Hereditary Autoinflammatory Diseases ; Humans

Child ; Humans ; Tuberculosis ; mortality

Objective:To evaluate bacteriostasis effect of domestic-made water-soluble propolis on prevotella intermedia(Pi),fu-sobacterium nucleatum(Fn)and mixture bacteria of Pi and Fn in vitro.Methods:With agar dilution,minimal bactericidal con-centrations(MBC)of propolis for Fn,Pi and mixture bacteria were determined.Results:MBC of propolis for Fn,Pi or mixture bacteria were 0.025%,0.025%,0.05% respectively.Conclusion:Propolis shows the favourable antibacterial activity against the main anaerobic bacterium in infectious root canal which it has a value of application for disinfecting root canal.

Objective To observe the situation of sexual life of gynecological cancer patients after surgery and explore the pertinent nursing intervention.Methods 400 patients with gynecological cancer and undergoing surgical treatment were selected in our hospital from February 2009 to September 2011.They were divided into the intervention group and the routine group with 200 cases in each group.The sexual psychological change,recovery of sexual life of patients of different types,sex frequency before and after treatment,and satisfaction degree with sexual life after positive nursing intervention were observed using phenomenological analysis.Results The satisfaction degree with sexual life reached 88％ in the intervention group after operation,significantly higher than 52％ in the routine group.Evident psychological changes were seen in patients with younger age,low-level education background,long treatment course and better economic condition.Conclusions Positive nursing intervention and guidance should be given to postoperative gynecological tumor patients in order to help them improve the quality of life and social status.

OBJECTIVE:To study the flavonoids in Inula britannica.METHODS:The Inula britannica was extracted with 95% ethanol and isolated and purified by different column chromatography,the structures of the constituents were derived by measuring the physicochemical constants and spectral analysis.RESULTS:A total of 10 flavonoids were isolated from Inula britannica which were stated as follows:Spinacetin(1),6-methoxy-luteolin(2),Iuteolin(3),Quercetin(4),Isorhamnetin(5),Kaempferol(6),Rutin(7),Patulitrin(8),Isoquercitrin(9)and Quercitrin(10).Of which,compounds 5,7 and 10 were isolated from Inula britannica for the first time.CONCLUSION: This result serves as a scientific basis for the further study of Inula britannica.

Objective To compare the application of National Standard GB/T 20470-2006 and Industry Standard WS/T 403-2012 in quality management of biochemical detection .Methods Comparison of indexes such as σ level ,quality goal index (QGI ) , priority improvement measure and performance evaluation were conducted in projects of external quality assessment launched in this department .Results Differences of performance evaluation showed no statistically significant between National Standard GB /T 20470-2006 and Industry Standard WS/T 403-2012(P= 0 .069) ,while their differences of σ value classification and priority im-provement measure were statistically significant (P were 0 .007 ,0 .012 ,respectively ) .Conclusion Industry Standard WS/T 403-2012 is more stringent than National Standard GB/T 20470-2006 .

Objective To study on the effect of situational teaching based on ECS in medical nursing teaching.Methods 93 nursing students were divided into the experimental group (45 cases) and the control group (48 cases).The experimental group adopted ECS in the medical nursing situational teaching,while the control group used conventional teaching method.The teaching results between the two groups were compared between two groups.Results Two groups had significant differences in the medical nursing theory test and the operation skills examination.The students in the experimental group had higher recognition on the situational teaching based on ECS,especially in the learning initiative and interest,pathology observation ability and the unity cooperation ability,the differences showed statistical difference.Conclusions The application of ECS in the medical nursing situational teaching can stimulate the learning interest of the nursing students,help the cultivation of clinical thinking ability,relieve the contradiction of resources strain in nurses clinical probation and promoting the improvement of medical nursing teaching.

