Objective To study the effect of estrogen on cardiac injury and cardiomyocyte apoptosis of rat induced by isoprotere-nol by modeling cardiac inj ury induced by bilateral ovariectomized (OVX)and isoproterenol (ISO).Methods Fifty female SD rats with bilateral ovariectomy and sham operation (Sham)were randomly divided into 5 groups:sham operation group (Sham group), bilateral ovariectomy group (OVX group),cardiac injury group (OVX+ISO+Vehi group),low dose estrogen treatment group (OVX+ISO+E2 a group,4μg·kg-1 ·d-1 ),high dose estrogen treatment group (OVX+ISO+E2 b group,40μg·kg-1 ·d-1 ). these status were separately measured:rats′general features,hemodynamics parameters monitored of carotid artery,morphological observation and cardiomyocyte contraction change of single-cardiomyocyte separate cultured,cardiomyocyte apoptosis protein ex-pression were detected by immunoblotting.Results ISO significantly reduced myocardial pump function,increased hypertrophy and apoptosis of cardiomyocytes,reduced contractility of single cardiomyocytes (P<0.05).High-dose estrogen (40μg·kg-1 ·d-1 ) replacement therapy significantly improved ISO induced cardio inj ury and cardio functions decreasing,also inhibited Bax expression and caspas-3 activation and decreased myocardial hypertrophy and cardiomyocytes apoptosis through increasing Bcl-2 expression (P<0.05),significantly.while low dose estrogen (4μg·kg-1 ·d-1 )treatment showed marginally protection effects on ISO in-duced cardio inj ury with no statisticly significance.Conclusion Appropriate dose estrogen replacement therapy can decrease cardio-myocyte apoptosis,improve cardiomyocytes contractility,so as to protect ISO-induced cardiomyocyte apoptosis.

Objective: To investigate the rate of depression among Beijing residents aged 55 or above. Methods: 2660 Beijing residents aged 55 or above in a cohort study of year 2000 were followed and screened with CES-D. Demographic data, information of health status and activity of daily living were also collected. Results: The rate of depression in this cohort was 13.5% (360/2660), the rate of men was 9.6%, that of women was 17.4%, women had significantly higher rate than men. The rate of depression of residents in rural area was much higher than that of urban area (20.9% vs. 7.4%), the illiterate resident had higher rate of depression than those with at least primary education (20% vs. 8.7%). Residents above 75 or below that had no difference in rate of depression. Depression had close relation with poor health status and disability in activity of daily living. Conclusion:The rate of depression is higher in women and in residents of rural area or in those illiterate. Depression is closely related to poor health status and disability in daily living.

ObjectiveTo investigate the value of serum creatinine(SCr) to monitor the renal function of patients with spinal cord injury(SCI).Methods1298 SCI patients were investigated in Beijing Charity Hospital from January 2004 to May 2009. 249 healthy people were involved as control. The data of SCr were analysed.ResultsThe level of SCr for the SCI patients was (56.81±14.33) μmol/L with normal distribution, 95% CI was 28.73～84.89 μmol/L; The level of SCr for the healthy people was (75.98±11.34) μmol/L, 95% CI was 57.36～101.80 μmol/L. The level of SCr was significantly lower in the SCI patients comparing to the healthy people (P<0.01). Among the SCI patients, the level of SCr was (59.59±13.76) μmol/L for male while (46.41±11.31) μmol/L for female(P<0.05). There was no difference in level of SCr among different ages of the SCI patients(P>0.05). The characteristics were likely in the two groups. There was no difference in level of SCr between paraplegic (55.54±14.96) μmol/L and quadriplegic (57.67±13.83) μmol/L (P>0.05).ConclusionThe characteristics of level of SCr were likely in SCI patients and healthy people with normal distribution. The level of SCr was lower in SCI patients comparing to healthy people, which was higher for male SCI patients comparing to female SCI patients.

Objective To investigate the serum lipids levels of Chinese men with chronic paraplegia.Methods A total of 650 male paraplegics admitted to the China Rehabilitation Research Center (CRRC) for rehabilitation therapy from Jan 2004-Dec 2008 were investigated. The inclusion criteria were a definite paraplegic history (T2-L5 spinal cord injury); complete data; age 18-60 years; and no history of stroke. TG, TC, LDL and HDL serum lipid profiles were studied. Results These male paraplegics showed significantly higher mean TG and TC levels and lower mean HDL levels than the nation-wide averages for similar males ( TG 1.72 mmol/L vs. 1.36 mmol/L; TC4.42 mmol/L vs. 4.30 mmoL/L; HDL 0.99 mmol/L vs. 1.18 mmol/L). No difference in average LDL levels was found. TG, TC and LDL levels were correlated positively with age, but HDL was not. Duration of paraplegia correlated positively with HDL levels, but negatively with LDL. The prevalence of dyslipidemia was 67% , and the prevalence of low HDL was 64%. Conclusions The serum lipid profiles of the young male paraplegics showed a high prevalence of dislipidemia, significantly low HDL levels with a high prevalence of low HDL, and no correlation with age. TG, TC and LDL levels increased with age. The duration of paraplegia was correlated positively with HDL but negatively with LDL, probably due to increased exercise of the upper limbs.

