Heterotopic ossification is the bone formation outside skeletal system, which can lead to serious motor dysfunction. This paper reviewed the definition, pathogenesis, incidence, clinical manifestations and diagnosis, advance in prevention and treatment of heterotopic ossification to investigate the effective method in prevention and treatment of heterotopic ossification.

BACKGROUND: In the past, much attention was paid mainly to the body symptoms like focal neurological impairment in stroke, but the accompanied psychological symptoms were ignored easily.OBJECTIVE: To investigate the psychosocial factor in acute cerebrovascular disease and the mental state after the attack.DESIGN: Observational case-control study based on patients and healthy people.SETTING: A neurology department in a university hospital.PARTICIPANTS: Totally 55 patients with stroke hospitalized in Neurology Department of Hospital Affiliated to Medical College of Shaoxing Art and Science College and 35 healthy people taking physical examination from January 2001 to December 2003 in the hospital as controls.METHODS: Patients with acute cerebrovascular disease certified by CT/MR and people in control group took symptoms checklist-90(SCL-90) test within a month after attack.MAIN OUTCOME MEASURES: SCL-90 scores of the two groups.RESULTS: The condition of mental state in patients with acute cerebrovascular disease was worse than that in control group( P ＜ 0.01 ). There existed obvious physical and mental reaction. It was found in subsection analysis that the size and location of the focus had relationship with the degree of mental disorder after attack and it was easier to have mental disorder if the affection lied in left cerebral hemisphere.CONCLUSION: It is necessary to give psychological intervention in prevention and cure of acute cerebrovascular disease. Recognizing bad effect of stroke on psychoactivity may help to guide the treatment.

Objective To explore the role of cyclooxgense-2 (COX-2) in tetrahydrocannabinol (THC)-induced inhibition of long-term depression (LTD) in CA1 area in vitro. Methods The hippocampal slices were prepared at 24 h after intraperitoneal injection of Δ~9-THC (10 mg/kg) or COX-2 inhibitor NS398 (10 mg/kg). Field excitatory post-synaptic potentials (fEPSP) were recorded in the stratum radiatum of the CA1 area in vitro to observe the effect of NS398, a selective inhibitor of COX-2, on the THC-induced inhibition of LTD and THC's effect on membrane excitability in pyramidal neurons by using the whole-cell patch clamp technique. Results ① Low-frequency stimulation (1 Hz, 15 min) induced-LTD in CA1 area was significantly attenuated by Δ9-THC. ② Δ~9-THC did not affect the basal synaptic transmission and membrane excitability (including membrane resting potentials, input resistance and firing property). ③ THC-induced inhibition of LTD was reversed by NS398. ④ THC-induced inhibition of LTD was robustly impaired in COX-2 knockout mice. Conclusion THC-induced inhibition of LTD in CA1 area was mediated via COX-2.

Objective:To observe immunological mechanism of CMXR in the rat free fetal bowel transplant to mouse.Methods:Two groups were established including allogroup and xenogroup.Pathological changes of grafts in each group were examined.Expression of TH type cytokines mRNA and phenotypes of infiltrating cells intragraft were detected by in situ hybridization histochemistry and immunohistochemistry methods respectively.Results:(1)Survival rates of allogroup transplants were higher than those of xenogroup( P

0.05).The results showed that patients treated with Damon3TM brackets pay significantly lower treat- ment time(4.23 months),required significantly lower chairside time(137.15s)than those treated with conventionally-ligated brackets.Conclusions:In matched cases,Damon3TM brackets produced significant reductions in treatment time and the chair- side time,but gained the same treatment results comparing with conventionally-ligated brackets.

Objective To evaluate the role of Src kinase in the induction and maintenance of spinal long-term potentiation(LTP).Methods The C-fiber evoked field potentials were recorded at the superficial layers of spinal dorsal horn at the lumbar enlargement.Results ① Genistein(200?mol/L) or PP2(100?mol/L),a selective Src kinase inhibitor,completely blocked LTP induction when administered at 30min prior to tetanic stimulation.② Genistein or PP2 reversed spinal LTP in a time-dependent manner.At 15min after LTP induction,Genistein(200?mol/L) or PP2(100?mol/L) reversed LTP completely.At 30min after LTP induction,however,the same concentration of Genistein or PP2 did not affect the spinal LTP.Conclusion Activation of Src kinase in spinal dorsal horn may be crucial for the induction and early-phase maintenance of LTP of C-fiber evoked field potentials.

