Objective To investigate the levels of IL-18,Th1(IL-2,TNF-?) and Th2(IL-4,IL-10) in patients with lung cancer and their relation to clinical pathological stages.Methods Totally 109 lung cancer patients were of 45 squamous cancer,40 adenocarcinoma,18 small cell lung cancer and 6 large cell carcinoma.Serum levels of IL-18,TNF-?,IL-2,IL-4 and IL-10 were determined by using an ELISA assay in 109 lung cancer patients and in 30 normal people.Results The levels of IL-18,TNF-? and IL-2 were significantly lower and the levels of IL-4 and IL-10 were significantly higher in lung cancer patients than in controls(P

Background Nitrosamines (NAms) are highly carcinogenic and frequently detected in drinking water systems in China, indicating potential human health risk through drinking water. Objective To analyze the distribution of NAms in drinking water in Shanghai and to evaluate relevant human health risk. Methods A total of 94 samples of source water and 120 samples of finished water were collected in February (dry period) and August (wet period) of 2021 from 30 drinking water plants in Shanghai, and eight NAms were quantitatively analyzed by gas chromatography tandem mass spectrometry after solid phase extraction. Cancer risks for different age groups caused by NAms exposure through water were evaluated using Monte Carlo probabilistic method and carcinogens risk assessment model recommended by the United States Environmental Protection Agency. Results The concentrations of total NAms in source and finished water were 12.56-65.86 ng·L⁻¹ and 8.52-57.38 ng·L⁻¹, respectively. The average concentration of total NAms was higher in source water (33.50 ng·L⁻¹) than in finished water (22.07 ng·L⁻¹, P<0.05) during dry season, and lower in source water (16.90 ng·L⁻¹) than in finished water (21.02 ng·L⁻¹, P<0.05) during wet season. The average concentration of total NAms in source water during dry season was twice of that during wet season. The positive rate of N-nitrosodimethylamine (NDMA) was the highest (100%) among the eight NAms. The cancer risk of exposure to NAms in finished water in Shanghai was mainly from the ingestion route, which was mostly caused by NDMA. The cancer risk from exposure to NAms in water for children (median=4.32×10⁻⁵) was slightly higher than that for adults (median=3.34×10⁻⁵) and adolescents (median=2.27×10⁻⁵). The cancer risks of exposure to NAms in water for people of different ages were mainly (80% - 95%) at an acceptable level (1×10⁻⁶ - 1×10⁻⁴). Conclusion NAms contamination is positive in Shanghai’s drinking water and NDMA is the main contaminant. The removal of NAms in water by current water treatment process is season-dependent. The cancer risk of children exposed to NAms in water was slightly higher than that of adults and adolescents, but all at acceptable levels.

Background Nitrosamines, especially N-nitrosodimethylamine (NDMA), are highly carcinogenic and frequently detected in drinking water systems in China, indicating potential human health risk through drinking water. Objective This study aims to analyze the distribution of NDMA in urban drinking water around China and to evaluate relevant human health risk, thus providing recommendations for drinking water safety standards. Methods The database of China National Knowledge Infrastructure was searched using nitrosamines and drinking water as key words in Chinese, and PubMed was searched usingN-nitrosodimethylamine or nitrosodimethylamine or NDMA, drinking water, and China as key words in English for relevant literature published from January 1, 2000 to March 1, 2021. Eligible data were extracted to statistically analyze the spatiotemporal exposure levels of NDMA in raw and finished water serving Chinese cities. According to the health risk assessment model for carcinogens recommended by the United States Environmental Protection Agency (US EPA), the cancer risk via drinking water of NDMA in finished water was evaluated for different age groups and different regions. Results A total of 102 studies were retrieved, 21 of which met the inclusion criteria and were finally included. The NDMA concentrations in 347 raw water samples and 276 finished water samples were extracted and studied, with positive rates of 82.4% and 67.0%, levels of not detected (ND)-141 and ND-67.1 ng·L⁻¹, and the 95th quantiles of 51.4 and 32.1 ng·L⁻¹, respectively. The level of NDMA in raw water was higher than that in finished water in cities of East and North China (P<0.05). East China had the highest average concentrations of NDMA in raw and finished water (21.4 and 9.0 ng·L⁻¹ respectively). In East China, the average concentrations of NDMA in raw and finished water from 2018 to 2019 (5.9 and 6.1 ng·L⁻¹ respectively) were significantly lower than those from 2015 to 2017 (28.7 and 16.5 ng·L⁻¹ respectively) (P<0.05). The average concentration of NDMA in rivers, lakes, ponds, and streams as drinking water sources (20.8 ng·L⁻¹) was significantly higher than that in groundwater sources (8.0 ng·L⁻¹) and in reservoir sources (6.5 ng·L⁻¹) (P<0.05). The average concentration of NDMA in finished water with pre-chlorination (9.4 ng·L⁻¹) was higher than that without (6.0 ng·L⁻¹) (P<0.05). The cancer risk of NDMA through drinking water for children over 1 year old in China decreased with older age, and the risk of infants aged 1~<2 years (2.52×10⁻⁴) was 23 times higher than that of adults (1.09×10⁻⁵) (P<0.05). Conclusion Raw water pollution is the main source of NDMA in urban drinking water in China, and current water treatment technology can partially remove NDMA. The NDMA level in finished water of East China shows a downward trend in recent two years. The carcinogenic risk of NDMA via drinking water for children is higher than that for adults, and their health risk management needs to be strengthened. The study findings recommend 35 ng·L⁻¹ as the limit of NDMA water quality safety standard in China.

Objective:To establish the biology reference interval (RI) of peripheral blood procalcitonin (PCT) for children between 3 days and 6 years old in China.Methods:Totally 3 353 reference individuals with apparent health or no specific diseases were recruited in 18 hospitals throughout the country during October 2020 to May 2021. Reference individuals were divided into four groups: 3-28 days, 29 days - 1 year, 1-3 years and 4-6 years. Vein blood or capillary blood were collected by percutaneous puncture from every reference individual. The PCT level in serum and the capillary whole blood were assayed by Roche Cobas e601 and Norman NRM411-S7 immunoanalyzer. Outliers were deleted and 95th percentiles of every group were provided as RIs. Man-Whitney U test or Kruskal-Wallis test were used performed to assess the difference among different gender, age or method groups. Results:The difference of PCT distribution between male and female is not statistically significant, but the difference between serum and capillary whole blood is statistically significant. The differences between age groups are significant too. For Roche e601, serum PCT RI of 3-28 days group is <0.23 μg/L, 29 days - 6 years are <0.11 μg/L. For NRM411, Serum PCT RI of 3-28 days group is <0.21 μg/L, 29 days - 1 year: <0.09 μg/L, 1 - 6 years: <0.10 μg/L. For whole blood PCT, RI of 3-28 days group is <0.26 μg/L, 29 days - 6 years is <0.15 μg/L.Conclusions:Serum and capillary whole blood PCT have different RIs, however, capillary whole blood PCT testing is valuable in pediatric application. Children in 3-28 days show higher PCT levels than other age group. To establish the RIs and understand the differences among different groups are essential for the interpretation and clinical application of peripheral blood PCT testing results.