The hospital culture constrction should stick to a human-orientedconcept ,and aim to provide humanitarian brand services.Hospitals should create a favorable hospitalizing condition,and foster a standard treatment,which embodies the feathers of the present time while bearing its own characteristics .It should focus on the in-depth cultural constrction and try to carry out cultural management of the hospital,the leaders of the hospital play a critical role in the cultural constrction

Objective To study the relationship of lung function and the diaphragmatic nerve electrophysiological changes in elderly patients with chronic obstructive pulmonary disease(COPD).Methods The phrenic nerve motor conduction(PNC),diaphragmatic motor evoked potentials(dMEP)and diaphragmatic compound muscle action potentials(dCMAP)were detected in 50 COPD patients and 50 healthy controls aged 60 years and over,respectively. Results (1)The lung function of elderly COPD patients were significantly less than that of control group(P0.05),the lower amplitude of dCMAP than the control(P

Objective To explore the significance of the main effect factors of Th17/Treg for children's gastritis with Hp infection.Methods By prospective study method,40 cases of helicobacter pylori infection related gastritis children were collected as observation group from January 2015 to June 2016,40 cases of children with Hp negative were selected in the same period by physical examination as healthy controls.The main effect factors of Th17/Treg of the two groups were compared.Before and after the treatment,the main effect factors of Th17/Treg of observation group were compared.The main effect factors of Th17/Treg had a correlation analysis.Results The serum IL-17,IL-10 and TGF-beta levels of two groups were compared,and the difference was statistically significant (P ＜ 0.05).Before and after 10 days treatment,serum IL-17,IL-10 and TGF-beta levels of the observation group were compared,and the difference was statistically significant (P ＜ 0.05).Before the treatment,the serum IL-17,IL-10 and TGF-beta levels of observation group had a correlation analysis,and IL-17 and IL-10 were negatively correlated (r =0.778).IL-17 and TGF-beta were also negatively correlated(r =0.767).Conclusion In development process of Hp infection related gastritis for children,the related factors of Th17 and Treg cells involved in the immune pathological process.Immune prevention for children's gastritis with Hp infection provides new train of thought.

Objective: To establish an HPLC fingerprint spectrum of Mongolian medicine Rubus sachalinensis. Methods: An HPLC was performed on the column of phenomsil ODS (250 mm × 4. 6 mm,5 μm) with the mobile phase of 0. 3% phosphoric acid-MeOH with gradient elution at a flow rate of 1. 0 ml·min-1 ,the detection wavelength was 290 nm, the column temperature was 25 ℃and the injection volume was 20 μl. Totally 7 batches of Rubus sachalinensis from different habitats were analyzed. Results:The RSD of relative retention time of the common peaks of Rubus sachalinensis was less than 3%, and that of relative peak areas was below 3%as well, which were in accordance with the requirements of fingerprint. Conclusion:The established HPLC fingerprint has promising accuracy, repeatability and stability, which can be used as one basis for the quality control of Rubus sachalinensis.

Objective To observe the inflammatory state and residual renal function (RRF) of the patients with maintenance hemodialysis treated through Shenkangling. Methods Sixty patients with maintenance hemodialysis were randomly divided into two groups, the observation group (30 cases) and the control group (30 cases). They were treated through hemodialysis and hemodiafiltration. The patients in the observation group were treated with Shenkangling 8 tablets/time, 3 times/day. RRF and urine volume after six months were evalued. Serum high-sensitivity C reactive protein (hs-CRP), tumor necrosis factor alpha (TNF-α), and leukocyte mediated IL-6 (IL-6) before and after treatment were detected. Results There were no statistical differences in RRF and urine volume between the two groups before treatment (P>0.05). After six months, RRF and urine volume in the two groups were significantly reduced (P<0.05), and those of the observation group were significantly higher than those of the control group (P<0.05). There was no statistical significance in serum hs-CRP, TNF-α and IL-6 between the two groups before treatment (P>0.05). After treatment, the serum levels of hs-CRP, TNF-α, IL-6 significantly decreased in the observation group, and lower than those of the control group (P<0.05). Conclusion Shenkangling can protect the residual renal function of hemodialysis patients, and reduce the inflammatory reaction.

