Objective To observe the situation of sexual life of gynecological cancer patients after surgery and explore the pertinent nursing intervention.Methods 400 patients with gynecological cancer and undergoing surgical treatment were selected in our hospital from February 2009 to September 2011.They were divided into the intervention group and the routine group with 200 cases in each group.The sexual psychological change,recovery of sexual life of patients of different types,sex frequency before and after treatment,and satisfaction degree with sexual life after positive nursing intervention were observed using phenomenological analysis.Results The satisfaction degree with sexual life reached 88％ in the intervention group after operation,significantly higher than 52％ in the routine group.Evident psychological changes were seen in patients with younger age,low-level education background,long treatment course and better economic condition.Conclusions Positive nursing intervention and guidance should be given to postoperative gynecological tumor patients in order to help them improve the quality of life and social status.

Objective To discuss the effects of nursing intervention under the guidance of health belief on enteroscopy compliance of colorectal cancer high-risk population. Methods A total of 260 people, who were clearly identified as colorectal cancer high-risk population during preliminary screening of 2 communities in Nankai District, Tianjin but had not finished enteroscopy, were selected and divided them into intervention group and control group with 130 people per group by random digits table method. In the intervention group, subjects received nursing intervention with guidance of the health belief model for a year, while in the control group, subjects simply received regular propaganda and education about colorectal cancer screening. Results After intervention, the cognitive levels in colorectal cancer screening, enteroscopy screening, high-risk factors was (9.16±2.95), (14.55±3.41), (14.25±4.26) points in intervention group and (7.28 ± 3.26), (12.23 ± 3.72), (13.12 ± 4.11) points in control group, and there was significant difference between two groups (t=4.855, 5.228, 2.180, P<0.01 or 0.05). After intervention, the scores of health belief including susceptibility, benefits, barriers, self-efficacy was (7.74 ± 1.50), (13.20 ± 2.45), (6.59 ± 1.91), (23.54±3.16) points in intervention group, and (6.68 ± 1.47), (11.58 ± 2.15), (8.42 ± 1.99), (20.82 ± 3.02) points in control group, and there was significant difference between two groups(t=5.730, 5.682,-7.561, 7.096, P<0.01). The rate of enteroscopy screening was 20.47%(26/127) in intervention group and 8.87%(11/124) in control group, and there was significant difference between two groups(χ2=6.719, P<0.05). Conclusions The nursing intervention under the guidance of health belief helps colorectal cancer high-risk population establish health belief, enhances participation rates of colonoscopy screening and has important significance to positive rates of early colorectal cancer.

Objective To explore the effects of lithium on expressions of cyclin-dependent kinase 5 (CDK5) and protein phosphatase 2A (PP2A) in the brains of chronic aluminum exposure rats so as to further understand the mechanism of lithium's inhibition on tau phosphorylation. Methods We divided 12 chronic aluminum chloride exposure rats into treatment group and non-treatment group (6 rats in each). Treatment group was given lithium chloride (200 mg/kg?d) via gastric perfusion daily for 6 weeks,while the non-treatment group sodium chloride at the same dosage and the normal group (6 non-aluminum exposure rats of the same month old) without intervention. Six weeks later,all the rats received Morris water maze test for learning memory function; CDK5 and PP2A expressions and phosphorylated tau protein level in rat hippocampus were determined by Western blotting. Results Compared with normal group,non-treatment of chronic aluminum exposure group showed higher phosphorylated tau protein level and CDK5 expression in the brain (P0.05). Conclusion Lithium may reduce tau phosphorylation and neurofibrillary tangle formation by inhibiting the expression of CDK5.

Objective To investigate the application of colposcope in cervical cancer screening in Yunnan ethnic minority areas.Methods The questionnaire survey was conducted on 641 women,and the data of the colposcopy and biopsy were reviewed retrospectively.The colposcopy and pathological diagnosis results were compared.Results The minority women thought colposcopy more acceptable was 78.64％ (383/487).The colposcopy found suspected low-grade cervical lesions were 377 cases,high-grade cervical lesions were 245 cases,cervical condyloma were 15 cases,cervical cancer were 4 cases.The pathological results:278 cases of cervical intraepithelial neoplasia (CIN) Ⅰ,174 cases of CIN Ⅱ,55 cases of CIN Ⅲ,15 cases of cervical condyloma,4 cases of cervical cancer.The consistent rate of colposcopy and pathological examination was 68.17％ (257/377) in low-grade lesions,89.39％ (219/245) in high-grade lesions,cervical cancer and cervical condyloma was entirely consistent.Conclusions The colposcopy is more easily accepted in Yunnan ethnic minority areas,which could be a preliminary diagnosis of cervical lesions,cervical condyloma and cervical cancer.It has an important clinical application value in diagnosis of cervical diseases.

