Objective To investigate the protective effects of low-frequency pulsed electromagnetic field (LFPMF) on neuron following cerebral ischemia-reperfusion injury in rats.Methods 24 adult male Sprague-Dawley rats were randomly divided into irradiation group (n=12) and control group (n=12). The global cerebral ischemia-reperfusion models of rats were produced by modified Pulsineli method. Rats in irradiation group were exposed to 20 mT intensity LFPMF of 10 Hz for 45 minutes per day for 4 days. The electroencephalogram (EEG) was recorded by powerlab system. Nissl staining was used to perform quantitative analysis of neuron number and immunohistochemistry staining provided the information of the distribution of positive cells to caspase-3.Results Compared with the control group, the amplitude of EEG was significantly higher in irradiation group [(10.27?1.12)?V versus (8.95?1.04)?V] (P

Objective To study the calculational method for the radiotherapy facilities of the medical linear accelerator' s useful beam towars its maze.Methods The shielding calculation was made under the relevant national standards for a radiotherapy treatment room and compared with the test results.Results The dose rates at the maze entrance as calculated and measured were 89 and 86 μSv/h inside the maze door,as well as 5.7 and 6.2 μSv/h outside the maze door,respectively.The calculated results were consistent with measured results.Conclusions By comparison of calculated results with measured results,the accuracy of the theoretical calculation method could be verified.

Objective To establish pulmonary acute lung injury(ALI)model in rats.Methods ALI model was induced in rats by intratracheal Escherichia coli injection[3 ml/kg,O111B4,(4.0～6.0)×1012 CFU/L].Mechanical ventilation was applied 12,24,36,48 and 72 h after Escherichia coli injection,PaO_2/FiO_2 and dynamic compliance were recorded,and the normal control group was also subjected to mechanical ventilation.After the experiment,lungs were fixed with formalin to perform pathological examination.Results At 12,24,36,48 and 72 h,the PaO_2/FiO_2 were(30.71±7.95)kPa,(21.66±5.34)kPa,(21.09±4.75)kPa,(25.01±8.78)kPa and(33.82±8.02)kPa,respectively,which were significantly lower than that in the normal control group(63.82±3.03)kPa(P＜0.01).At 12,24,36,48 and 72 h,the Cdyn were(4.23±0.13)ml/(kg·kPa),(4.19±0.96)ml/(kg·kPa),(4.28±0.69)ml/(kg·kPa),(4.44±0.62)ml/(kg·kPa)and(4.58±0.35)ml/(kg·kPa)respectively,which were significantly lower than that in the normal control group(8.16±0.78)mL/(kg·kPa)(P＜0.01).At 12,24,36,48 and 72,the percentage of ALI was 71.4%,100.0%,100.0%,83.3%and 57.1%respectively,and the percentage of ARDS was 28.6%,85.7%,83.3%,66.7%,14.3%respectively.As for pathological examinations,predominance of alveolar collapse,fibrinous exudates,alveolar wall edema and neutrophil recruitment into the alveolar space was observed.Hyaline membrane formation was found.At 72 h,inflammation was relieved.Conclusion We successfully established pulmonary ALI/ARDS model in rats induced by intratracheal Escherichia coli injection,and acquired some useful information by observing the lung function and morphological changes at different time points.

