Objective:To evaluate the effects of domestic recombinant adenovirus p53 injection (rAd-p53,Gendicine) combined with cisplatin (DDP) on growth and apoptosis of ovarian carcinoma cell lines. Methods:MTT assay, flow cytometry, and Western blotting were used to compare the effects of rAd-p53, DDP and their combination on the growth, cell cycle, apoptotic rate, and p53 protein expression of ovarian carcinoma cell lines. Results:rAd-p53 inhibited ovarian carcinoma cells proliferation in dosE- and timE-dependent manners. After combined treatment, the growth inhibition rate was significantly increased (P<0.01). There was no significant diffE-rence in the inhibition potency induced by combined treatment with different sequences (P>0.05). Combined treatment significantly arrested ovarian carcinoma cell lines in G0/G1 phase at 72 h, the cell number in S phase was significantly decreased and the apototic rate was significantly increased (P<0.01). High expression of p53 protein was detected in ovarian carcinoma cell lines at 72 h after administeration of rAd-p53. Conclusion:rAd-p53 tranfects the exogenous widE-type p53 into the genome group of human ovarian tumor cells, induces p53 protein expression, thereby arresting tumor cells at G0/G1 phase, inhibiting tumor growth, and inducing apoptosis.

Objective To know the role of inhibitor of DNA binding 2(Id2) in the development of neural stem cells(NSCs) in the anterior subventricular zone(SVZa). Methods To examine the expression of Id2 in SVZa,rostral migratory stream(RMS) and olfactory bulb(OB) in E14,P0,P7,P14,P30 adult and old rat brains by reverse transcriptase-polymerase chain reaction(RT-PCR),Western blotting and immunohistochemistry. Results The expression of Id2 peaked at the area of RMS,and was less positive in SVZa and the least in OB at different time courses of developing rat brains.The expression of Id2 in the three areas in E14 were higher than that on the postnatal day,higher in OB and RMS in adult than that on P0,and remain high in old.However,the expression of Id2 in SVZa in adult was weaker than that on P0.Conclusion The expression of Id2 in the deveolpment of SVZa NSCs indicates that Id2 plays an important role in blocking the differentiation of SVZa NSCs and increasing the proliferation of SVZa NSCs.

Objective To observe the effects of parecoxib sodium on laparoscopic cholecystectomy ( LC ) patients after remifentanil fast track anesthesia.Methods 120 patients underwent general anesthesia for laparoscopic cholecystectomy were divided into two groups using random number table,60 cases in each group.The observation group was injected parecoxib sodium 40mg+saline solution 5 mL,while the control group was injected only saline so-lution 5mL prior to induction of anesthesia.The heart rate,mean arterial pressure,awaked time,pain score,agitation score and adverse reaction were compared.Results 5, 15, 30min after extubation, heart rate were ( 75.7 ± 4.8)times/min,(79.3 ±6.2)times/min,(74.2 ±5.2)times/min in the observation group and (85.2 ±3.8)times/min, (88.2 ±5.9)times/min,(86.1 ±6.2)times/min in the control group,and the mean arterial pressure were (103.5 ± 11.3)mmHg,(104.7 ±12.6)mmHg,(108.4 ±12.7)mmHg in the observation group and (132.3 ±16.3)mmHg, (135.2 ±14.6) mmHg,(133.6 ±11.8) mmHg in the control group,there were statistifically significant differences (t=4.722,4.357,4.236,4.732,4.367,4.063,all P <0.05).The pain score,agitation score were (2.5 ± 0.9)points,(1.4 ±0.8) points in the observation group,which were lower than (4.2 ±1.2) points,(3.2 ± 1.2)points in the control group(t =4.232,5.234,all P<0.05).Conclusion Parecoxib sodium can reduce the fast-track anesthesia agitation and pain,improve hemodynamics and stress response in LC patients.

