Objective To find the inpact(s) of Losartan on myocardial apoptosis in rats with heart failure. Methods Eight-week-old male SD rats were divided into control group (group C) , heart failure (group HF) and treatment group (group LS). Use of doxorubicin injection model, group LS at the same time in a row to the oral losartan, myocardial ultrastructure was examined by TEM. DNA-situ terminal labeling (TUNEL method) was used to detect myocardial apoptosis and myocardial BAX, BCL-2 protein expression was detected by immunohistochemistry. Results Group HF showed significant myocardial cell injury, and apoptotic bodies were found, serum CPK, CK-MB and LDH were increased. In LS group, myocardial apoptosis index was lower (P <0. 01) , myocardial cells BCL-2 expression, BAX expression reduced as compared with those in HF group. Conclusion Losartan can effectively inhibit the process of heart failure occurred in cardiomyocyte apoptosis, reverse myocardial damage and improve the prognosis of heart failure.

Objective To find the inpact(s) of Losartan on myocardial apoptosis in rats with heart failure. Methods Eight-week-old male SD rats were divided into control group(group C),heart failure(group HF) and treatment group(group LS). Use of doxorubicin injection model,group LS at the same time in a row to the oral losartan,myocardial ultrastructure was examined by TEM. DNA-situ terminal labeling (TUNEL method) was used to detect myocardial apoptosis and myocardial BAX,BCL-2 protein expression was detected by immunohistochemistry.ResultsGroup HF showed significant myocardial cell injury,and apoptotic bodies were found,serum CPK,CK-MB and LDH were increased. In LS group,myocardial apoptosis index was lower (P

Objective To confirm the most effectively screening conditions by comparing the ARR values of different posi‐tions after saline load test .Methods After exclusion the other secondary hypertension ,the completion of saline dressing test ,84 ca‐ses diagnosed hypertensive patients under saline load test results were divided into primary aldosteronism group (pH ,52 cases) and primary hypertension group (32 cases) .Analyzed plasma aldosterone (PAC) ,renin (PRA) values of upright and decumbent body positions ,their ARR values were calculated and the urine aldosterone was tested .Results Those whose upright position ARR rati‐os higher than 240(pg/mL)/(ng · mL -1 · h-1 ) were accounted for 80 .77% (42/52) and 43 .75% (14/32) ,respectively in the both PA group and PH group;Those whose upright position ARR ratios higher than 240(pg/mL)/(ng · mL -1 · h-1 ) and decumbent position PAC higher than 150 pg/mL were accounted for 65 .39% (34/52) and 3 .13% (1/32) ,respectively in the both PA group and ph group;The specificity of this method for PA was 96 .88% .Those whose upright position ARR ratios higher than 240(pg/mL)/(ng · mL -1 · h-1 ) and decumbent position PAC higher than 150 pg/mL and urine aldosterone higher than 10 μg/24 h ,The specificity of this method for PA was 100 .00% .Conclusion It is an effective screening method for primary aldosteronism when ARR values of upright position higher than 240(pg/mL)/(ng · mL -1 · h-1 ) ,PAC of decumbent position higher than 150 pg/mL and urine aldosterone higher than 10 μg/24 h .

To explore the clinical ,pathological and immunohistochemical features of carcinoma with multi-directional differentiation derived from junction of bladder and prostate .We collected clinical data and tissue samples of three typical cases of carcinoma from our hospital .Routine preparation of slides and immunohistochem-ical methods combined with clinical data and pathological changes analysis were adopted .We found that all 3 ca-ses occurred in the elderly lesions involving the bladder and prostate .Dysuria was the main common symptom .All the cases had a history of chronic inflammation in urethral and prostate gland .The following up data showed that survival time in two of them were no more than 15 months.The third patient without chemotherapy who took drugs of bicalutamide,enantone and pamidronate still was alive after 28 months.The pathological changes of these cases had the common features with diversity .They all showed the structure with nests ,papillary,solid,adenoid,single or syncytial cells .The nuclear of cancer cell was enlarged with hyperchromatic feature .The mitotic figures were easily found.The metaplasia and atypical hyperplasia of prostate were all found .The immunohistochemistry results showed positive results for HCK,LCK,p53,p63,PSA,P504S and negative for vimentin and S -100.The average proliferation index of Ki-67 was 77%.

0.05 ) between two groups. The side effects were 7.7 % and 13.3 % in treatment group and control group, respectively. CONCLUSION: The combination of CLA+CBS+Met for 1 wk is an effective and safe treatment for eradication of HP infection with recurrent abdominal in children.

，and 17.47%.Conclusions Xylitol can lower the blood glucose a littte but without significant difference.It has little effect on blood glucose variability of patients with type 2 diabetes mellitus and can be safely used for rehydration.

