Heat shock protein (HSP) 105 is a 105x103 stress protein that belongs to the HSP-110 family. It is released by tissues in response to a wide variety of stresses including infection and ischaemia. Studies have shown that the molecule is involved as a biochemical mediator of heat induced apoptosis by binding to p53 at scrotal temperatures and dissociating from it at suprascrotal temperatures in testicular germ cells. Recent studies have shown that HSP-105 is over-expressed in various malignancies besides in normal testicular tissue. HSP-105 may be a candidate of testis antigen.

Surgery in combination with chemotherapy and radiotherapy is the standard of lung cancer treatment,but postoperative recurrence is very common which usually leads to higher mortality and lower life quality. Immunotherapy on post-operative patients fully mobilizes the body's defense mechanisms,activates the immune cells,and kills residual cancer cells. Current research on lung cancer immunotherapy mainly includes four categories:adoptive immunotherapy,dendritic cell vaccine,non-specific antigen immune therapy and anti-gen-specific vaccine. These studies show lung cancer immunotherapy intervention can effectively reduce postop-erative residual cancer cells,reduce postoperative recurrence rate,prolong survival,significantly improve the prognosis,and is worth spreading in clinical practice.

Objective To establish a new method of cutting human pancreases from cadaver donors for islets transplantation, and to discuss the relationship between activity of islets and the cold ischemia preservation time after hypertonic citrate adenine solution perfused.Methods 1500-2000 ml of hypertonic citrate adenine solution was perfused into aorta. Kidey-pancreases-duodenum-spleen were cut together. Digestion of pancreases was done with collagenase P. The islets were isolated, purified and stained. The purity and activity of the islets were measured.Results There was a negative correlation between activity of islets and the cold ischemia preservation time. But there was a positive correlation between activity of islets and the ischemic index.Conclusion The activity of the islets infused with hypertonic citrate adenine solution and degested with collagenase P is high, but the cold ischemia preservation time of the pancreases preserved in hypertonic citrate adenine solution is better not more than 5-6 h.

GROβis a member of the CXC chemokine superfamily.It plays an important role in inflammation and wound healing process.As extensive research continued, researchers realized that the gro gene was one of onco-genes.And its expression product, GROβ, was also found to be very important in angiogenesis, tumorigeness, me-tastasis, and interaction between tumor and immune cells.

Mass spectrometry-based quantitative proteomics has become an indispensable tool for tumor marker discovery.Stable isotope labeling with amino acid in cell culture(SILAC),as a representative of in vivo metabolic labeling strategy,is a simple and relatively quantitative assay for comparative proteomics.Two kinds of cultured cells are grown in the medium containing either a light or heavy isotope labeled essential amino acids.After several cell doublings,the heavy amino acids are introduced into the nascent polypeptides in a sequence-specific fashion and the natural amino acid is completely replaced by its isotopically labeled analog.Accuracy quantification would be feasible by analyzing the peak intensities of every same peptide pairs labeled with light or heavy isotopes by the mass spectrometry.Compared with chemical labeling,SILAC requires much less amount of labeled proteins,and it is an efficient,straightforward,reproducible and accurate approach in cell-based systems.This method can not only investigate the dynamics of protein abundance,but also monitor the variation of posttranslational modifications and protein-protein interactions qualitatively and quantitatively.It is a powerful and important quantitative proteomics tool for tumor marker discovery.

Objective To study the positive rates of oligoclonal bands(OCB)and the IgG index in patients with multiple sclerosis(MS)and in other nervous system disorders(OND).Methods Cerebrospinal fluid(CSF)and serum were collected from 54 patients with MS,271 patients with OND including 62 patients with infections of central nervous system and 209 patients with non-infections.The OCB and IgG index of CSF and serum were detected.Results The positive rate of OCB was 35.2% in MS,8.9% in OND(P<0.05),in which 24.2% in infections of central nervous system(P>0.05),4.5% in non-infections of central nervous system.The positive rate of IgG index was 83.3% in MS,78.2% in OND(P>0.05).Conclusion OCB is helpful to diagnose MS if infection diseases have be excluded,while IgG index can be referenced.

