Objective To understand the relationship between the changes of coagulation function ,platelet parameters and retic‐ulocyte parameters with the Child‐Pugh grade in the patients with liver cirrhosis .Methods The coagulation function index (PT , APTT ,Fig ,TT) ,platelet parameters(PLT ,MPV ,PDW ,PCT) and reticulocyte parameters(RET# ,RET% ,IRF) were detected in 163 patients with liver cirrhosis and 50 healthy controls by using the automatic hematological analyzer and the automatic blood co‐agulation analyzer .Then the changes of coagulation function ,platelet parameters and reticulocyte parameters were compared be‐tween the liver cirrhotic group and the control groups ,and among different Child‐Pugh grade groups .Results Compared with the control group ,Fig in the liver cirrhosis group was declined ,PT ,APTT and TT were prolonged ,PLT and PCT was decreased ,while MPV and PDW were elevated ,RET count ,RET% and IRF were increased ,the difference between the two groups were statistically significant(P<0 .05);Fig was gradually declined with the Child‐Pugh grade elevation ,PT ,APTT and TT were prolonged ,PLT and PCT was decreased ,while MPV and PDW were increased with the Child‐Pugh grade elevation ,and RET count ,RET% and IRF were increased (P<0 .05) .Conclusion The blood coagulation disturbance and abnormality of platelet parameters and reticulocyte parameters exist in the patients with liver cirrhosis .The changes of coagulation function ,platelet parameters and reticulocyte param‐eters are closely related to the Child‐Pugh classificatione ,which are the important indicators for judging the liver damage ,bleeding tendency and bone marrow hematopoietic function in the patients with liver cirrhosis .

Objective:To explore the correlation between blood pressure and prethrombotic state indexes in patients with chronic heart failure (CHF) .Methods:A total of 96 cases with NYHA classⅡselected from inpatients of our department from Apr 2010 to Feb 2013 were enrolled into CHF group ,another 90 patients with normal cardiac function were regarded as normal control group .Daytime ,nighttime and 24h blood pressure and related indexes of prethrombotic state were detec-ted in both groups .Correlation between blood pressure variability and prethrombotic state indexes were analyzed .Results:Compared with normal control group ,there were significant rise in nighttime diastolic blood pressure [nDBP ,(80.53 ± 7.73) mmHg vs .(84.74 ± 8.74) mmHg] ,daytime systolic blood pressure standard deviation [dSSD ,(11.87 ± 4.98) mm-Hg vs .(14.97 ± 5.12) mmHg] ,night time SSD [nSSD (11.62 ± 4.83) mmHg vs .(15.05 ± 5.42) mmHg] and 24hSSD [24hSSD ,(12.98 ± 3.22) mmHg vs .(15.75 ± 5.08) mmHg] , P<0.01;and significant increase in plasma levels of von Willebrand factor [vWF ,(75.64 ± 6.83) IU/dl vs .(90.75 ± 7.32) IU/dl] ,D-dimer [ (0.31 ± 0.03) g/L vs .(0.73 ± 0.13) g/L] and plasminogen activator inhibitor-1 [PAI-1 ,(22.33 ± 1.14) μg/L vs .(27.46 ± 1.15) μg/L] in CHF group ,P<0.01 all .In CHF patients ,24hSSD were positively correlated with vWF and granule membrane protein (GMP-140) levels (r=0.403 ,0.452 ,P<0.05 both) ,24hDSD and nDSD were positively correlated with GMP-140 level (r=0.421 ,0.413 ,P<0.05 both) ,there were no significant correlation among other blood pressure variability indexes and PTS indexes , P>0. 05 all .Conclusion:In CHF patients ,blood pressure variability and prethrombotic state indexes levels significantly rise ,the both are significant positive correlation .

Objective To investigate the effect of Zhikang capsules on treatment of gastric duodenal ulcer bleeding.Methods Eighty patients with gastric duodenal ulcer bleeding were randomly divided to therapy group(patients received Pantoprazole(20 mg) + Zhikang capsules(1.2 g),2 time/d,n =46) and control group (patients received Pantoprazole,20 mg,2 time/d,n =34).All treatment period was a week.The clinical efficacy were evaluated.Results The rate of total effective of therapy group was 95.7％ (44/46),and control group was 79.4％ (27/34).The difference was statistically significant (x2 =5.164,P ＜ 0.05).Conclusion Zhikang capsules is proved to be an efficacy drug to treatment of gastric duodenal ulcer bleeding,and with less adverse reaction.

Objective To discuss the Clinical significance of blood glucose monitoring in neonate HIE.Methods To monitor glucose concentration in 48 cases of HIE patient and 50 case of normal newborns, and adapt the speed of sugar transport and concentration. Results The neonate HIE was more often have disorder of glucose metabolism than normal newborn, both hyperglycemia and glycopenia could be found in neonate HIE, hyperglycemia could be related with the degree of disease. Conclusion Glucose monitoring in neonate HIE was necessary.

【Objective】To explore the mechanism of Danggui Decoction (DD) in counteracting allergic reaction bymeans of passive cutaneous anaphylaxis (PGA) test.【Methods】Among fifty NEH mice, ten were used to prepareantiserum and the rest forty were randomized into 4 groups: normal control group (A), clarityne group (B), low-doseDD group (C) and high-dose DD group (D) . PGA was induced by intraperitoneal injection of antiserum in the fourgroups and the mice were treated with gastric infusion simultaneously. After the last feeding, antigen attack wasconducted. Inhibitory rate of PCA in four groups were measured by colorimetric analysis after treatment. 【Results】High-and low-dose DD and clarityne could inhibit PCA in mice (P0.05).【Conclusion】The anti-allergic effect of DD may be associated with the inhibition of type Ⅰ allergic reaction.

