Objective To study the surgical techniques with keyhole operation for treatment of the huge intracranial meningioma located in the frontal skull.Methods 39 cases of huge intracranial meningioma were removed by microsurgery with supra-eyebrow approach keyhole operation.Results 39 cases of meningioma were totally removed.There was no associated morbidity,mortality or exacebation in all cases.Conclusion It suggests that keyhole microsurgery may provide maximum efficiency to remove the huge intracranial tumor located in the frontal skull and minimum invasiveness.However,it is very important to choose indication properly,to prepare sufficienctly before operation,and to operate with proficient skill of microsurgery.

Objective To evaluate the efficacy and toxicity of hyperthermia combined with chemoradiotherapy for local advanced cervical cancer. Methods Sixty-eight patients with local advanced cervical cancer were treated. Each patient was given cisplatin at the dose of 20 mg ivgtt dl -2 and 5 -fluorouracil 0.75 ivgtt dl -2, repeating every week, total 3 weeks. Thirty -eight patients received chemoradiotherapy alone. Thirty patients received hyperthermia with deeply focus technology, the temperature was increased and maintained 42.5 ℃ for 30 min at least, within 2 hours following irridiation. Hyperthermia was given twice per week with 7 or 12 sessions, as the same chemoradiation methods as the reference group.After 40 Gy therapy to estimate by cervical examination and ultrasonography, the patients with down stage were for operation, and the others continued to irridiation and additional brachytherapy with californium 252until radical dose. Results All patients were tolerated, fifteen patients of hyperthermia combined group were operated and ten patients of the reference group were operated, the difference was significance (50.0 % vs.26.3 %, P <0.05). The response rate of the combined group was 90.0 %, and the reference group was 84.2 %.The incidence of radiotherapy toxicity of the combined group was not higher than the reference group.Conclusion Hyperthermia combined with chemoradiotherapy can elevate the resection rate and the local control rate, not increase the radiotherapy toxicity and increase quality of life for cervical cancer patients.

Acetylation regulated by histone deacetylases (HDAC) has a broad influence on plenty of physiological processes and regulation of malignant tumor.HDAC inhibitors can promote tumor cell apoptosis and have little effect on normal cells,so they have been developed a new kind of anti-tumor agent,and part of them have entered clinical trials.Vorinostat and romidepsin have been approved by FDA for treating cutaneous T cell lymphoma patients with progressive,persistent and recurrent disease.Studies of the molecular mechanisms of the HDAC inhibitors will contribute to the further clinical application.

OBJECTIVE:To study the anti-hepatic fibrosis effects of Yidu tiaogan mixture on rats and its mechanism. METH-ODS:60 SD rats were randomly divided into normal group,model group,colchicine group(positive control,0.2 mg/kg)and Yi-du tiaogan mixture low-dose,medium-dose and high-dose groups(2.7,5.4,10.8 mL/kg). Except for normal group,liver fibrosis model was induced in other groups,and they were given relevant medicine intragastrically during modeling,once a day,for 8 weeks. Serum levels of ALT,AST,HA,PCⅢ,CⅣ,TIMP-1,MMP-1,MMP-2,MMP-13,TGF-β1 and TNF-α and the levels of Hyp,SOD,GSH-Px,CAT,MDA,TGF-β1 and TNF-α in liver tissue were detected in rats. RESULTS:Compared with normal group,the serum levels of ALT,AST,HA,PCⅢ,CⅣ,TIMP-1,MMP-2,TGF-β1 and TNF-α were increased significantly in model group,while the serum levels of MMP-1 and MMP-13 were decreased significantly(P<0.05);the levels of Hyp,TGF-β1, TNF-α and MDA in liver tissue were increased significantly,while the levels of SOD,GSH-Px and CAT were decreased signifi-cantly(P<0.05). Compared with model group,the serum levels of ALT,AST,HA,PCⅢ,CⅣ,TIMP-1 and TNF-α as well as the levels of Hyp,TGF-β1,TNF-α and MDA in liver tissue were all decreased significantly of rats in Yidu tiaogan mixture medi-um-dose,high-dose and colchicine groups(P<0.05);the serum levels of MMP-1 and MMP-13 as well as the level of CAT in liver tissue were increased significantly in Yidu tiaogan mixture high-dose group and colchicine group(P<0.05),while the serum levels of MMP-2 and TGF-β1 were decreased significantly(P<0.05). There was no statistical significance in above indexes in Yidu tiao-gan mixture low-dose group (P>0.05). CONCLUSIONS:Yidu tiaogan mixture exerts protective effects on liver fibrosis of rat through regulating TIMP/MMP balance,suppressing oxidative stress,decreasing the level of TGF-β1 and reducing deposition of ex-tracellular matrix in liver.