Objective To explore the possible roles of PI3K pathway and MAPK pathway in mediating the survival and differentiation-promoting effect of glial cell line-derived neurotrophic factor(GDNF) on midbrain dopaminergic neurons.(Methods Midbrain) slices of early postnatal rats were cultured,the slices were divided into four groups according to the different substances added into the culture medium: i.e.blank control group,GDNF group,Wortmanin plus GDNF group,and PD98059 plus GDNF group.On the 6th day,some slices were fixed,embedded and sectioned.The sections were processed for tyrosine hydroxylase(TH) immunohistochemistry,and then examined under microscope to determine the morphologic index for statistic analysis(n=6).Meanwhile,some slices were taken for Western blot to examine the expression of TH protein in midbrain slices(n=4). Results GDNF group slices showed the morphologic maturity,the density and the diameter of TH positive neurons,as well as the level of TH expression,were all significantly higher than that of the control groups.The survival effect of GDNF on DA neurons was almost abolished when the PI3K pathway was blocked with Wortmannin;while the MAP kinase and ERK kinase(MEK) inhibitor PD 98059 was added to block the MAPK pathway,the diameter of TH positive neurons was decreased significantly.Conclusion The PI3K pathway might mediate the survival effect of GDNF,while the MAPK pathway seems to be involved in the differentiation process.

Objective To observe the proliferation and differentiation of the neural progenitors in the midbrain substantia nigra(SN) of adult rat when dopaminergic neurons(DA neurons) were destroyed. Methods The Parkinson disease model(PD model) was induced in adult SD rats by steriotaxic injection of 6-OHDA into the right striatum,and then assessed by behavioral analysis to screen the qualified models.After varied survival period,the brain of model rats were perfused and fixed by 4% paraformaldehyde,moved the segment of SN,embedded with paraffin and coronally sectioned continuously.The microsections were processed by immunohistochemistry labeling separately the neural progenitors with anti-nestin monoclonal antibody,the dividing cells with anti-PCNA(proliferation cell nuclear antigen),the neuronal precursors with anti-Tuj1(?-tubulin isotype Ⅲ),and DA neurons with anti-TH(tyrosine hydroxylase).The labeled cells were counted under microscope and analyzed statistically.(Results It) was found in the right SN of PD model rats that: 1.Nestin positive(Nestin~+) cells appeared 10d after 6-OHDA injection,became abundant on 14d,declined in number on 17d,and disappeared on 21d.2.Weakly positive PCNA(PCNA~+) cells appeared on 7d.PCNA~+ cells were abundant on 14d,decreased in number from 21d,with only a few positive cells noticed on 28d.3.Tuj1 positive cells appeared in small number on 10d,became abundant on 14d,decreased in number from 17d and dropped nearly to zero on 21d.4.The number of TH positive neurons was significantly less than the normal control(by 24%) on 7d,and became even less as time elapsed.Conclusion When 6-OHDA is injected into the striatum of adult rats to cause degeneration and death of the DA neurons there,there would be a certain period of time in which a number of neural progenitors will be induced to proliferate actively and differentiate toward neuronal cells(except DA neurons).

Aim To investigate the influences on the aortal structure of rats with long-term high fat forages diet.Methods 14 SD rats were divided into two groups:the control group and the test group. The test rats were fed with high fat forages.12 weeks later, the aortas of the rats were observed with a light microscope, transmission electron microscope(TEM) and scanning electron microscope (SEM).Results In the test group, the aortic tunica intima thickening, endotheliocyte injury and monocyte adhesion were found with a light microscope; the elastic lamina being broken and the smooth muscle cells proliferated. Under TEM, the endothelial cell membrane of the aorta in the test rats was destroyed and appeared to be worm-eclipsed shape.Mitochondria exhibited swelling,vacuole degeneration and its cristae was dissolved, broken or some disappeared.Rough endoplasmic reticula (RER) expanded. The endothelial cell spaces were enlarged and the cell junctions deformed. Monocytes adhering to the endothelial cell stretched out pseudopodia and intruded into the endothelial crevice and the subendothelial layer. Some basement membranes completely sloughed following with endothelial cell. ERE and ribosomes increased in smooth muscle cells. SEM observation showed that the endothelial cells became swelling and the surface of endothelial cell was worm-eclipsed or crater shape. There were deeper crevices between endothelial cells.Conclusions Long-term high fat forages diet can induce injury of the endothelium and elastic lamina, adhesion of the monocytes and its intrusion into endothelial layer and subendothelial layer, proliferation of the subendothelial layer and smooth muscle in the aorta of rats.

Objective:To report and analyze a rare family of sponduloepiphyseal dysplasia congenital(SEDC) in order to supply more resources for genetic bone disease. Methods:Investigation and analysis was performed on a four generation's family of SEDC.Clinic characteristics including X-ray image and chromosome analysis were evaluated.Results:Nine persons suffered from SEDC in this four(generation's) family.The patients presented with same clinical characteristics.The main bone damages affected vertebrae,articulatio coxae,caput femoris and neck. Conclusion:The mode of inheritance of SEDC may be autosomal dominant inheritance.Gene defect during embryonic period may interfere the growth of osteoepiphysis.Further molecular pathologic studies were needed to find the evidence of genetic prognostication of SEDC.