Objective To prove the feasibility of a Fogging Process in fixing high-concentration radioactive aerosol ¹³¹I contamination. Methods High-concentration radioactive aerosol ¹³¹I contamination in an ¹³¹I-operating glovebox for radiopharmaceutical production was disposed by using fogging and fixing process. The aerosol ¹³¹I concentrations were detected and the results were analyzed. Results After a 120 minutes fixing, the ¹³¹I contamination in this glovebox reduced from(289 ± 9) DAC and (304 ± 6) DAC to (21.7 ± 2.0) DAC and (26.2 ± 1.8) DAC. After a 180 minutes fixing, the ¹³¹I contamination in this glovebox reduced from (259 ± 10) DAC to (1.80 ± 0.18) DAC. These results showed that no aerosol ¹³¹I contamination was raised again after 24-hours finishing this task. Conclusion Aerosol ¹³¹I concentration in a limited space could be controlled by using a fogging and fixing process, which could reduce the risk of internal exposure of staff. This process could be used by radiopharmaceutical production as an emergency management for dispose high-concentration radioactive aerosol ¹³¹I contamination.