Objective To explore the role of cyclooxgense-2(COX-2) in tetrahydrocannabinol(THC)-induced inhibition of long-term depression(LTD) in CA1 area in vitro.Methods The hippocampal slices were prepared at 24 h after intraperitoneal injection of ?9-THC(10 mg/kg) or COX-2 inhibitor NS398(10 mg/kg).Field excitatory post-synaptic potentials(fEPSP) were recorded in the stratum radiatum of the CA1 area in vitro to observe the effect of NS398,a selective inhibitor of COX-2,on the THC-induced inhibition of LTD and THC's effect on membrane excitability in pyramidal neurons by using the whole-cell patch clamp technique. Results ① Low-frequency stimulation(1 Hz,15 min) induced-LTD in CA1 area was significantly attenuated by ?9-THC.② ?9-THC did not affect the basal synaptic transmission and membrane excitability(including membrane resting potentials,input resistance and firing property).③ THC-induced inhibition of LTD was reversed by NS398.④ THC-induced inhibition of LTD was robustly impaired in COX-2 knockout mice.Conclusion THC-induced inhibition of LTD in CA1 area was mediated via COX-2.

Two thousands six hundreds and forty-four patients of malignant tumors of the breast were diagnosed and treated in our hospital from 1956 to 1990. Twenty-one cases of them were diagnosed mammary sarcoma by pathology in 0. 8% in contcmporary malignant tumors of the breast. The axillary lymph nodes of 6 cases in this group are clinically enlarged in 28. 6%, all of them were proved benign through mieroscopie examination. The age of the patient of mammary sarcoma is younger than that of the patient of mammary careinoma in the group. The patient's age over forty-five years and more than one year course have a hetter prognosis. Mammary sarcoma metastasizes by blood stream or local infiltrate, rarely metastasizes to regional lymph nodes. Mammary sarcoma is treated by removal of the tumor and simple mastectomy. The value of radiotherapy will be futher explored by practice.

TP5 and CsA were injected-intraperitoneally into groups of experimental mice, and NS was used for the contral group. Herpes simplex keratitis models were made subsequently by inoculation of the virus on the cornea. On the 4～6th day after inoculation, slit lamp examination showed that the corneal epithelial and stromal lesions in the TP5 group were significantly more severe than those in the NS group, whereas the group of CsA manifested no such differences. Among the 3 groups of mice, no differences were evident in the formation of corneal neovascularization, which all peaked on the 8th day. The conjuctival and palpebral lesions in the TP5 and CsA groups were also more severe than in NS group. Therefore, immunomodulators should be clinically used with care in accordance with the morbid varieties and conditions.

Pseudomonas aeruginosa was inoculated into corneal stroma of rabbits and the model of keratoconjunc tivitis was established. Empty liposomes were used in a control, group ofloxacin liposome and ofloxacin eyedrop groups as experimental ones. After treatment for six times conjunctival and corneal lesions were examinated. Swabs of conjunctival sac were cultured. Result: in conjunctiva, lesions in the two experimental groups were all significantly less than those in the control group on the 6th, 8th and 11th day. Lesions in the ofloxacin liposomes group were also significantly less than those in the ofloxacin eyedrop group on the6th day. In cornea, lesions in the ofloxacin liposomes group were significantly less than those in the control group on the 6 th, 8th and 11th day. Lesions in the ofloxacin eyedrop group were significantly less than those the control group on the 11th day. In the result of bacterium culture, the culture's positive rate of two experimental gronqs and control group had significant differences on the 6th day. On the 11th day, the positive rate the ofloxacin liposome groups and ofloxacin eyedrop groups increased, and in the ofloxaein liposomes group had a less increase. Conclusion: ofloxacin liposomes and ofloxacin eyedrops were effective in treatments of pseudomonas keratoconjuntivitis in the rabbit model, the effects of ofloxacin liposomes were better than that of ofloxacin eyedrops.