ObjectivesTo assess the cardiovascular function in obese children.MethodsA total of 30 obese children and 50 age- and sex-matched normal weight children were enrolled and treadmill test was performed. Exercise tolerance, the time to target heart rate and continuous T-wave alternans (TWA) during treadmill test were compared between two groups. ResultsCompared with normal weight children, the exercise tolerance was decreased and the time to target heart rate was shortened, while the incidence of continuous TWA was increased in obese children and there was signiifcant difference between two groups (P<0.01).ConclusionsObesity may lead to cardiovascular dysfunction in childhood which should be concerned.

Objective To explore the effect of ulinastatin trypin inhibitor on inhibiting pancreatic secretion and preventing and curing pancreatic fistula in patients underwent pancreatodudenectomy.Methods 45 cases underwent pancreatodudenectomy were not given ulinastatin as control group postoperatively,32 patients underwent pancreatodudenectomy were intravenously driped by ulinastatin 100,000u solution in physiologic saline,every 12 hours (Bid) for 5～8days.Child's pancreatodudenectomy was adopted in all the cases. Results Pancreatic secretion at postoperative 120h in ulinastatin group (138 26?114 74)ml/d were significantly lower than those of control group (248 56?106 75)ml/d (P

Objective To investigate the causes,preventive measures and treatment of digestive tract injury during laparoscopic cholecystectomy (LC). Methods From January 2000 to December 2007,totally 21 640 patients underwent LC in our hospital,among them,16 had digestive tract injury including injuries in the stomach and duodenum in 14 patients,in the colon in 1,and in the ileum in 1. Results Among the 16 cases,13 were detected during the operation,and thus laparoscopic repair were performed on 3 of the cases,and conversion to open surgery were carried out for the other 10 for repair of the injured tract (7 cases) or major resection of the stomach and Roux-en-Y anastomosis (3 cases). The other 3 patients were confirmed after LC;they received open surgery for repair of the ileum,colostomy or intra-abdominal cavity drainage. In the 16 cases,one patient died of duodenal leakage complicated with multiple organ dysfunction syndrome in 7 days after the operation;the other 15 patients were cured and discharged from hospital without severe complications. Conclusions Most of the digestive tract injury cases during LC are caused by pericholecystic inflammatory adhesion,which leads to insufficient exposure of the surgical field resulting in the tear or perforation of the digestive tract. Proper treatment in early stage is the key to the prognosis of the patients and prevention of severe complications.

Objective To explore the oxidative damage and genotoxicity of organic chemical pollutants from running water and the source water in Baotou reach of the Yellow River to mice. Methods The Kunming mice were treated with the organic extract solutions of the Yellow River water and running water by gavage at different concentration,once a day and the activity of the superoxide dismutase (SOD), glutathione peroxidase (GSH-Px) and the content of malondialdehyde (MDA) in the liver and kidney and the rate of micronucleus were determined. Results SOD and GSH-Px activity in the liver and kidney in the groups treated with the water of three sections of Yellow River in Baotou City decreased as the exposure dose increased. Except the low concentration group of running water,the MDA content in the liver and kidney and the micronucleus rate increased significantly compared with the control (P

OBJECTIVE: To observe the therapeutic efficacy of compressed atomized inhalation of Tanreqing injection for herpangina.METHODS: A total of 137 children with herpangina were enrolled: 69 cases(trial group) received Tanreqing injection 0.5 mL?kg-1?d-1 plus 5 mL normal saline q.d by atomized inhalation for 5 consecutive days,and 68(control group) received penicilin G sodium and ribavirin by iv gtt.The curative efficacy of the two groups was followed.RESULTS: There were significant differences between the trial group and the control group in total effective rate and marked effective rate(85.51 % vs.95.65% for total effective rate and 27.94% vs.66.18% for marked effective rate,P