Objective To explore the anti-inflammatory effect of antiflammin-2 (AF2) and recombinant peptide sequence 2(R2) on acute lung injury of mouse. To observe the expression of clara cell 16000 protein (CC16) and surfactant protein A (SP-A) in the lung of mouse inoculated with lipopolysaccharide (LPS) and the impact of AF2,R2,and glucocorticoids(hydrocortisone,HC) may have on the expression of the CC16 and SP-A in the lung of mice with acute lung injury. Methods Balb/c mice were inoculated with LPS (5 mg/kg) by intraperitoneal injection to set up ALI mice model. Mice weighed from 15 g to 16 g were grouped into control group, model group and treated groups respectively treated with AF2, R2 or HC. Mice in the control group were injected with physiological saline solution, while mice in the other four groups were inoculated with LPS to induce acute lung injury. Then animals in the treated groups were treated with AF2, R2 or HC each on a dose of 2 mg/kg also through intraperitoneal injection,while those of the control group and the model group, were given equivalent physiological saline solution as a placebo. The respiratory rate of all of these animals were recorded 6 hours after the injection. And at the time point of 12 hour,all the mice were sacrificed for a preparation of the whole lung tissue for the sake of a pathological investigation ,or for extractions of RNA for a semiquantitative analysis of the expression of CC16 and SP-A within the lungs. Results (1) An obvious attenuation of the respiratory rates of the three treated groups were observed when comparing with that of the mice in the model group without any anti-inflammatory treatment. (2) Remarkable extenuation of the extent of intra-alveolar and intersticial hemorrhage and infiltration of inflammatory cells were observed within the treated groups comparing with that of the model group. (3) An attenuate expressions of CC16 or SP-A were observed in the model group,while obvious uptrend of CC16 expression was observed in AF2 treated groups and increase of SP-A expressions were found in R2 and HC treated groups. Conclusion The anti-inflammatory effect of the peptide, AF-2 or R2, has been conformed on ALI mice model induced by LPS.

PURPOSE: The aim of this study was to analyze an injury scene during fly fish boat riding (FBR).METHODS: We conducted survey on 12 patients who had humerus shaft fractures during FBR between 2011 and 2016 at three university-based emergency rooms. Individual information, injury mechanism, classification of humerus shaft fracture, and combined injury were recorded from medical document and telephone interview.RESULTS: The injury happened when the kite was turned over and fall into the water in 10 patients (82%); the kite was turned over in the air in one patient (9%), and a leash between kite and boat was broken in one patient (9%). All patients showed 12-B1 or 12-B3 type distal humerus shaft fracture. And there were combined contralateral distal humeral shaft fractures in two patients, vertebral compression fracture in one patient, and radial nerve injury in four patients.CONCLUSION: Riding position and injury mechanism such as turning over may affect distal humerus shaft fractures with butterfly fragment during FBR.
Butterflies ; Classification ; Diptera ; Emergency Service, Hospital ; Fractures, Compression ; Humans ; Humeral Fractures ; Humerus ; Interviews as Topic ; Radial Nerve ; Ships ; Water

Clinical and imaging data of 11 patients with dysembryoplastic neuroepithelial tumors (DNET) and 15 patients with low-grade glioma (LGG) admitted in Northern Jiangsu People's Hospital were analyzed retrospectively.Routine MRI scan,diffusion weighted imaging (DWI) and enhanced scan were performed.The workstation automatically generated apparent diffusion coefficient (ADC) maps and then to obtain ADC values of the tumor parenchymal area and the contralateral normal reference area.Relative tumor/reference ADC values (rADC) were also calculated.The ADC values of parenchymal regions of tumor and contralateral normal reference areas and the rADC between DNET and LGG were compared.There was significant difference in age distribution between the two groups [(16.6± 13.1) vs.(43.0± 19.2) years,t=3.938,P＜0.01].Six out of 11 DNET cases and none of 15 LGG cases were cuneiform or fan-shaped (P＜0.01);5/11 DNET and 0/15 LGG showed circular high signal in fluid attenuated inversion recovery-T2 weighted imaging (T2FLAIR) sequence (P＜0.01),while there no significant differences in intracapsular segmentation,peritumor edema and mass effect,enhancement,and skull compression between two groups (all P＞0.05).The ADC values of tumor parenchymal regions in both groups were significantly higher than those in contralateral reference regions (both P＜0.01),the rADC of DNET was significantly higher than that of LGG (P＜0.01).It is difficult to identify DNET and LGG by conventional image morphology,however the rADC value of DNET in DWI is significantly higher than that of LGG,and can provide important reference for differential diagnosis between them.

