Objective To explore the effect of microRNA miR?143 on the proliferation of cervical cancer HeLa cells through targeted regulating the expression of K?ras gene. Methods The luciferase report carrier containing wild type 3′?UTR of K?ras gene ( K?ras?wt) or mutated 3′?UTR of the K?ras ( K?ras?mut) were co?transfected with iR?143 mimic into the HeLa cells respectively, and the targeting effect of miR?143 in the transfectants was verified by the dual luciferase report system. HeLa cells were also transfected with miR?143 mimic ( miR?143 mimic group) , mimic control ( negative control group) , and miR?143 mimic plus K?ras gene ( miR?143 mimic+K?ras group) , respectively. The expression of miR?143 in the transfected HeLa cells was detected by real?time PCR ( RT?PCR ) , and the expression of K?ras protein was detected by Western blot. The cell proliferation activity of each group was examined by MTT assay. In addition, human cervical cancer tissue samples ( n=5) and cervical intraepithelial neoplasia tissue samples ( n=5) were also examined for the expression of miR?143 and K?ras protein by RT?PCR and Western blot, respectively. Results The luciferase report assay showed that co?transfection with miR?143 mimic decreased the luciferase activity of the K?ras?wt significantly, but did not inhibit the luciferase activity of the K?ras?mut. The expression of miR?143 in the HeLa cells transfected with miR?143 mimic was significantly higher than that in the HeLa cells transfected with the mimic control (3.31±0.45 vs 0.97±0.22, P<0.05). The MTT assay revealed that the cell proliferative activity of the miR?143 mimic group was significantly lower than that of the negative control group (P<0.05), and the cell proliferative activity of the miR?143 mimic+K?ras group was also significantly lower than the control group ( P<0.05) but higher than the miR?143 mimic group significantly (P<0.05). The expression levels of K?ras protein in the miR?143 mimic group, the negative control group and the miR?143 mimic+K?ras group were lowest, moderate, and highest, respectively (115.27±34.08, 521.36±41.89, and 706.52±89.44, all P<0.05). In the tissue samples, the miR?143 expression in the cervical cancer group was significantly lower than that of the cervical intraepithelial neoplasia group (0.32±0.06 vs. 0.93±0.17, P<0.05);whereas the K?ras protein expression in the cervical cancer group was significantly higher than that in the cervical intraepithelial neoplasia group ( 584. 39 ± 72.34 vs. 114.23±25.82, P<0.05). Conclusions In vitro, miR?143 can inhibit the proliferative activity of HeLa cells through targeted regulating the expression of K?ras gene. In human cervical cancer tissues of a small sample set, the expression of miR?143 is downregulated, and the expression of K?ras is upregulated.

Objective To explore the effect of microRNA miR?143 on the proliferation of cervical cancer HeLa cells through targeted regulating the expression of K?ras gene. Methods The luciferase report carrier containing wild type 3′?UTR of K?ras gene ( K?ras?wt) or mutated 3′?UTR of the K?ras ( K?ras?mut) were co?transfected with iR?143 mimic into the HeLa cells respectively, and the targeting effect of miR?143 in the transfectants was verified by the dual luciferase report system. HeLa cells were also transfected with miR?143 mimic ( miR?143 mimic group) , mimic control ( negative control group) , and miR?143 mimic plus K?ras gene ( miR?143 mimic+K?ras group) , respectively. The expression of miR?143 in the transfected HeLa cells was detected by real?time PCR ( RT?PCR ) , and the expression of K?ras protein was detected by Western blot. The cell proliferation activity of each group was examined by MTT assay. In addition, human cervical cancer tissue samples ( n=5) and cervical intraepithelial neoplasia tissue samples ( n=5) were also examined for the expression of miR?143 and K?ras protein by RT?PCR and Western blot, respectively. Results The luciferase report assay showed that co?transfection with miR?143 mimic decreased the luciferase activity of the K?ras?wt significantly, but did not inhibit the luciferase activity of the K?ras?mut. The expression of miR?143 in the HeLa cells transfected with miR?143 mimic was significantly higher than that in the HeLa cells transfected with the mimic control (3.31±0.45 vs 0.97±0.22, P<0.05). The MTT assay revealed that the cell proliferative activity of the miR?143 mimic group was significantly lower than that of the negative control group (P<0.05), and the cell proliferative activity of the miR?143 mimic+K?ras group was also significantly lower than the control group ( P<0.05) but higher than the miR?143 mimic group significantly (P<0.05). The expression levels of K?ras protein in the miR?143 mimic group, the negative control group and the miR?143 mimic+K?ras group were lowest, moderate, and highest, respectively (115.27±34.08, 521.36±41.89, and 706.52±89.44, all P<0.05). In the tissue samples, the miR?143 expression in the cervical cancer group was significantly lower than that of the cervical intraepithelial neoplasia group (0.32±0.06 vs. 0.93±0.17, P<0.05);whereas the K?ras protein expression in the cervical cancer group was significantly higher than that in the cervical intraepithelial neoplasia group ( 584. 39 ± 72.34 vs. 114.23±25.82, P<0.05). Conclusions In vitro, miR?143 can inhibit the proliferative activity of HeLa cells through targeted regulating the expression of K?ras gene. In human cervical cancer tissues of a small sample set, the expression of miR?143 is downregulated, and the expression of K?ras is upregulated.