Objective To investigate the relationship between homocysteine(Hcy)and blood uric acid(UA)in the patients with type 2 diabetes mellitus(T2DM)complicating hypertension.Methods 85 patients with T2DM complicating hypertension were se-lected as the groupⅠ,78 patients with simple T2DM as the group Ⅱ and 74 individuals with healthy physical examination as the group Ⅲ.Hcy,UA,TC,TG and HDL-C were measured in all the subjects.Results Compared with group Ⅲ(control),the levels of Hcy,UA,TC and TG in the group Ⅰand groupⅡwere significantly increased(P <0.05),but the level of HDL-C was significantly decreased(P <0.05).Compared with the group Ⅱ(T2DM),the levels of Hcy,UA,TC and TG in the group Ⅰwere significantly in-creased(P <0.05 ),but the level of HDL-C was obviously decreased,the difference had statistical significance (P <0.05 ).There was good positive correlation between the Hcy level and the UA level in the patients with T2DM complicating hypertension (r =0.658,P <0.05).Conclusion The levels of Hcy and UA are significantly increased in the patients with T2DM especially complica-ting hypertension.High Hcy and high UA are the important risk factors of T2DM complicating cardiocerebrovascular diseases.

Objective A meta-analysis was conducted to figure out the effects of anesthetic methods on postoperative myocardial infarction in noncardiac surgery patients.Methods We searched PubMed, Embase, Ovid, Cochrane Library, Google scholar, CNKI, Wang-fang data and VIP Database (by September 2016) to identify relevant studies that focused on the effect of intrathecal blockade on postoperative myocardial infarction and mortality in non-cardiac surgery.Meta-analysis was performed using software of RevMan 5.3.Results A total of 21 859 patients from 10 RCTs were enrolled.The meta-analysis showed that there were no differences in myocardial infarction within 7 postoperative days (OR=0.44, 95%CI 0.13-1.46, P=0.18) or 30 days (OR=1.49, 95%CI 0.89-2.49, P=0.13) and all-cause mortality (OR=1.26, 95%CI 0.84-1.88, P=0.26) between epidural anesthesia combined with general anesthesia and general anesthesia.Furthermore, there were no differences in myocardial infarction within postoperative 7 days (OR=1.14, 95%CI 0.31-4.17, P=0.84) and all-cause mortality within postoperative 30 days (OR=0.88, 95%CI 0.43-1.79, P=0.73) between spinal anesthesia alone and general anesthesia alone.Conclusion Intrathecal blockade cannot affect the incidence of myocardial infarction and mortality in high-risk cardiac patients undergoing intermediate or high-risk non-cardiac surgery.

Objective To investigate the changes of the expression of GDNF and GDNFR?1 of long-term denervation of posterior cricoarytenoid muscles(PCAMs).Methods 38 patients with vocal paralysis were grouped into four according to their denervated period of time while 12 normal PCAMs as control group.Using double immunofluorescence stain,changes of GDNF and GDNFR1 expression were observed in myofibers at different time points after denervation.Results Double immunofluorescence stain with antibodies against GDNF and GDNFR1 showed no staining in the control group,and study groups.However,after the muscle denervation lasted for 6-12 months and 1-2 years,noted was a significant accumulation of GDNF and GDNFR1 protein in cytolemma and endochylema of myofiber.The mean grey scales and positive region ratios were compared using the image analysis system.The results revealed the levels of GDNF and GDNFR1 protein expression in 6-12 months group,1-2yr group changed significantly(P0.05).Conclusion The changes in expression of GDNF and the acceptor GDNFR-?1,a powerful neurotrophic factor,implied that a good nervous regenerated microenvironment in PCAMs within 2 years.This experiment indicated that denervated posterior cricoarytenoid muscles are able to regain their functions through reinneration within 2 years.