Objective To focus on the causes and management of complications after laparoscopic cholecystctomy (LC). Methods A retrospective analysis of clinical records of 13 278 patients undergoing LC in this hospital from March 1991 to July 2003 was made. Results Complications occurred in 110 patients (0.83%), involving 19 cases of bile duct injuries (0.14%), 37 cases of bile leakage (0.28%), 31 cases of residual calculi in common bile duct (0.23%), 4 cases of intraabdominal hemorrhage (0.03%), 5 cases of gastrointestinal injury (0.04%), 3 cases of intraabdominal abscess (0.02%), 6 cases of incisional infection or hernia (0.05%) and 5 cases of serious subcutaneous emphysema (0.04%), respectively. Five patients died (0.04%). Conclusions The major complications after LC include bile duct injury, bile leakage and residual calculus. Most of complications are preventable and curable.

Objective To summarize the experience in prevention and treatment of bile duct injury during laparoscopic cholecysteclomy(LC). Methods Retrospective analysis on the clinical data of 31 cases of LC was carried out. Of them, 27 underwent one stage operative repair and 4 underwent palliative external drainage of the bile duct first. Results 16 cases succeeded and 11 failed in the one stage operative repairing.There were 15 cases underwent 2～4 operations in this series,and the re-operation rate was 48.4%. 29 patients was cured while 2 died. Conclusions During LC, the operative rule should always be strictly observed, and the key to preventing bile duct injury is to avoid clipping, cutting and coagulation blindly.

Objective:To explore the relationships of pathology of phlegm syndrome of TCM with blood sugar,insulin and insulin resistance in metabolic syndrome(MS).Methods:The information of 233 cases of MS was collected by four diagnostic methods,syndrome differentiation by syndrome element was used to analyze the diagnosis information,and the blood glucose,insulin and HOMA-IR were detected.Results:①Fasting blood glucose and 30 min,60 min,120min,180 min postprandial blood glucose level had positive correlation with phlegm.Correlation coefficient were 0.158(P

Zishi township of Jiangling city is located in the canal area inside embankment,a heavy endemic area of schistosomiasis japonica in Hubei province. The population and cattle preva-lence was 29.5% and 66.7% respectively in 1992. There are 66 canals and it covered a total area of 300000m2. 97.0% of the canals and 99.3% of the total area had snail inhabitants, and 7.6% of the snails distributed in canal was infected which covered 18.3% of the total area. The snails were mainly distributed in the main canal and their branches. The rate of snail distribution was 45.2% and 39.8% respectively. Cattle was pastured all year round in this area. Examination of scattered cattle feces and water revealed that the major factor of water contamination was scattered cattle feces. It explained why the infection rate of snail was high and the distribution of infected snail was extensive and how the major high rise area inside embankment was formed.

This study examined the effect of self-microemulsiflying drug delivery system (SMEDDS) containing Cremophor RH40 or Tween 80 at various dilutions on cytochrome P450 3A (CYP3A) enzymes in rat hepatocytes, with midazolam serving as a CYP3A substrate. The particle size and zeta potential of microemulsions were evaluated upon dilution with aqueous medium. In vitro release was detected by a dialysis method in reverse. The effects of SMEDDS at different dilutions and surfactants at different concentrations on the metabolism of MDZ were investigated in murine hepatocytes. The cytotoxicity of SMEDDS at different dilutions was measured by LDH release and MTT technique. The effects of SMEDDS on the CYP3A enzymes activity were determined by Western blotting. Our results showed that dilution had less effect on the particle size and zeta potential in the range from 1:25 to 1:500. The MDZ was completely released in 10 h. A significant decrease in the formation of 1'-OH-MDZ in rat hepatocytes was observed after treatment with both SMEDDS at dilutions ranging from 1:50 to 1:250 and Cremophor RH 40 or Tween 80 at concentrations ranging from 0.1% to 1% (w/v), with no cytotoxicity observed. A significant decrease in CYP3A protein expression was observed in cells by Western blotting in the presence of either Cremophor RH40 or Tween 80-based SMEDDS at the dilutions ranging from 1:50 to 1:250. This study suggested that the excipient inhibitor-based formulation is a potential protective platform for decreasing metabolism of sensitive drugs that are CYP3A substrates.