1.Effects of Platelet-Activating Factor Receptor Antagonist SRI 63-441 on the Changes of Lipoperoxidation, Lysoenzyme Release and Extravascular Lung Water in Canine Endotoxic Shock
Honghong ZHANG ; Shaohua LI ; Zhongli WU
Academic Journal of Second Military Medical University 1982;0(01):-
We investigated whether platelet-activating factor (PAF) mediates endotoxin-induced changes of lipoperoxidation, lysosomal enzyme release and increased extravascular lung water content in dogs using a specific PAF receptor antagonist, SRI 63-441. Endotoxin infusion caused an increase in serum malonyldialdehyde (MDA) from baseline (100%) to 130.04?14.00% and 169.16?32.49% and ?-glucuronidase (?-g) activity to 191.05?86.71% and 242.54?49.09% at 3 and 6h, respectively. The bronchoalveolar lavage fluid (BALF) ?-g activity, lung MDA and extravascular lung water also significantly increased (P
2.Literature Review on Adverse Events Following Immunization for Influenza Virus Vaccine
Honghong BAO ; Li LI ; Wendi WU
Chinese Journal of Vaccines and Immunization 2008;0(06):-
To evaluate security of influenza virus vaccines by analysis of adverse reaction to influenza virus vaccine,especially the relationship between Guillain Barr? syndrome and influenza vaccines.
3.Relationship between VDR gene polymorphism and BMD in elderly male patients with COPD
Xiaofen MU ; Honghong ZHANG ; Qing WU ; Yazhuo HU ;
Chinese Journal of Geriatrics 2003;0(11):-
Objective To investigate the association between the genetic polymorphisms of vitamin D receptor(VDR)and bone mineral density (BMD) in elderly male patients with chronic obstructive pulmonary diseases. Methods Pulmonary function, body mass index(BMI), serum calcium, serum phosphorus and bone glaprotein were determined respectively in COPD group and control group. In the two groups the VDR gene polymorphisms were analyzed by polymerase chain reaction and restriction fragment length polymorphism (PCR RFLP) and BMD were investigated by dual energy X ray bone mineral density. Results There were differences in BMD, BMI and bone glaprotein between the two groups. The BMI, bone glaprotein and BMD of femur neck in COPD group were(22 65?3 18)kg/m 2 ,(2 50?0 57) ?g/L and(0 75?0 13)g/cm 2 respectively, which were more decreased than those in control group: BMI (24 86?3 68)kg/m 2 , bone glaprotein (2 87?0 61) ?g/L and BMD of femur neck (0 86?0 12) g/cm 2 ( P
4.The relationship between soluble CD147 and the risk factors of atherosclerosis in rheumatoid arthritis
Honghong YUE ; Ping ZHU ; Nan LENG ; Zhenbiao WU ; Hongming LI
Chinese Journal of Rheumatology 2008;12(8):515-518
Objective To study the relationship between soluble CD147 (sCD147) level in peripheral blood and serum lipid level and explore the effect of sCD147 on atherosclerosis in rheumatoid arthritis (RA). Methods The level of sCD147 in 36 patients with RA,36 patients with coronary artery disease (CAHD) and 30 healthy volunteers was detected by enzyme linked immunosorbent assay (ELISA) .The disease activity score (DAS28) in RA patients was evaluated and the correlation between sCD147 level and DAS28 score was analyzed.The serum lipid level of RA patients was detected by an automatic biochemical analyzer and the cor relation between sCD147 level and serum lipid level was analyzed.Results The level of sCD147 in serum of RA patients was significantly higher than that in patients with CAHD and healthy volunteers,sCDI47 level in the RA group with high DAS28 score was significantly higher than that with low or medium DAS28 score.In RA patients,elevated total cholesterol (TC) and triglyceride (TG) level was positively correlated with serum sCDI47 level (r=0.84,P<0.05;r=0.87,P<0.05;while slightly elevated,normal TC and normal TG had no correlation with serum sCDI47 level (r=0.41,P=0.21;r=0.14,P=0.57;r=0.49,P=0.87).Elevated or slight ly elevated LDL-C was positively correlated with serum sCD147 level (r=0.86,P<0.05;r=0.81,P<0.05), while no correlation could be found in the group with normal LDL-C level (r=0.78,P=0.22).The high density lipoprotein-cholesterol (HDL-C) level decrease in RA patients had no correlation with serum sCD147 level (r--0.04,P=0.96;r=0.13,P--0.87).Conclusion sCD147 may be involved in the pathogenesis of RA and associate with disease activity.Elevated sCD147 level may be associated with abnormal serum lipid in RA.
