Gene vector is defined as a tool which can carry and transfer gene or other nucleic acid material into cells. Its chemical nature can be protein or polypeptide, nucleic acid, lipide, carbohydrate, and other polar molecule or its compound. As an.important part of gene therapy, gene delivery system is also the bottleneck of gene therapy. There are two existent gene vectors defined as viral vectosr and non-viral vectors. Generally, high transfection efficiency can be achieved by the application of viral vectors. But the application of viral vectors has been restricted by its immunogenicity and mutngenic potential as side effects. As a novel gene delivery system, non-viral vectors have the advantages of low toxicity, low immunogenicity, and relative targeting effect. This article provides a review on the latest research progress about various kinds of vectors.

Objective To evaluate the application efficacy of responsibility system for holistic nursing system in high-quality nursing service in the department of respiratory medicine.Methods Department of respiratory medicine unit which was the quality nursing service demonstrated unit in our hospital was selected.The nursing responsibility-bound grouping system was first performed and the nurse-incharge would provide the patients with whole and continuous nursing service.Before and 6 months after the application,the nursing job satisfaction was conducted and the nursing grouping system satisfaction was also evaluated among nurses and doctors.Results Six months after the application of nursing responsibility-bound grouping system,the patient satisfaction with nursing job was raised obviously in the unit.Conclusions The application of responsibility-bound grouping system can not only promote the activity but also mobilize the enthusiasm of nurses,and then high-quality nursing service was resulted.So the service system was worthy of clinical application.

Objective To explore the effect of dexmedetomidine on the stress and immunologic function in the patients undergoing uterectomy,and to provide an evidence for the medication in clinical anesthesia.Methods A total of 60 hysteromyoma patients who needed uterectomy were selected.All the patients were in ASAⅠ-Ⅱlevel. The patients were divided into observation group (n=30)and control group (n=30)according to administration of different types of drugs. Before anesthesia, the patients in observation group were given dexmedetomidine (l μg·kg-1 )with a concentration of 4 g· L-1 for 10 min,while the patients in control group were given equal normal saline.The induction was performed 10 min after infusion pump.Sevoflurane and fentanyl were used for maintaining during the anesthesia.The blood samples of the patients in two groups before entering the operation room (T0),2 h after operation (T1),and extubation after operation (T2)were collected.The serum cortisol (COR)and interleukin-2 (IL-2)concentrations,plasma T lymphocyte subpopulations and NK levels were detected. Results The differences of COR and IL-2 concentrations at T0 between two groups were not statistically significant (P >0.05).The COR concentrations at T2 in two groups were significantly higher than those at T0 (P <0.05). The COR concentration at T2 in observation group was significantly lower than that in control group (P <0.05). The serum IL-2 concentration was gradually decreased with the prolongation of time observation in two groups.The serum IL-2 concentration in observation group was significantly higher than that in control group (P <0.05).The differences of CD3+ ,CD4+ ,CD8+ ,CD4+/ CD8+ ,and NK levels at T0 between two groups were not statistically significant (P >0.05).The differences of CD8+ level in each time point between two groups were not statistically significant (P > 0.05).The CD3+ ,CD4+ ,CD4+/ CD8+ , and NK levels at T1 and T2 in control group were significantly lower than those at T0 (P < 0.05 ). The CD3+ , CD4+ , CD4+/ CD8+ , and NK levels at T2 in observation group were significantly higher than those in control group (P <0.05).Conclusion Dexmedetomidine assisting anesthesia can alleviate the postoperative stress reaction,and reduce the effect of anesthesia and operation wound on the cellular immune function.It is beneficial for the postoperative rehabilitation.

Objective:To investigate the effects of fluvastatin on high-sensitivity c-reactive protein( hs-CRP) , tumor necrosis fac-tor-α(TNF-α), urine albumer excretion rate (UAER)and serum creatinine (Cr) in the patients with early diabetic nephropathy. Methods:Totally 69 patients with early diabetic nephropathy were randomly divided into the observation group with 34 cases and the control group with 35 cases. The observation group was treated by low-protein diet plus fluvastatin, and the control group was treated by low-protein diet only. The course of treatment was 8 weeks. Totally 65 nondiabetic persons were selected as the normal group, and the levels of TNF-α, hs-CRP, UAER, ALT and Cr were detected before and after the treatment. Results:The levels of TNF-α, hs-CRP and UAER in the observation group and the control group were significantly higher than those in the normal group(P<0. 05);the lev-els of ALT and Cr showed no significant differences (P>0. 05). Compared with that before the treatment, the level of UAER was de-creased significantly in the control group after the treatment (P<0. 05), and the levels of TNF-α and hs-CRP showed no significant differences after the treatment (P>0. 05). In the observation group, the levels of TNF-α, hs-CRP and UAER were all decreased sig-nificantly after the treatment (P<0. 05). Conclusion:UAER, hs-CRP and TNF-α are closely connected with diabetic nephropathy. Fluvastatin can decrease the levels of TNF-α, hs-CRP and UAER. Low-protein diet plus fluvastatin is effective and safe in the treat-ment of early diabetic nephropathy, and the efficacy is superior to that of low-protein diet only.

To raise the quality of pathology teaching and students' abilities of possessing knowl-edge,we reformed the teaching method. Applying information feedback between teachers and students in pathological teaching can effectively solve students' confusion and improve teacher's teaching quality. Stu-dents' interests and initiative in pathology learning were aroused and teachers' responsibilities were pro-moted by teaching reform,thus having satisfactory results were obtained.

ObjectiveTo observe the clinical efficacy of acupuncture plus TDP (specific electromagnetic treatment apparatus) and auricular point sticking in treating chronic pelvic infection.MethodSixty patients diagnosed with chronic pelvic infection were randomized into two groups. Thirty cases in the treatment group were intervened by acupuncture plus TDP and auricular point sticking, while the other 30 in the control group were by acupuncture plus TDP, 10 d as a treatment course in both groups, and the therapeutic efficacies were compared after 3 courses.ResultThe total effective rate was 96.7% in the treatment group versus 83.3% in the control group, and the difference was statistically significant (P<0.05).ConclusionAcupuncture plus TDP and auricular point sticking can produce a marked therapeutic efficacy in treating chronic pelvic infection, and its total effective rate is higher than that of acupuncture plus TDP.

AIM: To optimize the extraction process for the active components in Curcuma by supercritical CO_2 as guided by the contents of curcumol in Curcuma. METHODS: Gas chromatography was used to determine the contents of curcumol in the extract.Uniform design and path analysis were used to optimize SFE process. RESULTS: The optimum process was established as following:20 MPa as extracting pressure,40℃ as extracting temperature,120 minutes as dynamic extracting time and 20 minutes as static extracting time,24 mL of 70% alcohol as modifier.The absolute error of the predicted value from the model was smaller than 0.6%. CONCLUSION: The regression model is very notable and reasonable and can forecast results precisely.

Objective To explore the characteristics of TCM syndrome of vertigo. Method Patients of vertigo need to fill a form about the symptoms of TCM, and then analyze the frequence and percentage of the symptoms and the syndromes with SPSS software. Result Except vertigo or dizziness, the common accompanied symptoms were distractedness, feeling thirsty, tinnitus, poor memory, afraid of cold and vomiting, etc. There were 26 syndromes, and the top 5 of which are yin-deficiency of liver and kidney, wind stirring, phlegm blocking, qi-deficiency of spleen and blocking qi of liver. Conclusion The characteristics of syndrome of these patients of vertigo were same as the theory of TCM. The better way to find the characteristics of syndrome of vertigo still need to be explored.