Objective To investigate the protective effect of umbilical cord mesenchymal stem cells (UCMSCs) on traumatic brain injury (TBI) in rats.Methods Thirty healthy Sprague-Dawley rats (10 rats for each group) were randomly divided into normal control group (normal),model group (injection of saline after TBI) and UCMSCs transplantation group (injection of UCMSCs after TBI).The rats in experimental groups were sacrificed on the 10th day after UCMSCs transplantation.The percentage of UCMSCs in brain tissue was detected by flow cytometry.The pathological changes of brain tissue were observed by hematoxylin-eosin (HE) staining method.The expressions of vascular endothelial growth factor (VEGF),glial fibrillary acidic protein (GFAP) and brain-derived neurotrophic factor (BDNF) in brain tissue were measured by immunohistochemistry and immunofluorescence double staining.The neurological deficit was evaluated by neurological deficit degree.Results The percentage of CD90,CD73 and CD105 cells in the UCMSCs transplantation group was significandtly higher than that in the model group (0.4％ vs 0.1％,P＜0.05).The results of HE staining showed that the brain injury of the transplanted group was alleviated compared with the model group (P＜0.05).The VEGF of the brain tissue in injury area in the UCMSCs transplantation group was higher than that in the model group (P＜0.05).The number of GFAP and BDNF positive cells in the UCMSCs transplantation group was higher than that in the model group (P＜0.05),and the neurological deficit score was also higher than that in the model group (P＜0.05).Conclusions UCMSCs transplantation for the treatment of TBI rats can effectively reduce the vascular damage in the injury area and promote nerve recovery.

ObjectiveTo explore the arrhythmia happened in patients with cervical spinal cord injury (CSCI). Methods61 patients with CSCI were retrospectively analyzed. ResultsThe overall incidence of sinus bradycardia was 26.2%, which was higher in the cases within 3 months (52.4%) than over (12.5%); and was higher in cases injured the cord above C5（46.2%）than lower (C6-8,11.4%). ConclusionThe risk of arrhythmia is still high in chronic phase, which still need to monitor.

Objective To summarize the clinical nursing experience of a human cytomegalovirus infection combined with infantile polymorphic erythema.Methods Through evidence-based nursing,the family members of the children and their families were evaluated in two ways and carried out the familycentered holistic nursing care.The nursing problems of the children should be evaluated in a timely and effective manner.Results After 15 days of treatment,the patient was discharged from hospital and achieved satisfactory results.Conclusions For human cytomegalovirus infection with baby severity exudative polymorphic lupus patients,the implementation of strict protective isolation,meticulous care of skin mucous membrane,prevent eye and perineal secondary infection,close observation and prevention of severe complications in children with play a crucial role in the course of the disease and rehabilitation.

Objective To evaluate the role of laparoscopic ultrasonography (LUS) in difficult place laparoscopic hepatectomy of the right liver.Methods The data of 7 patients undergoing laparoscopic hepatectomy with tumor in the difficult location of the right liver in our hospital between Jun 2015 and Aug 2017 were retrospectively analyzed.LUS was used during all the operations in order to investigate the anatomy of the operations,determine the tumor stage and guide the incision margin of tumor.It was conventionally used to detect the relationship between the lesions and peripheral intrahepatic vessels and ducts.Results Seven cases underwent successful laparoscopic hepatectomy.The mean operative time was (121 ± 50) min,average intraoperative blood loss was (301 ± 122) ml,and there was no operative mortality.Mean hospital stay was (7.1 ± 1.2) d.One suffered biliary leakage and was cured by conservative treatment.Conclusions Laparoscopic ultrasound can determine tumor location and guide the operative route,increasing safety of laparoscopic hepatectomy.

Objective To apply Canadian Occupational Performance Measure (COPM) on the patients injured in Sichuan Earthquake. Methods COPM was applied to evaluate 51 patients in hospital before and 1 months after treatment. The first evaluation confirmed the problems on occupational activities for them. Then the plan was made to solve the problems. The second evaluation was to assess the effect of the treatment.Results The problem of self-care activity is more than that of productive activities and leisure activities(P<0.01). Total scores of performance of occupational activity and satisfaction improved(P<0.01, P<0.01)Conclusion COPM is helpful to confirm the problems of occupational activities and contribute to develope the primary goals for rehabilitation and treatment programs.

OBJECTIVETo study the relationship between body mass index (BMI), hypertension and all-cause mortality in the elderly population in Beijing.

METHODSIn 1992, a cohort of 3 257 people above 55 years old was selected from 3 different areas of Beijing, i.e. urban and suburbs (both plain and mountain areas). Information on physical condition, history of chronic diseases, self-perceived health, as well as smoking and drinking status was collected. Blood pressure, body height and weight were measured among 2 086 of 3 257 subjects.

RESULTSBMI was decreasing with age, while the prevalence of hypertension increased with age. Meanwhile the prevalence of hypertension increased with age as well as with BMI. There was a reverse relationship between BMI and all-cause mortality, i.e. the risk of death was 39.0% lower for those with BMI = 20.0 - 24.9 kg/m(2) (Hazard ratio: 0.61, 95% Confidence interval: 0.49 - 0.75) and 62% lower for those with BMI >/= 25.0 kg/m(2) (Hazard ratio: 0.38%, 95% Confidence interval: 0.29 - 0.49) than those with BMI < 20.0 kg/m(2). After controlling for age, gender, residential place, hypertension as well as self-perceive health status and cognition function, low BMI remained a significant and independent predictor to death.

CONCLUSIONThe distribution of BMI was different in elderly from the youth. The findings suggested that it was important to control the body weight among the elderly hypertensives.

Age Factors ; Aged ; Aged, 80 and over ; Body Height ; Body Mass Index ; Body Weight ; Cause of Death ; China ; epidemiology ; Female ; Humans ; Hypertension ; epidemiology ; physiopathology ; Male ; Middle Aged ; Risk Factors ; Rural Health ; statistics & numerical data ; Sex Factors ; Urban Health ; statistics & numerical data