To prepare a large amount of pure alive Schistosoma japonicum eggs, rabbit was infected with 2000 cercariae and its liver was taken aseptically 38-45 days after infection and homogenized. The homogenate was screened through different sieves(60, 120, 200, 300, 360 meshes per inch respectively), and washed with 1.2% NaCl. The eggs and leftover were then digested with 0.25% trypsin for 2 hours, sieved over 360 meshes per inch and washed with RPMI 1640 medium. The collected eggs reached to (95.1?6.4)% of live eggs, with a high efficiency.

AIM: To investigate the effects of nitric oxide on ultrastructure and anionic sites of glomerular in renal ischemia reperfusion injured(I-RI) rats.METHODS: Animals were divided randomly into five groups:(1) sham group(n=6);(2) I-RI group(n=6),0.3 mL normal saline was injected via venae lingualis 20 min before ischemia;(3) SNP+I-RI group(n=6),2.5 ?g/kg sodium nitroprusside(SNP) was injected via venae lingualis 20 min before ischemia;(4) AG+I-RI group(n=6),10 mg/kg aminoguanidine(AG) was injected via venae lingualis 20 min before ischemia;(5) L-NNA+I-RI group(n=6),10 mg/kg N~?-nitro-L-arginine(L-NNA) was injected via venae lingualis 20 min before ischemia.Anionic sites of glomerular were studied with a cationic probe-polyethyleneimine(PEI) and ultrastructure was observed under electron microscope in renal I-RI rats.RESULTS:(1) Ultrastructure of glomerular was normal and anionic sites(AS) was located clearly in lamina rare externa of GBM in sham rats.The PEI particles arranged regularly in line(19.3?1.7/(1 000 nm)) under electronic microscope.Obvious foot processes derangement and effacement were observed and the AS number in GBM of I-RI group was fewer(16.6?1.0/(1 000 nm),P0.05).CONCLUSION: Foot process effacement and reduction of anionic sites were present in glomerular filtration membrane in renal I-RI rats.NO aggravated those injuries,indicating that NO plays a role in the ultrastructure damages of glomerular filtration membrane in I-RI rats.

ObjectiveTo evaluate the usefulness of an ultrasonography-based method to examine quadriceps femoris contracting. MethodsThe thickness of bilateral rectus femoris (RF) and vastus intermedius (VI) and the cross-sectional area (CSA) of RF was measured in 10 healthy subjects （n=20） in a relaxed position and 3 isometric contraction states using ultrasonography by two examiners. The date was compared with paired t test. Half of these subjects （n=10） were also measured with MRI at rest. The date was also compared with paired t test. ResultsThere was no significant difference between MRI and static compound ultrasonography, nor between two examiners. There was a significant difference (P<0.05) in thickness of RF and VI between relaxed and isometric contraction conditions. There was no significant difference among isometric contraction conditions. There was no significant difference among measurements of RF-CSA. Conclusionultrasonography shows good validity and reliability in measuring quadriceps shape.

0.05).Compared with SHR group,left ventricular weight mass index decreased significantly in SHR-A group(P

Objective:To study the effect of B7-1 costimulatory molecule in inducing tumor immunity.Methods:The lymphocytes were examined in vitro,for both proliferation induces(PI) and specific cytotoxic activity,H22 mice were challenged subcutaneously either by the mock following which the latency,survival time and tumor mass growth were noted.Results:The H22-B7-1 cells more effectively induced the proliferation of effecter lymphocytes and the generation of specific lytic activity against H22 cells.The H22-B7-1 cells demonstrated slower rate(P