Heat shock protein (HSP) 105 is a 105x103 stress protein that belongs to the HSP-110 family. It is released by tissues in response to a wide variety of stresses including infection and ischaemia. Studies have shown that the molecule is involved as a biochemical mediator of heat induced apoptosis by binding to p53 at scrotal temperatures and dissociating from it at suprascrotal temperatures in testicular germ cells. Recent studies have shown that HSP-105 is over-expressed in various malignancies besides in normal testicular tissue. HSP-105 may be a candidate of testis antigen.

Objective To evaluate clinical effect of mild hypothermia on patients with severe brain injury.Methods 40 patients with severe brain injury were randomly divided into hypothermic group(n=20) and control group(n=20).Their excellence rates cvere compared.Results The total excellence rate of treatment group was 80% and that of control group was 50%,the former was significantly higher than the latter.Conclusion Mild hypothermia can improve the outcome of patients with severe brain injury.

Aim To construct a screen assay of ?-secretase inhibitors.Methods A human ?-secretase cDNA was cloned by RT-PCR and then reconstructed in an expression vector.The recombinant ?-secretase fusion protein was expressed and purified from Escherichia coli.A screen assay of ?-secretase inhibitors was constructed in vitro using a recombinant A ?-fusion protein as substrate.The enzyme reaction was detected by SDS-PAGE and an ELISA method using metal chelating plates to catch the substrate.Results A human ?-secretaae cDNA was cloned and expressed in escherichia coli.The ?-secretase enzyme reaction can be detected by two kinds of method: SDS-PAGE and ELISA.The IC_(50) for an inhibitor statVal determined by this method was in good agreement with those reported in the literature.Seventy seven species of Chinese traditional medical plants were screened using this method and seventeen species show weak inhibitory activity.Conclusion A screen assay of ?-secretase inhibitors was successfully constructed and can be used to screen natural products.

For engineering an efficient butanol-producing Escherichia coli strain, many efforts have been paid on the known genes or pathways based on current knowledge. However, many genes in the genome could also contribute to butanol production in an unexpected way. In this work, we used Tn5 transposon to construct a mutant library including 1 196 strains in a previously engineered butanol-producing E. coli strain. To screen the strains with improved titer of butanol production, we developed a high-throughput method for pyruvate detection based on dinitrophenylhydrazine reaction using 96-well microplate reader, because pyruvate is the precursor of butanol and its concentration is inversely correlated with butanol in the fermentation broth. Using this method, we successfully screened three mutants with increased butanol titer. The insertion sites of Tn5 transposon was in the ORFs of pykA, tdk, and cadC by inverse PCR and sequencing. These found genes would be efficient targets for further strain improvement. And the genome scanning strategy described here will be helpful for other microbial cell factory construction.
Butanols ; chemistry ; DNA Transposable Elements ; Escherichia coli ; metabolism ; Fermentation ; Gene Library ; Hydrazines ; Industrial Microbiology ; Mutagenesis ; Open Reading Frames ; Organisms, Genetically Modified ; Polymerase Chain Reaction ; Pyruvic Acid ; chemistry

Objective To assess the diagnostic value of CT and MRI findings of toxoplasma encephalitis among AIDS patients.Methods CT and MRI findings of toxoplasma encephalitis were retrospectively studied in 17 AIDS cases.Results The lesions were bilateral and multiple,involving basal nuclei in 12 cases,thalamus in 3 cases,bilateral cerebral hemispheres near corticomedullary junction in 1 case,and cerebellum and brain stem in 1 case.The lesions showed weak hypodensity on CT,and long T 1 and long T2 signal intensity on MRI,with marked peripheral edema effect.After contrast administration,the small ring or twist and target enhancement was seen in 12 cases,large ring enhancement-in 1 case.multiple focus in 15 cases and single focus in 2 cases;MRI was more sensitive in detecting a largest number of cerebral lesions than CT.Conclusion More small ring and twist,nodular,target enhancement are highly suggestive of toxoplasma encephalitis in the basal nuclei.Both CT and MRI are effective in diagnosing toxoplasma encephalitis,but MRI imaging without and with gadolinium is more sensitive than CT in the detection of toxoplasma encephalitis;MRI imaging may reveal a greater number of lesion when it is positive and so the detection rate of MRI is higher than that of CT.

