Humans ; PPAR gamma ; Plaque, Atherosclerotic

OBJECTIVE:To explore optimal regime and less ADR for lupus nephritis(LN) by adopting self-made TCM prescription.METHODS:109 LN patients were assigned into control group(n=48) and therapy group(n=61).Both groups were treated with metacortandracin and cyclophosphamide.Control group were additionally given self-made TCM prescription.The clinical efficacy and adverse drug reaction(ADR) of 2 groups were compared.RESULTS:The curative rate and total response rate of therapy group were 77.05%(47/61) and 95.08%(58/61) respectively and those of control group 58.33%(28/48) and 77.08%(37/48).The curative rate and total response rate of therapy group were all higher than those of control group.There were significant difference between 2 groups(P

OBJECTIVE To investigate the ?-lactamases producing rate in Haemophilus influenzae(HIN) and the antibiotics sensitivity,to guide the treatment of Haemophilus species infection.METHODS The strains from respiratory tract samples,were isolated and cultivated in chocolate cultures 2006 to 2007.Drug sensitivity test and beta-lactam enzyme test of Haemophilus species were taken.The results were analyzed by software SPSS11.5.RESULTS Totally 195 isolates of HIN were isolated 32 produced ?-lactamases(16.4%).The sensitivity of H.influenzae to cefuroxime,cefotaxime and ampicillin/sulbactam was 90.8%,93.8% and 95.4%,respectively.CONCLUSIONS Cefotaxime,ampicillin/sulbactam and cefuroxime are the most effective antibiotics for H.influenzae infection.

Pulmonary arterial hypertension (PAH) is an incurable fatal disease, with the mechanism of imbalance of vascular contraction and relaxation which start as a series of pathophysiological reactions. Angiotensin-converting enzyme 2-angiotensin (1-7)-G protein-coupled receptor axis [ACE2-Ang(1-7)-Mas], an expanded vascular axis which is considered as new axis of renin-angiotensin system (RAS) is a negative regulator of the classical angiotensin-converting enzyme-angiotensin Ⅱ-angiotensin Ⅱ type 1 receptor axis (ACE-AngⅡ-AT1R), a contraction vessel, proliferation axis. A retrospective study concerning the pathophysiological mechanism of ACE2 in the treatment of PAH was done and found that ACE2-Ang-(1-7)-Mas axis could reduce PAH induced by monocrotaline (MCT) through declining the inflammatory cascade, improving endothelial dysfunction and regulating autonomic nerve, reduce PAH induced by hypoxia through inhibiting the proliferation of pulmonary artery smooth muscle cells and reduce PAH induced by congenital heart disease. Therefore, ACE2 may become a future drug of preventing and treating PAH.

Objective To investigate the serum lipoprotein (Lp) (a) level in patients with acute ischemic stroke and the prediction for short-term functional outcome. Methods The levels of serum Lp(a) were measured in 210 patients with acute ischemic stroke (within 24 h after onset) and 100 healthy people matched the gender and age. The patients were assessed with National Institutes of Health Stroke Scale (NIHSS) as admission, and modified Rankin Scale (mRS) as discharge. Results The median of Lp(a) was 325 mg/L (IQR: 180~545) in patients,significantly more than 148 mg/L (IQR: 69~265) in the normal controls (P<0.001). Level of serum Lp(a) was an independent variable for functional outcome (OR=1.004, 95%CI: 1.001~1.007, P<0.001) and death (OR=1.006, 95%CI: 1.001~1.008, P<0.001) in patients. In the receiver operating characteristic curve, the area under curve was more in the combined model [Lp(a) and NIHSS] than the NIHSS alone.Conclusion Serum Lp(a) increased in the in patients with acute ischemic stroke, and may predict the short-term outcome independently.

Objective To optimize the extraction conditions of Hyperici Perforate Herba, Coptidis Rhizoma, Schisandrae Chinensis Fructus, and Ziziphi Spinosae Semen forJinzao Yuanzhi Capsule.Methods Hyperin extraction rate was set as index, and HPLC was used for determination. The extraction conditions of ethanol concentration, ethanol quantity, extraction time and other factors were studied with orthogonal test and comparative test.Results Ethanol concentration and extracting times were remarkable factors in the test. The optimum extraction technology forJinzao Yuanzhi Capsule was to reflux and extract the materials with 8 times of 70% ethanol for 2 times, reflux extraction for 1.5 hours, filtration. Then 6 times of 70% ethanol was added for 1.5 hours’ reflux, filtering, and merging extracted liquid.Conclusion The optimum extraction technology is reasonable and feasible.

