Objective To study urethral epithelium cu lt ure in vitro as a basic role in the further study on tissue engineering urethra. Methods A bit of urethral mucosa was taken from a young male New Zealand hare and was digested by enzyme into single cell liquid.The ce ll was then cultured in a still condition.The medium was renewed regularly and t he cells were subcultured and studied. Results All the c utured cells were urethral diploid cells without fibroblasts.The cells could be subcultured 11～13 generations with a surviving period of 50～60 days. Conclusions The urethral epithelium the cultured cells of young N ew Zealand hare can be cultured in vitro and is able to proliferate within a cer tain period.This provides a strong foundation for the further study on tissue en gineering urethra.

OBJECTIVETo purpose of study aimed at investigating the technique of culturing oral epithelia in vitro and to set up an experimental model for further reconstructing oral mucosa in vitro.

METHODSThe oral mucosa was taken from young New Zealand rabbits, and the mucosa was digested with enzyme and suspended in liquid to form cellular suspension. Being seeded, the cells were cultured motionlessly. The medium was changed regularly and the cells were subcultured.

RESULTSThe cultured cells were all epithelial cells without fibroblasts, and they were proved to be diploid cells. The cells were subcultured in 1-13 generations which survived for 50-60 days.

CONCLUSIONThe oral epithelium of young New Zealand rabbit can be cultured in vitro, maintaining the ability to proliferate in a certain period. It is a pilot study to reconstruct oral tissue in vitro.

Animals ; Animals, Newborn ; Cells, Cultured ; Coculture Techniques ; Epithelial Cells ; cytology ; Female ; L Cells (Cell Line) ; Male ; Mice ; Mouth Mucosa ; cytology ; Rabbits ; Tissue Engineering

To construct the talent cultivating model of psychiatric major suited to our county's actual situation.Jining medical university has conducted explorations and practices persistently in aspects of cultivating objective,enrollment model,course system,teaching quality monitoring system and inspirational education through more than twenty years maneuver.Jining medical university has initially grasped the basic rules of cultivating talents of psychiatric major and has constructed talent cultivating model of psychiatric majors suited to our country's actual situation.

Objective To estimate curative effect of reconstruction of rabbit knee joint cartilage defect with the homogeneitic tissue engineered cartilages.Methods The chondrocytes were isolated and collected from articular cartilages of eight New Zealand white rabbits.The tissue engineered cartilages after culturing chondrocytes and atelocollogen for two days.Cartilage defects were created in both keen joint of twenty-six rab- bits.Complexes of chodrocytes and atelocollagen was grafted into the defect of left knee joint at once as experi- mental group,and no implantation were served as control.General and histological examination were respec- tively performed in both group at four weeks and eight weeks after surgery.Results After implantation,the defects were filled with cartilaginous tissue in experiment group,while there were only tissue in control group. Histologically,defective areas were filled with chondrocytes in experiment group,but only fibroblast in control group.Conclusion The implantation of the tissue engineered cartilages contenting with chondrocytes and atelocollogen can effectively improve reconstruction of rabbit knee joint.

OBJECTIVEThe purpose of this study was to provide the guidelines with respect to the location of the facial vessels, observe the potential reversed flow of the facial artery, and reemphasize the value of color Doppler ultrasound studies in flap planning.

METHODSA study was carried out to investigate the location and dynamics of the facial artery and vein using color Doppler ultrasonography in 12 adults.

RESULTSThe facial artery and the vein were located together at the lower border of the mandible. Around the oral commissure and under the nasal ala, they run apart from each other at variable distances. The reverse flow was observed in the 12 patients after the blood flow of the facial artery was blocked by applying pressure manually at the lower border of the mandible.

CONCLUSIONSThe divergence of the facial vein from the artery is important information in planning of axial pattern flaps. Observation of the reversed flow confirms the possibility of safe elevation of a retrograde flow-arterialized flap based on the distal portion of the facial artery.

Adolescent ; Adult ; Arteries ; diagnostic imaging ; Face ; blood supply ; Female ; Humans ; Male ; Middle Aged ; Ultrasonography, Doppler, Color ; methods ; Veins ; diagnostic imaging

Objective To investigate the long-term effect of free flap in repairing hemifacial atrophy.Methods From April,1991 to April,2015,19 cases (5 males and 14 females) aged between 18 and 51 years (mean,26 years) with hemifacial atrophy were treated by free flap.Hemifacial atrophy affected the left side in 8 patients,and the right side in the remaining 11.Fifteen cases only had a hemifacial soft tissue deficit,the other 4 cases were associated with ipsilateral zygoma dysplasia,in which 1 had ipsilateral scar contracture.The latissimus dorsi muscle flap were applied in 12 cases (2 of which anastomose thoracodorsal nerve with the cervical branch of the facial nerve),the scapular flap in 2 cases,the tensor fascia latae dermal flap with dermal fat flap in 3 cases,and with iliac groin composite tissue flap and ilium groin dermal composite tissue flap in 1 case respectively.Among them,14 flaps had their blood vessels anastomosed to the superficial temporal artery and vein,and 5 to the facial artery and vein.All operations were successfuly accomplished and followed-up for 2-21 years.Results All patients had ideal clinical outcomes with significant improvement in facial symmetry and skin color,and no dysfunction happened.Conclusion The long-term effect of surgical management with free flap in repairing hemifacial atrophy is satisfactory.It is an effective method of clinical treatment.