OBJECTIVE: To explore an approach and method for the pharmaceutical care for COPD patients. METHODS: According to characteristics of clinical pharmacy in China, the approaches and methods of pharmaceutical care for COPD patients were established by referring to approaches for pharmaceutical care issued by Joint Accreditation Commission of Health Care Organizations. RESULTS&CONCLUSION:Pharmaceutical care could apparently improve drug use more safely, effectively and economically as well as prognosis for patients.

Objective To investigate the radiosensitivity enhancement on CL187 cancer cell lines after continous low-dose-rate irradiation of 125I seeds blocked by EGFR antibody.Methods There were control group,the irradiation or plus EGFR antibody group,with 3 samples in each group.Clongenic assay was used to detect the survival of cells.The cell cycle distribution and apoptosis were analyzed by flow cytometry.Results The survival fraction of CL187 cells were lower after blocked by EGFR antibody at the same dose irradiation.The SER were 1.34,1.59 and 1.98 when the antibody concentration were 2,5 and 10 nmol/L,respectively.The irradiation plus antibody led to more apoptosis(39.86%±4.38%)of CL187 cells than the irradiation (21.57%±2.97%)plused antibody(12.49%±1.59%)worked alone.Comparison of the low dose rate irradiation related to G2/M cell cycle arrest(46.41%±4.48%),More G1 cell cycle arrest(84.51%±3.42%)walked together along with the EGFR antibody.Conclusions EGFR antibody could enhance the cell radiosensitivity after continuous low-dose-rate.irradiation.The cell cycle redistribution and apoptosis might be the main mechanism of the radiosensitivity enhancement.

Objective To investigate the T cell clonality in patients with systemic lupus erythematosus (SLE) . Methods T cell clonality in peripheral blood lymphocytes from six patients with active SLE was analyzed using reverse transcriptase- polymerase chain reaction (RT- PCR) and GeneScan technique. Results T cell receptor (TCR) Vβ PCR products from two patients were derived from polyclonal T cells, however, TCR Vβ9, Vβ2, VβI, Vβ2 pioducts from the other four patients were derived from oligoclonal T cells respectively.ConclusionT cells from patients with SLE demonstrated a striking oligoclnality and their clonal expansion may be a specific response driven by autoantigens.

Malignant tumors of the skull base are rare,present with a variation of histologic stypes, and don't have specific symptoms. Their treatment paradigms differ with histology,location and stage. Many times it is essential to need multidisciplinary cooperation for dealing them. A better understangding of malignant tumors of the skull base will be beneficial for standardized treatment.

Objective To analyze the sequence of the novel conjugative plasmid pO157_Sal detected in outbreak isolates of Escherichia coli O157∶H7 .Methods The traE genes of the outbreak isolates in China were amplified by polymerase chain reaction (PCR) and the products were sequenced .The TraE sequences of Escherichia coli O157 ∶ H7 strains from other sources were retrieved from GenBank . Phylogenetic tree based on the TraE sequences was constructed by Neibhor-joining analysis .The whole plasmid sequences of pO157_Sal and pEC4115 were compared .Results The sequences of traE gene were identical among the Chinese isolates . There were homologous sequences of TraE in Escherichia coli O157∶H7 isolates from different sources .Twenty-one out of the 52 pO157_Sal genes were homologous to genes of pEC4115 with amino acid level identity ranging from 28% to 51% .Conclusions Although similar TraE sequences and similar plasmid are found in Escherichia coli O157∶H7 isolates from different sources ,pO157_Sal is only observed in Chinese outbreak isolates .The TraE sequences are conservative among the outbreak isolates ,indicating they are from the same specific source .

Higher mathematics teaching has for long been regarded as the instruction of pure mathematical theory and knowledge,which lacks the enlightenment of humanistic thoughts.This paper innovatively introduces humanistic thoughts and spirit into the teaching process of higher mathematics,which propels the lecture teaching effect of higher mathematics,enhances the teaching efficiency of higher mathematics,and promotes the humanistic quality of medical students.

The investigation on the leaves of Morus alba L. was carried out to find the relationship of the constituents and the pharmacological activities. The isolation and purification were performed by various chromatographies such as silica gel, Sephadex LH-20, RP-C18 column chromatography and so on. Further detailed investigation on the fraction of the ethanol extract of leaves of Morus alba yielded four Diels-Alder type adducts mulberrofuran F1 (1), mulberrofuran F (2), chalcomoracin (3), kuwanon J (4), together with two chalcones morachalcone A (5), isobavachalcone (6), and three flavones norartocarpetin (7), kuwanon C (8), 6-geranylapigenin (9). Their structures were elucidated by the spectral analysis such as NMR, MS etc. Compounds 1, 6 were isolated from this plant for the first time, compounds 4-5, 7-9 were isolated from the leaves of Morus alba L. for the first time, among which 1 was a new compound. Compounds 1-5 were evaluated for the cytotoxicity against A549, Be17402, BGC823, HCT-8 and A2780 cell lines in vitro by MTT method, but only compounds 1-3 showed cytotoxicity against several human cancer cell lines.

Objective To evaluate the medical students' medical professionalism.Methods Totally 1200 medical college students from first grade to fourth were randomly selected to do the self-designed questionnaire of medical occupation spirit using the method of cluster sampling.Principal component analysis was applied to do data processing and analysis.Results ①Two principle components were extracted,the first principal component included the attitude on the personal contact with patient,attitude on the in-depth clinical practice aiming at improving the ability of doctor-patient communication,attitude on playing multiple roles in contacting patient and the understanding of the physician-patient relationship.The second principal component included the working attitude toward history collection,the sense of pride when be praised “proficiency” and the attitude towards difficult operations.② The scores of medical occupation spirit for medical students of first grade to four grade were:-4.4226,-0.0343,-5.1397,0.6827.The score was the highest in grade three medical students followed by grade two and grade one.Conclusion Unstable development trend was observed in medical occupation spirit among medical students and due attention should be paid.Strengthening the medical studenl occupation spirit education and training should focus on two aspects:the subjective aspect,namely active communication and exchange with patients who have subjective dynamic consciousness; the objective aspect,namely the attitude towards the grasp of professional skills.

Itraconazole has potent anticancer activity at the standard therapeutic doses,which is proved in vitro and in vivo preclinical studies,even in active clinical trials.The precise mode of action of itraconazole for its anticancer activity has not been elucidated,however multiple putative mechanisms are proposed,such as anti-angiogenesis,inhibition of Hedgehog pathway,autophagy induction and reversal of multi-drug resistance.Its high efficacy,known toxicity and well-estabhshed pharmacokinetics make this generic drug a strong candidate for repurposing as an oncological treatment.

Proteomics is a technology to study all the proteins encoded by gene.It has become a useful tool for analyzing the expression changes of proteins,and has been widely used in research of gastrointestinal tumors in recent years.Through kinds of techniques such as two-dimensional gel electrophoresis,some differentially expressed proteins correlated with patients prognosis were found,which may served as the possible biomarkers of gastroin testinal tumors in future.It helped to find some certain proteins related to tumor cell nutritional intake,cell metabolism,cell adhesion,cell migration and cytoskeleton remodeling which may play the important roles in development of gastrointestinal tumor.It also helped to explain the drug resistance mechacism of chemotherapy and molecular targeted theraphy for gastrointestinal tumors.Proteomics technology plays an increasingly important role in the basic research and clinical services for gastrointestinal tumors.