OBJECTIVE:To investigate the effect of sertraline on cytokine balance in patients with depression. METHODS: Serum levels of interleukin2(IL-2), interleukin12(IL-12), interleukin4(IL-4), tumor necrosis factor-alpha (TNF-?) and transforming growth factor beta (TGF-?1) were measured in 26 patients with depression(depression group) before and after treatment and 28 healthy volunteers (control group) by enzyme linked immunosorbent assay (ELISA). RESULTS: As compared with control group, in depression group the serum levels of IL-2, IL-12, TNF-? were significantly higher than in control group; the serum levels of IL-4, TGF-?1 were significantly lower than in control group(P

Objective To research the effects of different titers of bacteriophage D29 on growth and function of airway epithelial cell 9HTE.Methods Cell viability rates was analyzed after applying high(109 PFU/mL)and low(107 PFU/mL)titers of bacteriophage D29 and phage buffer respectively by MTT colorimetry.Additionally,the secretion levels of IL-6,IL-8 in cell culture supernatant were detected by ELISA.RT-PCR was performed to detect the expression of ICAM-1 mRNA.Cell apoptosis rate was analyzed by flow cytometry.Results There was no difference in cell growth,secretion levels of IL-6,IL-8,ICAM-1 mRNA and cell apoptosis rate between cells treated with high and low titers of D29 and phage buffer(P>0.05).Conclusion Neither high nor low titer of bacteriophage D29 exerts effect on growth and function of airway epithelial cell 9HTE in vitro.

Objective To study the signaling transduction of dBcAMP induced morphological changes of astrocytes.Methods Morphological changes of primarily cultured mouse astrocytes induced by dBcAMP were studied by immunocytochemical technique.The signaling transduction pathways underlying those changes were further explored using specific inhibitors AG1478 and GM6001.Results Zn2＋-dependent metalloproteinase and epidermal growth factor receptor（EGFR）in astrocytes were involved in the morphological changes of astrocytes induced by dBcAMP.Conclusion dBcAMP-induced transactivation pathways were delineated in the morphological changes of astrocytes.

ObjectiveTo observe the spinal cord pathological change and hindlimbs motor function of rats after decompression on chronic spinal cord injury.Methods 30 Wistar rats were divided randomly into conrol group ( n =5 ),compression group ( n =5 ) and decompression group ( n =20 ),then the decompression group was subdivided into 4 parts as 1 week,2 weeks,4 weeks,6 weeks after decompression.Decompression was made after 30 days of chronic spinal cord copression injury,rats'motor function was detected by inclined plate experiment,the histopathological change,Nissl body and neural cells apoptosis in different spinal cord sections were assessed by the stainings of HE,Nissl and TUNEL.ResultsCompared with control group,the behavior testing showed all rats in compression group presented with weakness of muscle power in their hindlimbs (P ＜ 0.01 ),but the hindlimbs motor functions recovered from the first week after decompression and the difference had statistical significance compared with the compression group(P＜ 0.01 ).Then the rats hindlimbs functions recovered gradually later on.The staining of HE,Nissl and TUNEL showed that the injured spinal cord section performed no improvement at the first 2 weeks after decompression,the neural apoptosis index(24.31 ± 4.73 )％ decreased until the forth week after decompression(P＜0.05).The spinal cord segments closed to the injured part recovered in early stage.At 1 week after decompression,lots of Nissl bodys were observed in spinal anterior horn,the neural apoptosis index in the 2 sections closed to the injured part were ( 15.21 ± 4.81 ) ％ and ( 16.21 ± 3.98 ) ％,which declined observably compared with compression group(P ＜ 0.05 ).ConclusionAfter decompression on chronic spinal cord injury,the recovery of rats'hindlimbs motor function in early stage is probably benefited by the functional compensation of the spinal cord segments closed to the injured section.

Objective To study the part of Anti-thrombus and its property from Ground Beetle. Methods To dispart the Ground beetle into four segments which were shell, head, chest and abdomen, and adopt the method of frozen and melt to do the further test. Result Extracts of shell and chest were not active, extracts of head and abdomen were active. The activity of abdomen extracts was much better than that of the insect extracts. Its active component was glycoproteins. Conclusion The activity of abdomen extracts (Tf) is relatively high, and demonstrates wider value.

