OBJECTIVETo construct strains containing green fluorescent protein (GFP) to study gene regulation in Saccharomyces albicans cells during the infection process.
METHODSpACT1-GFP was constructed, and Saccharomyces albicans CAI4 was transformed. The expression of GFP in yeast and hyphal compartments was observed with microscopy.
RESULTS99% of Saccharomyces albicans cells containing pACT1-GFP fusion displayed significant fluorescence levels both in the yeast and hyphal compartments. The fluorescence intensity in two compartments had no obvious difference.
CONCLUSIONpACT1-GFP can be expressed stably in the yeast cells.
Candida albicans
;
Green Fluorescent Proteins
;
Humans
;
Plasmids
;
Saccharomyces
;
Saccharomyces cerevisiae