Objective To investigate the expression of Sirtuin1 (SIRT1) in brain tissue of rats with different doses of fluorosis-induced brain injury.Methods Forty-eight clean SD rats were divided into 4 groups based on body weight (90-100 g) via the random number table method:the control group (normal feed containing fluoride 4.5 mg/kg),low fluoride groups (feed containing fluoride 25.0 mg/kg),middle fluoride groups (feed containing fluoride 50.0 mg/kg),high fluoride groups (feed containing fluoride 100.0 mg&g),twelve rats in each group,half male and half female.Rats in each group drank tap water freely.Low,middle and high fluorine groups were free to eat the designated different formulations of raw coal and mixed peat baked corn feed,other feed ingredients were the same as those in control group,the rats were sacrificed at 90 d of fluorine exposure.At 7 d before the rats were sacrificed,Morris water maze and platform experiment were employed to test the ability of learning and memory in rats.Take the brain tissue and thigh long bones after the rats were sacrificed,immediately.The fluorine ion selective electrode method was used to detect urinary fluoride and fluorine content in bone in rats.The brain SIRT1 mRNA and protein expression levels were detected by Real-Time PCR and Western blotting,respectively.Results In the space exploration experiments,the mean time to first passage of platform of the rats in control group and low,middle and high fluorine groups were (9.8 ± 3.5),(15.8 ± 5.1),(22.2 ± 7.9) and (30.5 ± 8.5) s,respectively.The differences between groups were statistically significant (F =10.853,P ＜ 0.05).The number of passed through the platform of the rats in control and low,middle and high fluorine groups were (5.2 ± 2.1),(3.3 ± 1.6),(1.3 ± 1.1) and (1.2 ± 0.8) time/60 s,respectively.The differences between groups were statistically significant (F =10.105,P＜ 0.05).In the control group and low,middle and high fluoride groups,the resting time of the original platform quadrant were (30.5 ± 9.8),(22.7 ± 4.6),(13.8 ± 4.8) and (7.0 ± 2.4) s,respectively.The differences between groups were statistically significant (F =17.433,P ＜ 0.05).And the middle,high fluoride groups compared with the control group was significantly different (P ＜ 0.05).With increase of fluoride dosage,the first time to cross the platform gradually extended,the number of crossing the platform and the original platform quadrant dwell time decreased gradually,the differences between the middle,high fluoride groups and the low fluoride group were statistically significant (P ＜ 0.05).The mRNA expression of SIRT1 in control group and low,middle and high fluoride groups were 0.979 ± 0.088,0.907 ± 0.050,0.426 ± 0.073,0.219 ± 0.092,respectively.The differences between groups were statistically significant (F =136.837,P ＜ 0.05).The levels of SIRT1 protein in control group,low,middle and high fluoride groups were 1.224 ± 0.139,0.988 ± 0.096,0.581 ± 0.084 and 0.269 ± 0.066,respectively.The differences between groups were statistically significant (F =107.961,P ＜ 0.05).The levels of SIRT1 mRNA and protein were gradually decreased with increase of fluoride dose in the low,middle and high fluoride groups (P ＜ 0.05).Conclusions Fluorosis can affect the learning and memory ability of rats.SIRT1 mRNA and protein expressed in rat brain is decreased,which is more obvious with the increase of fluoride dose.

Infectious bone defect is bone defect with infection or as a result of treatment of bone infection. It requires surgical intervention, and the treatment processes are complex and long, which include bone infection control,bone defect repair and even complex soft tissue reconstructions in some cases. Failure to achieve the goals in any step may lead to the failure of the overall treatment. Therefore, infectious bone defect has been a worldwide challenge in the field of orthopedics. Conventionally, sequestrectomy, bone grafting, bone transport, and systemic/local antibiotic treatment are standard therapies. Radical debridement remains one of the cornerstones for the management of bone infection. However, the scale of debridement and the timing and method of bone defect reconstruction remain controversial. With the clinical application of induced membrane technique, effective infection control and rapid bone reconstruction have been achieved in the management of infectious bone defect. The induced membrane technique has attracted more interests and attention, but the lack of understanding the basic principles of infection control and technical details may hamper the clinical outcomes of induced membrane technique and complications can possibly occur. Therefore, the Chinese Orthopedic Association organized domestic orthopedic experts to formulate An evidence-based clinical guideline for the treatment of infectious bone defect with induced membrane technique ( version 2023) according to the evidence-based method and put forward recommendations on infectious bone defect from the aspects of precise diagnosis, preoperative evaluation, operation procedure, postoperative management and rehabilitation, so as to provide useful references for the treatment of infectious bone defect with induced membrane technique.