Objective To investigate the effect of diosmin on the pain and wound healing after modified anal fissure resection .Methods 98 patients from January 2015 to July 2016 in our hospital who were admitted to meet the inclusion criteria and improved anal fissure resection patients were selected and randomly divided into control group and study group, 49 cases in each group.And the control group was given routine postoperative treatment, the study group was treated with diosmin tablets on routine postoperative therapy , two groups were treated for seven days for a course of treatment, the wound pain, wound width and wound healing were observed and recorded before and after treatment, blood samples were taken to determine changes in serum inflammatory factors, the clinical efficacy and complications were compared.Results After treatment, the two groups of wound pain, the width of the wound and wound healing rate were decreased, the serum TNF-α, IFN-γ, IL-6 levels were decreased, the difference was statistically significant (P<0.05), compared with the control group, the degree of pain, the width of the wound and wound healing rate were lower, the serum TNF-α, IFN-γ, IL-6 content were lower, the average healing time and granulation growth time and remove decay time were shorter, the difference was statistically significant (P<0.05).The effective rate of the study group was 91.83%, higher than the control group 75.51%, the difference was statistically significant (P<0.05).All patients were followed up, no case falls off phenomenon, there was no significant difference in the incidence of adverse reactions between the two groups.Conclusion Diosmin tablets on the improvement of anal fissure after resection of wound healing has a role in promoting inflammation, reduce the severity of pain conditions.

Objective The research is to study the temperature rising situation in the thermochemotherapy process of the domestic dog through the hepatic artery, and the bearing ability of heart, liver, and kidney during the process, as well as the biochemical and histological change of the liver after the procedure. Methods A thermostated container of water bath and an infusion pump, both designed domestically, were adopted. Ten domestic dogs were divided into 2 groups of A and B with 5 dogs respectively. Through hepatic artery infusion for group A, glucose solution (GS) was administered at 43?C for 30 minutes, with the flowing rate of 30ml/min, in group B 5% GS at 45?C for 30 minutes, with the flowing rate of 40ml/min added with chemotherapy drug MMC(0.2mg/kg) was administered to 3 dogs and 5% GS with the same flowing rate added with CDDP(1mg/kg) into other 2 dogs. Results The temperature of anus and liver was 0.3～0.5?C and 0.5～1.0?C higher than before the procedure respectively, there was no significant rate changes of heart and respiration during and before the procedure. Four dogs were radiographied after 15 minutes' infusion, showing different degrees of hepatic artery dilation accompanied with relevant increase of infusion. Six dogs were radiographied after 30 minutes' infusion, 5 of them showed arterial contraction astescal on accompanied with relevant decrease of infusion. There appeared slight or moderate increase of aminopherase after the procedure, but in actually returned to normal within a week. Pathologically, there appeared no acute effect on heart, lung and kidney other than slight and reversible damage to hepatic tissue. Conclusions Healthy dogs can endure the chemotherapy treatment of infusion through hepatic arterial fistula at 43?C 45?C for 30 minutes.

Objective To evaluate the clinical efficiency of uterine artory embolization for uterine fibroids. Methods From August 1999 to June 2001, 405 cases were included in the treatment by using UAE and were followed up to find its efficiency. Results The successful rate of catheterization almost reached 99.8% (2/405). B mode ultrasound examination following 3～12 months after the procedure re vealed an average shrinkage of 40%～60% in volume of the masses for all cases in the first 3 months and 50%～70% in volume in 6 months, with 22 UF disappeared together with conspicuous decrease in volume of menstruation. Conclusions UAE for UF is a new, safe and effective method

Degeneration of the lumbar facet joints is considered to be one of the main causes of low back pain,which severely impact the life quality of people.This article sums up the research advancement of the lumbar facet joints degeneration of domestic and foreign scholars in recent years,and summarizes the characteristics of the X-ray,CT and MRI and clinical significance of the degeneration of articular process,of which the characteristic of MRI was rarely reported domesticly.The purpose is to provide ideas and references for domestic scholars aiming to further clinical research of related fields in the future.