Objective To explore the epidemiological and clinical feature of hepatitis B virus and hepatitis C virus (HBV/HCV) co-infection in Guangzhou area,and provide evidence to elevate the diagnoses and treatment for the co-infection.Methods Data of patients with chronic HBV infection seen from April 2005 to October 2011 were retrospectively collected,and the epidemiological features of HBV/ HCV co-infection were analyzed.Patients with HBV/HCV co-infection were stratified according to the HBeAg status and the levels of HBV DNA and HCV RNA,and their clinical feature were analyzed.Results The prevalence of HBV/HCV co-infection was 1.9％ (128/6604),which was mainly seen in middle age of male populations with history of drug addiction.There were no significant differences in biochemical markers and percentage of end-stage liver disease between HBeAg positive and HBeAg negative co-infection patients.The HBV DNA positive rate in HBeAg positive patients was higher than those in HBeAg negative patients (P ＜ 0.001),while the HCV RNA positive rate was lower than those in HBeAg negative patients (P =0.007).The frequency of end-stage liver disease in HBV DNA positive patients was higher than that in HBV DNA negative patients (58.1％ vs.37.9％,P =0.027),but it was similar between HCV RNA positive and negative patients (43.6％ vs.49.2％,P =0.540).Conclusions The prevalence of HBV/ HCV co-infection in Guangzhou area in low,and the levels of HBV DNA may associate with progression of disease.