In recent years, with the discovery of neural stem cells in the adult nervous system, as well as the continuous research of stem cells, the applications of stem cells in the treatment of ischemic stroke have drawn a broad attention of academicians.A great number of experimental studies have shown that stem cells can improve the post-stroke neurological function to some extent.This indicate a good prospect for clinical application.At present, researches related to the treatment of ischemic stroke with stem cells concentrate mainly on two ways.The first is to treat ischemic stroke through the activating of endogenous neural stem cells, and the second is to treat ischemic stroke through the transplantation of exogenous neural stem cells.This study is designed to review the present research status of the application of various stem cells for the treatment of ischemic stroke in animals and clinical studies, and to analyze existing problems and uncertainties.

OBJECTIVE To exlpore the clinical features and countermeasures of nosocomial infections in cardiovascular patients.METHODS The infection incidence,infection sites,high-risk factors and impacts on prognosis were recorded and analyzed in a retrospective way.RESULTS Nosocomial infection incidence was 10.1% within which respiratory system infection taken the first place;patients over 60 ages and staying in hospital over 30 days,with underlying diseases,chronic heart failure,invasive operation etc were the high-risk factors;the mortality of the infected group was higher than the non-infected group.CONCLUSIONS The infection incidence in cardiovascular patients is related to various factors,with bad prognosis;we need to avoid the high-risk fators based on the active treatment for primary disease,in this way to prevent and control the occurrence of nosocomial infection.

Developed the information centralization and management integration system for monitors of different brands and models with wireless sensor network technologies such as wireless location and wireless communication, based on the existing wireless network. With adaptive implementation and low cost, the system which possesses the advantages of real-time, efficiency and elaboration is able to collect status and data of the monitors, locate the monitors, and provide services with web server, video server and locating server via local network. Using an intranet computer, the clinical and device management staffs can access the status and parameters of monitors. Applications of this system provide convenience and save human resource for clinical departments, as well as promote the efficiency, accuracy and elaboration for the device management. The successful achievement of this system provides solution for integrated and elaborated management of the mobile devices including ventilator and infusion pump.
Computer Communication Networks ; Computers ; Internet ; Wireless Technology

Objective To identify the role of microRNA-17(miR-17)in human glioma U87 cells invasion which may regulate expression of matriv metalloproteinase(MMP)-2.Methods U87 cells were cultured in vitro,while changes in cellular morphology were observed by phase contrast microscope.The miR-17 which might regulate the expression of MMP-2 was predicted by bioinformatics and identified using dual luciferase report system.Expressions of miR-17 and MMP-2 were determined using real-time PCR and Western blot after transfection of miR-17 mimics.The invasion of U87 cells was detected in vitro by Transwell chamber.Results Expression of MMP-2 was positive by immunofluorescence cytochemistry. Using dual luciferase reporter system,miR-17 could inhibit the expression of MMP-2 by binding to its mRNA 3′UTR. Results of real-time PCR and Western blot showed that over-expression of miR-17 down-regulated expression of MMP-2. The invasion of U87 cells was suppressed by over-expression of miR-17.Conclusion MiR-17 may negatively regulate expression of MMP-2 in human glioma U87 cells and inhibit cell invasion.

OBJECTIVE: To evaluate the influence of drinking on hearing loss of pilot students, especially on high frequency hearing loss. METHOD: According to whether drinking, 517 male pilot students were divided into two groups. The balance of all the factors in addition to drinking in both groups was compared. All the students were examined by pure tone audiometry. The statistical data of this research was processed based on a retrospective cohort study. RESULT: The damage of high frequency (4-8 kHz) auditory threshold in left and right ear in drinking group were obviously higher compared with control group (P < 0.01). The Morbidity of hearing loss in drinking group was significantly higher compared to control group (χ2 =16.620, P < 0.01). CONCLUSION Drinking is associated with the hearing loss in pilot students and particularly affects the hearing of high frequency spectrum.
Alcohol Drinking ; adverse effects ; Audiometry, Pure-Tone ; Auditory Threshold ; Cohort Studies ; Deafness ; Ear ; Hearing ; Hearing Loss ; Hearing Loss, High-Frequency ; Hearing Tests ; Humans ; Male ; Retrospective Studies ; Students

Objective To assess the diagnostic value of IMA,NLR,hs-CRP and CK-MB individually and the combined detec-tion for early acute myocardial infarction using ROC curve and Logistic regression.Methods To detect levels of IMA,NLR, hs-CRP,CK-MB and cTnI in serum or whole blood of AMI patients that had chest pain within 3 hours or between 3 and 6 hours,compared with 60 healthy people from Physical Examination Center.Applied Logistic regression,plotted ROC curve and calculated the area under ROC curve (AUC)to assess the diagnostic value of each index.Results The serum IMA,hs-CRP,CK-MB and cTnI or whole blood NLR levels of AMI patients with 3 hours were remarkably higher than normal con-trol,showing significant statistical difference (P<0.01)(AMI group:mean values of IMA,NLR,hs-CRP,CK-MB and cTnI were 96.04 U/L,3.77,13.39 mg/L,43.26 U/L and 0.063 ng/ml;normal control group:mean values of IMA,NLR,hs-CRP,CK-MB and cTnI were 78.10 U/L,2.02,3.12 mg/L,19.37 U/L and 0.040 ng/ml.The serum IMA,NLR,hs-CRP, CK-MB and cTnI levels of AMI patients in the group between 3~6 hours were higher than in the group within 3 hours (P<0.05).The AUC of combined detection of IMA,NLR,hs-CRP and CK-MB for early AMI was 0.98,higher than solo de-tection of IMA,NLR,hs-CRP and CK-MB,which were 0.89,0.83,0.79 and 0.85 respectively.Meanwhile,the AUC of com-bined detection for four markers also surpassed that of cTnI alone that was recognized as a classic serological marker to diag-nose AMI (AUC=0.78).Conclusion The combined detection of IMA,NLR,hs-CRP and CK-MB is superior to a single in-dex detection,which can significantly improve diagnostic efficiency for early AMI.

