1.Mechanism of mesenchymal stromal cells in promoting islet graft revascularization
Rui LI ; Hongli DONG ; Baolin LIU
Chinese Journal of Hepatobiliary Surgery 2017;23(7):474-477
Objective To investigate the mechanisms of mesenchymal stromal cells (MSCs) in promoting the transplanted islets neovascularization.Methods We performed co-transplantation of allogeneic islet and MSCs into non-obese diabetic (NOD) mice and the lymphocyte chimeric rate was detected by flow cytometry.The MSCs migration,transformation and secretion of cytokines were detected by immunofluorescence.Results In mice of co-combination transplantation of islet with MSCs,donor lymphocytic chimerism in graft was increased.BM-MSCs can migrate to transplanted islet,differentiate into vascular smooth muscle cells,and also secrete vascular endothelial growth factor (VEGF).Conclusions MSCs can migrate to transplanted islet and promote neovascularization.Also,it enhanced allograft immune tolerance of islet grafts via increasing donor lymphocytic chimerism.
2.Open-window bone grafting combined with internal fixation for the treatment of inter-articular calcaneal fractures.
China Journal of Orthopaedics and Traumatology 2009;22(9):714-715
Adult
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Calcaneus
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injuries
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surgery
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Female
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Fracture Fixation, Internal
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methods
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Fractures, Bone
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diagnostic imaging
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surgery
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Humans
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Male
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Middle Aged
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Radiography
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Treatment Outcome
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Young Adult
3. Effects of hydrotherapy with traditional Chinese medicine and magnetotherapy on treatment of scars after healing of deep partial-thickness burn wounds in children
Rui LIU ; Weihong CAO ; Hongli LIU ; Dandan ZHANG
Chinese Journal of Burns 2018;34(8):516-521
Objective:
To study the effects of hydrotherapy with traditional Chinese medicine combined with magnetotherapy on treatment of scars after healing of deep partial-thickness burn wounds in children.
Methods:
Forty-eight children with scars after healing of deep partial-thickness burn wounds admitted to the Burn Department of Air Force General Hospital from January to December in 2016 met the criteria for inclusion in this study, and their medical records were analyzed retrospectively. Within one month after the wound was completely healed, 24 children were treated with silicone cream for 2 times per day and wore elastic sleeves all day long according to the wishes of the children and their families, and they were enrolled in routine treatment group. The other 24 children were treated by hydrotherapy with traditional Chinese medicine Fuchunsan No. 2 once every 2 days for 30 min each time on the basis of treatment methods of routine treatment group, plus magnetotherapy using pulse magnetotherapy apparatus in the morning and evening of each day for 30-60 min each time, and they were enrolled in hydrotherapy+ magnetotherapy group. Before treatment and 12 weeks after treatment, the Vancouver Scar Scale was used to assess the scar condition of children, the Wong-Baker Facial Expression Scale was used to assess the degree of scar pain of children, the Numerical Rating Scale was used to assess the degree of scar itching of children, the modified Barthel Index was used to assess the activity of daily living (ADL) of children, and the Motor Function Evaluation Scale was used to assess the dysfunction condition of limbs with scar. After 12 weeks of treatment, the incidence of deformity of children was calculated. Data were processed with
4.Analysis of the maintenance treatment and prognostic factors of metastatic colorectal cancer patients with stable disease symptoms after induction chemotherapy
Xingyun CHEN ; Likun ZHOU ; Xia WANG ; Ming BAI ; Rui LIU ; Hongli LI ; Yi BA ; Dingzhi HUANG
Chinese Journal of Clinical Oncology 2016;(2):76-80
Objective: To investigate the survival and individualized therapy of metastatic colorectal cancer (mCRC) patients who achieved a stable disease state after induction chemotherapy. Methods:Data were reviewed from 204 metastatic colorectal cancer pa-tients, who presented a stable disease state after first-line and second-line chemotherapy at Tianjin Medical University Cancer Insti-tute and Hospital. The clinical and pathological characteristics were analyzed. Moreover, we analyzed the significance of maintenance treatment in patients with certain mCRC characteristics. Results:Univariate analysis indicated that the line of chemotherapy, levels of CA724, CEA, and CA19-9, and platelet-to-lymphocyte ratio (PLR) were considered prognostic factors of treatment after induction che-motherapy. According to the multivariate analysis, first-line chemotherapy, as well as low levels of CA19-9 and PLR, with maintenance treatment after the induction chemotherapy was significantly associated with better survival. Among the patients with high levels of PLR, those who underwent maintenance treatment achieved a progression-free survival of 13.43 months (versus 10.63 months in pa-tients from the observation group, P=0.003). Conclusion:The levels of CA19-9 and PLR, and treatment after chemotherapy were signif-icant prognostic factors for mCRC patients who achieved a stable disease state after induction chemotherapy. These patients, especial-ly those with high PLR, could benefit from the maintenance treatment.