BACKGROUND: Changes in the center of body weight during standing reflects the structure, function and muscles of the lower limbs and even the whole body. The relationship between standing posture control and foot type, balance ability, and muscle activation is rarely reported. OBJECTIVE: To investigate the effects of natural, lean forward and lean backward standing on foot arch, body balance and muscle activation. METHODS: Fifteen subjects without foot lesions and diseases related to foot function were selected. Three-dimensional foot scanner was used to test and analyze the foot arch in three standing postures. BTS FREEMG 300 surface electromyography tester and balance tester were used to test and analyze the maximum root mean square of muscle and two balance ability indexes (envelope area and average velocity of center of gravity) in three standing postures. RESULTS AND CONCLUSION: (1) For height index of foot arch: there was significant difference between natural standing and forward standing (P ＜ 0.05). There was significant difference between natural standing and backward standing (P ＜ 0.05), but there was no significant difference between forward standing and backward standing (P＞ 0.05). (2) The degree of muscle activation was as follows: when standing naturally, the medial peroneal muscles＞ erector muscles＞ rectus abdominis＞ gluteus medius＞ biceps femoris＞ external oblique femoris＞ anterior tibial muscle＞ rectus femoris muscle＞ gluteus maximus muscle. During forward standing: the medial of the peroneal muscle＞ medius gluteus＞ vertical ridge＞ long head of biceps femoris＞ anterior tibial muscle＞ rectus abdominis muscle＞ gluteus maximus. During backward standing: the obliquus externus femur＞ rectus femoris＞ long head of biceps femoris＞ erector spinae＞ musculus rectus abdominis＞ the medial of the peroneal muscle＞ gluteus medius＞ gluteus maximus. (3) Balance: there was no significant difference between natural standing and forward standing (P＞ 0.05); there was significant difference between natural standing and backward standing (P ＜ 0.05), but there was no significant difference between forward standing and backward standing (P＞ 0.05). (4) In summary, it is easy to maintain body stability at low muscle activation intensity when the human body is standing naturally. During the body backward standing, the muscle activation intensity is highest due to instability. When the center of gravity moves forward and backward, the contralateral muscle is mainly activated. The arch of the foot rises when it leans forward and backward. The slight anterior and posterior movements of the center of gravity in the standing posture can lead to changes in muscle activation degree and balance control.

This article summarizes overview of studies on the effect and mechanism, clinical application, adverse effect of Hongsheng Dan and its preparations and monitoring on them, defining the effect of Hongsheng Dan's preparations in clinical application and pointing out its note points and monitoring indicators during applications, in order to define the direction for future studies on the preparations.
Drugs, Chinese Herbal ; adverse effects ; pharmacology ; therapeutic use ; Humans ; Medicine, Chinese Traditional ; trends

Objective To investigate the diagnostic value of the partial least square discriminant analysis (PLS-DA) model based on seven serum cytokines for children patients with mycoplasma pneumonia,the 7 cytokines includ interleukin-2 (IL-2),IL-4,IL-6,IL-8,IL-10,tumor necrosis factor alpha (TNF-α) and interferonγ(IFN-γ).Methods Serum levels of cytokines were measured by the double antibody sandwich method of ELI-SA in 140 patients with pneumoniae infection and 135 normal healthy controls,and data was analyzed with the receiver operation characteristic (ROC) curve and the PLS-DA.Results The area under the ROC curve (AUC) of serum IL-10 and IFN-γfor the diagnosis of MPP was 0.84 (95％ CL:0.79 ～ 0.89).The sensitivity of IL-10 and IFN-γ for the diagnosis of MPP was 91.4％ and 82.1％,with the specificity was 77.0％ and 82.2％,respectively.The diagnostic accuracy of the PLS-DA model based on seven serum cytokines for children MPP and the controls was 90.0％ and 88.15％,and the prediction accuracy was 86.4％ and 87.4％,respectively.Conclusion Seven serum cytokines based on PLS-DA model was helpful for the diagnosis of children patients with MPP.

BACKGROUNDMalignant pleural effusion is one of the common complications in patients with advanced lung cancer. Intracavitary injection of drug is a usual method, but it also exhibits unstable efficacy and obvious adverse reaction. The aim of this study is to evaluate the efficacy of hyperthermia combined with intracavitary injection of drug for malignant pleural effusion.

METHODSFifty patients with malignant pleural effusion caused by lung cancer were randomized into two groups after puncture or closed drainage. Group A was treated with hyperthermia combined with intrapleural injection of cisplatin and interleukin-2. Group B was treated only with intrapleural injection of cisplatin and interleukin-2.

RESULTSThe response rate of pleural effusion was 88% in group A and 60% in group B (P=0.024). The quality of life in group A was significantly better than that in group B (P=0.013).

CONCLUSIONSHyperthermia combined with intracavitary injection is effective and secure in treatment of malignant pleural effusion.