5.The effects of constraint-induced movement therapy on early stage upper limb function recovery in patients with subacute stroke
Honghong HUANG ; Lingxing WANG ; Quanxiang ZHANG ; Lingfeng WU ; Ruoting LIN
Chinese Journal of Physical Medicine and Rehabilitation 2014;36(11):838-841
Objective To investigate the effects of constraint-induced movement therapy (CIMT) on the function of hemiplegic upper extremity in the early subacute stroke patients.Methods A prospective,single-blinded,randomized controlled study was conducted.Forty-seven stroke patients with hemiplegic upper limb dysfunction were randomly divided into 2 groups:a CIMT group and a control group.The CIMT group received constraintinduced movement therapy and the control group was treated with conventional rehabilitation therapy.Both groups were treated 3 h daily,5 d a week for 2 weeks.The Wolf motor function test (WMFT) and Fugl-Meyer Assessment (FMA) were used to evaluate upper extremity motor function,and motor activity log (MAL) was used to assess upper extremity ability in activities.WMFT,FMA and MAL were measured before and after 1 day and 6 months of treatment.Results After 1 day of treatment,FMA,WMFT,MAL-AU and MAL-HW were 53.81 ± 2.59,66.68 ± 3.54,1.89 ± 0.88 and 3.26 ± 0.65,respectively,in the CIMT group,and 48.61 ± 4.48,62.10 ± 7.97,1.25 ± 0.64 and 2.65 ± 0.93,respectively,in the control group.After 6 months of treatment,FMA,WMFT,MAL-AU and MAL-HW were 57.53 ±2.01,69.57 ± 3.00,3.00 ±0.82 and 3.84 ±0.69,respectively,in the CIMT group,and 53.30 ± 2.88,66.20 ±3.59,2.20 ± 1.06 and 3.25 ±0.64,respectively,in the control group.The scores of FMA,WMFT,MAL in the CIMT group were all higher than those in the control group at 1 day and 6 months post-treatment,and the differences were statistically significant (P < 0.05).Conclusion Constraint-induced movement therapy can significantly improve the patients' hand function in the early stage of subacute stroke,which maintain up to 6 months of follow-up.
6.Dynamic Analysis on Production and Marketing of Bruceae Fructus
Haiyan LI ; Yuxin PANG ; Quan YANG ; Xuan HU ; Lifen WU ; Honghong WU
Chinese Journal of Information on Traditional Chinese Medicine 2016;(1):15-17,18
Pharmacological studies have shown that Bruceae Fructus has unique antitumous effects, which has broad market prospects. In recent years, the demand of Bruceae Fructus has increased year by year. Production and marketing thrive, and prices continue to rise. Therefore, it has become one of the Chinese medicinal herbs with antitumour effects that obtained people’s attention. This author summarized the dynamic condition of production and marketing of Bruceae Fructus during the last 10 years, and analyzed the major reasons for price changes and forecast development tendency so as to provide references for the reasonable layout of production and marketing of Bruceae Fructus.
7.Effects of Huang-Lian-Jie-Du-Decotion containing serum on expressions of autophagy related gene in macrophages
Honghong YU ; Mali WU ; Zhiwei ZHANG ; Rui CHEN ; Yun WU ; Zhixuan DUAN ; Weiyi TIAN
Chinese Journal of Immunology 2016;32(8):1150-1152,1164
Objective:To investigate the effects of Huang-Lian-Jie-Du-Decotion containing serum for the autophagy of RAM264.7 macrophages ,and to explore its possible anti-atherosclerosis mechanism .Methods:The SD rats were intervened with large , middle and small dose Huang-Lian-Jie-Du-Decotion to prepare containing drug serum .Cell proliferation was tested by MTT assay .The gene and protein expressions of Beclin 1, mTOR was assessed by fluorescent quantitative PCR and Western blot method .Results:Compared with the normal serum group ,Huang-Lian-Jie-Du-Decotion containing serum enhanced expression of the mRNA and protein level of Beclin1,and decreased the expression of mTOR and p-mTOR(P<0.05 or P<0.01).Conclusion:Huang-Lian-Jie-Du-Decotion containing serum could regulate the expressions of Beclin 1 and mTOR.These might be one of the mechanisms for preventing atheroscle-rosis.