Objective]To investigate the effect of acupuncture-assisted anesthesia on cerebral state index(CSI) under the steady-state anesthesia of sevoflurane. [Methods] Fifty patients scheduled for elective knee arthroscopy operation were randomly divided into two groups with 25 in each. All patients were induced with sevoflurane, the end-tidal concentrations of sevoflurane in two groups were adjusted to 1.0 MAC and maintained for 15 min after tracheal intubation, acupuncture-assisted anesthesia was used on the affected side in group A, Yanglingquan(GB34) and Zusanli(ST36) were selected as the acupoints. The positive and negative electrodes of the electropuncture apparatus(G8650) were respectively connected with Yanglingquan(GB34) and Zusanli(ST36). People reccived transcutaneous electrical acupoint stimulation with condensation-rarefaction wave(2/15 Hz, 8~12mA) untill the end of operation, patiant's body types SBP, SDP, MAP, HR and CSI were recorded at lying still for 5 min(T1), steady state of sevoflurane(T2), acupuncture-assisted anesthesia after 20 and 30 min(T3, T4). [Results] SBP, DBP, MBP, HR and CSI at T3~T4 were significantly lower in group A than those in group B and T2(P<0.05). [Conclusion]Acupuncture-assisted anesthesia can reduce the CSI under the steady-state anesthesia of sevoflurane and lower blood pressure and heart rate at the same time.

Objective:To investigate the effects of TGF-? 1 and TGF-? 2 on cleft palate formation .Methods:30 pregnant NIH mice in experimental group were injected with dexamethasone (50 mg/kg) intr aperitoneally in GD 12 12 ,30 mice in control group were given the same vo lume of NS.Fetuses were removed out in GD13 12 ,GD14 12 and GD15 12 , heads of the mice were sliced in coronal plane.Expression of TGF-? 1 an d TGF-? 2 in embryonic palate were detected by immunohistochemical methods: Results:Dexamethasone (50 mg/kg) induced cleft palate in NI H embryos,and expression of TGF-? 1 and TGF-? 2 in palate tissues was h igher in the experimental group than that in the control (P

Objective To explore drug abuse prevention measures by surveying the changes before and after methadone maintenance treatment(MMT). Methods The baseline survey data were obtained from patients who participated in the MTT program for the first time at Wuhan First Health Clinic of Mental Health Center Affiliated to Tongji Medical College between March 2006 and December 2013,and the general conditions of these patients were analyzed. Results There were 1 186 drug abusers, with a male and low education(junior high school and below)dominance. After the initiation of the MMT program,the number of addicted people was highest in 2008,and then gradually decreased after 2009.MMT program achieved obvious social benefits.The proportion of injectable drug use alone was decreased and the rates of oral drug use and snorting were increased over time.Fixed salary and temporary salary were obviously increased in drug abusers after 2009. Daily drug cost was decreased over time. The proportion of community/ media propaganda through which people got to know MMT remained low. Conclusion The community support and educational propaganda should be strengthened at the same time when MMT is carried out.

Objective To evaluate the efficacy and toxicity of hyperthermia combined with chemoradiotherapy for local advanced cervical cancer. Methods Sixty-eight patients with local advanced cervical cancer were treated. Each patient was given cisplatin at the dose of 20 mg ivgtt dl -2 and 5 -fluorouracil 0.75 ivgtt dl -2, repeating every week, total 3 weeks. Thirty -eight patients received chemoradiotherapy alone. Thirty patients received hyperthermia with deeply focus technology, the temperature was increased and maintained 42.5 ℃ for 30 min at least, within 2 hours following irridiation. Hyperthermia was given twice per week with 7 or 12 sessions, as the same chemoradiation methods as the reference group.After 40 Gy therapy to estimate by cervical examination and ultrasonography, the patients with down stage were for operation, and the others continued to irridiation and additional brachytherapy with californium 252until radical dose. Results All patients were tolerated, fifteen patients of hyperthermia combined group were operated and ten patients of the reference group were operated, the difference was significance (50.0 % vs.26.3 %, P <0.05). The response rate of the combined group was 90.0 %, and the reference group was 84.2 %.The incidence of radiotherapy toxicity of the combined group was not higher than the reference group.Conclusion Hyperthermia combined with chemoradiotherapy can elevate the resection rate and the local control rate, not increase the radiotherapy toxicity and increase quality of life for cervical cancer patients.