Mass spectrometry-based quantitative proteomics has become an indispensable tool for tumor marker discovery.Stable isotope labeling with amino acid in cell culture(SILAC),as a representative of in vivo metabolic labeling strategy,is a simple and relatively quantitative assay for comparative proteomics.Two kinds of cultured cells are grown in the medium containing either a light or heavy isotope labeled essential amino acids.After several cell doublings,the heavy amino acids are introduced into the nascent polypeptides in a sequence-specific fashion and the natural amino acid is completely replaced by its isotopically labeled analog.Accuracy quantification would be feasible by analyzing the peak intensities of every same peptide pairs labeled with light or heavy isotopes by the mass spectrometry.Compared with chemical labeling,SILAC requires much less amount of labeled proteins,and it is an efficient,straightforward,reproducible and accurate approach in cell-based systems.This method can not only investigate the dynamics of protein abundance,but also monitor the variation of posttranslational modifications and protein-protein interactions qualitatively and quantitatively.It is a powerful and important quantitative proteomics tool for tumor marker discovery.

[Objective]To find out the new method to cure the intervertebral discs degeneration by way of the eletron microscope study of the degeneration of the cartilage endplate in the circumstances of the intervertebral destabilization. [Method]To choose forty-eight white macrotia rabbits of Japan,which weighed within the range of 2.5?0.2 kg,female and male was unrestricted.These rabbits were divided into 2 groups randomly,with 21 rabbits in the contrast troup,with 27 rabbits in the experimental group.First 27 rabbits of the experimental group were destabilizated by operated on L6、7.Inj Diazepam 0.25 mg/kg,Inj Ketamine 0.02 g/kg and Inj Atropine 0.125 mg/kg was one by one injected into the rabbit through the auris vein,shearing the rabbit hair of backside waist,fixing the rabbit on operation table in face lying,using 1% Povidone Ioding to degerm the operation area.Every rabbit was incised at the backside of its waist,that incisal opening is located in the center of the intervertebral space(L6、7 space) that both side iliac crest line correspond,from the meso-ordinate direction cut about 4 cm incision,cutting open skin and subcutaneous tissue,thoroughly,exposing the spinous processes the vertebral plates and the upper-inferior articular processes,entirely segregating the muscles that cohere the spinous processes the vertebral plates and the articluar process,then excising the supraspinal ligaments and interspinal ligaments,biting off two sides the inferior articular processes of L6,in order that resulting in intervertebral destabilization,using 0.9% Inj.Sodium Chloride to washout the incisal opening,in order sewing up each layer tissues.After operation rabbits can freely move in the cages.To draw the materials from all groups after two months,four months and six months orderly.Using transmission electron microscope to observe the structure the cartilage endplate,the author should judge the degenerative course and mechanism of the cartilage endplate synthetically.[Result]The intervertebral destabilization can cause the collagen fibers degenerates from the regular and compact structure to the disorder and loose structure.[Conclusion]The intervertebral destabilization can distinctly lead to the degeneration of the cartilage endplate.

Objective:To study the effects of hydroxyapatite(HA) coating on sandblasted and acid-etched(SLA) surface of titanium dental implants(HA-SLA-Ti) on the biologic characteristics of marrow-origin osteoblasts(MOOBs).Methods:Coating of HA on the SLA surface of titanium dental implants were formed by ion beam assisted deposition (IBAD) method. MOOBs,cultured in vitro,were seeded onto the surface of HA-SLA-Ti and SLA-Ti respectively, the growth of MOOBs on the two kinds of samples were observed by scaning electron microscope.The proliferation index, alkaline phosphatase(AKP) activity, osteocalcin(BGP) content of MOOBs and mRNA relative expression level of osteopontin(opn) were examined and compared between two groups.Results: MOOBs grew well on the surface of HA-SLA-Ti; the proliferation index, AKP activity and BGP content of MOOBs were higher in HA-SLA-Ti group than those in SLA-Ti group(P

0.05). Conclusion:Ti-29Nb-13Ta-4.6Zr alloy is histocompatible.