mannitol in the treatment of postoperative swelling of extremities fractures.Methods In Yiwu Central Hospital from February 2015 to February 2017 treated 100 cases of limb fracture surgery patients as the research object in the course of the study, were randomly divided into control group and experimental group two were 50 cases each.The patients in control group were treated with mannitol after fracture surgery, and the patients in the experimental group were treated with glycerol fructose and mannitol.Comparative analysis of the experimental group and control group of patients with limb swelling degree.Results After different treatment, the experimental group of patients with adverse reactions occurred in 0 cases, the probability was 0.0%, the control group of patients with adverse reactions in the number of cases in the case of the number of patients with adverse reaction probability of 10.0%, the number of patients with adverse reactions in the control group was 5.As a result, the incidence of adverse reactions in the experimental group was significantly lower than that in the control group, with statistical difference(P<0.05).In the experimental group, 6 days after surgery, on the 7 day, the 8 day and the 9 day, the forearm circumference was significantly lower than that of the control group, with statistical difference(P<0.05).On the 8 day after surgery and after 8 days, the leg circumference of the experimental group was significantly lower than that of the control group, with statistical difference(P<0.05).Conclusion The clinical effect of glycerol and fructose combined with mannitol in treatment of limb swelling after surgery for fracture, high safety, reduce the probability of occurrence of adverse reactions to a great extent, is further applied in clinical significance.