Objective To evaluate the role of Src kinase in the induction and maintenance of spinal long-term potentiation(LTP).Methods The C-fiber evoked field potentials were recorded at the superficial layers of spinal dorsal horn at the lumbar enlargement.Results ① Genistein(200?mol/L) or PP2(100?mol/L),a selective Src kinase inhibitor,completely blocked LTP induction when administered at 30min prior to tetanic stimulation.② Genistein or PP2 reversed spinal LTP in a time-dependent manner.At 15min after LTP induction,Genistein(200?mol/L) or PP2(100?mol/L) reversed LTP completely.At 30min after LTP induction,however,the same concentration of Genistein or PP2 did not affect the spinal LTP.Conclusion Activation of Src kinase in spinal dorsal horn may be crucial for the induction and early-phase maintenance of LTP of C-fiber evoked field potentials.

Objective To explore the role of cyclooxgense-2(COX-2) in tetrahydrocannabinol(THC)-induced inhibition of long-term depression(LTD) in CA1 area in vitro.Methods The hippocampal slices were prepared at 24 h after intraperitoneal injection of ?9-THC(10 mg/kg) or COX-2 inhibitor NS398(10 mg/kg).Field excitatory post-synaptic potentials(fEPSP) were recorded in the stratum radiatum of the CA1 area in vitro to observe the effect of NS398,a selective inhibitor of COX-2,on the THC-induced inhibition of LTD and THC's effect on membrane excitability in pyramidal neurons by using the whole-cell patch clamp technique. Results ① Low-frequency stimulation(1 Hz,15 min) induced-LTD in CA1 area was significantly attenuated by ?9-THC.② ?9-THC did not affect the basal synaptic transmission and membrane excitability(including membrane resting potentials,input resistance and firing property).③ THC-induced inhibition of LTD was reversed by NS398.④ THC-induced inhibition of LTD was robustly impaired in COX-2 knockout mice.Conclusion THC-induced inhibition of LTD in CA1 area was mediated via COX-2.

TP5 and CsA were injected-intraperitoneally into groups of experimental mice, and NS was used for the contral group. Herpes simplex keratitis models were made subsequently by inoculation of the virus on the cornea. On the 4～6th day after inoculation, slit lamp examination showed that the corneal epithelial and stromal lesions in the TP5 group were significantly more severe than those in the NS group, whereas the group of CsA manifested no such differences. Among the 3 groups of mice, no differences were evident in the formation of corneal neovascularization, which all peaked on the 8th day. The conjuctival and palpebral lesions in the TP5 and CsA groups were also more severe than in NS group. Therefore, immunomodulators should be clinically used with care in accordance with the morbid varieties and conditions.

Pseudomonas aeruginosa was inoculated into corneal stroma of rabbits and the model of keratoconjunc tivitis was established. Empty liposomes were used in a control, group ofloxacin liposome and ofloxacin eyedrop groups as experimental ones. After treatment for six times conjunctival and corneal lesions were examinated. Swabs of conjunctival sac were cultured. Result: in conjunctiva, lesions in the two experimental groups were all significantly less than those in the control group on the 6th, 8th and 11th day. Lesions in the ofloxacin liposomes group were also significantly less than those in the ofloxacin eyedrop group on the6th day. In cornea, lesions in the ofloxacin liposomes group were significantly less than those in the control group on the 6 th, 8th and 11th day. Lesions in the ofloxacin eyedrop group were significantly less than those the control group on the 11th day. In the result of bacterium culture, the culture's positive rate of two experimental gronqs and control group had significant differences on the 6th day. On the 11th day, the positive rate the ofloxacin liposome groups and ofloxacin eyedrop groups increased, and in the ofloxaein liposomes group had a less increase. Conclusion: ofloxacin liposomes and ofloxacin eyedrops were effective in treatments of pseudomonas keratoconjuntivitis in the rabbit model, the effects of ofloxacin liposomes were better than that of ofloxacin eyedrops.

AIM To investigate the effects of idazoxan on the viability and DNA integrity of clonal BRIN-BD11 cells. METHODS MTS reduction was used to check the viability of BRIN-BD11 cells. FACS cell cycle analysis was employed to determine the effects of idazoxan on DNA integrity of BRIN-BD11 cells. RESULTS ①Idazoxan markedly reduced the viability of BRIN-BD11 cells and resulted in DNA damage in time-and dose-dependent manner.②Genistein, 5-HT 1A antagonist NAN190 and PKG inhibitor-KT5823 had no protective effects against idazoxan-induced damage of BRIN-BD11 cells. CONCLUSION Idazoxan has cytotoxic effect and induces apoptosis of clonal pancreatic ?-cells.

