MLN4924 can inhibit the proliferation,invasion and metastasis of tumor by inducing tumor cells apoptosis,senescence and autophagy,which can inhibit tumor angiogenesis and enhance the sensitivity of radiotherapy and chemotherapy.Therefore,MLN4924 plays a good anti-tumor effect.

IFIT family genes are a kind of interferon stimulated genes (ISGs), and play important roles in antiviral sector and immunity regulation. To study the regulatory effect of IFIT family genes during influenza A virus (IAV) infection, we used RNA-sequencing analysis (RNA-Seq) technique and found that when 293T cells were infected by A/WSN/33 (WSN), the concentration of IFIT family genes were increased. Further study reveals that overexpression of IFIT2 or IFIT3 could inhibit IAV replication and transcription, and cause the dose-dependent inhibition of polymerase activity of vRNP. In addition, IFIT2 and IFIT3 encoding protein could colocalize with NS1 in 293T cells infected by WSN, indicating that they might interact with each other. The results suggest that IFIT family genes can inhibit the replication and transcription of IAV, which contributes to our understanding of the regulatory effect of host factors during influenza virus infection.
HEK293 Cells ; Humans ; Influenza A virus ; physiology ; Influenza, Human ; genetics ; Intracellular Signaling Peptides and Proteins ; genetics ; Proteins ; genetics ; Virus Replication

Objective To investigate effect of modified yinqiao powdeRon the expression of gene profiling in monkey with hand-foot-mouth disease infected by CA16 cells of genomics.Methods Thirty SPF Ganges riveRyoung monkey were randomly divided into treatment group (n=15) and control group (n=15).The monkeys in treatment group were treated with yinqiao powder,while the monkeys in control group were treated nothing.AfteR7 days,sample gene expression profiles were analyzed by PCA.The gene expression patterns between two groups were studied.Results Totally 894 differentially expressed genes were screened by gene expression profiles,which were significantly clustered into 2 majoRtypes.Differential expression of genes involved in the enrichment of moleculaRfunction,including many inflammatory chemokines expression,cell communication,cell cycle,regulation of the immune system process,transcription and metabolism process of biological process.Conclusion There are significant differences in the gene expression profile of ovarian granulosa cells between the two groups.The biological processes involved in the differential expression of genes are related to the effects of yinqiao powdeRon CA16 induced inflammatory chemotaxis and immune system response.

Objective To investigate effect of modified yinqiao powdeRon the expression of gene profiling in monkey with hand-foot-mouth disease infected by CA16 cells of genomics.Methods Thirty SPF Ganges riveRyoung monkey were randomly divided into treatment group (n=15) and control group (n=15).The monkeys in treatment group were treated with yinqiao powder,while the monkeys in control group were treated nothing.AfteR7 days,sample gene expression profiles were analyzed by PCA.The gene expression patterns between two groups were studied.Results Totally 894 differentially expressed genes were screened by gene expression profiles,which were significantly clustered into 2 majoRtypes.Differential expression of genes involved in the enrichment of moleculaRfunction,including many inflammatory chemokines expression,cell communication,cell cycle,regulation of the immune system process,transcription and metabolism process of biological process.Conclusion There are significant differences in the gene expression profile of ovarian granulosa cells between the two groups.The biological processes involved in the differential expression of genes are related to the effects of yinqiao powdeRon CA16 induced inflammatory chemotaxis and immune system response.

Objective:To report a pediatric organ donor with brain death due to wild mushroom poisoning to examine whether or not brain death caused by mushroom poisoning might become a potential organ donor and how to evaluate donated organs.Methods:Strict clinical observations, laboratory tests and biopsy were performed for potential donor.Results:This donor's clinical changes were consistent with toxic hepatitis. Gross morphology, laboratory examinations and pathological biopsy of two kidneys were generally normal during organ acquisition. Two kidneys were assigned to two adult recipients and liver was discarded. After a follow-up period of 6 months, one recipient recovered well while another gradually recovered after delayed graft function.Conclusions:This extraordinary case provides some references for selecting potential donors of mushroom poisoning. When the donor's relevant laboratory tests are normal, there is no pathological contraindication, sufficient time is available for estimating the type of mushroom poisoning, observing the trends of organ damage and waiting for the toxin clearance, donor with brain death from wild mushroom poisoning may donate organ.

Objective:Overweight and obesity prevalence has increased in low-income countries.This study systematically reviewed the obesity trend,disparities,and prevention and control efforts in Nepal. Methods:We searched PubMed and Google Scholar for articles and reports published between January 1,2004 and December 31,2022.Additional information on National policies and programs related to obesity prevention was retrieved from governmental websites and consultation with relevant experts.Overweight and obesity were defined using the World Health Organization body mass index cut points.Thirty-two studies and reports were included. Results:Overall,overweight and obesity rates increased in all groups in Nepal although nationally representative data remained limited.The combined overweight and obesity(OW/OB)and obesity rates in women aged 15-49 years increased from 8.5％to 22.2％and from 0.9％to 5.1％between 2006 and 2016,respectively.OW/OB and obesity rates in men were 17.1％and 2.5％based on data from the 2016 Demographic and Health Survey.OW/OB rate in under-five children increased from 0.6％to 2.8％between 2006 and 2016.Obesity rates for school-age(5-9 years)boys and girls in 2016 were 2.4％and 2.8％,respectively,and were 1.1％and 1.4％for male and female adolescents aged 10-19 years,respectively.OW/OB prevalence was much higher among women,residents in urban areas and central provinces,and in higher socioeconomic status groups.Projected prevalence of OW/OB and obesity for 2030 in adults aged 15-49 was 44.7％and 8.3％,respectively,while it was 2.2％for OW/OB in preschool children.Policies and direct interventions that specifically focused on obesity prevention and control are limited. Conclusions:OW/OB prevalence in Nepal has increased during the past 1.8 decades,disproportionately affecting population groups.Existing interventions mostly focused on undernutrition with some indirect implications for obesity prevention.In the future,Nepal needs to develop population-based programs for obesity prevention.