Objective To study the effects of exerci se on heart rate variability (HRV) in elderly patients with old myocardial infarct ion (OMI). Methods Fourty-three patients were recrui ted and divided into an exercise group (24 cases) and a control group (19 cases) . All the patients were given conventional treatment, with those in the exercise group being administered with adequate exercise in addition. Twenty-four hour Holter taper and ultrasonic cardiogram were performed before and at the end of t he 1 month exercise program, respectively, for HRV analysis and cardiac function assessment. Results Before the exercise,HRV and card iac function of senile patients with OMI were significantly lower than those of the controls( P 0.05). Conclusion Exercise can sign ificantly improve the cardiac function of elderly patients with old myocardial i nfarction.

Objective To investigate the neuropsychological characteristics of secondarily generalized tonic clonic seizures (SGTCS). Methods Twenty patients of SGTCS were divided into higher SGTCS group (the times of SGTCS ≥ 20,10 cases) and lower SGTCS group (the times of SGTCS < 20,10 cases). Investigated the tests of verbal intelligence quotient (VIQ), procedure intelligence quotient (PIQ) and full-scale intelligence quotient(FIQ), as well as cognitive test, BCRT-reaction time, CVST-reaction time and the deterioration scores. Results The VIQ, PIQ, FIQ and cognitive test of higher SGTCS group were significantly lower than those in lower SGTCS group[(94.9 ± 20.9) scores vs (116.0 ± 17.8) scores, (95.3 ±16.8) scores vs(117.8 ± 12.4) scores, (94.3 ±20.6) scores vs (118.0±12.8) scores, (14.1±4.9) scores vs (19.8 ± 2.1)scores] (P < 0.05 or < 0.01), and the outcomes of CYST-reaction time and the deterioration scores were significantly higher than those in lower SGTCS group [(16.6 ± 5.1) s vs (11.9 ± 2.0) s, (3.1 ± 1.4) scores vs(1.6 ± 1.1) scores](P < 0.05). Conclusion Intellectual and information gathering capability of SGTCS were deteriorated with the degree of disease worsened.

To explore the distribution of the bitter principle ganoderic acid B in different parts of Ganoderma lucidum (Leyss. ex Fr.) Karst.. Methods　Ganoderic acid B in epidermis, phellem, stipe, spore and pileus were determined by RP-HPLC. Results　Ganoderic acid B was found to be mainly concentrated in the entire surface of the basidiocarp, other parts contained much less amount. Conclusion　Result of the study may provide references for the search of such active principle from this fungus.

Objective To lay a foundation for the cultivation of disease-free plantlet of Citrus medica L.var.sarcodactylis Swingle(CMS) by discussion of the factors affecting survival rate for micro-grafted shoot-tip of CMS.Methods With citrus limon seedlings cultured in the dark as the rootstock,shoot-tip of CMS was grafted to the inverse T-shaped cut in the cotyledon of rootstocks.Then an investigation was carried out on the factors which affected the survival rate for micrografted shoot-tip of CMS.The factors included age of rootstocks,scion size,culture medium with different concentrations of phytohormones,and rootstocks with or without cotyledon.Results The survival rate for micro-grafted shoot-tip of CMS was closely related to the factors mentioned above.A higher survival rate was obtained when CMS shoot-tip with 4 pieces of leaf primordia was grafted to the inverse "T" shaped cut in the cotyledon of parental stock(citrus limon seedlings) which was cultured in the dark for 14 days,and when the citrus limon seedlings were cultivated in MT-based medium with ?-naphthylacetic acid 0.1 mg?L-1 added.Conclusion The optimized micro-grafting conditions for CMS shoot-tips improve the propagation of micro-grafted plantlet and the survival rate of micro-grafted shoot-tip of CMS.

Objective To provide a new approach of preservation for germplasm resources of Citrus medica var.sarcodactylis(CMS).Methods A micrografting test was made on CMS shoot-tips after vitrified cryopreservation,resulting in living shoot-tip.Results First the CMS shoot-tip is inoculated in medium consisting of MS,5%DMSO,and 5%sucrose for 48 h preincubation.Then the shoot-tips were cut off 3—4 mm long and fore-treated by 60%PVS_2 at room temperature(25℃) for 30 min.After that,they were treated by PVS_2 at 0℃for another 80 min and conserved in liquid nitrogen for 24 h.Next the shoot-tips were defrosted by aqueous bath at 40℃and cleaned twice at room temperature(25℃) by MT minimal medium with additive 1.2 mol/L sucrose,10 min once.Finally data collected recorded a higher survival rate of 81.25%by TTC inspectation and a better regeneration rate of 52.94%if the CMS shoot-tips had been grafted on darkly-cultured parental stock,growing,and differentiating normally afterwards.The plantlets detected by polymerase chain reaction(PCR) showed that the pathogens of Huanglong disease had been removed.Conclusion Therefore a conclusion is made that the approach of vitrified cryopreservation may be applied to the preservation of CMS germplasm resources.

