[Summary] This paper reported 10 patients with bilateral vocal cord paralysis from March 2013 to October 2015.The paraglottic space and arytenoid were resected with CO 2 laser.The endotracheal intubation was removed at 3 months after surgery .The patients were followed up for 1-2 years.No dyspnea or eating difficulty was seen .Patient’ s voice was normal.The cavity mucous membrane was smooth .No complications such as granulation tissue growth occurred .

To evaluate the clinical value of PCR SSCP to detect the rPOB, katG and rPSL resistance genes in M. tuberculosis, the mutation of genes and results of drug sensitive test in strains of M. tuberculosis isolated from 123 patients and grown with Bactec 960 were analyzed, drug sensitive test and polymerase chain reaction single stranded conformation polymorphism (PCR SSCP) were performed. Clinical therapeutic effect was evaluated. In nearly one third of the patients, resistance against one drug was found in primary treatmnent. The mutation rate of RFP, INH and SM drug sensitive isolates was 6 2%, 10 8%, and 25 4%, respectively, while that of drug resistant isolates was 60 2%, 48 0%, and 61 5%, respectively. The magnitude of drug resistance was in accordance with the increase in mutation rate of genes. Detection of the resistance genes could be a new approach in guiding treatment, and it might complement drug sensitive test in clinical management of tuberculosis patients.

M.tuberculosis strains isolated strains from sputum specimens of 134 patients were analyzed by PCR SSCP and traditional drug susceptibility tests. The results sbowed that the gene mutation rate of PZA, SM, RFP,INH and EMB resistance in those clinically isolated strains was 42 7%,71 7%,78 9%,68 6% and 43 9%, respectively. The gene mutation was in relation with drug resistance level of M. tuberculosis. The gene mutation rate was higher in high concentration resistance strains than in low concentration resistance strains.

Objective To observe the levels of serum sex hormone in patients with severe acute respiratory syndrome (SARS), in order to determine whether there were involvement of sex glands. Methods The levels of serum E2, PROG, FSH, LH and PRL were measured in 66 patients with severe acute respiratory syndrome (SARS) by electrochemiluminescence, and the results were compared with that of controls. Results The results showed that levels of serum E2, PROG,and FSH in patients with SARS were significantly lower than those of controls. On the other hand, the levels of serum LH and PRL were significantly higher than those of controls. The level of E2 in patients with severe type SARS was significantly lower than that in patients with mild type.The levels of sex hormones returned later than improvement of clinical symptoms. Conclusion The levels of serum sex hormones were lowered in patients with SARS, indicating that the changes in sex hormones might play a role in the disease process of SARS. Recovery of levels of sex hormones was retarded.

Objective To construct lentivirus vectors carrying alphastatin gene, test its secretion expression in human umbilical vein endothelia cells (HUVECs) and observe its effects on growth, migration and tube formation of HUVECs. Methods We constructed recombinant lentivirus vectors of NT4-alphastatin fusion gene containing neurotrophin-4 signal peptide, pro-region sequences and alphastatin, then transfected the recombinant lentivirus vectors into HUVECs to obtain secretory protein alphastatin and test its anti-angiogenic activities in vitro. Results Our data showed that recombinant self-inactivating lentivirus vectors of NT4-alphastatin were successfully constructed, and stable NT4-alphastatin transduced HUVECs were capable of sustainably secreting alphastatin which significantly suppressed HUVECs migration and differentiation but not VEGF-induced proliferation. Conclusion This report represents the first time on the use of lentivirus-based vectors to deliver alphastatin, the endogenous angiogenesis inhibitor, and reveals the potential utility of anti-angiogenic gene therapy with lentivirus vectors for treating cancer.

