Objective To investigate the significance of detecting the level of CRP,TNF-α,IL-6 and IGF-1 in the patients with type 2 diabetes mellitus(T2DM).Methods Sixty patients with T2DM in the Pingliang Municipal People′s Hospital from February 2013 to March 2015 were selected as the observation group And contemporaneous 60 individuals undergoing healthy physical exami-nation were selected as the control group.The levels of FBG,2 hBG,FINS,HbA1c,CRP,TNF-α,IL-6 and IGF-1 were detected in these two groups.The correlation between CRP,TNF-α,IL-6 and IGF-1 with FBG,FINS and HbA1c were analyzed.Results The levels of FBG,2 hBG and HbA1c in the observation group were significantly higher than those in the control group,the difference was statistically significant (P<0.05).The FINS level in the observation group was significantly lower than that in the control group,the difference has statistically significant (P<0.05).The levels of CRP,TNF-α,IL-6 and IGF-1 in the observation group were (8.46±2.45)mg/L,(18.56±4.23)ng/L,(13.53±3.24)pg/L and (125.33±23.14)ng/L respectively,which were signifi-cantly higher than (3.23±1.02)mg/L,(11.25±3.01)ng/L,(7.24±1.78)pg/L and (98.45±18.56)ng/L in the control group, the difference was statistically significant(P<0.05).CRP,TNF-αand IL-6 were positively correlated with FBG,FINS and HbA1c;IGF-1 was negatively correlated with FBG and HbA1c.Conclusion Detecting the CRP,TNF-α,IL-6 and IGF-1 levels can provide a basis for the diagnosis of T2DM and thinking for its treatment.

Objective To observe the clinical effect and investigate the mechanisms of Yikun Oral Liquid in treating climacteric syndrome due to yin deficiency of liver and kidney. Methods One hundred and three cases of climacteric syndrome of yin deficiency of liver and kidney were observed, including 70 cases of treatment group (treated with Yikun Oral Liquid) and 33 cases of control group (treated with Tibolone tablets). Quantitative studies were performed on the clinical symptoms (using Kupperman marking standard), levels of estradiol (E2), progesterone (P), follicle-stimulating hormone (FSH), luteinizing hormone (LH) and endometrial thickness respectively before and after treatment. Results Clinical symptoms were improved obviously in two groups after treatment (P

Medical workers are the main designers and participants of clinical trials.In this consideration,popularization of good clinical practice (GCP)knowledge among them can improve their execution of trials,standardize basic clinical trial requirements,protect legitimate rights and health/safety of the subjects,and make the trial results more reliable.These objectives,if accomplished,will upgrade the clinical trials competency of medical institutions in China,and encourage healthy growth of the medical and pharmaceutical industry.The paper probed into the occupation qualification system of clinical trial workers and their supervision mechanism,inclusion of GCP into the continuing education system.

0.05).Compared with SHR group,left ventricular weight mass index decreased significantly in SHR-A group(P

Objective To understand the actual situation of community‐based diabetes management and analyze common prob‐lems in diabetes management and find the solutions to diabetes management though the objective investigation .Methods The pa‐tient′s data including gender ,age ,education ,medication ,lifestyle information were collected by household face‐to‐face survey and were performed a single factor χ2 analysis of relevant indicators .Results Relative to the patients with middle school education only and is less than 3 000 a month ,the diabetic patients who ere above the junior middle school and family income equal to or greater than 3 000 had good diabetes management on medication ,diet ,exercise ,blood glucose monitoring ,diabetes related knowledge .Con‐clusion Patient′s education and economic level could effect attitudes toward diabetes ,medication ,diet ,exercise ,blood glucose moni‐toring ,diabetes‐related knowledge .

