AimTo realize the regulatory actions of nitric oxide(NO) on supraoptic nucleus (SON) neurons by observing the effects of NO donor sodium nitroprusside(SNP) on SON neurons. Methods The cell electrophysioloical properties were obtained by the intracellular recording techniques from the SON neurons in adult rat hypothalamic slices. Results In 11 silent cells, superfusion of SNP(1 mmol · L-1) for 3～5 min resulted in the depolarization response with a decrease of membrane resistance and time constant(P＜0.05) in 55% of cells, while hyperpolarization with an increase of membrane resistance in 45 % of neurons. In 73% of tested cells, SNP also decreased the amplitude, overshoot, firing frequency and slope of current-voltage curve, and increased the afterhyperpolarization of action potentials evoked by intracellular depolarizing current pulses(P ＜0.05).However, SNP enhanced the spontaneous firing in 67% of 6 tested cells with spontaneous activities. ConclusionSNP presents an inhibitory or excitatory effect on SON neurons in aneurontype-andfunctionalstate-dependent manner, which suggests that NO may exhibit adverse regulatory actions on SON neurons.

Objective To understand the prevalence condition of occupational musculoskeletal disorders in nurses,so as to supply scientific evidence for reducing and preventing nurses′occupational injuries.Methods 582 nurses from three level three class -A hospital in Wenzhou were investigated,which were selected by convenience sampling method.Results The prevalence of musculoskeletal disorders was 76.3%.The prevalence of low back pain was highest,which was 58.9%.Among the nurses who had lower back pain,in the past 1 year,43.4%of them had a total duration of illness more than 30 days,18.7% of them reduced daily activities time for more than 30 days,and 22.6% of them had received examination and treatment.The main factors which related to low back pain among nurses were age (OR =2.18,95% CI:1.36 -3.67 ),stoop time (OR =2.24,95% CI:1.08 ~4.36 ), occupational stress(OR =1.82,95%CI:1.14 ~2.89),work fatigue(OR =2.45,95%CI:1.27 ~3.15)and physical exercise(OR =0.63,95%CI:0.44 ~0.89).Conclusion The condition of occupational musculoskeletal disorders is serious,especially low back pain.Interventions related to influencing factors can be taken to reduce the incidence of low back pain.

Aim To realize the modulatory actions of nitric oxide(NO) on supraoptic nucleus(SON)neurons by observing the effects of NO donor sodium nitroprusside(SNP) on the SON neurons. Methods The cell electrophysiology properties were obtained by the intracellular recording techniques from the SON neurons in adult rat hypothalamic slices. Results Local pressure ejection of glutamate induced depolarizing responses with decrease of input resistance, concentration- and membrane potential-dependent properties in 12 SON neurons. The amplitude and duration of glutamate responses were suppressed by bath of SNP in 67% of tested cells(P

ObjectiveTo study the clinical application value of screening cervical precancerous lesions of high risk human papillomavirus(HPV) testing combined with thin-prep cell test(TCT) in prevention of cervical intraepithelial neoplasma (CIN) and cervical carcinoma. MethodsSeven hundred and sixty-three women were screened by high risk HPV test, TCT and pathology test of biopsy more. The positive coincident rate with the pathology test positive rate during the three technologies was compared.ResultsHigh risk HPV positive was 287 cases, the rate of infection was 37.61％(287/763), and pathology test positive was 82 cases, the coincident rate of which was 28.57％ ( 82/287 ) ; TCT positive was 78 cases, the rate of infection was 10.22％ (78/763), and pathology test positive was 50 cases, the coincident rate of which was 64.10％ (50/78) ; TCT + high risk HPV positive was 59 cases, pathology test positive was 47 cases, and the coincident rate of which was 79.66％ (47/59). Among each coincident rate,the differences were statistically significant (P＜ 0.05). The positive rate of high risk HPV infection CIN I was 32.26％( 10/31 ),CIN Ⅱ 41.38％ (12/29), CIN Ⅲ 93.55％ (29/31), squamous carcinoma of the cervix 96.88％ (31/32),respectively. ConclusionsThe positive rate of high risk HPV increases significantly with the grade of cervical precancerous lesions. It is of great significance to screen in the population for HPV and TCT in a regular time span,since it could not only decrease HPV infection rate and the incidence of cervical lesions,but also prevent the cervical cancer.

Three compounds were isolated from the leaves of Rabdosia amethystoides (Benth) C.Y.Wu et Hsuan. They were identified as Irabdosinaiol (Ⅰ), oleauolic acid (Ⅱ), ?-sitostcrol (Ⅲ) on the basis of chcmical reactions and spectral data. compounds Ⅰ and Ⅱ were isolated for the first time from this plant.