Objective To observe the effects of ganoderma lucidum polysaccharide(GLP) on intestine mucosal barrier function and tumor necrosis factor-α(TNF-α)in hemorrhagic shock rabbits. Methods Thirty healthy Japanese white rabbits were divided into three groups by random number table:sham operation group, resuscitation/reperfusion with normal saline(NS)group(NS group)and resuscitation/reperfusion with GLP group (GLP group). The hemorrhagic shock models were reproduced by being bled from the femoral artery to the mean arterial pressure(MAP)of 30-40 mmHg(1 mmHg=0.133 kPa)within 10 minutes and kept maintaining at this MAP level for 40 minutes. Then,the anti-shock therapy was given. The rabbits in NS group were immediately and quickly resuscitated with reperfusion of their shed blood and intravenous drip of NS in a volume equal to 2-fold of the volume of the shed blood. The rabbits in GLP group were dealt with the same means as NS group except NS was replaced by 1%GLP. The rabbits in sham operation group were only cannulated and maintained in a status of blood pressure measurement,but without bloodletting and resuscitation. The TNF-α content,the number of positive bacterial culture of blood bacteria were observed at the time before shock(S0),40 minutes after shock(S40)and 40 minutes and 90 minutes after resuscitation/reperfusion(R40,R90),respectively in the three groups. The serum endotoxin level and the TNF-αcontent in intestinal mucosa were examined at R90. Under the light microscopy,the pathological changes of intestinal mucosa were examined,and the score of the mucosal damage was evaluated by the criteria of Chiu method. Results ①Before shock,the results of blood bacterial culture of all animals in three groups were negative. With the extension of time,the positive rates of blood bacteria were increased gradually at R40 and R90 in NS and GLP groups,showing increment of bacterial translocation,but the levels at R40 and R90 were significantly lower in GLP group than those in NS group at the same time points(number of rabbits at the time of R40:2 vs. 4,at the time of R90:4 vs. 6,both P<0.05). Escherichia coli was the most common positive bacteria in the blood;the other positive bacteria were sequentially less and less,Enterococcus,Lactobacillus acidophilus and Staphylococcus aureus. Compared with sham operation group,the serum endotoxin levels of NS and GLP groups were significantly higher at R90(kU/L:1.823±0.963,1.361±0.529 vs. 0.064±0.036,both P<0.05). ② Under the light microscope,it was shown that in NS group the mucosal villi were edematous,the sub-epithelial space was widened,and there was infiltration of neutrophils and lymphocytes. The damage of intestinal mucosa in GLP group was obviously milder than that in the NS group. The Chiu scores of GLP and NS groups were significantly higher than that of sham operation group;meanwhile,Chiu score of GLP group was lower than that of NS group(1.44±0.64 vs. 3.79±1.23,P<0.05).③Along with extension of ischemia and reperfusion time,TNF-α contents in NS and GLP groups were gradually increased significantly,the peak value being at R90,meanwhile,the levels in both groups were significantly higher than the level of sham operation group,and the TNF-α content in plasma of GLP group was lower than that of NS group〔A value:33.350±7.950 vs. 85.080±4.330,P<0.05〕. Compared with sham operation group,the TNF-αcontents in intestinal mucosa of NS and GLP groups were higher than the level in sham operation group,meanwhile,TNF-αcontent in intestinal mucosa of GLP group was lower than that of NS group〔A value:96.38±8.59 vs. 167.73±12.32, P<0.05〕. Conclusion In the salvage process of resuscitation-reperfusion,GLP can protect intestinal barrier function in rabbits with hemorrhagic shock,and its effect may be related to antagonizing the release of TNF-α.

Objective To analyze the relationship between the status of anxiety and depression and activity and participation for inpa-tients with spinal cord injury (SCI). Methods From January, 2014 to June, 2016, a total of 37 inpatients with SCI were assessed by Zung Self-rating Anxiety Scale (SAS), Zung Self-rating Depression Scale (SDS), and WHO Disability Assessment Schedule 2.0 (WHO-DAS 2.0) at discharge. Results The average scores of SDA were (0.5±0.18) and SAS were (41.15±14.7) respectively. The inpatients scored (1.43± 0.82) (cognition), (3.06±1.43) (mobility), (2.47±1.11) (self-care), (2.13±0.66) (getting around), (3.68±1.32) (life activity for household and work), and (3.57±0.84) (participation) in six domains of WHO-DAS 2.0 respectively. There were severe disabilities in mobility, life activity for household and work, and participation;mild disability in cognition;and moderate disabilities in self-care and getting around. There was positive correlation between depression and functions of mobility, life activity for household and work, and participation (P<0.05). Conclu-sion Depression influences the status of activity and participation for inpatients with SCI at recovery stage.

Objective To study the clinical value of ultrasonography in the diagnosis of ketamine associated cystitis. Methods 60 cases were confirmed by pathological examination to determine the occurrence of ketamine associated cystitis in clinic, all patients underwent ultrasound examination, recording bladder morphology, wall thickness, cavity size (thickness, diameter, length and diameter), calculation of residual urine volume and bladder capacity according to the relevant data. The pictures were assigned to 2 or more specialists, and the double blind scan was used. The ultrasonographic findings of 60 patients with ketamine associated cystitis were recorded and compared with the pathological diagnosis. Results 60 cases of ketamine related cystitis were successfully examined with no interruption and withdrawal. The success rate of ultrasonography was 100.00％. The analysis showed that the coincidence rate of ultrasonography and pathological diagnosis was 60 (91.67％ and 8.33％) respectively, and there was no significant difference between the two groups. Conclusion the use of ultrasound has a detection rate of ketamine associated cystitis ideal, to provide a reliable basis for the diagnosis and treatment of ketamine associated cystitis doctor, has important value on the quality of life of patients with treatment effect and security.