Objective To discuss the clinical characteristics and treatment of children with primary splenic tumor.Method A retrospective analysis was made on 13 children with primary splenic tumor from January 1970 to December 2001.Results There were splenic hemangioma in 5,splenic lymphoma in 4,splenic cysts in 2 and splenic malignant lymphoma in 2.In the primary splenic benign tumors,4 cases were treated with splenectomy,7 cases were treated with partily splenectomy;2 cases of splenectomy often had respiratory tract infection 1 year postoperatively.2 of the splenic malignant lymphoma were treated with splenectomy and chemotheraphy postoperatively;1 of them died 23 months postoperatively,another is still alive 8 months postoperatively.Conclusions The diagnosis of primary splenic tumor in children is mainly depended on B-ultrasound examination and CT scanning.Children with primary splenic benign tumors should be retained normal spleen as far as possible intraoperatively.It is important for splenectomy to pay more attention to the clinical infection 2 years postoperatively.Splenectomy combined with chemotherapy may provide optimum therapy for children with primary splenic malignant lymphoma.Early detection and treatment are crucial to increase the survival rate of children with primary splenic malignant lymphoma.

0.05).(2) After treatment for 7 days,APACHEⅡ score was decreased in the two groups(P

Objective To evaluate the feasibility and effectiveness of laparoscopic-assisted modified Swenson’s operation for Hirschsprung’s disease in infants. Methods A total of 13 infants with Hirschsprung’s disease including 11 males and 2 females were treated by laparoscopic-assisted modified Swenson’s surgery. Three- or four-trocar technique was used under a CO2 pressure of 8-10 mm Hg. The seromuscular layer of the colon was obtained for pathological examination. The mesenteries of the sigmoid colon and rectum were dissected by Ligasure, and the rectum was dissected down to 0.5-1.0 cm above the dentate line. Through the anus, the angusty and transmigration segment of the intestinal canal were evaginated, pulled out, and resected. Whole-layer colon-rectum anastomosis was performed,?21 mm stapler was used in 3 cases. Results All the operations were completed with laparoscopy in 85-161 min (mean, 115 min). The blood loss was less than 12 ml in all the patients. No intraoperative complications occurred. After the operation, 3 patients developed intestinal inflammation, 1 had anastomotic leakage, and 1 feces stain. All the compilations were cured by conservative treatments. The patients were followed up for 6 to 42 months (mean, 26 months), during which no one had complications. Conclusion Laparoscopic-assisted modified Swenson’s operation is feasible and effective for Hirschsprung’s disease in infants.

This study was aimed to observe the effect of Tao-He Cheng-Qi Decoction (THCQD) on tissue injury of different organs among rats with sepsis. A total of 100 Kunming male SD rats of clean grade were randomly divided into five groups, which were the normal control group, model 6 h control group, model 12 h control group, THCQD 6 h group and THCQD 12 h group. The normal control group received no treatment. The classic CLP method was ap-plied in the establishment of sepsis rat model in other groups. Intragastric administration of saline 1 ml/100 g (weight) was given to each rat in the model group every day. Intragastric administration of THCQD 1 ml/100 g (weight) was given to each rat in the THCQD group every day. The model was established after 7 days. Tissue speci-mens of lung, heart, kidney, liver, and small intestine were collected at 6 h and 12 h after modeling, respectively. Pathomorphological changes of each group were observed by light microscopy and electron microscope. The results showed that compared with the model group at the same time point, pathomorphological changes of tissues of the lung, heart, kidney in THCQD group were not significant. However, pathomorphology of tissues of the liver and small intestine changed significantly in the THCQD 12h group. And there were statistical differences (P < 0.05) between two groups in the score of pathomorphology. It was concluded that THCQD had protective effect on tissues of the liv-er and small intestine among rats with sepsis. However, the mechanism is not clear and requires further research.

To simultaneously determine the contents and explore the pharmacokinetics of ferulic acid and paeoniflorin by high performance liquid chromatography (HPLC) after oral administration of Modified Xiao-yao Decoction (MXYD), a compound of traditional Chinese herbal medicine.

Objective To evaluate the dosimetry of high-energy electron beams by using GafChromic EBT film. Methods The percent depth doses of electron beams of 4 MeV,6 MeV,8 MeV,10MeV, 12 MeV and 15 MeV were measured with EBT. The results were then compared with the measurements with diode detector in RFA and parallel plate chamber in water tank. Results The percent depth dose curves using EBT film had a good agreement with those using the other two detectors. Furthermore, no differences were found between up-right and tilt setup methods. When film upper edge is higher than water surface, a sharp drop of measurement results using EBT film was observed in comparison with those from diode detector in build-up region. Conclusions EBT film can be applied to measure percent depth doses of high-energy electron beams. During the EBT film measurements, the film should be tilted at the angle of 5degrees to the central axis of the field. When choosing up-right setup method, the edge of the film should be parallel to the water surface.

