0.05).After reperfusion,Sevo group and Post group resulted in a significant increase(P

Objective To prepare insulin thiolated hyaluronic acid nanoparticles (Ins-HA-Cys-NPs)and study its physicochemical properties. Methods The Ins-HA-Cys-NPs was prepared by ultrasonic emulsifying method,and the properties of nanoparticles including morphology,mean diameter,Zeta potential,entrapment efficiency and drug loading efficiency were studied,as well as the cryoprotectant selection.Results The prepared nanoparticles was round in appearance and the mean diameter was(178.5 ±0.8)nm,the polydispersity index was (0.214 ±0.013)and the Zeta potential was -(38.47 ±0.46 )mV,while the entrapment efficiency was (48.85 ±0.66 )%,drug loading efficiency was (4.79 ±0.13 )%;10%mannitol as cryoprotectant provided uniform and well dispersed suspension of nanoparticles with blue opalescence after redispersion.Conclusion The thiolated hyaluronic acid nanoparticles may be used as the carrier for oral drug delivery system of insulin,and it provides a basis for studies on rats in vivo.

Objective:To establish the quality control methods for Qianliedan particle. Methods:TLC was used to identify Scutel-lariae Barbatae Herba, Astragali Radix, Glycyrrhizae Radix Et, Rhizoma Praeparata Cum Melle, Psoraleae Fructus and Curcumae lon-gae Rhizoma. The content determination item was developed by determining the content of scutellarin in Scutellariae Barbatae Herba by HPLC. Results:The TLC spots were clear and well-separated without any interference from the negative sample. A good linear rela-tionship was established between the peak response and the concentration of scutellarin within the range of 3. 96-79. 20 μg·ml-1(r=0. 999 9). The mean recovery of scutellarin was 99. 34% (RSD=1. 25%, n=9). Conclusion:The established quality control meth-ods are accurate, reliable, stable and specific, which can be well used for the quality control of Qianliedan particle.

Objective To observe the characteristics of multiple evanescent white dot syndrome (MEWDS) with modern multimodal imaging modalities.Methods This was a retrospective case study.Eleven patients (11 eyes) diagnosed with MEWDS were enrolled.There were 10 females and 1 male,mean age was 27.6 years (range 15-41 years).The period between disease onset and visiting to the hospital was between 2 to 13 days,the average time was 4.7 days.All the patients underwent examinations of best corrected visual acuity,slit-lamp biomicroscope,indirect ophthalmoscope,fundus color photography,fundus autofluorescence (FAF),fundus fluorescein angiography (FFA),indocyanine green angiography (ICGA) and spectral domain optical coherence tomography (SD-OCT).The mean follow up duration was 3.2 months.The imaging characteristics were compared.Results Fundus color photography showed foveal orange-red granularity in all eyes.FAF showed strong autofluorescence with a vague boundary.FFA showed a variable number of highly fluorescent fine needle-like dots arranged in a ring in the early stage,and fluorescence remained in the late stage.ICGA showed advanced lesions of vague boundary merged into a large plaque of deep retinal hypofluorescence.SD-OCT showed the hyperreflectant material deposit over the retinal pigment epithelium and extending anteriorly through the interdigitation zone,ellipsoid layer,and toward the external limiting membrane.At the site of extrafoveal lesions,SD-OCT revealed the presence of discontinuities or disruptions centered on the ellipsoid zone to include the interdigitation.Conclusions In MEWDS patients,fundus photography showed foveal orange-red granularity;FFA showed early fluorescent dots distributed in a ring pattern;ICGA showed hypofluorescent lesions in the later stage;SD-OCT showed disruption of the interdigitation zone and ellipsoid zone and accumulations of hyperreflective material that was of variable size and shape;FAF showed strong autofluoresce areas that correlated to spots observed with FFA and ICGA.

Objective To study the clinical effect of orthopedic-orthodontic treatment in crossbite in deciduous dentition through cephalmetric analysis. Methods 12 children with crossbite in deciduous dentition were enrolled in treatment group. Control group included 8 patients with normal deciduous dentition. Modified reverse headgear was used in each patient in the treatment group. Orthodontic force was a-bout 2. 94-4. 90 N (300-500 g) , and the direction of force was under occlusion plane for 37 degree. Each patient wore reverse headgear for 10 hour per day. Orthodontic treatment lasted for 6 months, and then orthodontic treatment began at once. Orthodontic treatment was not over until crossbite was corrected. The cephalmetric change was compared between two groups before treatment (T1) , after orthopedic treatment (T2)and after orthodontic treatment (T3). The effect of orthopedic-orthodontic treatment was analyzed in two group. Results Crossbite was corrected successfully in all patients in the treatment group. After treatment, A point moved forward by 2. 8mm, Is-FHp increased 8. 38 mm, ＜SNA increased 2. 65, incisors in mandible moved backward a little bit. All these changes were statistical different as compared with those in the control group. Conclusion Skeletal and dental deformity are improved progressively after combined orthopedic-orthodontic treatment. It is recommended to perform orthopedic-orthodontic therapy in skeletal crossbite in deciduous dentition.

