Objective: Our aims were to measure DNA content in primary lung cancer and to study the relationship between the DNA content and TNM stage, histological differentiation of tumor cell, cellular proliferation, and apoptosis. Methods: The DNA content and cellular proliferation were analyzed using flow cytometry. Tumor cell apoptosis was detected by using TUNEL method. Results: (1) The DNA index (DI) distribution ranged from 0.829 to 2.514. There were 41 cases (77.4%) of DNA aneuploid. The distribution of DI and DNA aneuploid was independent of histological subtypes（P>0.05）.(2) With the increase of TNM stage, the DI and the rate of DNA aneuploid increased（P<0.05）.(3) There was relationship between DI and histological differentiation of tumor cell. The DI was higher in tumors of poor differentiation than those in tumors of moderate and good differentiation（P<0.05 and P<0.01）. (4) The cellular proliferation index of aneuploid tumors was significantly higher than that of diploid tumors(P<0.01), while apoptosis index of aneuploid tumors was significantly lower than that of diploid tumors (P<0.01). Conclusion: Correlations exist between DNA content and TNM stage, hiological differentiation, cellular proliferation, and apoptosis.

Objective To investigate the analgesic and anti-inflammatory effects of diuretic mixture and its bacteriostasis effect in vitro,and to provide scientific basis for clinical application.Methods Ear swelling test induced by xylene,twisting reaction test induced by acetic acid,capillary permeability increase in abdominal cavity of mice induced by acetic acid,and pain test induced by formalin were used to observe the analgesic and anti-inflammatory effects of diuretic mixture at high,middle and low doses (crude drug 18.0,9.0,and 4.5 g/kg).Bacteriostatic activities of diuretic mixture were tested by K-B paper disc diffusion method.Results Diuretic mixture alleviated ear edema in mouse model at high dose (P ＜ 0.01).Diuretic mixture at high,middle,and low dose could effectively decrease the twisting reaction (P ＜ 0.01),inhibit capillary permeability (P ＜ 0.05 or 0.01),and ease the ache degree of mice induced by formalin in the first phase (P ＜ 0.01),but there was no significant difference in the degree of pain intensity of phase Ⅱ.The inhibitory rates of diuretic mixture on Staphylococcus aureus and Escherichia coli were 95.04％ and 37.44％,respectively.Conclusion Diuretic mixture has significant effects on analgesia and anti-inflammation and against S.aureus and E.coli in vitro.

Objective To explore the potential influence of a high-fat meal on systemic inflammation and fibrinolytic dysfunction in normocholesterolemic patients with essential hypertension(EH).Methods Plasma concentrations of lipids profiles,high-sensitivity C-reactive protein(hsCRP),plasminogen activator inhibitor type 1(PAI-1)and tissue plasminogen activator(t-PA)antigens in fasting state(F)and at 4 hours after a single high-fat meal(P)were measured in 54 EH patients and 30 healthy controls(Con).Results Postprandial triglyceride concentrations increased significantly in both hypertensive patients and healthy controls(P

Background: Disruption of tight junctions between intestinal epithelial cells followed by loss of barrier function is crucial for the pathogenesis and progression of a variety of gastrointestinal disorders.Aims: To investigate the protective effect of ulinastatin on hydrogen peroxide (H2O2)-induced intestinal epithelial barrier disruption.Methods: Model of intestinal epithelial monolayer barrier was established with Caco-2 cells in vitro,and then divided into four groups: blank control group (without any intervention),H2O2 group (500 μmol/L H2O2),low-dose (500 U/mL) and high-dose (3 000 U/mL) ulinastatin groups (ulinastatin + H2O2).Level of malondialdehyde (MDA) and activity of superoxide dismutase (SOD) were detected;transepithelial electrical resistance (TEER) and flux of sodium fluorescein were measured to assess the barrier function;expression and localization of two tight junction proteins,ZO-1 and occludin were evaluated by Western blotting and immunofluorescence;ultrastructure of tight junctions was observed by transmission electron microscopy (TEM).Results: Compared with the blank control group,treatment of Caco-2 cell monolayers with H2O2 resulted in increase in level of MDA,flux of sodium fluorescein and decrease in activity of SOD,TEER and expressions of ZO-1 and occludin (P all ＜0.05).TEM and immunofluorescence showed that the brusher border of Caco-2 cells in H2O2 group was destroyed,the cell-cell junction was vague and the localization of ZO-1 and occludin was discontinuous and the fluorescence intensity was extremely low.While in ulinastatin groups,especially the high-dose group,all the indices above-mentioned were significantly improved (P all ＜0.05).Conclusions: Ulinastatin protects intestinal epithelial monolayer barrier against H2O2-induced disruption at least partially by its antioxidant activity and modulating expression and localization of tight junction proteins.

