1.Humanistic nursing of patients in the operation room
Chinese Journal of Practical Nursing 2012;28(30):8-9
Objective To investigate the influence of humanistic care on patients in the operation room.Methods 80 cases of surgery patients from November 2010 to October 2011 were given humanistic care,another 80 patients before implementation of humanistic care were chosen,and the satisfaction degree of doctors and patients and recovery of patients before and after the implementation of humanistic care were compared.Results After the implementation of humanistic care,the recovery of patients improved.The total satisfaction degree of patients and doctors reached 93.2% and 98.1%,significantly higher than those before the impIementation,which were 82.1% and 90.2%.Conclusions Implementation of humanistic care can improve the psychological and physical state of surgery patients,and help them gain better recovery.
2.Quantitative Evaluation of Endoscopy in Hospital Infection Management
Huai YANG ; Jinling YANG ; Hongling LI ; Yan XU ; Yuping LEI
Chinese Journal of Nosocomiology 2005;0(11):-
OBJECTIVE To explore the comprehensive endoscopic management to prevent hospital infection and reduce the incidence of infection in hospitals. METHODS According to the characteristics of the endoscopy,the quatitative evaluation of endoscopic examination management was included in the inspection and implementation system. RESULTS During quantitative evaluation in the past three years,there were no outbreaks of epidemics and the quantitative evaluation improved year by year. CONCLUSIONS Endoscopy management,leadership attention and support are the keys to ensure effective measures of co-management and quantitative evaluation.
3.Advance of Exercise Therapy for Frailty (review)
Yanan WANG ; Juanlan XU ; Hongling SONG ; Yujie GUO
Chinese Journal of Rehabilitation Theory and Practice 2017;23(5):558-562
This paper introduced the concept and prevalence of frailty, as well as the concept and mechanism of exercise therapy. For the frailty, there were various modes of exercise therapy, and several exercise prescriptions were reported.
4.Clinical Study on Treatment of Decreased Ovarian Reserve by Yishen Huayu Decoction
Yulan LIU ; Chunxia SONG ; Hongling BAO ; Hongyan XU
Chinese Journal of Information on Traditional Chinese Medicine 2017;24(3):30-33
Objective To observe the clinical efficacy of Yishen Huayu Decoction for decreased ovarian reserve (DOR). Methods Eighty cases of DOR were randomly divided into 2 groups, with 40 cases in each group. The treatment group was treated with oral administration of Yishen Huayu Decotion, one dose a day. The control group was given DHEA, a time of 1 tablet, 3 times a day. 2 groups were observed for 3 menstrual cycles. The levels of basic follicle stimulating hormone (bFSH), basal estradiol (bE2), luteinizing hormone (LH) and serum anti-Müllerian hormone (AMH) were measured. The number of basal follicles and the peak systolic velocity (PSV) were monitored by transvaginal sonography. Quality of life assessment and basal body temperature were measured. Results The total effective rate was 85.00% (34/40) in the treatment group and 62.50% (25/40) in the control group, with statistical significance (P<0.05). After treatment, the levels of bE2 and bFSH decreased, and the levels of AMH, ovarian antral follicles and PSV increased, which were significantly different from those before treatment (P<0.05). After treatment, the levels of bE2 and bFSH in the treatment group were lower than those in the control group, and the levels of AMH, ovarian antral follicle and PSV were higher than those in the control group, with statistical significance (P<0.05). The recovery rate of basal body temperature was 77.50%(31/40) in the treatment group and 52.50%(21/40) in the control group, with statistical significance (P<0.01). After treatment, the scores of four dimensions of life quality, daily activities, health status and self-perception were significantly improved (P<0.01), and the treatment group was higher than the control group (P<0.01). Conclution Yishen Huayu Decotion can improve the ovarian reserve function and the life quality of patients with DOR.
