Objectives To culture and isolate Malassezia furfur which were suspected in sputum smears,and then to identify the isolates systematically.Methods From March 2010 to September 2011,133 lower respiratory tract secretion samples were collected in Beijing Hospital of the Ministry of Health which were suspected containing Malassezia furfur by Parker ink staining.The samples were inoculated and cultured on CHROMagar mediums containing 1％ Tween 60 ( which was modified from Candida chromogenic media) in the atmospheric environment at 35 ℃.The suspected colonies were selected and cultured on both SDA plates containing 0.5％ Tween 40 and SDA plates containing 0.5％ Tween 60.The traditional phenotypic identification methods were used to identify the pure colonies,including staining,growth on Sabouraud agar plates,Tween test,decomposing Escalin,Catalase test,Tween precipitation test and castor oil test.Then the suspected colonies were identified by analyzing the sequences of 18s rRNAs.Results The isolation rate of Malasseziafurfur from lower respiratory tract secretions was 47.4％ (63/133) and 63 strains were also identified as Malassezia furfur by traditional phenotypic identification methods combined with the 18s rRNA gene sequence analysis.Malassezia can be significantly distinguished from Candida by direct smear of the specimen with Parker ink staining.Conclusions Malassezia furfur was found as pink colonies on modified Candida chromogenic media which were significantly different from other Candida colonies.The isolation rate of lower respiratory tract secretions can be improved by modified Candida chromogenic media.The identification accuracy can be further improved by combining traditional phenotypic identification methods with 18s rRNA sequence analysis.

Objective:To analyze the clinical features of oral emergency patients in general health, and to guide the oral emergency patients in the treatment, and prevention of accidents.Methods:The retro-spective study was performed on the day visits to the emergency department in Peking University School and Hospital of Stomatology from February to November 2013.The basic information of the newly diag-nosed adult, their overall health status, American society of anesthesiologists(ASA) classification and oral diagnosis information were analyzed and summarized.Results:The cardiovascular and cerebrovascular diseases(6.4%), hypertension(13.4%), endocrine system diseases(4.2%)were most common in the patients.With the increase of age, the associated systemic diseases had increased in number and in ASA classification.Periapical disease ( 24.6%) , dental pulp disease ( 24.4%) , and periodontal disease (18.0%) accounted for about 2/3.Conclusion:The general health status of patients with oral emergen-cy worsened with the increase of age.Oral surgeons should make risk assessment before treatment.ASA classification system can help doctors predict the patient risk, take corresponding measures in advance, and help to reduce the occurrence of accidents.

Objective We aimed to assess the influence of space teaching on junior-college nursing students' autonomous learning ability.Methods Using random number table,two classes of junior-college nursing students of Grade 2012 were selected and divided into the experimental group (46 students) and the control group (47 students) according to their classes.Students in the control group received routine multimedia teaching.Students in the experimental group received space teaching based on the platform of World University City.The effect of space teaching on developing nursing students' autonomous learning ability was assessed with the help of their test results and the nursing students' autonomous learning ability evaluation form.Results After carrying out space teaching,the average grade,theoretical performance,total points,the total score of autonomous learning ability and the score of each dimension in the experimental group and the differences were remarkably higher than those of the control group (t value was 2.80,2.45,2.29 and 11.58).Conclusions Space teaching can assist in arousing nursing students' learning motivation and improving nursing students' independent learning ability.It can be used for reference and promoted.

Objective　 To investigate the relationship between of serum bilirubin concentrations and risk of coronary heart drsease (CHD). Methods　 Serum bilirubin concentrations were determined in 12 cases with acute myocardial infarction(AMI)、37 cases with unstable angina pectoris (UAP) and 30 cases with UAP with arrhythmia. Results　 The serum total bilirubin(TB)、direct bilirubin(DB)、indirect bilirubin(IB) concentrations were lower in the three study groups of CHD than in the controls. There was significant difference between the patients with CHD and the controls. However, there was no significant difference among the three study groups of CHD. Conclusion　 Serum bilirubin concentrations was inversely and statistically significantly related to risk of CHD.

AIM: To study the expression and change of growth hormone receptors in experimental cirrhotic liver in rats. METHODS: Thioacetamide-induced liver cirrhosis was developed in Sprague-Dawley rats. The expression of growth hormone receptor and its mRNA was investigated by radio-ligand binding assay, revere transcript-polymerase chain reaction (RT-PCR) and digital image analysis in liver tissue or hepatocytes taken from normal control or cirrhotic rats with different stages of cirrhosis. RESULTS: Growth hormone-specific singular binding sties, namely growth hormone receptors, were detected in liver tissue or hepatocytes from rat samples with cirrhosis. The binding capacity of growth hormone receptors in rat cirrhotic live tissue was significant lower than that in normal control, and it decreased gradually along with the accumulation of collagen in the process of formation and development of liver cirrhosis. The binding sites of growth hormone receptors in rat cirrhotic liver cells were significantly lower than that in normal control. The expression of growth hormone receptor mRNA in rat cirrhotic liver tissue was also significant lower than that in normal control. CONCLUSIONS: The growth hormone receptor was expressed in cirrhotic livers at a reduced level in isolated hepatocytes or live tissue of cirrhotic rats, and lesser expression of growth hormone receptors was found in later stage of cirrhosis. The suppressed expression of growth hormone receptor was partly due to the reduced expression of its mRNA in cirrhotic liver tissue.

