Objective To study on the effect of situational teaching based on ECS in medical nursing teaching.Methods 93 nursing students were divided into the experimental group (45 cases) and the control group (48 cases).The experimental group adopted ECS in the medical nursing situational teaching,while the control group used conventional teaching method.The teaching results between the two groups were compared between two groups.Results Two groups had significant differences in the medical nursing theory test and the operation skills examination.The students in the experimental group had higher recognition on the situational teaching based on ECS,especially in the learning initiative and interest,pathology observation ability and the unity cooperation ability,the differences showed statistical difference.Conclusions The application of ECS in the medical nursing situational teaching can stimulate the learning interest of the nursing students,help the cultivation of clinical thinking ability,relieve the contradiction of resources strain in nurses clinical probation and promoting the improvement of medical nursing teaching.

Objective To investigate the effects of hyaluronan (HA) and CD44 on airway mucous hypersecretion,and explore the molecular mechanism of activation of epidermal growth factor(EGF)/epidermal growth factor receptor(EGFR) signal transduction pathway by signal factors. Methods BEAS-2 B airway epithelial cells were cultured in vitro,and were stimulated by neutrophil elastase(NE).Reactive oxygen species(ROS)scavenger(DMTU),hyaluronidase(Hase),CD44 antibody and tissue kallikrein(TK)inhibitor(PI)were served as interventional factors,and control group(serum free culture),NE stimulation group(50 nmol/L NE),DMTU+NE group(20μmol/L DMTU+50 nmol/L NE),DMTU+Hase+NE group(20 μmol/L DMTU+10μg/mL Hase+50 nmol/L NE),CD44Ab+NE group(5 μg/mL CD44Ab+50 nmol/LNE)and PI+NE group(1 00μg/mL PI+50 nmol/L NE)were established.The expression of mucin(MUC)5AC mRNA was detected by RT-PCR,the expression of MUC5AC and EGF protein was determined by ELISA,and the expression of phosphorylated epidermal growth factor receptor(p-EGFR)protein was analysed by Western blotting. Results The expression of MUC5AC,EGF and p-EGFR protein and MUC5AC mRNA in NE stimulation group was significantly higher than that in control group(P<0.01),the expression in DMTU+NE group was significantly lower than that in NE stimulation group(P<0.01),the expression in MTU+Hase+NE group was significantly higher than that in DMTU+NE group(P<0.05 for MUC5AC and p-EGFR protein and MUC5AC mRNA,and P<0.01 for EGF protein),the expression in CD44Ab+NE group and PI+NE group was significantly higher than that in NE stimulation group(P<0.05 for MUC5AC and p-EGFR protein and MUC5AC mRNA,and P<0.01 for EGF protein). Conclusion NE up-regulates the expression of MUC5AC gene via ROS/HA/CD44/TK/EGF/EGFR signal transduction pathway in airway epithelial cells.

Retrovirus (RV), a major pathogen in acute gastroenteritis in children that may lead to severe disease and heavy burdens on national economy. There is no speciifc drug treatment for the gastroenteritis caused by RV, and vaccine has been identiifed as the most effective intervention to control the associated disease burden. The RV vaccine showed differential effects on different RV infection. In China, epidemiology of RV is more complex hence demands more epidemiologic studies. In this article, we will review the epidemiology of RV in China and further discuss the importance of generalized RV vaccine.

Objective To explore the issues of everyday expenses list used in the cadre wards and find out countermeasures for the purpose of reducing medical disputes and improving the patients’satisfaction.Method Seventy-four patients admitted in our department from July to September 2012 were set as control group,where an investigation was conducted to find out the issues in the practical use of every expenses list and corresponding measures were worked out and used in observation group,which included 78 patients admitted in the department from October to December 2012.The two groups were compared in terms of self-paid articles, re-recording of missed expenses,failure to timely refunding,charging by other means,disagreement on curative nursing items and unclear items for charging.Result The observation group was significantly better than the control in self-paid articles,re-recording of missed expenses and failure to timely refunding(all P<0.05).Conclusions There are some issues in the use of everyday expenses list in the cadre wards.We should take corresponding countermeasures to ensure the interests of the hospital and the patients and the enhanced degree of patients’comfort.