Objective To characterize the language development and articulation in children with different types of cerebral palsy. Methods The data from 76 children with cerebral palsy who underwent standardized tests of speech and language were analyzed. The incidence and abnormal pattern were compared between groups. ResultsLanguage delay and/or dysarthria were noted in 73.1% of these subjects. Children with diplegia developed similar levels both in comprehension and verbal expression, whereas those with tetraplegia and athetoid developed poorer verbal expression. The incidence of language delay in children with diplegia, tetraplegia and athetoid were 45.95%, 90% and 64.7%, respectively. Dysarthria was found in all the children with tetraplegia, athetoid and ataxia, but only 48.65% of those with diplegia. Conclusion Language disorders were common in children with cerebral palsy, especially those with athetoid and tetraplegia.

Objective To determine the clinical indications and detection efficiency of non-invasive prenatal testing (NIPT) in Jiangsu Province, China. Methods A total of 13 041 pregnant women from nine hospitals in Jiangsu Province who voluntarily accepted NIPT for chromosome 13, 18, 21 and sex chromosome from January 1, 2012 to December 31, 2013 were analyzed retrospectively. All cases were singleton pregnancies and spontaneously conceived. Invasive prenatal diagnosis followed by fetal chromosome karyotype analysis was recommended in high-risk women following NIPT. The clinical indications and positive predictive value of NIPT were conducted. Results NIPT detected 88, 19, 9 and 64 cases at high risk for trisomy 21, trisomy 18, trisomy 13 and X chromosome aneuploidy, and the positive rate was 0.67%, 0.15%, 0.07% and 0.49%, respectively. Among the 74, 13, 8 and 44 high-risk cases who accepted chromosome karyotype analysis, respectively, 67 cases were diagnosed with trisomy 21, 12 cases with trisomy 18, one case with trisomy 13, and 18 cases with numerical X chromosome abnormality. The positive predictive value was 90.5% (67/74), 12/13, 1/8 and 40.9% (18/44), respectively. One pregnant woman who was reported as high-risk trisomy 21 following NIPT, but high-risk trisomy 18 at prior serum screening, was eventually diagnosed with fetal trisomy 18 by chromosome karyotype analysis, whose placenta was a mosaic of trisomy 21 and trisomy 18. High-risk following serum screening was the most common indication for NIPT accounting for 46.4% (6 056/13 041), followed by low-risk but asking for testing (28.9%, 3 773/13 041) and advanced age (20.5%, 2 673/13 041). Conclusions High-risk, low-risk but asking for testing and advanced maternal age are common indications for NIPT in Jiangsu Province. The positive predictive value of NIPT for trisomy 21 or trisomy 18 is relatively high, but is much lower for trisomy 13 or X chromosome aneuploidy.

Objective To investigate the effects of different types of anesthesia on the incidence rates of low-er respiratory tract infections of elderly patients in orthopedic department.Methods Clinical data from 445 elderly patients in orthopedic department were collected and retrospectively analyzed.We also investigated the relationship be-tween the types of anesthesia and the lower respiratory tract infections.Results The incidence rates of lower respira-tory tract infections were 23.08%in 169 cases,12.14%in 173 cases,and 4.85%in 103 cases after general anesthe-sia with tracheal intubation,intraspinal anesthesia,and nerve blocking anesthesia,respectively.There was statistically significant difference in the infection rates among the three groups (χ2 =13.784,P=0.001).Conclusion Different ways of anesthesia is critically correlated with the incidence of the lower respiratory tract infections in elderly patients.

Objective To investigate the effectiveness of target intervention in preventing catheter -related bloodstream infection ( CRBSI ) .Methods The targeted monitoring was launched by changing venepuncture site , reinforcing instructions in aseptic insertion technique and hand hygiene supervision ,avoiding femoral access and remo-ving unnecessary catheters ,venous puncture implemented by high qualification anesthesiologist ,then the incidence of CRBSI before and after the project was compared .Results The incidence of CRBSI after the project decreased signif-icantly from 17.97%to 4.32%,the difference was statistically significant (χ2 =40.82,P<0.05).Chi-square test and logistic regression analysis showed that the incidence of infection was increased in patients with catheter for longer than 10 days (χ2 =4.33,P<0.05),the incidence of CRBSI was decreased in patients that central venous puncture implemented by high qualification anesthesiologist (χ2 =9.21,P<0.05),and patients received catheter indwelling into femoral vein had increased incidence of CRBS (χ2 =7.45,P<0.05).Conclusion Indwelling catheter time, unskilled operator ,catheter site are risk factors in patients with CRBSI .In response to these factors ,we should develop interventions to maximize establish a protective barrier , improve the quality of the medical operation , prevent the occurrence of CRBSI more efficiently .