5.The significance of the monocyte human leukocyte antigen-DR level in the assessment of the severity of acute pancreatitis
Zhiping XU ; Hongling MA ; Shuangfeng CHEN ; Tiejun WU
Chinese Critical Care Medicine 2016;28(3):221-224
Objective To investigate the clinical value of the peripheral blood monocyte human leukocyte antigen-DR (mHLA-DR) for assessment of degree of severity and the diagnosis of acute pancreatitis (AP). Methods A case-control study was conducted. Eighty-six AP patients admitted to Shandong Liaocheng People's Hospital from June 2014 to May 2015 were enrolled. Patients were classified into four groups [mild (n = 33), moderate (n = 25), severe (n = 16), critical (n = 12)] according to the disease classification. Eighty healthy persons subjected to physical examination center of our hospital at the same time were served as controls. Acute physiology and chronic health evaluation Ⅱ (APACHE Ⅱ) scores in patients were estimated. Flow cytometry was used to measure the expression of the peripheral blood mHLA-DR, and the Pearson method was used to analyze the relationship between the level of mHLA-DR and the APACHE Ⅱ score. The receiver-operating characteristic curve (ROC) was plotted, and then the clinical value of the peripheral blood mHLA-DR was analyzed for the diagnostic value in AP patients. Results The expression of the mHLA-DR in patients with AP was significantly lower than that of healthy control group [(63.7±18.6)% vs. (86.4±8.3)%, t = 5.319, P < 0.001]. The expression levels of the mHLA-DR in mild group, moderate group, severe group, and critical group were (79.6±6.5)%, (66.4±9.4)%, (49.9±8.1)%, (32.5±12.0)%, respectively, and the APACHE Ⅱ score were 4.67±1.99, 5.88±2.05, 9.06±2.62, 12.33±3.96, respectively. Pair wise comparisons were statistically significant (all P < 0.05). The HLA-DR expression level in the peripheral blood of patients with AP was negatively correlated with the APACHE Ⅱ score (r = -0.695, P < 0.001). The area under the ROC curve (AUC) of mHLA-DR expression in peripheral blood for AP was 0.894 [95% confidence interval (95%CI) = 0.847-0.941, P < 0.001], and the cut-off point was 84.40%, with the sensitivity of 75.0%, the specificity of 90.7%, and the accuracy rate of 83.1%. The AUC of mHLA-DR expression for mild AP was 0.938 (95%CI = 0.889-0.987, P < 0.001), and the cut-off point was 72.70%, with the sensitivity of 87.9%, the specificity of 88.7%, and the accuracy rate of 88.4%. The AUC of mHLA-DR expression for severe and critical AP was 0.943 (95%CI = 0.881-1.005, P < 0.001), and the cut-off point was 57.85%, with the sensitivity of 84.0%, the specificity of 96.4%, and the accuracy rate of 90.6%. Conclusions The expression levels of the peripheral blood mHLA-DR in AP patients can reflect the degree of disease, and contribute to the diagnosis of AP. The value of mHLA-DR may be used as a new biological indicator in the diagnosis and assessment for the severity of AP.
6.Effects of genistein on proliferation and apoptosis of human non-small cell lung cancer cell line A549/DDP
Yan REN ; Hongling LU ; Yongxiang SONG ; Dayu LI ; Gang XU
Chinese Journal of Immunology 2014;(5):669-672
Objective:To observe the effects of genistein on proliferation and apoptosis of human non -small cell lung cancer cell line A549/DDP.Methods:①MTT assay was applied to evaluate the resistance index of A 549/DDP cell line to cisplatin and half in-hibitory concentration ( IC50 ) .②Inhibition rate of A549/DDP cell proliferation and IC 50 value were evaluated by MTT assay after treat-ment with 0, 1.25, 2.5, 5.0, 10, 20, 40, 60, 80 μg/ml genistein for 48 hour respectively.③A549/DDP cell cycle and apoptosis were evaluated by flow cytometry after treatment with 6.25, 12.5, 25 μg/ml genistein for 24 hours respectively.Results:①In expo-sing to cisplatin, the IC50 of A549 and A549/DDP was 33.6 μmol/L and 76.9 μmol/L respectively.The resistance index was 2.3. Cell growth inhibition rate increased following the cisplatin concentration increasing gradually .②A549/DDP growth inhibition rate in-creased at first and later decreased gradually following treatment with the genistein dose increased .The IC50 of A549 and A549/DDP was about 85.1 μg/ml and 80.2μg/ml respectively.③After treatment with 6.25, 12.5, 25μg/ml genistein for 24 hours, there were more A549/DDP cells arresting and showing apoptosis along with the genistein dose increased .Conclusion: Genistein can inhibit A549/DDP proliferation, cause A549/DDP arresting in G2/M phase and induce A549/DDP cell apoptosis with dose dependently .
7."Shaoyang for the pivot" is the core of "Shaoyang doctrine" theory
Rongqian XU ; Hongling WANG ; Hong ZHANG ; Junhong WANG ; Taoyu SUN
China Journal of Traditional Chinese Medicine and Pharmacy 2005;0(05):-
Since the children were born,their yin-yang balance in their body began.Children’s yin-yang balance is the yang taken a dominant role in yin-yang balance."Shaoyang for the pivot" is the core of "Shaoyang doctrine"theory.It is very proper to explain the children’s yin-yang balance with the state of continuous transporting and changing by the theory of "Shaoyang for the pivot".Along with "mutual promotion of growth between yin and yang",new yin yang balance continuously replace the old one,constructing children’s basic physical characteristic of "full of vigor and vitality,rapid development;viscera is delicate and organs function is not perfect".Meanwhile it is very proper to explain the pathological characteristics "easy incidence,rapid changing;viscera qi clean and full of spiritualism,easy rehabilitation".So "Shaoyang doctrine" can cover "pure yang doctrine" and "immature yin and yang theory".