5.Diagnostic value of multi-slice computed tomography in patients with incisional hernia before operation
Qingdong YAO ; Xiaojie CHENG ; Chengbing ZHANG ; Dongyou ZHANG ; Hongli ZHOU ; Rui HAN ; Chongyong XU
Journal of Practical Radiology 2015;(8):1294-1296,1300
Objective To evaluate the diagnostic value of multi-slice computed tomography (MSCT)for incisional hernia of ab-dominal wall before operations.Methods Thirty patients with incisional hernia in abdominal wall confirmed by the pathology were enrolled in this study.All patients underwent MSCT examination with sagittal and coronal MPRs,and 21 cases underwent ultra-sound (US)examinations.The value of transverse scan and MPRs & MSCT and US on demonstrating the number,location,con-tents of incisional hernia,and accompanying intestinal obstruction or other complications were retrospectively evaluated and com-paired.Results Of 30 cases,there were 40 hernias,the rate of MSCT and US in detecting incisional hernias were 97.5% and 56.0%, respectively.MSCT performed better than US on demonstrating the incisional hernias (P =0.000).On MSCT,there was no signif-icant difference in the detection for isolated incisional hernia between the MPRs and axial scans (P = 1.000 ).However,MPR showed a more accurate rate for two or multiple incisional hernias diagnosis (P =0.006),and the sagittal reconstruction displayed more correct than coronal reconstruction on two or multiple incisional hernias (P =0.01 9).Furthermore,MSCT can exactly reveal the complications of incisional hernias (7 cases of intestinal obstruction,5 cases of intestinal ischemia and edema,and 1 case of me-tastasis in incisional hernia),which were not detected by US.Conclusion MSCT can correctly demonstrate the incisional hernia of abdominal wall and its complication,it can provide important imaging reference for clinical therapy.
6.Carrier detection and prenatal diagnosis of hemophilia Alpha.
Yuanfang LIU ; Xuefeng WANG ; Haiyan CHU ; Zhiguang LI ; Hongli WANG ; Zhenyi WANG
Chinese Medical Journal 2002;115(7):991-994
OBJECTIVETo establish an effective laboratory examination system for carrier detection and prenatal diagnosis of haemophilia Alpha(HA) with a variety of molecular biological methods which are simple,rapid and easy to use.
METHODSDetection of inversion involving intron 22 in the FVIII gene was completed by long distance polymerase chain reaction (PCR) and linkage analysis was performed by using several genetic polymorphisms including an intragenic Bcl I RFLP, 2 STRs and an extragenic St14 VNTR.
RESULTSIntron 22 inversion was observed in 10 out of the 21 (47.6%) pedigrees examined. Prenatal diagnosis was completed in 3 pedigrees. A further combination of the four intragenic and extragenic polymorphic loci gave an informative rate of 94.7%.
CONCLUSIONSFemale relatives in HA families with inversion can be detected with direct diagnostic procedure. The application of long distance PCR makes the detection much more simple and rapid. For families without inversions,it is easier and more cost-effective to undertake linkage analysis of genetic polymorphism based on PCR.
