1.Regulatory effects of Wuzhuyutang (Evodiae prescription) and its consisting herbs on TPH2 promoter.
Yugang WANG ; Fan LEI ; Xiukun WANG ; Jun HU ; Honglei ZHAN ; Dongming XING ; Lijun DU
China Journal of Chinese Materia Medica 2009;34(17):2261-2264
OBJECTIVETo screen the active component of Wuzhuyutang (WZYT, Evodiae prescription) and investigate the regulatory effects of the components in WZYT on the TPH2 promoter, and to explore the possible molecular mechanism of WZYT on migraine.
METHODBy transfecting a TPH2 promoter regulating Red Fluorescent Protein expressing plasmid into PC12 cell, the global fluorescence intensities and calculations of fluorescent cells after components treatment were statistically evaluated.
RESULTDifferent regulatory effects of different components in WZYT with different concentrations on TPH2 promoter were observed.
CONCLUSIONTPH2 promoter drove Red Fluorescent Protein expressing cell line can be used as system screening components targeting TPH2 promoter activity. The possible mechanism of WZYT on migraine may due to its stimulating effects on TPH2 promoter, and promote the synthesis and release of 5-HT in cerebral.
Animals ; Drugs, Chinese Herbal ; chemistry ; pharmacology ; Evodia ; chemistry ; Humans ; Migraine Disorders ; drug therapy ; enzymology ; genetics ; PC12 Cells ; Promoter Regions, Genetic ; drug effects ; Rats ; Tryptophan Hydroxylase ; genetics ; metabolism
2.Test and discussion on SPECT performance under the Chinese health industry standard WS523-2019
Hui LIU ; Zhan TAN ; Jie YAO ; Hezheng ZHAI ; Zhangfan CHEN ; Ling ZHANG ; Feng WANG ; Honglei LI ; Hongtao HE ; Wangyuan LI ; Shuai ZHANG ; Jianhua GENG
Chinese Journal of Radiological Medicine and Protection 2021;41(7):534-538
Objective:To discuss the problems existing in the implementation of the Chinese health industry standard WS 523-2019 by testing SPECT device.Methods:Under the WS 523-2019 standards, a total of 10 SPECT devices were tested with regard to their SPECT reconstructed spatial resolution (SRSR), system planar sensitivity (SPS), system spatial resolution (SSR), whole body system spatial resolution (WSSR), intrinsic uniformity (IU), intrinsic count rate performance (ICR), intrinsic spatial resolution (ISR) and intrinsic spatial linearity(ISL).Result:Under the requirements of WS 523-2019 standards for qualified limits, there are 3 devices with ISL unqualified and the rest of the performances qualified. The new standards basically can meet the clinical requirements and reflected the overall performance of SPECT.Conclusions:The distance between the radiation source and the surface of the detector has great influence on the spatial resolution.In the measurement of ISL, there must be a lead grid separately in the x and y directions. The lead grids with the parallel slits shall be positioned on the detector with the center slit centered on the detector. It is suggested to add rotation center in the new standards.