To study the therapeutic action of tuberculosis Ag85A DNA vaccines. Methods:BALB/c mice were infected by in-traperitoneal injection with M. tuberculosis H37Rv for 8 weeks, and then treated with the saline (A), plasmid vector (B), M. bovis BCG (C), M. vaccae (D), and Ag85A DNA vaccine (E). Ag85A-specific antibodies were determined by ELISA. The lungs, livers and spleens were taken and observed their pathological changes, weighted and performed mycobacterial cultures 2 or 5 monthes after treatment. Spleen cells were tested for proliferative responses.Results:Ag85A-specific antibodies increased in the mice of group E. At 2 months after immunothera-pies, the stimulation rates of spleen cells had no significant difference among each group. The numbers of viable bacteria in the lungs and spleens of therapeutic group were lower than those in the control group. The group C and B could be observed slight .lesion of lung. At 5 months after immunotherapies, the stimulation rates of spleen cells all increased significantly in the immunotherapeutic groups. The numbers of viable bacteria in the lung and spleen had no significant difference among each group. No lesions of lung could be observed in group E and D. Conclusion:The tuberculosis DNA vaccines seem to have some immunotherapeutic actions.

Although tumor immunotherapy has been proposed for many years,the consensus denoting it as an essential approach for fighting against cancer is reached only in recent years. Tumor immunotherapy can be categorized as active and passive ones. In order to successfully cure cancer,safe and efficient active immunotherapy is required. Dendritic cells (DCs)are not only the bridge linking innate and adaptive immunity,but also the key determinants of the quality of adaptive immunity:immunity versus immune tolerance. Therefore,the safe and efficient DC-based tumor-specific and broad-spectral tumor vaccine has an irreplaceable important position in tumor immunotherapy. Because of the high heterogeneity of DCs, the research on DC-based tumor vaccine has encountered a bottleneck. Here,we reviewed the progress in research on DC-based tumor vaccine and related problems needed to be resolved with the incorporation of our experiences.

Objective To evaluate the clinic application value of detection of rPOB,katG and rPSL resistance genes to M tuberculosis by polymerase china reaction-single stranded conformation polymorphism (PCR-SSCP).Method The drug-resistance tests of M tuberculosis clinical isolates from 141 patients with the tuberculosis was analyzed by Bactec-960 rapid culture,drug-sensitive and PCR-SSCP techniques.Results As nearly As one of three patients with priliminary treatment had resistance to one kind of the drugs tested at least.The mutation rates of RFP,INH and SM drug-sensitive isolates were 6 5%,11 4% and 27 2% respectively.That of the drug-resistant isolates were 60 2%,45 7% and 62 7%.As drug-resistant concentration heightened,so mutation rate of genes increased.Conclusions Detecting the resistance genes was a new explore in guiding treatment,and multiple drug-sensitive test combinative use have cooperativity each other.It might become good test for clinical management.

Objective To investigate the mutation of embB and pncA genes in mycobacterium tuberculosis isolates, and assess its clinical value. Methods The drug-sensitivity of 106 clinical isolates of mycobacterial species was identified by the tranditional method, and then analyzed the mutation of their embB and pncA genes with PCR-SSCP. Mycobacterium tuberculosis strain H 37 RV was used as a control. Results The mutational frequency of pncA gene in 94 PZA-resistant mycobacterium tuberculosis was 44 6%(42/94). The mutational frequency of embB gene in 83 EMB-resistant isolates was 54 2%. The frequency of both genes mutation was 11 8%(11/94). Conclusion EMB and PZA resistances in some mycobacterium tuberculosis isolates were due to mutations of embB and pncA genes. PCR- SSCP method might become a simple and rapid diagnostic test for genotypes of EMB and PZA resistant mycobacterium tuberculosis.

Objective To observe the level change of serum SOD in patients with severe acute respiratory syndrome (SARS). Methods The levels of serum SOD in 66 patients with SARS were measured by RIA, and compared with controls. Results The levels of serum SOD in patients with SARS were significantly lower than those in controls, and in severe type of SARS patients were lower than those in the other types. The levels of serum SOD in the recovery stage of SARS patients increased, but still was lower than those in controls. Conclusion Excessive free radicals were produced in patients with SARS, and SOD was depleted. The level change of SOD in patients with SARS may reflect state of disease. Dynamic detection of serum SOD level is helpful for monitoring state of SARS. It may be an important therapeutic measure that excessive free radicals were eliminated from SARS patient body.