Objective To study the expression of MMP-9 in epatocellular carcinoma (HCC) and the relationship between its expression and clinical pathological characteristics.Methods Specimens taken from 67 fresh resected HCC and 16 normal liver tissues were selected.Tissue sections were stained by EnVisionTM immunohistochemistry with anti-MMP-9 monoclonal antibodies.The expression of MMP-9 in HCC and the relationship between its expression and clinical pathological characteristics were analyzed with x2test,t test and rank-sum test.Results The positive ratio of MMP-9 in HCC tissues was 58.21％,it was obviously higher than that of normal liver tissues (12.5％,x2 =10.80,P ＜ 0.01).The expression of MMP-9 in the metastatic HCC tissues was significantly higher than that in the non-metastatic HCC tissues (40.18 vs 26.38,P ＜0.01).Conclusions The positive ratio of MMP-9 expression revealed that the expression of MMP-9 increased obviously in the HCC,and that its expression in the metastatic HCC tissues significantly increased relative to the non-metastatic tissues.It suggests that MMP-9 might decompose and destroy the extracellular matrix and cell membrane around HCC cell surface,and then promote invasion and metastasis of HCC.

BACKGROUND: In the past, much attention was paid mainly to the body symptoms like focal neurological impairment in stroke, but the accompanied psychological symptoms were ignored easily.OBJECTIVE: To investigate the psychosocial factor in acute cerebrovascular disease and the mental state after the attack.DESIGN: Observational case-control study based on patients and healthy people.SETTING: A neurology department in a university hospital.PARTICIPANTS: Totally 55 patients with stroke hospitalized in Neurology Department of Hospital Affiliated to Medical College of Shaoxing Art and Science College and 35 healthy people taking physical examination from January 2001 to December 2003 in the hospital as controls.METHODS: Patients with acute cerebrovascular disease certified by CT/MR and people in control group took symptoms checklist-90(SCL-90) test within a month after attack.MAIN OUTCOME MEASURES: SCL-90 scores of the two groups.RESULTS: The condition of mental state in patients with acute cerebrovascular disease was worse than that in control group( P ＜ 0.01 ). There existed obvious physical and mental reaction. It was found in subsection analysis that the size and location of the focus had relationship with the degree of mental disorder after attack and it was easier to have mental disorder if the affection lied in left cerebral hemisphere.CONCLUSION: It is necessary to give psychological intervention in prevention and cure of acute cerebrovascular disease. Recognizing bad effect of stroke on psychoactivity may help to guide the treatment.

Objective To evaluate the role of EP_2 in synaptic transmission in dentate gyrus of hippocampus. Methods Field excitatory post-synaptic potentials (fEPSP) were recorded at the perforated path-granule neurons in dentate gyrus in vitro. Results ① Butaprost, an agonist of EP_2, enhanced the synaptic transmission in dentate gyrus and decreased the paired-pulse ratio, and these effects were reversed by bath application of AH6809 (EP_2 antagonist). ② Application of Forskolin alone or with AH6809 elevated the slope of fEPSP. ③ The Butaprost-induced responses were mediated via PKA, ERK and IP_3 signal pathways. Conclusion Multiple signal pathways were involved in the EP_2 activation-mediated enhancement of synaptic transmission.

Objective To explore the role of cyclooxgense-2 (COX-2) in tetrahydrocannabinol (THC)-induced inhibition of long-term depression (LTD) in CA1 area in vitro. Methods The hippocampal slices were prepared at 24 h after intraperitoneal injection of Δ~9-THC (10 mg/kg) or COX-2 inhibitor NS398 (10 mg/kg). Field excitatory post-synaptic potentials (fEPSP) were recorded in the stratum radiatum of the CA1 area in vitro to observe the effect of NS398, a selective inhibitor of COX-2, on the THC-induced inhibition of LTD and THC's effect on membrane excitability in pyramidal neurons by using the whole-cell patch clamp technique. Results ① Low-frequency stimulation (1 Hz, 15 min) induced-LTD in CA1 area was significantly attenuated by Δ9-THC. ② Δ~9-THC did not affect the basal synaptic transmission and membrane excitability (including membrane resting potentials, input resistance and firing property). ③ THC-induced inhibition of LTD was reversed by NS398. ④ THC-induced inhibition of LTD was robustly impaired in COX-2 knockout mice. Conclusion THC-induced inhibition of LTD in CA1 area was mediated via COX-2.

Objective To study the clinical efficacy of canalith reposition on benign paroxysmal positional vertigo(BPPV).Methods In the eighty patients with BPPV,the 45 cases with anterior semicircular canal were treated with Epley reposition method and the other 35 patients with the horizontal semicircular canal were treated with barbecue tumble method.The clinical efficacy was observed.Results The effective rate of the Epley reposition method for anterior semicircular canal patients was 95.5％ (43/45) and the effective rate of the barbecue tumble method for horizontal semicircular canal patients was 91.4％ (32/35).Conclusion The canalith reposition method treated with BPPV patients has high clinical efficacy,simple procedures,and no obvious adverse reactions.