Objective:To investigate the effects of fluvastatin on high-sensitivity c-reactive protein( hs-CRP) , tumor necrosis fac-tor-α(TNF-α), urine albumer excretion rate (UAER)and serum creatinine (Cr) in the patients with early diabetic nephropathy. Methods:Totally 69 patients with early diabetic nephropathy were randomly divided into the observation group with 34 cases and the control group with 35 cases. The observation group was treated by low-protein diet plus fluvastatin, and the control group was treated by low-protein diet only. The course of treatment was 8 weeks. Totally 65 nondiabetic persons were selected as the normal group, and the levels of TNF-α, hs-CRP, UAER, ALT and Cr were detected before and after the treatment. Results:The levels of TNF-α, hs-CRP and UAER in the observation group and the control group were significantly higher than those in the normal group(P<0. 05);the lev-els of ALT and Cr showed no significant differences (P>0. 05). Compared with that before the treatment, the level of UAER was de-creased significantly in the control group after the treatment (P<0. 05), and the levels of TNF-α and hs-CRP showed no significant differences after the treatment (P>0. 05). In the observation group, the levels of TNF-α, hs-CRP and UAER were all decreased sig-nificantly after the treatment (P<0. 05). Conclusion:UAER, hs-CRP and TNF-α are closely connected with diabetic nephropathy. Fluvastatin can decrease the levels of TNF-α, hs-CRP and UAER. Low-protein diet plus fluvastatin is effective and safe in the treat-ment of early diabetic nephropathy, and the efficacy is superior to that of low-protein diet only.

Objective To investigate the diagnosis under hysteroscopy for uterine malformation,especially in maiden. Methods Hysteroscopy combined with ultrasonography was used to determine the types of uterine malformation in 24 cases. Results Definitive diagnosis indicated 8 cases of uterus bicornis,10 cases of uterus septus with single uterine neck,2 cases of uterus septus with double uterine neck and single uterine body,and 4 cases of puberal rudimentary horn of uterus. Conclusions Combined use of hysteroscopy and ultrasonography in the diagnosis of uterine malformation is accurate and applicable to maiden.

Aim To investigate the effects of Curcumin on the APP processing in the amyloidogenic pathway in vitro and explore its mechanisms on A? generation inhibition.Methods Plasmids APPswe and BACE1-mychis were transiently co-transfected in SH-SY5Y cell by LipofectaminTM2000.The cell line then treated with Curcumin at 0,1.25,5,20 ?mol?L-1 for 24 h,or with Curcumin at 5 ?mol?L-1 for 0,12,24 and 48 h for the time course assay.RT-PCR were performed to measure the endogenous levels of APP and BACE1 mRNA.Western blot were used to detect the protein expression of BACE1 and C99,the major ?-secretase cleavage product.The concentration of A?40/42 was detected by ?-amyloid 1~40 or 42 Colorimetric ELISA.Results RT-PCR results showed that the mRNA levels of APP and BACE1 were decreased obviously in a dose-and time-dependent manner after treated with Curcumin in SH-SY5Y(P

With the development of systems biology and high-throughput molecular technologies, systems toxicology has come into being. The up-date understanding of systems toxicology is the study of the perturbation of biological systems by using chemicals and stressors, monitoring changes in molecular expression (transcript, protein and metabolite profiling), conventional toxicological parameters and iteratively integrating response data to describe the functioning organism. The background, research framework, supporting tools and the main applications of systems toxicology were discussed in detail in the present paper. Although in its infancy, systems toxicology will play an important role in advancing the study of environmental stress and heath impact definitely.

Objective:To study the mechanism of 149 serine in the abrogation of cdc25b as a meiosis inducer in mouse oocytes.Methods: Immunofluorescence analysis was used to observe the distribution of cdc25b protein in GV stage and G2/M transition oocytes;Fused plasmids of pEGFP-cdc25b-wt,pEGFP-cdc25b-s149a and vector pEGFP-C3 were microinjected into GV mouse oocytes.After culturing for a certain time,the subcellular localization of protein was observed.Results:cdc25b-wt was distributed mainly in cytoplasm in GV stage oocytes and accumulation of protein was observed during G2/M transition while green fluoresent signal of cdc25b-s149a was observed in nuclear and cytoplasm.Conclusion:The results suggest that there existed a shuffling between nuclear and cytoplasm of cdc25b in GV stage oocytes although they seem to be quilt,the phosphorylation of 149 serine could maintain proper distribution of protein by balancing the speed of protein nuclear export and nuclear import.

Nucleic acid aptamers are ligands with high specificity and affinity for their targets,which are screened from large oligonucleotide pools by the SELEX (systematic evolution of ligands by exponential enrichment,SELEX) technology. For their specific character,they have shown the great applicative prospects in the environment toxicology and related research fields. The recent related researches were reviewed in the present paper.