OBJECTIVE: To establish the HPLC method for the determination of chloramphenicol and dapsone in compound chloramphenicol cream. METHODS: The analytical column was Hypersil ODS and the mobile phase consisted of 0.1%sodium heptanesulfonate solution (a mixture of 0.1% sodium heptanesulfonate 500 mL and dimethylformamide 5 mL plus acetic acid glacial 0.5 mL)-acetonitrile (75∶25) at a flow rate of 1.0 mL?min-1. The detection wavelength was 272 nm and the sample size was 20 ?L. RESULTS: The linear ranges of chloramphenicol and dapsone were 25.59～153.54 ?g?mL-1 (r=0.999 9) and 12.57～75.42 ?g?mL-1 (r=0.999 9), respectively. The average recovery rates were 100.62% (RSD=0.92%) and 100.93% (RSD=1.22%), respectively. CONCLUSION: The method is simple and accurate and can be used for the quality control of compound chloramphenicol cream.

Objective To establish clonal human embryonic stem cell lines and investigate their biological characteristics. Methods Cells were derived from one inner cell mass of human blastocyst, multiplied for 20 passages, and then dissociated into single cell suspension by digestion with 0.5% trypsin. Single cell was picked up and plated into individual well of a 96-well plate containing feeder-layers directly under a dissection microscope. The outgrowth clones were passed by treatment with collagenase. Surface markers were detected by cytochemistry and histoimmunochemistry. Karyotypes were tested using standard G-banding techniques. The pluripotency was analyzed by inoculating cells into severe combined immunodeficient (SCID) mice. Results Two clonal human embryonic stem cell lines were established. Cells of these two lines possess the characteristics and differentiating potencies: normal 46 XX karyotypes; expressing a series of surface markers such as: alkaline phosphotase, stage-specific embryonic antigen (SSEA)-4, tumor recognition antigen (TRA)-1-60, TRA-1-81 etc; and forming teratomas comprising derivatives of three embryonic germ layers such as neural tissue, cartilage, squamous epithelium and columlar epithelium when injected into SCID mice. Conclusions The two single cell-cloned human embryonic stem (hES) cell lines were derived in our laboratory. The cells possess stable biological characteristics of undifferentiated hES cells.

Objective:To study the effect of cervical canal mucosa dng excision on cervical columnar eversion in Loop electrosurgical excision procedure(LEEP).Methods:A prospective randomized control trail was performed in 125 cervical intraepithelial neoplasia(CIN)patients.Cervix and cervical canal were conically excised with triangle electrode in 62 patients in the control group;in the study group,a small ring electrode was put into the cervical canal and the cervical canal mucosa was excised about 0.5～0.8cm,after excision as the control group.Follow-up was performed postoperatively in 1,3,6 months respectively.The operation time,the bleeding volume,the rate of cervical columnar eversion and cervical adhesion or stenosis were compared between the two groups.Results:The rate of cervical columnar eversion in the study group (1/63,1.59%)was lower than that in the control group(9/62,14.52%).There was significantly statistical difference between them(P=0.008).There was no statistical differences between them in the operation time,the bleeding volume,and the rate of cervical adhesion or stenosis (P>0.05).Conclusions:The cervical canal mucosa ring excision in LEEP can effectively prevent postoperative cervical columnar eversion.

The research of curriculum tells us that in every university there are two kinds of curriculums.One is noticeable,the other is hidden.Both of them work for the goal of bringing the students up.The witchery of hidden curriculum is that it affects the students continually and secretly.This essay discuses the significance,principle and approaches of the construction of hidden curriculum in higher learning.

Objective To assess the status of mental health of nursing students of secondary technical school and provide targeted guidance for mental health education. Methods 642 nursing students of secondary technical school were assessed using Mental Health Diagnostic Test Scale (MHT Scale). Results There were 0.78% of nursing students with serious psychological problems (scores≥65), 4.52% of nursing students with less serious psychological problems (scores < 65 and≥ 56). Students in different grades had significant difference in learning anxiety, allergic tendency, physical symptoms anxiety. Conclusions Nursing students of secondary technical school were in good mental health in general, but there were many students had learning anxiety, allergic tendencies and physical symptoms anxiety, which were different among various grades. Secondary nursing schools should further strengthen the mental health education, and seek close collaboration with parents,provide appropriate intervention measures to the nursing students with psychological barriers, in order to enhance the overall mental health of nursing students.