OBJECTIVE To explore the outcomes of CO2 laser assisted stapedotomy with artificial stapes prostheses in the treatment of advanced otosclerosis. METHODS Between January 2010 and January 2014, 15 patients (16 ears) diagnosed as advanced otosclerosis accepted CO2 laser assisted stapedotomy with artificial stapes implantation in our department. The averaged preoperative air conduction threshold of the speech frequency was 70.21 dB HL, the averaged bone conduction threshold was 38.49 dB HL, the averaged air-bone gap (ABG) was 31.72 dB HL. All cases were followed up for more than 6 months after operation. RESULTS All cases accepted auditory follow up after 6 months postoperatively. The speech frequency average air conduction threshold was 43.7 dB HL, the average bone conduction threshold was 28.95 dB HL, the average ABG was 14.75 dB HL. The ABG≤20 dB was achieved in 9 ears (56.3%) and ABG closure (≤10 dB) was achieved in 6 ears (37.5%). No cases appeared intractable vertigo, sensorineural hearing loss, secondary facial paralysis and other serious complications. CONCLUSION CO2 laser assisted technique reduced the probability of serious complications of stapedotomy, most patients with hearing level improved significantly. It's a safe, practical, relatively economical choice for advanced otosclerosis.

Objective To investigate needs of caregivers of patients with schizophrenia for mental health knowledge and provide the scientific evidence for formulating the general intervention to improve the caregivers′mental health knowledge and skills . Methods Sixty-five caregivers of the patients with schizophrenia participated in the survey by a general state questionnaire and the requirement to mental health knowledge and skill questionnaire using the convenience sampling method. The survey included mental illness knowledge drug, knowledge rehabilitative therapy and self-adjustment. Results Among all the items of the questionnaire, the 3 items they needed most was mental illness knowledge, the preventing method and the symptoms of the recurrent diseases. The 3 items they needed most in respect of drug knowledge were what examinations are needed when taking special drugs, the adverse reactions of the antipsychotic drugs and the importance of persisting in taking drugs. The 3 they needed most in respect of rehabilitative therapy were the relationship between psychology and disease rehabilitation, the role of mental treatment and the role of work-entertainment therapy. The 3 items they needed most in respect of self-adjustment included sleep adjustment, self-adjustment of affects and self-management of daily life and instructions on dieting. Conclusions The caregivers′mental health knowledge and skills are not enough. Therefore, their mental health knowledge and skills should be strengthened so that they can take better care of the patients to realize comprehensive rehabilitation of patients.

OBJECTIVE:To discuss the choice of artificial thigh bone prosthetic material and curative effects of artificial thigh bone prosthesis for treating femur intertrochanteric comminuted fracture of senior patients.METHODS:We retrieved China National Knowledge Infrastructure (CNKI) in Chinese (1977/2009) and Medline database in English (1977/2009) with the key words of "femur intertrochanteric fracture;artificial femoral head arthroplasty;elderly patient".Totally 83 articles were retrieved,and 34 articles were included in accordance with inclusion and exclusion criteria.Literatures addressing artificial thigh bone prosthesis,intertrochanteric fracture in elderly patients and artificial femoral head replacement in treatment of femur intertrochanteric fracture were summarized,and curative effect of artificial thigh bone prosthesis for treating intertrochanteric fracture in elderly patients was explored.RESULTS:Artificial thigh bone prosthesis had been widely used in treatment of various femur intertrochantedc fractures.There are many types of artificial thigh bone prosthesis such as metal material,high polymer polyethylene,ceramic material and carbon material,which had obtained significant outcomes.Function of artificial thigh bone prosthesis was improved gradually,and became widely in clinic.Artificial thigh bone prosthesis has presently been an optimal method for treating femur intertrochanteric comminuted fracture in elderly patients.However,this method exist many problems that require to be solved such as high operation risk and many complications.CONCLUSION:There are many materials for choosing artificial thigh bone prosthesis,with various advantages and disadvantages.Presently,complex artificial thigh bone prosthesis is commonly used in clinic.Artificial thigh bone prosthesis is an ideal method to treat femur intertrochanteric comminuted fracture of elderly patients.

Objective To investigate the frequent species of pathogenic bacteria causing infections in burn patients and their re‐sistance to commonly used antibacterial agents ,so as to provide references for rational use of antibacterials in clinic .Methods The distribution and drug susceptibility of pathogenic bacteria isolated from secretions of wound surfaces of 140 cases of burn patients from January 2012 to December 2014 were retrospectively analyzed .Results A total of 152 strains of pathogenic bacteria were iso‐lated .The gram‐negative bacteria accounted for 59 .2% ,in which Pseudomonas aeruginosa ,Proteus mirabilis and Acinetobacter bau‐mannii were the most common isolates ;the gram‐positive bacteria accounted for 34 .2% ,in which Staphylococcus aures ,Staphylo‐coccus haemolyticus and Enterococcus faecalis were the most common isolates ;and fungi were accounted for 6 .6% .A majority of these isolates were multiple resistant to the antibacterial agents .Conclusion Culturing ,identifing and carring out drug‐sensitivity test of pathogenic bacteria isolated from burn patients could provide basis for rational application of antibacterial agents and effec‐tive control of infection .

Objective To establish the quality control methods for Qihuang Fuzheng particle .Methods Astragali Ra-dix ,Rehmanniae Radix Praeparata ,Corni Fructus ,Chuanxiong Rhizoma ,Angelicae Sinensis Radix , Paeoniae Radix Rubra ,Moutan Cortex ,and Ophiopogonis Radix were identified with TLC .HPLC method was used for loganin assay in Cor-ni Fructus .Results TLC identification methods for the main components were established .The TLC spots were clear with good separation .No interference was detected from the negative samples .The peak response and concentration of loganin showed good linear relationship over the range of 4 .02-80 .40 μg/ml (r=0 .9999) .The mean recovery of loganin was 99 .02％(RSD=0 .64％ ,n=9) .Conclusion The established quality control methods are accurate ,reliable ,and specific ,which lay a foundation for the quality control of Qihuang Fuzheng particle .