Objective To promote local swelling, reduce muscle atrophy and to prevent the knee joint and knee devices adhesion, thus maximizing recovery in patients with a heavy load and walking function. Methods From 6 hours to 6 or 8 weeks after the operation, patients did knee rehabilitation exercises. Results 35 cases of distal femoral fracture patients did knee rehabilitation exercises. The incision healed well. The scope of activities of the knee was in normal range after an average of three to four months. Flexion were over 90 ? without pain and deformity. Conclusion Patients should do rehabilitation exercises as early as possible. Scientific and orderly rehabilitation care promotes the wound and fracture healing.

OBJECTIVE To investigate the antibiotics resistance and the presence of resistant gene of Staphylococcus aureus isolated from nosocomial infection patients in our hospital.METHODS Antibiotic susceptibility tests were conducted by Microscan WalkAway-40,the antibiotics resistant genes of aac(6')/aph(2″),aph(3')-Ⅲ,ant(4',4″),TEM,tetM,macA,erm and qacA/B of 16 MRSA and 24 MSSA strains were detected by PCR.RESULTS The percentage of MRSA in this investigation was 59.4%,the positive rate of ?-lactamases of 192 S.aureus strains was 98.9%,resistant rate of S.aureus to ?-lactam antibiotics was 52.1-99.0%,and to erythromycin,clindamycin,clarithromycin,ciprofloxacin,norfloxacin,gentamicin and tetracycline was 52.1%,78.1%,85.5%,56.3%,61.1%,55.2%,and 69.8%,respectively,but to nitrofurantoin and sulfamethoxazole/trimethoprim were relatively lower(1.1% and 10.5%) than the others.VISA,h-VISA and VRSA were not measured.To the most of antibiotics,the resistant rate of MRSA was higher than MSSA.The presence of resistant gene of MRSA and MSSA was different.There was more positive percentage of gene in MRSA than in MSSA.CONCLUSIONS Antibiotics resistance of S.aureus isolated from nosocomial infection patients in our hospital is severe.Compared to MSSA,MRSA has higher antibiotic resistant rate and more complicated drug-resistant mechanism.Some measures should be done to reduce the antibiotic resistance of S.aureus.

Objective To explore the clinical efficacy of lnstaTrak3500 Plus with Fluoro Trak magnetic imaging guidance for spine surgery, Methods The construction and operation progress of the system were applied in thoracic-lumbar spine surgery, Results The electric-magnetic guided imaging system can improve success rate in thoracic-lumbar spine surgery,and all operative pedicle screw were inserted accurately with no related complications error. Conclusion The electric-magnetic imaging system can improve the accuracy of operations and guide mini-invasive surgery, which can significantly decrease X-ray irradiation dosage.

Objective:To investigate the effects of periploca forrestii schltr in the treatment of acute gout arthritis.Methods:60 healthy male SD rats were equally randomly divided into 6 groups:normal control group( NC) ,model group( M group) ,colchicine group (C group),high doses group of periploca forrestii schltr(HD group),middle doses group of periploca forrestii schltr(MD group) and low doses group of periploca forrestii schltr( LD group).Except the normal control group,model of gouty arthritis was induced in other groups by uric acid salt,colchicine(positive control) and different dose of periploca forrestii schltr were given by intragastric ad minis-tration.Swelling dimension of joints were observed at 3,5,7 days after treatment.All rats were killed after 7 days of treatment and ankle joint tissue was taken for pathological examination and the peripheral blood of rats was prepared for detecting the expression of interleukin 1β(IL-1β),IL-6,IL-8 and tumor necrosis factor(TNF-α) using enzyme linked immunosorbent test(ELISA).Results:The ankle joint swelling of periploca forrestii schltr group was significantly lower than that in the model group,and the effect of high doses group was much better than the low doses group after 7 days treatment(P<0.05);compared with model group,the inflammatory cells of each treatment groups were decreased and high doses group did not differ from that of normal control group;the levels of IL-1β,IL-6,IL-8 and TNF-αin periploca forrestii schltr group were dramatically lower than those in the model group in a dose-dependent manner.Conclusion:Periploca forrestii schltr has good therapeutic effect in rats with acute gouty arthritis and shows a dose-dependent response,and the mechanism may relate to the inhibition of inflammatory cytokines expression.

Objective To investigate the immunomodulatory effects of Tetrastigma hypoglaucum Planch in the treatment of rheumatoid arthritis. Methods Sixty SD rats were ramdomly divided into normal control group, model control group,low-,middle-and high-dose Tetrastigma hypoglaucum Planch groups (50,100 and 200 mg.kg-1,respectively),and tripterygium glycosides tablet group. Except for normal control group,rheumatoid arthritis model was established by using bovine typeⅡcollagen in SD rats of the other groups. Organ index,plasma levels of IL-1β,IL-6,TNF-αand anti-CⅡin rat rheumatoid arthritis models were evaluated. Results The kidney index of the low-dose/middle-dose Tetrastigma hypoglaucum Planch groups were significantly different from that of the model control group (P<0.05). Significant difference was also found in the spleen index between the high-dose Tetrastigma hypoglaucum Planch group and the normal control group ( P<0.05) . The plasma levels of IL-1β,IL-6,TNF-αand anti-CⅡantibody were significantly higher in the model control group than in the normal control group. Meanwhile, the plasma IL-1β, IL-6 and TNF-α levels of the middle-dose/high-dose Tetrastigma hypoglaucum Planch groups were significantly lower than those of the model group ( P<0.01) . The levels of anti-CII antibody were significantly lower in the low-,middle-and high-dose Tetrastigma hypoglaucum Planch groups than in the model control group. Conclusion It is worthwhile to explore and develop Tetrastigma hypoglaucum Planch since it possesses immunomodulatory effects and may be applied in the treatment of rheumatoid arthritis.

Objective:To study the genotyping characteristics of Brucella strains isolated from Lianyungang City (non-brucellosis epidemic area) of Jiangsu Province. Methods:Preliminary identification of 13 suspected strains of Brucella isolated from blood culture in Clinical Microbiology Laboratory of the First People's Hospital of Lianyungang City in 2018 was conducted; at the same time, the specific gene bcsp31 and insertion sequence IS-711 of Brucella were detected by quantitative real-time PCR (Real-time PCR), and the identification results were rechecked and typed. Multiple locus variable-number tandem repeat analysis (MLVA) was applied for genotyping, and the sequencing results were edited by Mega 4.0 software. Results:All the 13 strains were identified as Brucella by preliminary identification. Real-time PCR confirmed that all the 13 strains were Brucella melitensis. The results of MLVA showed that 13 strains of Brucella melitensis were divided into 12 genotypes and clustered in the "middle Mediterranean cluster". Among 13 strains of Brucella melitensis, 3 strains were biovar 1, 2 strains were biovar 2 and 8 strains were biovar 3. Conclusion:All the Brucella strains isolated from Lianyungang City are Brucella melitensis and the MLVA cluster is in the "middle Mediterranean cluster".