Cell protection from stresses by heat shock protein (HSP) was previously attributed to its ability to prevent aggregation and to accelerate refolding of damaged proteins. It plays an important role in cell survival after extremely harsh protein damaging treatment leading to necrotic cell death. On the other hand, protein repair function of HSP cannot explain how it protects cell from stresses which do not cause direct protein damage. These stresses kill cell through activation of apoptotic program. Recently it has been found that HSP can meditate suppression of a stress-activated protein kinase, JNK, an early component of stress-induced apoptotic signaling pathway.These observations can provide a basis for HSP's function of anti-apoptosis.

Cell protection from stresses by heat shock protein (HSP) was previously attributed to its ability to prevent aggregation and to accelerate refolding of damaged proteins. It plays an important role in cell survival after extremely harsh protein damaging treatment leading to necrotic cell death. On the other hand, protein repair function of HSP cannot explain how it protects cell from stresses which do not cause direct protein damage. These stresses kill cell through activation of apoptotic program. Recently it has been found that HSP can meditate suppression of a stress-activated protein kinase, JNK, an early component of stress-induced apoptotic signaling pathway.These observations can provide a basis for HSP's function of anti-apoptosis.

OBJECTIVEThis study aimed to evaluate the influence of loading in different healing periods on implant stability and to establish an animal model of anchorage implant screws.

METHODSAnchorage implant screws were implanted in sheep alveolar bone to establish the implant screw animal model. The animals were randomly divided into four groups: group A, sheep without loading; group B, sheep loaded immediately after implant; group C, sheep loaded after two weeks; and group D, sheep loaded after four weeks. The maxillary and mandibular tissue specimens with implants were dissected. The maxillary tissue specimens were used to make undecalcified bone grinding slices, and the healing mode of the implant-bone interface was observed by light microscope. The maximum shear strength of loose mandibular specimens was measured by a material testing machine. The differences in group data were statistically analyzed. RESULTS 1) A general difference in shear strength exists among the four groups. The shear strengths in groups A and B were lower than those in groups C and D. 2) No significant difference in healing mode was found between the pressure side and the tension side of the longitudinal-grinded bone slices with implants. Both fiber combination and osseointegration of transverse-grinded slices could be found in the interface in all four groups. The major integration mode of the implant-bone interface of groups A and D was osseointegration, whereas that of the implant-bone interface of groups B and C was fibrous tissue transformation. The surrounding bone tissue regenerated toward the implants of group C.

CONCLUSIONResults show that loading time influences the stability of implant anchorage.

Objective Clinical efficacies of angiogenesis and improvement in lower limb ischemia are compared for the transplantation of both the peripheral blood stem cells(PBSC)and bone marrow stem cells(BMSC).Methods A total of 170 patients suffering from lower limb ischemia who received stem cell transplantation were monitored for random comparison study during September 2004 to October 2006.Among them 94 patients were treated with PBSC and 76 were BMSC.All the clinical data and laboratory findings before and after the transplantation in the 1st.12th and 24th weeks were comprehensively evaluated and tested by SPSS 12.0 software package.Results The total number of MNC,CD34+ cells and CD133+ cells in PBSC group was greatly more than BMSC group and had prominent or very prominent variances(P

Objective To study the factors related to hyperuricaemia in type 2 diabetic patients and to investigate the effect of serum uric acid levels on the metabolic syndrome.Methods During March 2007 and March 2008 731 cases were divided into 2 groups according to their uric acid levels,then the relationship between serum uric acid and metabolic parameters and the complications in two groups were observed.Results There was a positive correlation between uric acid levels and triglycerides,body mass index,blood pressure(P