Objective:To investigate the intervention effect of decision aid on the fear of falling in elderly patients after total hip arthroplasty.Methods:This study was a quasi experimental research. From June 2021 to November 2021, 84 patients after total hip arthroplasty who were admitted to the Department of Orthopedics of Zhengzhou Central Hospital Affiliated to Zhengzhou University were selected as the research objects. According to the order of admission, they were divided into the control group (42 cases, 2 cases fell off ) and the observation group (42 cases). The control group was given routine nursing care and guidance for fear of falling, while the observation group was given a decision-making aid intervention program on the basis of the control group. The time to first getting out of bed, the scores of Modified Falls Efficacy Scale (MFES), Generalized Anxiety Disorder (GAD-7) and Harris Hip Score (HHS) were compared between two groups.Results:The time to get out of bed for the first time in the observation group was (42.71 ± 6.41) h, lower than that in the control group (49.95 ± 5.73) h, and the difference was statistically significant ( t=5.38, P<0.05). Twelve weeks after discharge, the GAD-7 score in the observation group was (4.64 ± 1.43), which was lower than that of the control group (6.85 ± 1.83), and the difference was statistically significant ( t=6.10, P<0.05). The score of the MFES in the observation group was [8.50(8.00,10.00)], which was higher than that in the control group [7.50(7.00,8.00)], and the difference was statistically significant ( Z=-6.26, P<0.05). The hip joint function score of the observation group was (81.74 ± 4.24), which was higher than that of the control group (74.30 ± 4.51), and the difference was statistically significant ( t=7.69, P<0.05). Conclusions:Decision support can advance the time of downward movement of elderly patients after total hip arthroplasty, reduce their anxiety and fear of falling, and improve hip function.

Objective To compare the safety and efficacy of direct thrombectomy versus bridging thrombectomy in the treatment of acute anterior circulation large vessel occlusion stroke under different collateral circulation statuses.Methods Totally 93 patients with acute anterior circulation ischemic stroke admitted to the First Affiliated Hospital of Xinxiang Medical University from September 2020 to March 2023 were selected as the research subjects.Patients were divided into direct throm-bectomy group(n=47)and bridging thrombectomy group(n=46)based on the type of thrombectomy.Patients in the direct thrombectomy group received direct intravascular thrombectomy,while patients in the bridging thrombectomy group received intravenous thrombolysis with alteplase combined with mechanical thrombectomy.According computed tomography angiography,the collateral circulation Tan classification was applied to divide the patients into good collateral circulation sub-group and poor collateral circulation sub-group.The modified thrombolysis in cerebral infarction grading(mTICI)was used to evaluate vessel recanalization.Head computed tomography plain scan was performed at 24-48 hours postoperatively to assess if there was hemorrhagic transformation,and modified Rankin Scale score was performed at 90 days postoperatively.Information such as imaging examination time,femoral artery puncture time,vessel recanalization time after thrombectomy,prognosis and spontaneous non-traumatic symptomatic intracerebral hemorrhage(SICH)were collected.Results The age,gender,baseline Alberta stroke program early computed tomography score,baseline national institutes of health stroke scale score,proportions of hypertension,diabetes and atrial fibrillation,baseline systolic pressure,creatinine,baseline blood glucose,platelet count,occlusion site,stroke etiologies and collateral circulation status of patients in the two groups were not statistically significantly different(P＞0.05).There were no significant differences in the post-admission imaging examination time,femoral artery puncture time,vessel recanalization time after thrombectomy,successful vascular reperfusion rate,good prognosis rate,mortality rate,and SICH incidence between the two groups(P＞0.05).The hemorrhagic transformation rate of patients in the direct thrombectomy