1.The construction and expression of Saccharomyces albicans pACT1-GFP plasmids.
Jing SUN ; Fen JIA ; Ming-Hui XIA ; Hua QIAN ; Hongnan DONG ; Qingguo QI
West China Journal of Stomatology 2012;30(2):115-118
OBJECTIVETo construct strains containing green fluorescent protein (GFP) to study gene regulation in Saccharomyces albicans cells during the infection process.
METHODSpACT1-GFP was constructed, and Saccharomyces albicans CAI4 was transformed. The expression of GFP in yeast and hyphal compartments was observed with microscopy.
RESULTS99% of Saccharomyces albicans cells containing pACT1-GFP fusion displayed significant fluorescence levels both in the yeast and hyphal compartments. The fluorescence intensity in two compartments had no obvious difference.
CONCLUSIONpACT1-GFP can be expressed stably in the yeast cells.
Candida albicans ; Green Fluorescent Proteins ; Humans ; Plasmids ; Saccharomyces ; Saccharomyces cerevisiae
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