Chromosome Inversion ; Female ; Genetic Carrier Screening ; Hemophilia A ; diagnosis ; genetics ; Humans ; Introns ; Minisatellite Repeats ; Polymorphism, Restriction Fragment Length ; Pregnancy ; Prenatal Diagnosis
7.Effect of bone mesenchymal stem cell transplantation on accelerating the vascularization surrounding transplant pancreatic islet
Rui LI ; Hongli DONG ; Rubin LIU ; Baolin LIU
Organ Transplantation 2017;8(2):149-153,160
Objective To investigate whether pancreatic islet transplantation in combination with bone mesenchymal stem cells (MSC) transplantation can promote the vascularization surrounding the transplant pancreatic islet.Methods The non-obese diabetic (NOD) mice were utilized as the recipients and randomly divided into pancreatic islet transplantation combined with MSC transplantation group (co-transplantation group,n=6),pancreatic islet transplantation group(n=6),MSC transplantation group(n=6) and sham transplantation group (n=3).The variation in blood glucose level and survival rate post-transplantation of NOD mice in each group was observed.The proliferation and apoptosis of the transplant pancreatic islet in the pancreatic islet transplantation group and co-transplantation group at 1,2 and 4 weeks after pancreatic islet transplantation were analyzed by 5-ethynyl-2'-deoxyufidine (EdU) and terminal deoxynucleotidyl transferase-mediated dUTP nick end labeling (TUNEL).The vascular density surrounding the transplanted pancreatic islet in the pancreatic islet transplantation group and co-transplantation group at postoperative 2,4 and 8 weeks were observed under light microscope and quantitatively analyzed by histochemical and immunohistochemical staining.Results Both MSC combined with pancreatic islet transplantation and pancreatic islet transplantation significantly improved the blood glucose level and enhanced the survival rate of NOD mouse models after transplantation.In addition,it could accelerate the regeneration of pancreatic islet cells and decrease cell apoptosis.MSC combined with pancreatic islet transplantation significantly enhanced the vascular density surrounding the transplant pancreatic islet compared with pancreatic islet transplantation alone.Conclusions MSC transplantation can accelerate the vascularization surrounding the transplant pancreatic islet,increase the blood supply and protect the function and activity of the transplant pancreatic islet.
8.Study of isolation efficiency of new islet isolation solution for mouse islets
Rui LI ; Hongli DONG ; Baolin LIU
Organ Transplantation 2018;9(3):188-193
Objective To investigate the isolation and protective effect of a new islet purification solution (IPS)-Optiprep solution on the islet in mouse models. Methods The digested pancreatic islets were divided into the IPS and UW groups according to the islet volume. The pancreatic islets were isolated by the continuous gradient density centrifugation using IPS-Optiprep or UW-Optiprep solutions. The purification efficiency and isolated islet activity of purification solution were compared between two groups. The diabetic mouse models were successfully induced and randomly assigned into three groups. In the experimental group (n=10), the mice received pancreatic islet transplantation using islets isolated and purified by the IPS-Optiprep solution. In the control group (n=10), the mice underwent pancreatic islet transplantation using islets isolated and purified by the UW-Optiprep solution. In the sham surgery group (n=5), the mice merely underwent surgery without pancreatic islet transplantation. Postoperative blood glucose levels were detected and compared among three groups. The blood glucose levels of intraperitoneal glucose tolerance test at postoperative 21 d were statistically compared between the experimental and control groups. The cost of the preparation of two isolation solutions was also compared. Results Compared with the UW group, the islet equivalent (IEQ), islet purity, recovery rate and islet integrity were significantly higher in the IPS group. Islet morphological observation revealed that the islet membrane was complete and the islet diameter in the IPS group was considerably larger than that in the UW group. The activity of purified islets in the UW group was significantly higher than that in the IPS group [(88±5)% vs. (84±3)%, P<0.01]. Compared with the UW-Optiprep solution, identical in vivo islet function was obtained in the IPS-Optiprep solution.The cost of IPS-Optiprep solution was significantly less than that of the UW-Optiprep solution. Conclusions The new IPS-Optiprep solution yields higher islet isolation efficiency, purification, integrity and recovery rate and significantly reduces the purification cost compared with the UW-Optiprep solution. Nevertheless, IPS-Optiprep solution exerts a less protective effect on the activity of islet cells, which is probably correlated with the high islet integrity and the endotoxin in the IPS-Optiprep solution.