Objective To study the roles of hepatocyte cytochrome P450 2E1 in model of nonalcoholic steatosis in rats Methods A total of 40 Wistar rats were randomly divided into two groups: control group (C) and high fat diet induced fatty liver group (H) The expression of hepatocyte cytochrome P450 2E1 antigen in rat model of nonalcoholic steatosis was detected by immunohistochemical method and Western blotting Malondialdehyde (MDA) contents were also determined Results MDA contents and the expression of hepatocyte cytochrome P450 2E1 antigen in rat model of nonalcoholic steatosis induced by high fat diet were higher than those in the normal controls ( P

In order to investigate the effects of verapamil on the proliferation of meningiomas cells in vitro and in vivo, the cultured meningiomas cells were cultured with verapamil at different concentrations for 24 h and the inhibitory effects of verapamil on cell proliferation were observed by MTT method. The meningiomas model was established by implanting the newly removed tumor fragments into the nude mice subcutaneously. The nude mice with tumors were divided into two groups: verapamil-treated group and control group. Tumor volumes were measured and after 12 weeks the tumors were taken out and examined histologically. The expression of proliferating cell nuclear antigen (PCNA) in the tumors was detected by using immunohistochemistry. It was found that verapamil could inhibit the growth of cultured meningiomas cells in a concentration-dependant manner. The inhibitory effect could be observed in the concentration of 1 micromol/L verapamil and the most obvious effects appeared in the concentration of 100 micromol/L. Tumor volume in the verapamiltreated group was obviously smaller than that in the control group (211.40+/-5.50 vs 163.94+/-3.62, P<0.01) and the expression of PCNA was also lower (1.52+/-0.24 vs 2.86+/-0.53, P<0.05). Tumor inhibition rate was about 22.45%. It was suggested that verapamil could inhibit the proliferation and growth of meningiomas cells in vitro and in vivo.

Objective To analyze the relationship between the survival and expressions of EGFR and Her-2, and explore the prognostic significance of EGFR and Her-2 in gastric cancer patient by detecting the expressions of EGFR and Her-2 in gastric cancer specimens. Methods EGFR and Her-2 were detected by immunohistochemistry. The relation between their expressions with their clinical characteristics and prognosis were analyzed. Results The over-expression rates of EGFR and Her-2 are 27.4 % and 32.1% respectively in 84 cases. The over-expression rates of EGFR and Her-2 in patients with Ⅲ + Ⅳ stages were significantly higher than those with Ⅰ + Ⅱ stages(respectively, P =0.014 and P =0.005). The positive rate in patients with T3+T4 stages were significantly higher than those with T1+T2 stages (P=0.033); and the positive rate in patients with positive lymph node was higher significantly than that with negative (P =0.033). The OS and PFS in over-expression patients were significantly longer than low-expression. Conclusion The overexpression of EGFR and Her-2 could be used to evaluate the degree of differentiation and prognosis of patients with gastric cancer.

Objective To investigate the action of integron-mediated multidrug resistance in clinical multi-resistant Pseudomonas aeruginosa.Methods The strains of multi-resistant Pseudomonas aeruginosa were selected with K-B susceptibility method. The three-dimensional method was taken to differentiate the various beta-lactamases. The integron was determined by PCR with integron-specific primer. DNA sequencing was also used to analyze resistance-related gene in the integron in multi-resistant Pseudomonas aeruginosa.Results The positive rate of integron detection in multi-resistant Pseudomonas aeruginosa was 42.4%.Three kinds of different length integron were found .They were 750bp,1 000bp and 2 500bp and aadB,aadA2,aadB,aac6-II and PSE-1 were the resistance-related gene cassette in the integrons.Conclusions Integron took part in mutiple resistance in multi-resistant Pseudomonas aeruginosa.We should pay more attention to integron-mediated horizontal transference of mulidurg resistance to lessen the occurrence of multi-resistant strains.

Objective To discuss a new method of BMSCs culture which can reduce the lead-in of extraneous antigenic substances in the process of cell culture, and can thus better meet the demand on the quantity and biological characteristics of BMSCs in the clinical use of tissue engineering bone. Methods BMSCs from a human adult in vitro were induced by using autologous platelet-rich plasma instead of animal serum in high-carbohydrate DMEM (Dulbecco's minimum essential medium). After the cultured BMSCs were amplified rapidly by Rotary Cell Culture System, their growth status was observed and their biological characteristics were detected. Results The cells were in good growth status and presented fine biological characteristics. Their growth speed in Rotary Cell Culture System was observed to be evidently faster than that by conventional methods. Conclusions Rotary Cell Culture System by autologous platelet-rich plasma instead of animal serum is a good culture method to induce human BMSCs in vitro. The quantity and biological characteristics of BMSCs cultured in this way can meet the demand in clinical use.