Objective To explore the changes and clinical significance of serum cystatin-C, β2- mieroglobulin (β2-MG) and renal hemodynamic in children with Henoch-Schoenlein purpura(HSP). Method Forty-six patients with HSP are in HSP group, 40 healthy children are in control group. Serum cystatin-C was determined by enzyme-linked immunosorbent assay, β2-MG was detected by radioimmunoassay, renal hemodynamie was detected by colour Doppler ultrasound. Results Serum cystafin-C and β2-MG in HSP group [(3.96±1.52 ), (2.74±0.82)mg/L] were higher than those in control group [(1.67±0.61), (1.89±0.47)mg/L] (P<0.01). Frequenee spectra showed high velocity and resistance, and maximum crest flow rate[(1.068±0.348) m/s] and resistance index (0.894±0.125) in systolic phase of main renal arteries were obviously higher in HSP group than those in control group [(0.859±0.357) m/s and 0.726±0.078] (P<0.05). Conclusions The level of serum cystatin-C and the change of renal hemedynamie can act as the significant indicators of early diagnosis of HSP nephritis.

Objective To analyze the behavioral characteristics and mental health status for patients with chronic hepatitis B,to explore the correlation between them and analyze the influence of behavioral characteristics of patients with chronic hepatitis B on their mental health.And provide a reference of clinical mental health intervention for patients with chronic hepatitis B.Methods 216 cases of patients with chronic hepatitis B in our hospital from October 2010 to October 2011 were chosen as the research object.Symptom checklist-90(SCL-90)and Eysenck personality questionnaire(EPQ)were used for evaluation.The correlation of behavioral characteristics and mental health status for patients with chronic hepatitis B and the effect of behavioral characteristics of patients with chronic hepatitis B for their mental health were analyzed.Results The SCL-90 score for 216 cases of chronic hepatitis B patients showed that its total score was significantly higher than the norm,the difference was statistically significant.The results of EPQ score showed that the neurotic or emotional stability of personality scores were significantly higher than the norm,the difference was significant.The psychotic scores were significantly lower than the norm,the difference was significant.The analysis of the correlation for nine projects in the SCL-90 scale and four items in the EPQ scale showed that introversion had a significant positive correlation with compulsion,depression,anxiety,fear and paranoia.The nervous or emotion had a significant positive correlation with compulsion,interpersonal sensitivity,paranoid,psychotic.Conclusions The behavioral characteristics of chronic hepatitis B patients have a relation with the mental health.The mental health status changes according to the changes of behavioral characteristics.

Ebola virus(EBOV)and Marburg virus(MARV),belonging to the Filoviridae family,emerged four decades ago and caused severe viral hemorrhagic fever in human and other primates.As high as 50-90% mortality,filoviruses can cause significant threats to public health.However,so far no specific and efficient vaccine has been available,nor have other treatment methods proved to be effective.It is of great importance to detect these pathogens specific,rapidly and sensitively in order to control future filovirus outbreaks.Here,recent progresses in the development of detection and diagnosis methods for EBOV and MARV are summarized.

Objective To investigate the infection of human cytomegalovirus (HCMV) in patients with atherosclerosis and explore its clinical significance.Methods A total of 134 patients with atherosclerosis (observation group) including 102 patients with coronary heart disease (CHD) and 32 patients with non-CHD from December 2009 to April 2012 were enrolled in this study.The 102 patients with CHD were divided into HCMV infection group (86 cases) and non-HCMV infection group (16 cases).Another 40 healthy person were selected as control group.The carotid intima-media thickness (IMT) was assessed by ultrasonography.The serum HCMV-IgM was measured by enzyme-linked immunosorbent assay.Results The infection rate of HCMV in observation group was 77.6％ (104/134),which was higher than that of 37.5％(15/40) in control group(P ＜ 0.05).The infection rate of HCMV in CHD patients and non-CHD patients was 84.3％(86/102) and 56.2％(18/32),and there was significant difference (P ＜ 0.05).The carotid IMT in HCMV infected atherosclerosis patients was (1.31 + 0.28) mm,which was higher than that of (1.14 ± 0.21)mm in non-HCMV infected atherosclerosis patients (P ＜ 0.05).The incidence of myocardial infarction and multi-vessel lesion in HCMV infection group was higher than that in non-HCMV infection group [39.5％(34/86) vs.25.0％(4/16) and 43.0％(37/86) vs.18.8％(3/16)](P＜ 0.05).The incidence of stable angina pectoris and single-vessel lesion in HCMV infection group was lower than that in non-HCMV infection group [26.7％(23/86) vs.43.8％(7/16) and 22.1％(19/86) vs.56.2％(9/16)](P＜0.05).Conclusion The high-infection rate of HCMV is found in atherosclerosis patients,and it maybe have a close relationship with the occurrence and development of atherosclerosis.

Objective:To establish a bacterial endotoxins testing method for L-oxiracetam injection. Methods: According to the gel-clot technique for bacterial endotoxin inspection and the related regulations in Chinese Pharmacopoeia (2015 edition, volume Ⅳ, general rule 1143), tachypleus amebocyte lysate (TAL) from two different manufacturers were used for bacterial endotoxins test on 3 batches of L-oxiracetam injection with methodology studies. Results:Diluted to 8. 3 mg·ml-1 , the samples showed no interference a-gainst the bacterial endotoxins test. Conclusion:The bacterial endotoxins test method for L-oxiracetam injection is applicable with the endotoxin limit of 3 EU·ml-1 .