9.Development strategies on seed seedling of Chinese medicinal materials.
Longyun LI ; Rui PENG ; Hongli LI ; Yekuan WU ; Guanglin CUI
China Journal of Chinese Materia Medica 2010;35(2):247-252
This paper analyzed the development status, industrialization system, standardization system and new variety breeding of seed and seedling of Chinese medicinal materials. Based on the development status and problems facing to the seed and seedling industry in China, some reasonable countermeasures are proposed in this article. The main measures include strengthening new variety breeding, establishing experiment site, protecting new variety and standardizing the market and quality management.
Agriculture
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legislation & jurisprudence
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organization & administration
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trends
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Breeding
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China
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Conservation of Natural Resources
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legislation & jurisprudence
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trends
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Plants, Medicinal
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chemistry
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genetics
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Quality Control
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Seedlings
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chemistry
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genetics
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Seeds
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chemistry
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genetics
10.Performance of modified Hodge test on the detection of carbapenemase among Enterobacteriaceae
Qiwen YANG ; Rui ZHENG ; Hui WANG ; Minjun CHEN ; Yingchun XU ; Weiyuan WU ; Yunsong YU ; Ziyong SUN ; Mingqing TONG ; Rong ZHANG ; Kang LIAO ; Bin CAO ; Xinhong HUANG ; Lianna ZHU ; Yuxing NI ; Ping JI ; Chao ZHUO ; Danhong SU ; Hong FAN ; Wenen LIU ; Xiuli XU ; Hongli SUN ; Xiuli XIE
Chinese Journal of Laboratory Medicine 2010;33(12):1122-1127
Objective To evaluate the performance of modified Hodge test on the detection of carbapenemases among Enterobacteriaceae with decreased susceptibility to carbapenems. Methods Fortynine Enterobacteriaceae isolates with decreased susceptibility to carbapenems ( MIC of imipenem, meropenem or ertapenem was ≥ 2 μg/ml ) were collected from 16 teaching hospitals from 2004 to 2008. MICs of imipenem, meropenem and etapenem were determined by agar dilution method. Carbapenemases were detected by modified Hodge test. Carbepenemase-causing positive results and AmpCs-causing positive results were differentiated by phenyl boronic acid and oxacillin. Beta-lactamases encoding genes including blaNDM-1were detected by PCR and sequencing. Results Thirty-six of 49 isolates were non-susceptible to imipenem (MIC >4 μg/ml), 31 were non-susceptible to meropenem (MIC > 4 μg/ml) and 47 were non-susceptible to ertapenem (MIC > 2 μg/ml). Twenty-three isolates showed positive modified Hodge test result, including 9 weak-positive results and 14 strong-positive results. Through PCR detection and sequencing, 2 out of 9 isolates showing weak-positive results carried blaKPC-2 and other 7 did not carry any carbapenemase genes but AmpCs/ESBLs genes. Among the 14 isolates showing strong-positive results, 4 carried blaKPC-2, 8 carried blaIMP-4 and 2 caried blaIMP-8. All 26 isolates with negative modified Hodge test result didn't carry any carbapenemase genes. No isolate carried blaNDM-1. Carbapenemases genes PCR detection was regarded as a gold standard, and the sensitivity, specificity, positive predictive value and negative predictive value of modified hodge test was 100%, 79%, 70% and 100% on the detection of carbapenemases among Enterobacteriaceae with decreased susceptibility to carbapenems. Conclusions Modified Hodge test revealed great sensitivity but showed a few false positive results. True and false positive results can be effectively differentiated by phynel boronic acid and oxacillin.