Objective:To compare the anti-aging effects of ginsenosides,tanshinone and chuanxionyzine on the skin of D-galactose-induced mice aging model.Methods: One hundred female mice were randomly divided into 5 groups(n=20).Mice in normal control group received subcutaneous injection of normal saline and mice in other 4 groups received cervicodorsal injection of D-galactose(1 000 mg?kg~(-1)?d~(-1)) for 42 d to establish subacute aging model.The experimental groups included aging model group(without further treatment),tanshinone-treated(1 500 mg?kg~(-1)?d~(-1)) group,chuanxionyzine-treated(150 mg?kg~(-1)?d~(-1)) group,and ginsenosides-treated(100 mg?kg~(-1)?d~(-1)) group.Forty-two days later,the dorsal skin samples were collected to determine SOD,CAT activities and MDA,hydroxyproline contents.Results: In tanshinone-and ginsenosides-treated groups,SOD activities increased from(131.2?21.5) U/ml to(203.1?11.2) U/ml and(196.8?27.5) U/ml,respectively;the contents of hydroxyprolin increased from(0.57?0.13) mg/g to(0.71?0.11) mg/g and(0.76?0.12) mg/g,respectively;and the contents of MDA decreased from(9.39?1.5) nmol/g to(6.4?1.3) nmol/g and(7.16?2.3) nmol/g,respectively(all P

Objective:To separate and purify polysaccharide MP-Ⅰ from Mytilus coruscus and to investigate its in vitro anti-tumor activity.Methods: The crude polysaccharide was extracted from Mytilus coruscus with hot water and the protein was removed using Sevage method.The polysaccharide MP-Ⅰ was purified using DEAE-Sepharose and Sepharose CL-6B column chromatography.Monosaccharides analysis was carried out using thin-layer chromatography and gas chromatography.The in vitro anti-tumor activity of MP-Ⅰwas assessed using MTT method.Results: The yield of MP-Ⅰwas 2.14%.MP-Ⅰ is mainly constituted by glucose.MP-Ⅰ(at 0.5,0.1,0.02 mg/ml) had different degrees of inhibitory effects on HO-8910,MCF-7, K562,and SMMC-7721 tumor cells in vitro(P

Objective To analyze the results of human papillomavirus(HPV)DNA genotyping detection in 312 female outpatients attending STD clinics.Methods HPV DNA genotyping was detected by fluorescent quantitative polymerase chain reaction(FQ-PCR)for 312 samples collected from female outpatients attending STD clinics.Results The HPV detection rates in outpatients with HPV in their neoplasm and secretion were 83.8% and 38.1%,respectively,and the difference between the two groups had statistical significance.Of the 111 cases with visible neoplasm 4,5 and 57 cases were with 16,18 and 6/11 single type HPV infection,respectively,while 0,16,8 and 3 cases were.with 16+18,16+6/11,18+6/11 and 16+18/11 multiple type infection,respectively.Of the 201 cases with abnormal secretion including cases with other STDs,cases with CA and other STDs in their spouses,treated cases with CA,and cases asking for self-examination because of their risky sex behavior,15,12 and 43 were with 16,18,and 6/11 single type infection,respectively,while 2,5,1 and 0 cases were with 16+18,16+6/11,18+6/11,16+18+6/11 multiple type infection,respectively.Conclusions High HPV DNA rates were detected in neoplasm and secretion of female STD outpatients;although the low risk 6/11 type HPV infection was the main cause,the common high risk type HPV infection was also detected.Therefore,routine HPV DNA detection should be provided to this population group.

Recently, the risk factors for glaucoma is not very clear, a variety of mechanisms, such as increased intraocular pressure,ischemia,deprivation of neurotrophic factors, glial cells activation, glutamate excitatory reactions can promote glaucomatous retinal ganglion cells (RGCs) apoptosis.Through the intervention of nerve cells apoptosis pathway to prevent RGCs damage has become the focus of treatment of glaucoma optic protection.In recent years, studies have shown that interleukin-6 (IL-6) secreted by astrocytes and microglia has the same effect with ciliary neurotrophic factor (CNTF), which can stimulate axon growth and improve the survival rate of RGCs after optic nerve injury and intraocular inflammation.Optic nerve injury and inflammation can induce the expression of IL-6 in retina.IL-6 binds to its receptor expressed in RGCs, activates the Janus kinase (JAK)/signal transducer and activator of transcription (STAT) pathway that up-regulates expression of heat shock proteins,reduces myelin disinhibition of axon regeneration in the optic nerve, resists apoptosis factors, such as tumor necrosis factor-α (TNF-α) and NO, induces regeneration-associated genes expression such as Sprr1a, Gap43, and promotes axon growth.This paper took the relevant researches of IL-6 playing neuroprotective effects on RGCs for a review.

The clinical diagnosis and differential diagnosis of bleeding and thrombotic disorders depend on laboratory detections.Based on clinical diagnosis,the laboratory tests are performed in order and logic according to the principles of optimization and combination of the tests.Then the results were analyzed scientifically to obtain the correct diagnosis.There is important guiding and directing significance for clinic.