Objective To observe the influence of sowing time and planting density on seed germination percentage and growth of Desmodium styracifolium(Osb.) Merr..Methods After quality inspection of the seed,the seeds planting test for Desmodium styracifolium(Osb.) Merr.was carried out from March to October,and the influences of sowing time and planting density on the seed germination percentage and growth were observed.Results On the planting base of Pingyuan county in Guangdong province,the seeds of Desmodium styracifolium(Osb.) Merr.planted from March to October had a high germination percentage and the seedling grew well.With the increase of planting density,the seed germination percentage decreased,but was up to 80% when the planting density was 0.5～1.0 g /m2.Conclusion The optimum planting time for Desmodium styracifolium(Osb.) Merr.is in autumn and the optimum planting density is 0.5～1.0 g /m2.

Objective: To explore childhood traumatic experiences of OCD patients who were also diagnosed personality disorders (PDs) and those without PDs. Methods:SCID-I/P was administrated to diagnose OCD patients. PDQ+4 and PDI-IV were then administrated to 44 OCD subjects to determine whether they also suffered from personality disorders. Childhood maltreatment experiences of the sample were measured with Childhood Trauma Questionnaire (CTQ).50 normal control were also collected and subjected to CTQ-SF tests. Results:32 of 44 (72%) OCD patients also met DSM-IV criteria for personality disorders. OCD patients scored significantly higher than normal control in CTQ-SF (Childhood Truma Questionuaire-28 Short Form) and in all domains with the exception of physical abuse. Comparison between OCD subjects with PDs and normal control, the former scored significantly higher in emotional abuse (55.10/32.54), sex abuse (49.63/36.30), physical neglect (52.03/34.76), and emotional neglect domains (55.84/32.32). Whereas OCD subjects without PDs only scored significantly higher in emotional neglect domains compared with normal control (42.50/28.26).Conclusions:Subjects with PDs experienced more severe traumatic events during their early life. "pure OCD" patients, however, might experienced more emotional neglect.

Objective: To establish the method of fingerprint analysis on volatile oil of Pogostemon cablin (Blanco) Benth. cultivated in Shipai country by GC MS, and to study the main characteristic components. Methods: The main components of the volatile oil of Pogostemon cablin (Blanco) Benth. from different colletion time in Shipai country were determined by GC MS, and compared with the volatile oil of “Gaoyao huoxiang” and “Hainan huoxiang” cultivated in the same GAP plots. Results: RSD of precision and reproducibility and stability is in the range of 5%, and the main components of the volatile oil of “Shipai huoxiang” from different collection time are nearly similar, and the content of Pogostone increases along with cultivation time, and the content of Pogostone and Patchoulic alcohol are significantly differences compared with the “Gaoyao huoxiang” and “Hainan huoxiang”. Conclusion: The method is reliable, accurate and can be used for quality control of volatile oil of Pogostemon cablin (Blanco) Benth.

Objective To develop a stable and quick method to determine the urea in swimming pool water.Methods On the conditions of strong acid and calefaction,urea can react on antipyrine and diacetyl monoxime and become yellow which can be determined by colorimetric analysis in 450 nm with the ALLIANCE FUTURA continuous flow injection auto analysis system.Results Determined by this method,the linear range was 0.5-4.0 mg/L,the regression equation was y=0.077 5x+0.021 9,the correlative coefficient r=0.999,the detection limit was 0.07 mg/L.With residual chlorine of 0.3 and 3.0 mg/L,the recovery rates were 98.8%-104.0% and did not disturb the determination.The results determined by the present method were the same as those by the method in GB/T 18204.29-2000(Wilcoxon Signed Ranks Test:Z=0.051,P=0.959).Conclusion Determination of the urea in swimming pool water with continuous flow injection auto analysis,the sensitivity,accuracy and limit of determination can meet to the requirement,moreover,the analysis speed is faster,less reagent will be needed,the present method can be generalized in the laboratory that has the conditions.