Objective To investigate the correlation between NRAMP1 gene polymorphisms and susceptibility to tuberculosis ( TB) in Tibetan people in Qinghai. Methods A case-control study was con-ducted in this study, involving 99 Tibetan patients with TB and 89 healthy Tibetans. The single nucleotide polymorphisms of NRAMP1 gene at rs17235409 and rs3731865 sites were detected by using TaqMan probe method. Gene cloning and sequencing typing were performed to analyze the single nucleotide polymorphisms of NRAMP1 gene at the rs17235416 site. SPASS20. 0 software was used to statistically analyze the correla-tion between NRAMP1 gene polymorphisms and susceptibility to TB in Tibetan people. Results No signifi-cant difference in the genotype frequencies of rs3731865 and rs17235409 was found between the two groups (χ2=0. 852, P=0. 356;χ2=0. 279, P=0. 597). The genotype frequencies of TGTG/TGTG and TGTG/del+del/del at the rs17235416 site were 70. 7% ( 70/99 ) and 29. 3% ( 29/99 ) in patients with TB and 86. 5% (77/89) and 13. 5% (12/89) in healthy subjects. There were significant differences in the geno-type frequencies of TGTG/TGTG and TGTG/del+del/del between the two groups (χ2=6. 870, P=0. 009). The genotypes of TGTG/del and del/del at rs17235416 were risk factors for TB ( OR=0. 376; 95%CI:0. 178-1. 794 as compared with the TGTG/TGTG genotype in Tibetan people in Qinghai. Conclusion This study suggested that the NRAMP1 gene polymorphisms at rs3731865 and rs1723409 sites had no correlation with the susceptibility to TB in Tibetans in Qinghai. However, the NRAMP1 gene polymorphisms at rs17235416 site were correlated with the susceptibility to TB. The TGTG/del alleles at the rs17235416 site might be the risk factors for tuberculosis in Tibetans in Qinghai.

Paeonia Radix Alba is the dried root of Paeonia lactiflora Pall.in the ranunculus family,which is mainly produced in Zhejiang(Hang Paeony),Anhui(Hao Paeony)and Sichuan(Chuan Paeony).It has the effects of calming liver,relieving pain,nourishing blood,regulating menstrual period,collecting Yin,and preventing perspiration.Similar to Paeonia Radix Alba,Paeonia Radix Rubra is the dried root of Paeonia lactiflora Pall.or Paeonia veitchii Lynch.in the ranunculus family.Although the chemical constituents are similar,the efficacy and clinical application of the two herbal medicines are obviously different owing to their various processing methods and techniques.Modern studies have shown that Paeonia Radix Alba is rich in multiple chemical constituents,mainly including monoterpenoids and their glycosides,triterpenoids and their glycosides,volatile oils,flavonoids,and polyphenols,and has a wide range of pharmacological effects such as analgesia,anti-inflammation,anti-depression,liver protection,anti-tumor,and anti-hyperglycemia.This paper reviews the progress of research on effective constituents of Paeonia Radix Alba and their pharmacological effects to provide references for the comprehensive development and utilization of Paeonia Radix Alba.