Aim To study the effects of D-galactose o n bone of rats. Methods Forty-two Sprague-Dawley rats at age of 3 months with half female and half male were divided into five groups randoml y, basal, OVX or ORX , D-galactose at dose of 50 mg?kg -1?d -1 (L), D-galactose at dose of 100 mg?kg -1?d -1 (M), galactose a t dose of 200 mg?kg -1?d -1 (H).The former two groups were SC inject ed physiological saline and the latter three groups were injected three differen t doses of D-galactose respectively. Two months later the rats were killed by setting blood free from hearts, then the left thighbones and tibias were take n. The tibias were cut at 8 ?m sections for static and dynamic bone histomorph ometric analyses. The thighbones were dried and weighed. All rats were given sc injections of tetracycline 25 mg?kg -1 (first fluorochrome bone marker) on 14 and d 13,and calcein 10 mg?kg -1(second fluorochrome bone marker) on 4 and d 3 before sacrifice. The testicles and the ovaries were taken and made the paraffin slices for observing the microstructure under the microscope. Results D-galactose had no effects on bone of female rats, but can made male rats lose bone significantly and the rats appeared osteoporosis. The static parameters in three different dose male rats bone which relate to bone ma ss %Tb. Ar decreased 53.5%,53.5%,46.6% respectively, Tb.N decreased 42.9%,4 7.6%,38.1% respectively, TB.Sp increased 85%,150%,67.3% respectivly. The dynamic parameters which relate to bone formation %L. Pm decreased 24.1% 89.1% , 65.5% respectively. The convoluted suminiferous tubule disordered, lumens dil ated, and basal membrane disintegrated. Conclusion D-galac tose had different effects on bone of different gender rats. There is no effect on female bone, but it can induce the male rats osteoporosis. The mechanism of D-galactose on bone was probably due to the different effects on gonad glan ds.

Objective To observe the clinical efficacy of autologous peripheral blood stem cells (PBSC) transplantation in 26 cases of diabetic foot.Methods From 2003-11 to 2004-07,26 patients with diabetic foot received rhG-CSF 450~600ug/d by hypodermic injection for 5 days to mobilize stem cells.On the sixth day,PBSC were collected by COBE 6.1 Spectra Version for 82~148ml.The PBSC were injected into the ischemic lower limbs and foot intramuscularly for 3?3 cm distance.The clinical and laboratory findings were monitored.Results In 26 patients receiving PBSC transplantation,obvious pain-relief was shown in 23 cases for 3~14 days,foot cold-feeling changed in 24 patients after 2~7 days,foot ulcer improved in 4 cases after 4~12 weeks.ABI increased in 6 cases.Digital subtraction angiographic scores were peformed in 2 patients after 8 weeks,and there was new collateral vessels formation.No related complication or adverse effects were observed in all process.Conclusion Autologous PBSC transplantation might be a safe and effctive method for diabetic foot.

Objective To observe the clinical efficacy and interrelated factors of autologous peripheral blood stem cells (PBSC) transplantation in the treatment of arteriosclerosis obliterans (ASO) of the lower extremity. Methodes A total of 26 patients with ASO of the lower limb received rhG-CSF 450-600?g/d by hypodermic injection for 5 days to mobilize stem cells. On the sixth day, PBSC were collected by COBE 6.1 Spectra Version with a total amount of 82-118ml, with the number of mononuclear cells (MNC) (718.2-224.6)?10 9 /L. The MNC were injected intramuscularly into the ischemic limbs with intervals of 3cm apart. The clinical and laboratory findings were monitored. Results In 23 out of 26, pain was relieved after 3-21 days (88.5%), in 24 patients coldness was ameliorated after 2-28 days (92.3%), in 4 patients foot ulcers were better after 4-12 weeks. ABI increased in 6 cases. The total effective rate was 92.3%. No complication or adverse effect was observed in all the patients. Conclusion Autologous PBSC transplantation might be a safe and effective method for ASO of the lower limb ASO.

Objective To obtain the most effective preventive management for corticosteroid-induced osteoporosis by the evidence-based medicine approach.Methods We attempted to find the current best evidence for the prevention of corticosteroid-induced osteoporosis by searching ACP Journal Club(1991-Sep.2005),USA Agency for Healthcare and Research evidence report,Cochrane Library(Issue3,2005)and MEDLINE(1990-Sep.2005),and further critically appraised the available evidence.Results We found that VitD or its analogues with calcium and bisphosphonates could improve bone mineral density significantly.But the effects on reducing the development of fracture were not concluded.Conclusion VitD or its analogues with calcium can be the preferential method as prevention for corticosteroid-induced osteoporosis,while bisphosphonates may be an alternative way.