1.Clinical Research of CO2 Laser Resection of the Paraglottic Space and Arytenoid for the Treatment of Bilateral Vocal Cord Paralysis
Chinese Journal of Minimally Invasive Surgery 2016;16(7):645-647
[Summary] This paper reported 10 patients with bilateral vocal cord paralysis from March 2013 to October 2015.The paraglottic space and arytenoid were resected with CO 2 laser.The endotracheal intubation was removed at 3 months after surgery .The patients were followed up for 1-2 years.No dyspnea or eating difficulty was seen .Patient’ s voice was normal.The cavity mucous membrane was smooth .No complications such as granulation tissue growth occurred .
2.Advances in Research on Dendritic Cell-based Tumor Vaccine
Hongmin LU ; Linfeng LI ; Jianxin GAO
Chinese Journal of Gastroenterology 2016;21(5):257-262
Although tumor immunotherapy has been proposed for many years,the consensus denoting it as an essential approach for fighting against cancer is reached only in recent years. Tumor immunotherapy can be categorized as active and passive ones. In order to successfully cure cancer,safe and efficient active immunotherapy is required. Dendritic cells (DCs)are not only the bridge linking innate and adaptive immunity,but also the key determinants of the quality of adaptive immunity:immunity versus immune tolerance. Therefore,the safe and efficient DC-based tumor-specific and broad-spectral tumor vaccine has an irreplaceable important position in tumor immunotherapy. Because of the high heterogeneity of DCs, the research on DC-based tumor vaccine has encountered a bottleneck. Here,we reviewed the progress in research on DC-based tumor vaccine and related problems needed to be resolved with the incorporation of our experiences.
3.The studies on therapeutic action of tuberculosis Ag85A DNA vaccines
Xueqiong WU ; Junxian ZHANG ; Hongmin LI
Chinese Journal of Immunology 1985;0(01):-
To study the therapeutic action of tuberculosis Ag85A DNA vaccines. Methods:BALB/c mice were infected by in-traperitoneal injection with M. tuberculosis H37Rv for 8 weeks, and then treated with the saline (A), plasmid vector (B), M. bovis BCG (C), M. vaccae (D), and Ag85A DNA vaccine (E). Ag85A-specific antibodies were determined by ELISA. The lungs, livers and spleens were taken and observed their pathological changes, weighted and performed mycobacterial cultures 2 or 5 monthes after treatment. Spleen cells were tested for proliferative responses.Results:Ag85A-specific antibodies increased in the mice of group E. At 2 months after immunothera-pies, the stimulation rates of spleen cells had no significant difference among each group. The numbers of viable bacteria in the lungs and spleens of therapeutic group were lower than those in the control group. The group C and B could be observed slight .lesion of lung. At 5 months after immunotherapies, the stimulation rates of spleen cells all increased significantly in the immunotherapeutic groups. The numbers of viable bacteria in the lung and spleen had no significant difference among each group. No lesions of lung could be observed in group E and D. Conclusion:The tuberculosis DNA vaccines seem to have some immunotherapeutic actions.
4.embB mutations in Mycobacterium tuberculosis ethambutol-resistant isolates
Xueqiong WU ; Jianqin LIANG ; Hongmin LI
Chinese Journal of Laboratory Medicine 2001;0(04):-
Objectives To understand the mutations of embB genes in M. tuberculosis isolates, and to evaluate their clinical value. Method 102 clinical isolates were identified for their mycobacterial species, and then analyzed their embB genes with PCR SSCP, PCR RFLP, and PCR direct sequencing. Results Mycobacterium tuberculosis strain H 37 R v was used as a control. 102 clinical isolates all had the same 16S rDNA SSCP profiles as M. tuberculosis . Forty one drug sensitive isolates had normal embB SSCP and RFLP profiles. Of 61 ethambutol resistant isolates, 23 (37.7%) displayed abnormal embB SSCP profiles. Eight isolates had abnormal RFLP profiles. All embB mutations situated at codon 306, whose EMB MICs were more than 20 ?g/ml. Eight isolates had ATG to ATA or ATT mutations at codon 306. Thirty isolates had ATG to GTG or CTG mutations at codon 306, whose EMB MICs were more than 30 ?g/ml. Conclusions Ethabutol resistances in some M. tuberculosis isolates were due to mutations on embB genes. PCR SSCP and PCR RFLP method might become a simple and rapid diagnostic test for genotypes of M. tuberculosis ethabutol resistance.