OBJECTIVETo investigate gene diagnosis of occult micrometastasis in the mediastinal lymph node in patients with non-small cell lung carcinoma (NSCLC) and to evaluate its prognostic significance.

METHODSWith mRNA expression of mucoid1 (MUC1) gene examined by RT-PCR, 168 mediastinal lymph nodes taken from 37 pN(0) (negative lymph nodes) NSCLC patients (stage Ia approximately IIb) made up the experiment group. Thrity negative lymph nodes from 14 benign lesions and 30 positive lymph nodes from 15 NSCLC patients served as control. The survival difference between MUC1 mRNA-negative and MUC1 mRNA-positive groups was compared by the chi(2) test.

RESULTSUC1 mRNA was not identified in the negative-control group (specificity = 100%), but it was identified in 26 of 30 positive-control samples (sensitivity = 86.7%). MUC1 mRNA was identified in 16 (9.5%) of the experiment group from 12 patients whose TNM stage was up-regulated to stage IIIa. The 3-year survival rate (58.3%) of MUC1 mRNA positive group patients with occult micrometastasis in mediastinal lymph node was lower than the 88.0% of MUC1 mRNA negative group (P < 0.05).

CONCLUSIONOccult micrometastasis in the mediastinal lymph node in NSCLC patients can be diagnosed by MUC1 mRNA expression through RT-PCR. Poor prognosis in some pN(0) NSCLC patients may be associated with nodal occult micrometastasis.

Adult ; Aged ; Carcinoma, Non-Small-Cell Lung ; diagnosis ; secondary ; Female ; Genetic Markers ; genetics ; Humans ; Lung Neoplasms ; diagnosis ; pathology ; Lymphatic Metastasis ; diagnosis ; Male ; Middle Aged ; Mucin-1 ; analysis ; genetics ; Prognosis ; RNA, Messenger ; analysis

This study was aimed to investigate the research on pharmacological effects, relevant ideas and methods of Guangdong native heat-clearing and toxin-removing herbs. Literatures on pharmacological effects of Guangdong native heat-clearing and toxin-removing herbs were collected, summarized and analyzed. The results showed that most Guangdong native heat-clearing and toxin-removing herbs had obvious effects of anti-microbial, anti-bacterial endotoxin, anti-inflammatory, and antipyretic effect. It also had obvious antivenomous and analgesia effect. Based on research ideas and methods of Guangdong native heat-clearing and toxin-removing herbs, we clarified the confusion species as soon as possible, and adhered to the whole animal experiments in combination with in vitro experiments and computer molecular docking simulations for the complementation of each other. It was concluded that the study on pharmacological effects of Guangdongnative heat-clearing and toxin-removing herbs provided strong support in its clinical application. Ideas and methods provided in this article was the main way to explain pharmacological effects of Guangdong native heat-clearing and toxin-removing herbs.

Objective To evaluate the diagnosis and management of bronchial rupture from blunt thoracic trauma. Methods A group of 31 patients with bronchial rupture was involved. Chest roentgenography, tornography and bronchoscopy were performed on all patients. The surgical technique and complications were described.Results Diagnosis was confirmed by tomography and bronchoscopy in all the patients. End to end anastomosis was used in 26 patients. Four patients were operated with total pneurnonectomy. One patient was repaired with an intercostal muscle and rib flap with blood supply. Of the 31 patients, one died of adult respiratory distress syndrome after operation. Most patients had excellent surgical outcomes. 81％ (25/31)of the bronchial rupture were delayed in diagnosis and treatment. The classic symptoms and signs of bronchial rupture included subcutaneous emphysema, dyspnea and an intermediate coma interval. The roentgenogram showed mediastinal emphysema, pneumothorax, “drop lung” sign and marked radiodensity of hilum widened mediastinum.Conclusion Bronchoscopy is a useful and accurate method to diagnose and treat the bronchial rupture, with which surgeons can easily locate the rupture site during surgery. Surgical treatment could restore pulmonary function in most patients.