Purpose To investigate the significance of PET/CT in differential diagnosis of incidental thyroid benign or malignant lesions with high fluorodeoxyglucose (FDG) uptake.Materials and Methods Forty cases with focal high FDG uptake lesion in thyroid accidently detected by PET/CT were retrospectively collected. The subjects were dived into physiological uptake group (n=18), benign lesion group (n=16) and malignant lesion group (n=6) according to the pathology or follow-up examinations. The average SUVmax of different groups was analyzed, and ROC curve was used todetect benign or malignant lesions.Results The average SUVmax was 3.7±1.1 in physiological uptake group, 4.9±2.3 in benign lesion group and 9.5±4.8 in malignant lesion group. Significant difference was noticed among the three groups. The average SUVmax in malignant lesion group was higher than that of the other two groups with statistical difference (P<005). No significant difference was observed between physiological uptake group and benign lesion group (P>0.05). ROC analysis showed that the sensitivity and specificity for thyroid malignant tumor were 100.0% and 87.2% at SUVmax of 8.5.Conclusion18F-FDG PET/CT plays an important role in differentiating benign lesions of thyroid from malignance, but it is not helpful to identify benign lesions from physiologic uptake.

Objective To investigate the satisfaction of clinicians on clinical research associates (CRA)and its influencing factors,for the purpose of providing rationalized proposals on education of CRAs.Methods 141 clinicians were randomly sampled from tertiary hospital for questionnaire survey,using the 5-point Likert scale.The survey covers 4 levels,i.e.,the work attitude,professional knowledge and ability,communication skills,and project management capabilities,as well as 14 dimensions.Data processing and statistics were analyzed using SPSS 15.0 software.The attribute characteristics of the investigation subjects were analyzed using x2 of the contingency table,along with analysis of its correlation with the general satisfaction on CRAs.Results The mean values of the 12 indicators range 2.28 to 3.75,with low satisfaction in general.Among these indicators,satisfaction of the service attitude of the CRA,and of their familiarity with the pilot program and CRF completion axe the highest,respectively,74.04％and 61.70％.Satisfaction of the rest 10 indicators falls below 50.00％.The chisquare analysis showed no association between satisfaction and gender,education,job titles.The satisfaction is different(P＜0.05)between those trained and those not,while there exists a significant differences(P＜0.01)between those participating in different number of tests.Conclusion Clinicians have a low satisfaction on CRAs.It is recommended to strengthen the training,establish a CRA occupation certification system,and to strengthen the clinicians' emphasis and competency of clinical trials.These actions will normalize the industry of clinical trials and improve the level of clinical trials in China.

AIM: To get the IR spectrums of chrysanthemums from the different producing areas,and to find out the characters of IR spectrums,and the data of IR spectroscopy of chrysanthemums from the different producing areas. METHODS: Using Fourier transform infrared spectroscopy. RESULTS: The FTIR spectrums、Second-order derivative spectrums、Two-dimensional spectrums of chrysanthemums from the different producing areas have their obvious IR characters. CONCLUSION: We can discriminate chrysanthemums from the different producing areas macroscopically and holistically by IR spectroscopy.IR spectroscopy can give us the digital descriptions of TCM,so it is a new analytical method to discriminate TCM.

AIM: To get the IR spectrums of the different extracted parts of chrysanthemum, and to find out the characters of IR spectrums. METHODS: To use Fourier transform infrared spectroscopy. RESULTS: The FTIR spectrums of the different extracted parts of chrysanthemum had their obvious IR characters. CONCLUSION: IR spectroscopy can give us the digital descriptions of TCM, so it is a new and scientific analytical method to discriminate TCM.

Objective To express and purify the Schistosoma japonicum adenylate kinase(AK) protein of which the cDNA sequence was subcloned into the pET32a(+) soluble expression plasmid, and to evaluate its immunoreactivity. MethodsThe recombinant plasmid was transformed into {E.coli} BL21, and induced with IPTG for expression. The bacterial lysis was conducted by ultrasonication and the supernatant was analyzed by SDS_PAGE after boiling and centrifugation. The target protein was purified with the Ni_NTA agarose. The proteins on the gel were transferred to the PVDF film and then the immunoreactivity was tested by Western blotting using anti_6_His antibody, sera from rabbits 6 weeks after infected with cercariae or UV_attenuated cercariae. The purified protein was coated for ELISA to test the cercariae infected rabbit serum and the normal rabbit serum. Results The molecular weight of the target fusion protein was {Mr 40 000} after being induced with IPTG. The fused protein showed a single band when reacted with anti_6_His antibody, the cercariae infected rabbit serum and attenuated cercariae infected rabbit serum. Conclusion The AK protein is expressed as a fused protein with thioredoxin and its molecular weight is about {Mr 40 000}. This protein has a positive immunoreactivity with sera of rabbits infected with cercariae and UV_attenuated cercariae.