Objective To study the expression of MMP-9 in epatocellular carcinoma (HCC) and the relationship between its expression and clinical pathological characteristics.Methods Specimens taken from 67 fresh resected HCC and 16 normal liver tissues were selected.Tissue sections were stained by EnVisionTM immunohistochemistry with anti-MMP-9 monoclonal antibodies.The expression of MMP-9 in HCC and the relationship between its expression and clinical pathological characteristics were analyzed with x2test,t test and rank-sum test.Results The positive ratio of MMP-9 in HCC tissues was 58.21％,it was obviously higher than that of normal liver tissues (12.5％,x2 =10.80,P ＜ 0.01).The expression of MMP-9 in the metastatic HCC tissues was significantly higher than that in the non-metastatic HCC tissues (40.18 vs 26.38,P ＜0.01).Conclusions The positive ratio of MMP-9 expression revealed that the expression of MMP-9 increased obviously in the HCC,and that its expression in the metastatic HCC tissues significantly increased relative to the non-metastatic tissues.It suggests that MMP-9 might decompose and destroy the extracellular matrix and cell membrane around HCC cell surface,and then promote invasion and metastasis of HCC.

Objective To investigate the curative effect of fast track surgery laparoscopic surgery and traditional laparotomy in elderly patients over 70 years old with rectal cancer and the difference between the two groups was analyzed,and explore the practical value and the advantages in fast track surgery (FTS) laparoscopic surgery in the patients older than 70 years with colorectal cancer.Methods This study was a retrospective analysis,in accordance with the requirements of the laparoscopic group and the laparotomy group,the clinical data of 100 patients over 70 years old with rectal cancer were randomly sampled who were admitted to the second people's Hospital of Hunan province in April 2010-2016 November.Fifty patients underwent FTS laparoscopic assisted rectal cancer,which was set as the experimental group;the other 50 patients underwent conventional open surgery were set as the control group.The clinical data of two groups were compared with preoperative,intraoperative and postoperative related indicators.Results No significant difference was found in the two groups of patients in surgery (P ＞ 0.05).The operative time of experimental group was [(190 ± 35.2) min] longer than the control group [(160.5 ± 29.3) min] (P ＜ 0.05).The incidence of hypercapnia in the experimental group was higher than the control group (P ＜ 0.05).The intraoperative bleeding of the experimental group [(90 ± 40) ml] was significantly less than the control group [(130 ± 60)ml,P ＜0.05].The postoperative exhaust time of the experimental group [(3.01 ± 0.98)d] was shorter than the control group [(3.98 ± 1.38)d,P ＜0.05].The lymph node number of the experimental group (12.86 ±4.87) was not significantly different from control group (13.12 ±4.69,P ＞ 0.05).The hospitalization time of the experimental group [(15.68 ±5.10) d] was significantly shorter than the control group [(20.31 ± 6.78) d,P ＜ 0.05].The wound infection rate of experimental group was significantly lower than the control group (P ＜ 0.05).The anastomotic leakage in experimental group was not significantly different from control group (P ＞ 0.05).No significant difference in anastomotic stenosis was found between the experimental group and the control group (P ＞ 0.05).Conclusions The concept of FTS laparoscopic radical resection of rectal cancer has the advantages including less bleeding,quicker recovery,and less complications.It is safe and feasible and worthy of clinical application.

Two thousands six hundreds and forty-four patients of malignant tumors of the breast were diagnosed and treated in our hospital from 1956 to 1990. Twenty-one cases of them were diagnosed mammary sarcoma by pathology in 0. 8% in contcmporary malignant tumors of the breast. The axillary lymph nodes of 6 cases in this group are clinically enlarged in 28. 6%, all of them were proved benign through mieroscopie examination. The age of the patient of mammary sarcoma is younger than that of the patient of mammary careinoma in the group. The patient's age over forty-five years and more than one year course have a hetter prognosis. Mammary sarcoma metastasizes by blood stream or local infiltrate, rarely metastasizes to regional lymph nodes. Mammary sarcoma is treated by removal of the tumor and simple mastectomy. The value of radiotherapy will be futher explored by practice.

Smart library is an advanced stage in the development of library and service is the key content in aca-demic library.The service model of smart library was described from the angle of information service, cloud service and Library 3 .0 with the connotation of smart library and the service function of academic library as the starting points.in an attemp to provide reference for academic libraries to establisht their target of smart library construction.

Cognitive function is an important part of non-motor function of the cerebellum. The cerebellum is involved in cognition and has a variety of cognitive functions, including working memory function, language function, spatial cognitive function (spatial processing and spatial memory), and temporal cognitive function (time perception and time processing), etc. This article reviewed recent advances in these aspects of cognitive function of the cerebellum.