Objective To screen the sensitive plasma molecular markers in diabetic nephropathy ( DN) patients with kidney yang deficiency. Methods Two-dimensional fluorescence difference gel electrophoresis (2-D DIGE) was used to analyze the plasma which was sampled from 4 early DN patients with kidney yang deficiency and 4 healthy adult volunteers. Proteins that showed differential expression with an over 1.5-fold change were analyzed by MALDI-TOF/TOF mass spectrometry. Results Two-dimensional electrophoresis (2-DE) maps of plasma proteins in DN patients with kidney yang deficiency and healthy adults were established successfully. We validated 9 differentially expressed proteins, including complements C3 and C4, apolipoprotein E and ubiquitination factor. Conclusion Proteomic analysis by 2-D DIGE can objectively reveal the differences of plasma protein expression in DN patents with kidney yang deficiency and healthy adult volunteers. The obtained 9 proteins have potential to provide plasma molecular markers for the early clinical diagnosis and for the research of traditional Chinese medical patterns of DN.

Objective To explore the sensitive blood plasma molecular markers in diabetic nephropathy (DN) .Methods Two-di-mensional fluorescence difference gel electrophoresis (2D-DIGE) was used to analyze early DN patients plasma (n=8) and normal plasma ,proteins that showed differential expression of a 1 .5 fold change were analyzed by MALDI-TOF/TOF mass spectrometry . Results 2D-DIGE maps of plasma proteins in patients with DN and normal plasma were established successfully .We validated 13 differentially expressed proteins detected by 2D-DIGE ,including complement component C3、complement component C4、apolipopro-tein E ,etc .Conclusion Proteomic analysis can objectively revealed the differences of protein expression between the two kinds of blood plasma .The 13 proteins might be the potential plasma molecular markers for the clinical diagnosis of diabetic nephropathy .

Objective　To study the possibility of measuring health status for residents in rural areas by the Chinese SF-36 scale. Methods　Health status for 207 residents in rural areas of Fuyang city who were 14 years old and over was measured by the Chinese SF-36 scale. The reliability and validity were analyzed. Results　The internal consistency reliability coefficients (Cronbach'α) exceeded 0.50 for all scales except SF and RE scales. Inter-scale correlation coefficients were lower than Cronbach'α coefficients for all scales except SF scale. Factor analysis identified three principal components, which could be used to explain 64.6% of the total variance. The differences of scale scores between the young group and the middle age group weren't significant. There weren't significant differences of scale scores between sexes except RP scale. The differences of scale scores between smokers and no-smokers, drinkers and no-drinkers weren't significant. Nor were the differences between various educational levels. Conclusion　Whether the Chinese SF-36 scale is suitable for measurement of health status for residents in rural areas needs further studies to give more evidences.

Traditional Chinese medicine (TCM) is a great knowledge base that has been developed through thousands of years and has been accumulated a wealth of experience in diagnosis and treatment.TCM has advanced concepts,but lag behind the use of modern technology and means.With the progress of modern science and technology,the combination of TCM theory and moderm science and technology promotes the development of TCM engineering.TCM has been a breakthrough in diagnostic techniques and clinical treatment,especially modern TCM instruments.At present,diagnostic instruments of TCM mainly depend on pulse and tongue device,and gradually develop to four diagnostic comprehensive analysis platform.Therapeutic instruments of TCM include electroacupuncture device,meridian instrument,laser treatment device and magnetic therapy instrument.This article focuses on the current status,problems and trend on TCM diagnostic and therapeutic instruments,which mainly include diagnostic instruments,clinical treatment instruments,Chinese medical instruments.

The effect of tumor necrosis factor (TNF) in combination with thymosin and chemotherapy on advanced primary liver cancer was observed. Eighty-nine advanced primary liver cancer cases were randomized into two groups: Patients in the control group were treated only with chemotheraputic drugs, and patients in the observing group were treated with TNF, thymosin and chemotheraputic drugs. The results showed that there were higher primary tumor regression rates and statistically reduced AFP in the observing group than control group. The rate of half year survival, one year survival and two years survival improved 28.6%, 18.0% and 5.1% than those of control group, respectively. We belived that this combined therapy could effectivelly improve the patients immunity, the quality of survival, and long the patients survival period.

Objective To observe the effect of therapeutics of invigorating spleen and kidney, removing blood stasis and clearing away turbidness on lipid metabolism and renal function in patients with chronic renal failure (CRF). Methods Fifty-eight patients with CRF were randomly divided into the treated group (30 cases) and the control group (28 cases). Both groups were treated with low-protein diets, controlling hypertension and symptomatic treatment. And Chinese drugs for invigorating spleen and kidney, removing blood stasis and clearing away turbidness were given additionally to the treated group. The curative effect, renal function and lipid metabolism were observed after two months. Results TC, TG and APOB in the treated group lowered significantly, HDL-C and APOAⅠraised significantly after treatment (P0.05). The total effective rate and the improvement of renal function in the treated group were better than those in the control group (P