5.Long-term follow-up of inferior vena cava filters in conjunction with thrombolysis and anti-coagulant therapy in prevention of pulmonary embolism in patients with deep vein thrombosis
Hongbing YAN ; Hong LI ; Hongmin SONG
Chinese Journal of Interventional Cardiology 1993;0(02):-
Objective To analyze retrospectively long term follow up of inferior vena cava filters in conjunction with thrombolysis and anti coagulant therapy in prevention and treatment of pulmonary embolism (PE) in 24 patients with deep vein thrombosis (DVT) Methods This study included 13 males and 11 females with an average age of 52 4 years (14-86 years) Percutaneous implantation of inferior vena cava filters was performed via a femoral vein in 24 patients with acute or subacute DVT, of whom two were given conjunctive catheter based urokinase thrombolysis After the procedure, 20 patients underwent intravenous urokinase thrombolysis with subcutaneous low weight molecular heparin for 10 days and subsequent oral warfarin for six months Results All patients underwent a successful interventional procedure with an average 15 month (10-48 months) follow up One week after the procedure, relief of symptoms related to DVT was achieved in all the 24 patients Neither filter migration and thrombolic occlusion of filter nor PE and major hemorrhage were observed in this series Conclusion The use of inferior vena cava filters in conjunction with thrombolysis and anti coagulant therapy is a safe and effective treatment modality in patients with DVT, which can be used to prevent subsequent PE
6.CLINICAL APPLICATION AND EVALUATION OF DETECTION OF THREE DRUG RESISTANCE GENES OF MYCOBACTERIUM TUBERCULOSIS
Wei WANG ; Hongmin LI ; Xueqion WU
Medical Journal of Chinese People's Liberation Army 1982;0(03):-
To evaluate the clinical value of PCR SSCP to detect the rPOB, katG and rPSL resistance genes in M. tuberculosis, the mutation of genes and results of drug sensitive test in strains of M. tuberculosis isolated from 123 patients and grown with Bactec 960 were analyzed, drug sensitive test and polymerase chain reaction single stranded conformation polymorphism (PCR SSCP) were performed. Clinical therapeutic effect was evaluated. In nearly one third of the patients, resistance against one drug was found in primary treatmnent. The mutation rate of RFP, INH and SM drug sensitive isolates was 6 2%, 10 8%, and 25 4%, respectively, while that of drug resistant isolates was 60 2%, 48 0%, and 61 5%, respectively. The magnitude of drug resistance was in accordance with the increase in mutation rate of genes. Detection of the resistance genes could be a new approach in guiding treatment, and it might complement drug sensitive test in clinical management of tuberculosis patients.
7.THE RESEARCH OF FIVE DRUG-RESISTANCE GENES OF MYCOBACTERIUM TUBERCULOSIS IN 134 PATIENTS
Hongmin LI ; Xueqiong WU ; We WANG
Medical Journal of Chinese People's Liberation Army 2001;0(11):-
M.tuberculosis strains isolated strains from sputum specimens of 134 patients were analyzed by PCR SSCP and traditional drug susceptibility tests. The results sbowed that the gene mutation rate of PZA, SM, RFP,INH and EMB resistance in those clinically isolated strains was 42 7%,71 7%,78 9%,68 6% and 43 9%, respectively. The gene mutation was in relation with drug resistance level of M. tuberculosis. The gene mutation rate was higher in high concentration resistance strains than in low concentration resistance strains.
8.MEASUREMENT AND EVALUATION OF SERUM SEX HORMONES IN PATIENTS WITH SEVERE ACUTE RESPIRATORY SYNDROME (SARS)
Wei WANG ; Yixiu YE ; Hongmin LI
Medical Journal of Chinese People's Liberation Army 2001;0(09):-
Objective To observe the levels of serum sex hormone in patients with severe acute respiratory syndrome (SARS), in order to determine whether there were involvement of sex glands. Methods The levels of serum E2, PROG, FSH, LH and PRL were measured in 66 patients with severe acute respiratory syndrome (SARS) by electrochemiluminescence, and the results were compared with that of controls. Results The results showed that levels of serum E2, PROG,and FSH in patients with SARS were significantly lower than those of controls. On the other hand, the levels of serum LH and PRL were significantly higher than those of controls. The level of E2 in patients with severe type SARS was significantly lower than that in patients with mild type.The levels of sex hormones returned later than improvement of clinical symptoms. Conclusion The levels of serum sex hormones were lowered in patients with SARS, indicating that the changes in sex hormones might play a role in the disease process of SARS. Recovery of levels of sex hormones was retarded.
9.The Mutation of embB and pncA Genes in Drug-resistanct Mycobacterium Tuberculosis and Its Clinical Value
Hongmin LI ; Xueqiong WU ; Wei WANG
Journal of Chinese Physician 2001;0(06):-
Objective To investigate the mutation of embB and pncA genes in mycobacterium tuberculosis isolates, and assess its clinical value. Methods The drug-sensitivity of 106 clinical isolates of mycobacterial species was identified by the tranditional method, and then analyzed the mutation of their embB and pncA genes with PCR-SSCP. Mycobacterium tuberculosis strain H 37 RV was used as a control. Results The mutational frequency of pncA gene in 94 PZA-resistant mycobacterium tuberculosis was 44 6%(42/94). The mutational frequency of embB gene in 83 EMB-resistant isolates was 54 2%. The frequency of both genes mutation was 11 8%(11/94). Conclusion EMB and PZA resistances in some mycobacterium tuberculosis isolates were due to mutations of embB and pncA genes. PCR- SSCP method might become a simple and rapid diagnostic test for genotypes of EMB and PZA resistant mycobacterium tuberculosis.
10.The Level Change of Serum Superoxide Dismutase in Patients with Severe Acute Respiratory Syndrome
Yixiu YE ; Wei WANG ; Hongmin LI
Journal of Chinese Physician 2001;0(01):-
Objective To observe the level change of serum SOD in patients with severe acute respiratory syndrome (SARS). Methods The levels of serum SOD in 66 patients with SARS were measured by RIA, and compared with controls. Results The levels of serum SOD in patients with SARS were significantly lower than those in controls, and in severe type of SARS patients were lower than those in the other types. The levels of serum SOD in the recovery stage of SARS patients increased, but still was lower than those in controls. Conclusion Excessive free radicals were produced in patients with SARS, and SOD was depleted. The level change of SOD in patients with SARS may reflect state of disease. Dynamic detection of serum SOD level is helpful for monitoring state of SARS. It may be an important therapeutic measure that excessive free radicals were eliminated from SARS patient body.