Objective To investigate the infection status of nanobacteria on patients with upper urinary calculi and healthy sub-jects ,and analyze the role of nanobacteria in the formation of upper urinary calculi .Methods The serum ,urine and calculus of 42 patients with upper urinary calculi were investigated by polymerase chain reaction (PCR) and by bacteria cultivation with 10% γ-FBS and PMBI 1640 .The resulting PCR products were sequenced for further comparison with the reported sequence in gene bank . The serum and urine from 30 healthy adults were used as controls .Results After 4 to 6 weeks′culture ,the white or yellow precipi-tate was visible at the bottom of the tube .The positive rate of PCR was 90 .4% in calculous patients urine and 6 .7% in healthy a-dults urine ,as the positive was 92 .8% and 6 .7% in serum .which there is significant difference (P<0 .01) .The positive rate of the nanabactria in urinary calculi was 95 .2% .The coincidence rate was 98 .72% between the PCR products and the reported sequence in gene bank .Conclusion Nanobacteria are widely existed in the serum ,urine and calculus of the patients with upper urinary calcu-li ,this indicate that the nanobacteria might be have the most important influence on the formation process of urinary calculi .

Objective: To analyze the present situation of the management of large-scale instruments in a medical school and give some reasonable suggestions. Methods: Through the research on the use of 85 large-scale instruments in our university, we tried to find out the methods of improving the utilization ratio of large-scale instruments. Results:There are many reasons of the poor management and low utilization rate of large-scale instruments in medical schools. Conclusion: Taking some measures could effectively improve the utilization rate of large-scale instruments in medical schools, such as simplifying the working procedure, solving the operating and repair expenses, strictly controlling argumentation, drawing up scientific evaluation and incentive measures and so on.

Objective To observe the clinical efficacy of Qihuang needle therapy in treating knee osteoarthritis.Methods Sixty knee osteoarthritis patients were randomly divided into treatment group and control group,30 cases in each group.The treatment group was given Qihuang needle therapy and the control group was given conventional acupuncture therapy.After treatment for 10 days,we evaluated the curative effect of the two groups by the scores of the simplified McGill pain scale (MPQ) and the Lysholm knee scoring criteria.Results (1) After treatment for 10 days,the symptoms of pain,swelling,stiffness,and inhibited bending and stretching of knee joints of the two groups were relieved.Pain rating index (PRI),visual analogue scale (VAS) scores and present pain intensity (PPI)of MPQ in the two groups were obviously decreased,while Lysholm knee scores were markedly increased(P ＜ 0.01 compared with those before treatment),and the effect of the treatment group was superior to that of the control group (P ＜ 0.05).(2) The total effective rate of the treatment group was 86.67％,and that of the control group was 66.67％,the difference between the two groups being significant (P ＜ 0.05).Conclusion Qihuang needle therapy is effective for the treatment of knee osteoarthritis,with the advantages of shorter treatment course,better therapeutic effect and being more practical.

To analyze the influence of related factors on recurrent hemorrhage after operation of hypertensive intrac-erebral hemorrhage,and investigate the countermeasures against recurrent hemorrhage. 124 patients with surgical treated hypertensive intracerebral hemorrhage were analyzed retrospectively. Logistic regression analysis was applied to analyze the function of various possible factors that might induce recurrent hemorrhage. The incidence of recur-rent postoperative hemorrhage was 16. 1% . Univariate logistic analysis disclosed that timing of operation,the hem-orrhage site and coagulation factor disorders,difficulties met during the operation and inadequate hemostasis,and postoperative blood pressure fluctuation were significantly related with recurrence of hemorrhage(P < 0. 05). Multi-variate logistic regression analysis showed that timing of operation,difficulties met during the operation and inade-quate hemostasis and postoperative blood pressure fluctuation were risk factors of recurrent hemorrhage(P < 0. 05). The optimal operative time,strict hemostasis in operation and appropriate postoperative blood pressure control are important measures in preventing recurrent hemorrhage.

Objective To evaluate the clinical effects of Kangfuxin solution as the auxiliary treatment of chronic periodontitis.Methods 41 patients with chronic periodontal diseases were randomly divided into test group and control group.Following initial therapy,Kangfuxin solution was applied to the test group,but the control group received no drug.The bleeding on probing(BOP)was observed before treatment and 1 week after treatment.Results Before treatment.there was no significant difference in the index between the two groups.After initial therapy 1 week,both groups showed improvement in their examined index(all P＜0.01).The results of the test group were better than those of the control group,and the differences were statistically significant(P＜0.01).Conclusion Kangfuxin solution as the auxiliary treatment of periodontitis could effectively relieve the clinical symptoms and gain good clinical effect.It was worth to be used widely.