8.Effect of diazoxide on change of H_2O_2 in rat pulmonary artery smooth muscle cells and proliferation of hypoxic rat pulmonary artery smooth muscle cells
Hongling HU ; Tao WANG ; Zhenxiang ZHANG ; Jianping ZHAO ; Yongjian XU
Chinese Journal of Pathophysiology 2000;0(10):-
AIM: To investigate the contribution of diazoxide,an opener of mitochondrial ATP-sensitive K+ channel(MitoKATP),and mitochondrial membrane potential(??m) to change of H2O2 in rat pulmonary artery smooth muscle cells(PASMCs) and to unbalance between cell proliferation and apoptosis of PASMCs induced by hypoxia.METHODS: The rat PASMCs were isolated from fresh normal lung tissues and cultured,which were divided into 6 groups,as follows: ① control group;② diazoxide group;③ 5-HD group;④chronic hypoxia group;⑤ chronic hypoxia+diazoxide group;⑥ chronic hypoxia +5-HD group.The relative change in mitochondrial potential was detected with rhodamine fluorescence(R-123) technique.The level of H2O2 in rat PASMCs was detected with chemiluminescence method.The proliferation of rat PASMCs was examined by cell cycle analysis and MTT colorimetric assay.RESULTS: After exposed to diazoxide for 24 h,the intensity of R-123 fluorescence,the level of H2O2 and the A value in normoxic rat PASMCs were significantly increased,and the apoptosis of rat PASMCs was significantly decreased as compared with control group(P
9.Combination of High Performance Liquid Chromatographywith Nano-electrospray ionization Chirp Z TransformIon Mobility Spectrometry
Hongling SHEN ; Qingyan MENG ; Xu JIA ; Wenjie LIU
Chinese Journal of Analytical Chemistry 2017;45(6):809-816
By combining frequency modulation Chirp Z transform ion mobility spectrometers (IMS) and multi nozzle electrospray array ionization source, a method of NanoESI-Chirp Z transform ion mobility spectrometry-high performance liquid chromatography was developed for the determination of n-alkyl ammonium bromide compounds.The parameters of NanoESI-Chirp Z transform IMS such as electric field intensity, solvent composition, and solution flow rate were investigated and optimized.Subsequently, four kinds of n-alkyl ammonium bromide compounds were respectively detected by this developed Chirp Z transform method and Fourier transform method, and the obtained results were compared.The result indicated that the optimum conditions were electric field intensity of 4.5 kV, and ESI solution flow rate of 8 μL/min.Then a test mixture containing tetrabutylammonium bromide, tetrapentylammoniumbromide, tetrahexylammonium bromide, tetraheptylammonium bromide, tetranoctylammoniumbromideandtetrakis(decyl) ammonium bromide was successfully separated and determined by the HPLC-nanoESI-Chirp Z IMS method.Chirp Z transform method provided higher signal to noise ratio compared to conventional signal averaging method, and was superior to FT method in the determination of drift time.
10.The application of wound care technologies combined with other multiple treatments in the hepatolithiasis patient who suffered from postoperative severe intestinal fistula
Hongling SUN ; Yan SUN ; Caiyun XU ; Jinping XIA ; Liangyu FANG
Chinese Journal of Practical Nursing 2021;37(16):1258-1261
Objective:To assess the nursing care experiences and curative effects of the application of wound care technologies combined with other multiple treatments in the hepatolithiasis patient who suffered from postoperative severe intestinal fistula.Methods:The wound care difficult points were analyzed,the wound care technologies combined with other multiple treatments including multiple debridement methods were applied according to the severity of the intestinal fistula:multiple debridement, new skin protection, continuous flushing and drainage with double cannula, improved negative pressure closure drainage and wound pull-in technology, combined with systemic treatment and nursing intervention.Results:The intestinal fistula was treated for 12 days, and the wound infection was controlled for 24 days. Finally the wound was healed after 43 days and patient was discharged from hospital.Conclusions:In the case of severe intestinal fistula after hepatolithiasis operation, the application of wound nursing technology combined with treatment scheme provides a new treatment idea and scheme for intestinal fistula and wound healing.