8.Feature extraction of motor imagery electroencephalography based on time-frequency-space domains.
Yueru WANG ; Xin LI ; Honghong LI ; Chengcheng SHAO ; Lijuan YING ; Shuicai WU
Journal of Biomedical Engineering 2014;31(5):955-961
The purpose of using brain-computer interface (BCI) is to build a bridge between brain and computer for the disable persons, in order to help them to communicate with the outside world. Electroencephalography (EEG) has low signal to noise ratio (SNR), and there exist some problems in the traditional methods for the feature extraction of EEG, such as low classification accuracy, lack of spatial information and huge amounts of features. To solve these problems, we proposed a new method based on time domain, frequency domain and space domain. In this study, independent component analysis (ICA) and wavelet transform were used to extract the temporal, spectral and spatial features from the original EEG signals, and then the extracted features were classified with the method combined support vector machine (SVM) with genetic algorithm (GA). The proposed method displayed a better classification performance, and made the mean accuracy of the Graz datasets in the BCI Competitions of 2003 reach 96%. The classification results showed that the proposed method with the three domains could effectively overcome the drawbacks of the traditional methods based solely on time-frequency domain when the EEG signals were used to describe the characteristics of the brain electrical signals.
Algorithms
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Brain
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physiology
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Brain-Computer Interfaces
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Electroencephalography
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Humans
9.Effects of viola-containing serum on phagocytosis and TOLL-like receptor expression of macrophage
Zhiwei ZHANG ; Kun CAI ; Mali WU ; Honghong YU ; Ling LENG ; Wenpeng YUE ; Weiyi TIAN
Chinese Journal of Immunology 2014;(6):759-762
Objective:To investigate effects of viola on macrophage TOLL-like receptor expression ,and tentatively explore the partial mechanism of viola intervention on macrophage function.Methods: Using viola water decoction lavage intervention in clean grade SD rats of conventional preparation containing serum ,In a certain concentration coculture with mouse peritoneal macrophages in 96-well plates.After 24 hours,cell phagocytosis activity was determined by neutral red uptake assay ,changes in expression levels of TLR-1,TLR-2,TLR-3,TLR-4,TLR-5 mRNAs were determined by RT-PCR.Results:Compared with the same concentration of normal serum group :(1 ) viola-containing serum group macrophage activity was significantly increased ( P<0.01 );( 2 ) Within certain concentration,viola containing serum group with increased or decreased expression levels of TLR -1,TLR-2,TLR-3 mRNA(P<0.05 or P<0.01 ) ,the expression level of TLR-4 was not significantly altered in each group ( P>0.05 ) ,and the expression level of TLR-5 was significantly increased in each group ( P<0.01 ).Conclusion:Viola-containing serum has obvious promoting effect on macrophage phag-ocytosis,the mechanism of which may be related to that this drug regulates and controls part of macrophage TOLL -like receptor expres-sion.
10.A new caffeate compound from Nardostachys chinensis.
Yingpeng CHEN ; Zhongping WANG ; Honghong ZHENG ; Yantong XU ; Yani ZHU ; Peng ZHANG ; Honghua WU
Acta Pharmaceutica Sinica 2016;51(1):100-4
A new caffeate compound, (E)-erythro-syringylglyceryl caffeate (1), was isolated from the roots and rhizomes of Nardostachys chinensis Batal., together with nine known phenolic compounds, including (+)-licarin A (2), naringenin 4', 7-dimethyl ether (3), pinoresinol-4-O-β-D-glucoside (4), caraphenol A (5), Z-miyabenol C (6), protocatechuic acid (7), caffeic acid (8), gallic acid (9) and vanillic acid (10). Their chemical structures were elucidated on the basis of spectroscopic data and physicochemical properties. Furthermore, this is the first report of compounds 2, 5 and 6 from Nardostachys genus.