group was significantly lower than that in the bridging thrombectomy group(P＜0.05).There were no significant differences in the post-admission imaging examination time,femoral artery puncture time,vessel recanalization time after thrombectomy,successful vascular reperfusion rate,good prognosis rate,mortality rate,and SICH incidence between patients with good collateral circulation and patients with poor collateral circulation in the two groups(P＞0.05).The hemorrhagic transformation rate of patients with good and poor collateral circulation in the direct thrombectomy group was significantly lower than that in the bridging thrombectomy group(P＜0.05).Conclusion Under different collateral circulation conditions,the safety and efficacy of direct thrombectomy and bridging thrombectomy in the treatment of acute anterior circulation large vessel occlusion stroke are similar,but bridging thrombectomy is more likely to result in cerebral hemorrhage transformation compared with direct thrombectomy.

Objective To explore the relationship between collateral circulation and the prognosis of acute ischemic stroke(AIS)patients with successful recanalization after mechanical thrombectomy.Methods A total of 75 AIS patients who received mechanical thrombectomy at the First Affiliated Hospital of Xinxiang Medical University from September 2020 to May 2022 were selected as the research subjects.The clinical data of the patients were collected.According to the modified Rankin scale(mRS)score at 90 days after operation,the patients were divided into the good prognosis group(mRS score≤2)and the poor prognosis group(mRS score ≥3).Univariate and multivariate logistic regression were used to analyze the factors affecting the prognosis of AIS patients with successful recanalization after mechanical thrombectomy.There were three types of collateral circulation:symmetrical collateral circulation,malignant collateral circulation,and other collateral circulation.The National Institutes of Health Stroke Scale(NIHSS)score at admission,the NIHSS score at discharge and changes of the NIHSS score of patients with different collateral circulations were compared.Results Among the 75 patients,43 had a good prognosis,and 32 had a poor prognosis.The incidence of poor prognosis was 42.67％.Univariate analysis showed that history of diabetes,time from onset to puncture(OPT),collateral circulation and NIHSS score were the factors effecting the prognosis of AIS patients with successful recanalization after mechanical thrombectomy(P＜0.05).Age,sex,history of atrial fibrillation,history of hypertension,history of smoking,history of stroke,thrombolytic therapy and modified thrombolysis in cerebral infarction grade were not related to the prognosis of AIS patients with successful recanalization after mechanical thrombectomy(P＞0.05).Multivariate logistic regression analysis showed that symmetrical collateral circulation,malignant collateral circulation,history of diabetes and OPT were independent influencing factors for the prognosis of AIS patients with successful recanalization after mechanical thrombectomy(P＜0.05).There was no significant difference in the admission NIHSS score among the patients with different collateral circulations(P＞0.05).The discharge NIHSS score of patients with symmetrical collateral circulation was significantly lower than that of patients with malignant collateral circulation,and the change in the NIHSS score was more significant than that of patients with malignant collateral circulation(P＜0.05).There was no significant difference in the discharge NIHSS score and change in the NIHSS score between patients with symmetrical collateral circulation and patients with other collateral circulation(P＞0.05),and between patients with malignant collateral circulation and patients with other collateral circulation(P＞0.05).Conclusion Symmetrical collateral circulation,malignant collateral circulation are independent factor affecting the prognosis of AIS patients with successful recanalization after mechanical thrombectomy.Symmetrical collateral circulation may help improve the neurological function of patients after mechanical thrombectomy,and malignant collateral circulation may lead to deterioration of neurological function in patients undergoing mechanical thrombectomy.