Objectives To investigate the frequency, distribution and features of lymph nodes metastasis in T1/T2 squamous carcinoma and adenocarcinoma of lung, and to provide evidence for extensive dissection of lymph nodes．Methods 254 patients with T1/T2 squamous caroinoma and adenocarcinoma of lung underwent R2 surgery plus extensive dissection of hilar, interlobular and mediastinal lymph nodes according to the grouping system proposed by Naruke．Results A total of 1685 groups of lymph nodes were dissected． The metastatic rates of N1 and N2 were 20．0% and 10．2%． The differerce was very significant between T1 and T2 (P<0．01)． No, N2 metastasis was found in T1 squamous carcinoma． N2 metastatic rates were 22．0% in squamous carcinoma and 40．9% in adenocarcinoma (P<0．01)． 64．3% of squamous carcinomas spread to only one group of N2 nodes,and over 3 groups of lymph nodes were positive in 46．2% of adenocarcinonmas． Saltatory metastasis accounted for 57．5% of N2 metastasis． 13．6% of N2-positive tumors in the upper lobes metastasized to the lower mediastinum, whereas 51．6% of N2-positive tumors in the lower lobes spread to the upper mediastinum．Conclusions The frequency of lymph node metastasis increases with the growth of tumors． Metastasis occurs more frequently in adenocarcinoma than in squamous carcinoma． Tumor at any site can metastasize to the distant mediastinum． Except for T1 squamous carcinoma, radical surgery can be achieved only by extensive dissection of ipsilateral intrapulmonary and mediastinal lymph nodes．

Objectives To investigate the frequency, distribution and features of lymph nodes metastasis in T1/T2 squamous carcinoma and adenocarcinoma of lung, and to provide evidence for extensive dissection of lymph nodes．Methods 254 patients with T1/T2 squamous caroinoma and adenocarcinoma of lung underwent R2 surgery plus extensive dissection of hilar, interlobular and mediastinal lymph nodes according to the grouping system proposed by Naruke．Results A total of 1685 groups of lymph nodes were dissected． The metastatic rates of N1 and N2 were 20．0% and 10．2%． The differerce was very significant between T1 and T2 (P<0．01)． No, N2 metastasis was found in T1 squamous carcinoma． N2 metastatic rates were 22．0% in squamous carcinoma and 40．9% in adenocarcinoma (P<0．01)． 64．3% of squamous carcinomas spread to only one group of N2 nodes,and over 3 groups of lymph nodes were positive in 46．2% of adenocarcinonmas． Saltatory metastasis accounted for 57．5% of N2 metastasis． 13．6% of N2-positive tumors in the upper lobes metastasized to the lower mediastinum, whereas 51．6% of N2-positive tumors in the lower lobes spread to the upper mediastinum．Conclusions The frequency of lymph node metastasis increases with the growth of tumors． Metastasis occurs more frequently in adenocarcinoma than in squamous carcinoma． Tumor at any site can metastasize to the distant mediastinum． Except for T1 squamous carcinoma, radical surgery can be achieved only by extensive dissection of ipsilateral intrapulmonary and mediastinal lymph nodes．

Dendrobium officinale is a Chinese herbal medicine with the same origin as medicine and food,and it is commonly used in making soup and tonic diet,etc.Dendrobium officinale and its compound preparations are widely used in clinical practice.This article used keywords such as"Dendrobium officinale"and"clinical"to search for relevant literature on clinical research on Dendrobium officinale in CNKI,Wanfang,VIP,PubMed and other databases over the past 10 years.It is found that Dendrobium officinale and its compound preparations were mainly used in the treatment of internal medicine(such as chronic gastritis,diabetes,bronchitis,hypertension,coronary heart disease,etc.),surgery,gynecology,pediatrics,ophthalmology and other diseases,and had good clinical effects.It is a Chinese herbal medicine with the development value.This article provides a comprehensive and systematic summary and analysis of the clinical application of Dendrobium officinale,aiming to provide reference for its rational clinical application and further development and research.