Objective To establish a method to determine six kinds of PAEs in human serum simultaneously,and on this basis to understand the level of PAEs in human body.Methods Ultrasonic extraction,gas chromatography-mass spectrometry-selected ion monitoring(GC-MS-SIM) detection and quantitative analysis based on internal standard were used to detect six kinds of PAEs in human serum simultaneously.Finally this method was used in fifty human serum samples.Results The results showed that this method had a good linear relation in the range from 10 to 1 000 ng/ml,and the correlation coefficients of standard curve were higher than 0.999.The average recovery rate and the relative standard deviation(RSD,n=6) of the target compounds in standard-addition blank was 94.5% and 4.4% respectively,the lower limits of detection for DEP,DMP,DBP,BBP,DEHP,DNOP were 0.31,0.85,0.90,0.63,0.88,0.41 ng/ml respectively,and the recovery rates of all samples ranged from 67.98% to 110.8%.Conclusion This method has good recovery rate,reproducibility,lower limit of detection and can be used for the determination of many kinds of PAEs substances in human serum.

Objective To explore the relationship between angiogenesis and ischemic neurological deficit repair through exogenous interventions,and investigate optimal intervention routes for angiogenesis in cerebral ischemia rats.Methods Totally 240 SD rats were randomly divided into sham operation group,ischemic control group,ischemia and vascular endothelial growth factor(VEGF) group [1 653.3 ng/(g?d),i.p.,for 10 d],ischemia and endothelial progenitor cell transplantation(EPCs) group(2?1010/L,10 ?l),ischemia and anti-VEGF monoclonal antibody group [3 mg/(kg?d),i.p.,for 10 d].Cerebral microvessel density,somatosensory evoked potentials(SEP) and motor evoked potentials(MEP) and behavior were observed in 1,3,7,14,30 and 60 d after injury.Results New blood vessel density of ischemia and VEGF group,ischemia and EPCs group were significantly higher than ischemic control group in all time points besides 1 d after injury(P

Objective Testing the CRP and PLT for patients with acute myocardium infarction(AMI) .Analysising the relation‐ship between AMI and CPR or PLT .Methods We chose 60 patients with AMI who treat in our hospital during 2012/11 and 2014/10 as an observation group .As the same time ,we also chose 60 healthy person as a comparison group .Testing the CRP and PLT of the two groups with the same method ,and then we compare and analysis the results .Results The observation group's concentration of CRP is (22 .13 ± 4 .71)mg/L ,level of PLT is (241 ± 33)× 109/L .The comparison group's concentration of CRP is(2 .74 ± 0 .49) mg/L ,level of PLT is(162 ± 26) × 109/L .The result of the observation group is obvious higher than the comparison group ,and the difference is significance(P<0 .05) .The observation group's positive rate of CRP is 73 .33% ,increase of PLT is 38 .33% .The comparison group's positive rate of CRP is 3 .33% ,increase of PLT is 5 .00% .The result of the observation group is obvious higher than the comparison group ,and the difference is significance(P<0 .05) .Conclusion To the clinical diagnosis of AMI ,testing the CRP concentration and the PLT level is useful to understand the patient's host defenses and inflammation condition .It has clinical value to AM I's prevent ,diagnosis and prognosis .

A retrospective study was conducted based on the clinical data of 15 choledocholith patients after Billroth-Ⅱgastroenterestomy who were treated with endoscopic retrograde cholangio-pancreatography (ERCP) through colonoscopy or duodenoscopy.It showed that choledocholith removed by colonoscopy for patients with Billroth-Ⅱ gastroenterestomy can achieve comparable therapeutic effect,but this method can reduce the operation difficulty,shorten the operation time and significantly increase the success rate.

Objective To study the diagnostic value of the golgi protein 73 (GP73) combined with alpha‐fetoprotein (AFP) ,α‐L‐fucosidase (AFU) andα‐antitrypsin(α‐AT) detection in primary hepatic carcinoma(PHC) .Methods 100 patients with PHC ,100 patients with hepatitis and cirrhosis ,and 100 healthy people hospitalized in the hospital from October 2011 to May 2013 were en‐rolled in the study .Serum AFP levels were detected by using Beckman DxI800 chemiluminescent analyzer ,serum AFU ,α‐AT levels were detected by using Beckman DxC800 automatic biochemical analyzer ,and GP73 levels were measured by using enzyme‐linked immunosorbent GP73 quantitative detection kits .Results In patients with PHC and patients with hepatitis and cirrhosis ,serum GP73 ,AFP ,AFU ,α‐AT levels were significantly higher than healthy people(P<0 .05) .In patients with PHC ,GP73 ,AFP ,AFU ,α‐AT levels were significantly higher than those in patients with hepatitis and cirrhosis(P<0 .05) .The sensitivities of single serum GP73 ,AFP ,AFU ,α‐AT detection were 75% ,72% ,23% ,67% respectively and specificity were 95% ,95% ,100% ,97% respective‐ly .The sensitivity and specificity of the 4 tumor markers′combined detection were 99% and 100% .The sensitivity of combined de‐tection was significantly different from single detection(P<0 .05) .Conclusion GP73 ,AFP ,AFU ,α‐AT may improve the diagnostic efficiency of primary liver cancer .