OBJECTIVE To investigate the distribution and tendency towards drug resistance of Candida albicans which causing clinical deep infection and to supply data to clinical treatment. METHODS The distribution and tendency towards drug resistance of C. albicans isolates from infected patients from Jan 2007 to Dec 2007 were analyzed retrospectively.RESULTS All 1698 strains of Candida were isolated from patients sputum,urine,blood,secretion,etc. From 1075 cases with C. albicans,965(89.8%) strains were isolated from sputum. The resistance rate to nyststin,fluconazole,itraconazole,5-fluorocytosine and amphotericin B were 3.9%,3.6%,2.5%,0.5% and 0,respectively. The factors related to nosocomial C. albicans infection were the use of antibiotics and condition,invasive procedure,physical fitness,age,basic state of patients,etc.CONCLUSIONS The incidence and resistance of C. albicans infection in a hospital have increasing by years. Proper use of antibiotics and immunodepressors,reduction of unnecessary operation,and early diagnosis and treatment are the keys in preventing from systemic C. albicans infection.

Objective To observe the clinical efficacy of Lao Shi Jiu (moxibustion at a group of ten acupoints) plus Wu Zhu Xiang Sha Liu Jun decoction in treating chronic gastritis due to deficient cold in spleen-stomach. Method Ninety patients with chronic gastritis due to deficient cold in spleen-stomach were randomized into a moxibustion-medication group and a Western medication group by using the random number table, 45 cases each. The moxibustion-medication group was intervened by Lao Shi Jiu plus Wu Zhu Xiang Sha Liu Jun decoction, while the Western medication group was prescribed with conventional medications. The clinical efficacy and changes in symptoms were observed after the intervention. Result The total effective rates were respectively 88.9% and 75.6% in the moxibustion-medication group and Western medication group, and the rate in the moxibustion-medication group was significantly higher than that in the Western medication group (P<0.05); the gastroscopy-based total effective rates were respectively 91.1% and 77.8% in the moxibustion-medication group and Western medication group, indicating that the moxibustion-medication group was superior to the Western medication group (P<0.05); the improvement of stomachache in the moxibustion-medication group was more significant than that in the Western medication group (P<0.05). Conclusion The moxibustion-medication group is superior to the Western medication group in comparing the clinical efficacy and improvement of symptoms in treating chronic gastritis due to deficient cold in spleen-stomach.

Objective: To study long time aerobic training effect on mitochondrial DNA (mtDNA) content and activities of mitochondrial respiratory chain complexes in the myocardium and brain tissues of aged rats. Method: Forty-five rats were randomly divided into three groups Group A(n=15)younger rats did not received training; Group B (n=15) old rats did not received training; Group C (n=15) old rats received gradually training for 90 days. The mtDNA content were determined by methods of Yah erc, the activity of mitochondrial respiratory chain complexes in myocardium and brain tissues were determined by methods of Wu etc. Result: The mitochondrial DNA content in myocardium and brain tissues significantly increased (P<0.01) and the activity of mitochondrial respiratory chain complexes in myocardium and brain tissues significantly decreased (P<0.05-0.01) in the aging control group as compared with those in the young group. The mitochondrial DNA content in myocardium significantly decreased (P< 0.05), while there was no significant difference in brain. The activity of mitochondrial respiratory chain complexes in myocardium and brain tissues significantly incroased(P<0.05-0.01) in the aging training group as compared with those in the aging control group. Conclusion: the long time aerobic training could decline the mitochondrial DNA content and increase the activity of mitochondrial respiratory chain complexes in myocardium and brain tissues in aging rats.

Strengthening the construction of open optional courses of medicine in universities is necessary for us to make good use of the teaching resources,to optimize the open optional courses system,and to improve the quality education.This thesis analyzes the problems of open optional courses of medicine in the university by questionnaire.It brings out ways to further improve the construction of open optional courses of medicine in universities.

Objective To test the reliability and validity of the Chinese version of Quality-of-Life Questionnaire for Mechanically Ventilated Patients (QOL-MV). Methods The English version of QOL-MV was revised and translated into Chinese version. The reliability and validity of the Chinese version of QOL-MV was tested in 120 mechanically ventilated patients. Results The Cronbachαcoefficient of the Chinese QOL-MV was 0.904. The item-level content validity index of Chinese QOL-MV were 0.86-1.00 and the scale-level content validity index of Chinese QOL-MV was 0.92. Two factors were extracted by factor analysis and the cumulated variance was 80.18%. The dimensional factor loading of each item was greater than 0.565. The correlation coefficient between the score of Chinese QOL-MV and the Euroqol Group′s 5-domain 3 Level Questionnaire (EQ-5D) and Richards Campbell Sleep Questionnaire (RCSQ) was 0.947 and 0.561 respectively (P<0.01). Conclusions The Chinese version of QOL-MV has been proved to be reliable and valid.It can be used to measure the quality-of-life for mechanically ventilated patients.