Objective To investigate the expression of CKLF?2 mRNA in rats′ myocardium at different development phase. Methods Total RNA was extracted from fetal rat hearts at day12 and day 18 after coitus, and from new-born rat hearts right after birth, day 3, day 7 and day 21 after birth, as well as from adult rat hearts. The expression of CKLF?2 mRNA was tested by competitive polymerase chain reaction (CPCR). Results Compared with the postnatal myocardium, the expression of CKLF?2 mRNA obtained its peak level in the 12-day-post coitus, which then decreased gradually to a relatively low level until birth. It increased slightly at birth and subsided to the lowest level in adulthood. Conclusion CKLF?2 probably takes part in the procession of the myocardial proliferation and the development of rat′s heart.

ThisstudywasaimedtoobservetheinhibitoryeffectsofQi-Zhu (QZ)granulesonearlyproteinuria in diabetic nephropathy rats with syndrome of qi-yin deficiency and phlegm blocking collaterals. A total of 44 rats were randomly divided into the blank group, model group, Huang-Kui capsule group, QZ granules group. The rat model of diabetic nephropathy with syndrome of qi-yin deficiency and phlegm blocking collaterals was induced by the combination of unilateral renal artery ligation, diet of high-calorie and high cholesterol, and intraperitoneal injection of low-dose streptozotocin. The medication was given for 8 weeks. The concentrations of protein and creatinine in urine were observed on the 4th week. The blood glucose, blood lipids, liver function and renal pathological changes were observed at the end of the experiment. The results showed that compared with the model group, QZ granules can obvious suppress early proteinuria in diabetic nephropathy, promote creatinine excretion, regulate blood lipid metabolism, protect liver function and improve renal pathological changes. It was concluded that QZ granules had independent inhibition effect on early proteinuria in diabetic nephropathy rats with syndrome of qi-yin deficiency and phlegm blocking collaterals. The effect was independent of lowing blood glucose. It represented the corresponding relation between the syndrome and efficacy in Chinese herb compounds.