Objective To study the influence of continous ven ovenuous hemofiltration on patients with multiple organ dysfunction syndrome.Methods 64 patients with MODS were divided into CVVH group(34) and IHD group(30)between 1999-12~2003-12.The serum BUN,Cr,iron,blood pressure,ar rhythmia were monitored during treatment and KT/V was also calculated after each therapy.APACHE-Ⅱ score was assessed before and after the treatment and the pr ognosis of each patient was recorded as well.Results CVVH group had more stable hemodynamics than IHD group; there was no significant difference in the removal of BUN and Cr in the two grou ps.The survival time of CVVH group was longer than IHD,and APACHE-Ⅱ score of C VVH group was lower than that in IHD group,17.23?6.86 verse 20.91?5.77 ( P

Objective To investigate the role of ERK1/2 signal pathway in the apoptosis of rat mesangial cells inhibited by TIMP-1.Methods The human TIMP-1 sense and antisense plasmid were transferred into rat mesangial cells through liposome,while mesangial cells transfected with pcDNA3 and non-transfected mesangial cells were used as control.After being stimulated with high glucose(HG 25mmol/L D-glucose)and PD98059(50 ?mol/L)for 24 hours,the apoptosis rate was measured by flow cytometry.The expression of ERK1mRNA was assayed by RT-PCR.The protein expression of p-ERK1/2 in the cell was analyed by ELISA.Results(1)The apoptosis rates of the non-transfected group,transfect pcDNA3 group,sense group and antisense group in the culture medium of high glucose withact PD98059,were(12.10?2.21)%,(11.90?3.34)%,(5.50?0.50)%and(20.70?3.41)%,respectively.The apoptosis rate of the sense group was significantly lower than that without transfection(P

Objective To determine and compare the contents of free total organic acids in Pinellia ternata and its processed products.Methods Back potentiometric titration was employed to determine the contents of free total organic acids in Pinellia ternata and its processed products.Results The contents of free total organic acids in three batches of Pinellia ternata and its processed products were determined.The contents of free total organic acids in prepared rhizoma pinelliae was higher than that of the crude drug.The recovery was 95.20% and RSD was 1.81%.Conclusion This method were simple,accurate and can be used to estimate the quality of Pinellia ternata and its processed products(prepared with potash alum and juice of rhizima zingiber or with just potash alum).

OBJECTIVE To investigate the clinical effect of Ashi Dispel-itch solution on treatment of diabetic-candidal vaginitis.METHODS Totally 322 patients were randomly divided into 2 groups,one group was treated with Ashi Dispel-itch solution by fumigating and soaking ill section while the control group with Jieyin solution.The curative effect was divided into 4 grades according to symptoms,body appearance,level of pH,etc.RESULTS The total effective rate due to Ashi Dispel-itch solution was 93.5% and was significantly higher than that of Jieyin solution(P

Objective To investigate the association between a 27-bp variable number of tandem repeat(VNTR) polymorphism of the endothetial nitric-oxide synthase(eNOS)gene and transient ischemic attack(TIA) of brain.Methods Polymerase chain reaction was performed for the genotypes of all patients and control subjects.Data were compared between patients and control subjects using ?~(2)test. Results The genotype ab distribution and allele a frequencies of eNOS gene were significantly higher in TIA group(25.0%,14.3%) than in control group(16.3%,9.2%)(P

Objective To analyze the factors influencing augmentation index (AI) by examining the AI changes in patients who received continuous ambulatory peritoneal dialysis (CAPD) therapy, and evaluate the artery elastic function situation. Methods The height, weight, blood pressure, blood indicators were detected in 46 CAPD patients (CAPD group) and 42 controls (control group). AI was managed by OMRON HEM-90OOAI, and the influencing factor was analyzed. Results Systolic blood pressure(SBP),diastolic blood pressure(DBP), pulse pressure, central aortic pressure and AIp75 (AI value, when pulse was 75 beats/min) in CAPD group were significantly higher than those in control group. Within CAPD patients,the AIp75, in female was significantly higher than that in nude [(96.67±8.95 )% vs (89.89±6.46)% ,P =0.022]. Simple correlation showed positive relationship in gender, age, SBP, DBP, pulse pressure and central aortic pressure with AI; and negative relationship in height, weight and weight index with AI; while there was no relationship between AI and all kinds of chemical examination indicators or dialysis years.Multiple linear regression analysis evidenced that gender,age, height, DBP and central aortic pressure of CAPD patients had linear regression relation with AI. Conclusions It indicates a decreased artery elasticity and an increased stiffness occurs in CAPD patients. AI has tight correlation with age, height, DBP and central aortic pressure, and is an non-invasive factor thai partially reflects the arterial elasticity. Central aortic pressure is relevant with aortic elasticity and rigidity closely. Measuring central aortic pressure is very imporrant for preventing cardiovascular accident.