Objective:To inspect the dosimetry characteristic of a 2-D ionization chamber array and its response to oblique incident electron beam.And to discuss feasibility to using it to validate the electron arc therapy plan.Materials and methods:(1)The 2-D ionization chamber array was calibrated referring to a themble chamber,comparing the response to electron beams with incidence angle ranged from-50°to 50°.(2)Phantom test plans of 6 and 10MeV electron beam arc therapy were designed,with the beam rotation arcs of 30°,60°and 90° respectively,symmetric to the Central axis of 0°gantry angle.Dose distribution of each plan was verifled with the 2-D array.Results:(1)Measurement differences between the central detector of the array and the thimble chamber were less than 2%,under the planned oblique inddent 6/10MeV electron beam.(2)For the all test plans,dose error was less than 3%at dmax in the symmetric axis of the electron beam arcs.Off-axis dose error was less than 2%within the central 70%area of the profile in non-rotating direction,and smaller than 1.5%in the rotational direction.The measured dose distribution had good agreement with the TPS calculation in isodose curves between 100%and 20%.The gammapass rates(△D=5%,△d=5cm)were 99.98%、99.89%、99.74%、98.64%、99.16%and 99.44%respectively for 6 and 10 MeV plans with electron beam arcs of 30°,60°and 90°.Conclusion:it is practicable using the tested 2-D ionization chamber array to verify and validate the electron beam arc therapy plan.

BACKGROUND:It may be an important approach to avoiding organ transplant rejection by utilizing immature dendritic cells to induce donor-specific immunologic tolerance. OBJECTIVE:To study the effect of sinomenine on the differentiation and maturation of rat bone marrow-derived dendritic celsin vitro. METHODS:Bone marrow-derived dendritic cells were isolated from the rat femur and tibia, and immature dendritic cells were induced by granulocyte-macrophage colony stimulating factor and interleukin-4. On day 7, lipopolysaccharide was added and the cells were cultured to generate mature dendritic cells. Cells were divided into control group and low-, middle- and high-dose sinomenine treatment groups (SNL, SNM, SNH groups). Forty hours later, dendritic cels were harvested, and cell morphology was observed by inverted phase contrast microscope. The expression of CD80 and RT1B was detected by flow cytometry. ELISA was used to detect the expression of interleukin-12. The mixed lymphocyte reaction was used to detect the ability of dendritic cells to stimulate the activation of allogeneic T lymphocytes. RESULTS AND CONCLUSION: (1) Under the inverted microscope, the morphology of mature dendritic cells was observed in the control group; in the SNL group most dendritic cells were visible; in the SNM group, there were partially suspended cells with poor maturation; and in the SNH group, most of the cells were not mature. (2) The expression of CD80 in the control group was significantly lower than that in the SNL, SNM and SNH groups (P < 0.05), and the expression of RT1B was significantly reduced in the SNM and SNH groups than the control group. (3) Compared with the control group, the level of IL-12p70 in the cell supernatant was significantly decreased in the SNM and SNH groups (P < 0.01). (4) The ability of dendritic cells to stimulate T lymphocyte proliferation in the SNM and SNH groups was significantly decreased compared with the control group (P < 0.05). To conclude, sinomenine can inhibit the maturation of dendritic cells.

Objective To evaluate the relevance of PET CO 2 ,TcPCO 2 and PaCO 2 in pediatric patients with congenital heart disease.Methods Eighty pediatric patients with congenital heart disease were scheduled for elective cardiac operation with cardiopulmonary bypass,aged 1 month to 5 years, 42 male and 38 female,were divided into 4 groups:group N1,cyanotic type congenital heart disease with severe pulmonary hypertension;group N2,cyanotic type congenital heart disease without pul-monary hypertension;group N3,non-cyanotic congenital heart disease with severe pulmonary hyper-tension;group N4,non-cyanotic congenital heart disease with mild pulmonary hypertension or normal of pulmonary artery pressure,20 cases in each group.The same anesthesia methods were selected in each group.PET CO 2 ,TcPCO 2 and PaCO 2 before operation were recorded,and the relevance among them was analyzed.Results In groups N1,N2 and N3,the differences of PET CO 2 and PaCO 2 were significant,,while the difference was relatively small in group N4;the difference of TcPCO 2 and PaCO 2 were relatively small between the four groups.In Groups N1,N2 and N3,the values of TcP-CO 2-PaCO 2 value were significantly less than that of PET CO 2-PaCO 2 ;in group N4,TcPCO 2-PaCO 2 value was obviously higher than that of PET CO 2-PaCO 2 values (P < 0.01 ).Correlation analysis of PET CO 2 and PaCO 2 showed that R2 in the groups N1,N2,N3,N4 were 0.028,0.1 97,0.390, 0.71 1(P >0.05,0.02