1.Study on the Stability of the Compatibility of Levofloxacin with Inosine in Sodium Chloride Injection
China Pharmacy 2001;0(10):-
OBJECTIVE:To study the stability of the compatibility of Levofloxacin with inosine in sodium chloride injec?tion.METHODS:The contents of Levofloxacin and inosine were detected by UV-spectrophotometry and the external apper?ance and pH value were observed after Levofloxacin and inosine in NS were mixed within6h at room temperature(20℃).RE_ SULTS:The contents,external apperance and pH value of the mixed solution showed no significant changes within6h.CONCLUSION:Levofloxacin mixed with inosine in NS could keep stable within6h.
2.Study on Compatible Stability of Levofloxacin Hydrochloride Injection with Inosine in Sodium Chloride Injection
China Pharmacy 2005;0(22):-
OBJECTIVE:To study the compatible stability of Levofloxacin hydrochloride injection with Inosine in sodium chloride injection. METHODS:Levofloxacin hydrochloride injection was mixed with Inosine in sodium chloride. Then UV spectrophotometry was applied to detect the change of content at 20 ℃ within 6 hours. The change of appearance,pH value,content of mixture,insoluble particle were also observed. RESULTS:The change of appearance,pH value,content of mixture,insoluble particle in mixture was not found out. CONCLUSION:Levofloxacin hydrochloride injection mixing with Inosine in sodium chloride injection is suitable for clinical use within 6 h.
3.Health education of schistosomiasis control among students in mountainous valley area of Yunnan Province
Yunliang QI ; Hongfeng YANG ; Junhua MA
Chinese Journal of Schistosomiasis Control 2010;22(1):68,86-
Health education of schistosomiasis control among students is an important link of schistosomiasis control in mountainous valley areas of Yunnan Province,which directly affects the control effect in local environment.
4.A case report of laryngeal atypical carcinoid with multiple metastasis.
Hongfeng MA ; Yong QIN ; Wanmin CHENG
Journal of Clinical Otorhinolaryngology Head and Neck Surgery 2014;28(8):575-576
The case of a 49-years-old man complained of pharyngalgia for one year and shortness of breath after activities for one week. Endoscopic laryngeal examination and computed tomography revealed a supraglottic mass. Direct laryngoscopy was performed and biopsy of the mass was carried out. Results of the histopathologic examination and immunohistochemical analysis were consistent with atypical carcinoid tumor of the larynx.
Carcinoid Tumor
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pathology
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Humans
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Laryngeal Neoplasms
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pathology
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Male
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Middle Aged
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Neoplasm Metastasis
5.Posterior short-segment instrumentation without fusion for severe thoracolumbar burst fractures
Xiaolin ZHANG ; Xinlong MA ; Changbao CHEN ; Baoshan XU ; Gongyi Lü ; Xue WANG ; Hongfeng JIANG
Chinese Journal of Trauma 2013;(6):493-497
Objective To evaluate the clinical efficacy of treatment of severe thoracolumbar burst fractures by posterior short-segment instrumentation without spinal fusion and assess radiographic imaging and function recovery after surgery.Methods Thirty-eight patients with severe monosegmental thoracolumbar burst fractures treated between July 2011 and March 2013 were analyzed retrospectively.Operation procedures were posterior short-segment pedicle screw distraction reduction and fixation combined with screw insertion to the injured vertebrae and calcium sulphate augmentation.In addition,there was no need for posterolateral interbody fusion.X-ray and CT were performed before and after operation to evaluate local kyphotic angle,anterior fractured vertebral body height and canal encroachment.Visual analogue scale (VAS) and Oswestry disability index (ODI) were assessed before and after operation as well as in follow-up.Results All patients were followed up for average 14 months (range,3-20 months).Local kyphotic angle was (21.2 ±4.3)° before operation,(3.5 ± 1.8)°immediately after operation,and (4.8 ± 2.7) ° in final follow-up.Relative anterior vertebral height was (54.8 ± 14.6)% before operation,(91.7 ± 8.0)% after operation,and (87.2 ± 6.0)% in final follow-up.Mean canal encroachment was (48.0 ± 4.5)% preoperatively,(23.8 ± 7.8)%postoperatively,and (8.8 ± 4.6) % in final follow-up.In final follow-up,six patients with American Spinal Injury Association (ASIA) grade C on admission showed improvement to grade D (n =2) and grade E (n =4) ; 10 patients with ASIA grade E on admission showed improvement to grade E; 22 patients with grade E had no changes.ODI and VAS scored 15.5 ±8.8 and 2.3 ±0.8 in final follow-up with substantial improvement from those before operation (P < 0.01).Complications from internal fixation were not found during follow-up.Conclusion Posterior short-segment fixation without fusion is one of the foremost effective methods for severe thoracolumbar burst fractures,for it can effectively restore the sagittal spinal alignment and the fractured vertebral body height.
6.The immunological effect of anti-leukemic tumor induced by eosinophilic granulocyte
Lifei SUN ; Qiangqiang WU ; Hongfeng HAO ; Xiangshan MA ; Yanhui DU ; Guichen WANG ; Jinbiao ZHANG
Chinese Journal of Internal Medicine 2013;(5):395-399
Objective To investigate the biological effect of anti-leukemic cells induced by eosinophilic granulocyte (EOS) in bone marrow of patients with chronic myelogenous leukemia (CML).Methods The BCR-ABL fusion gene as well as the expression of IL-12 and IL-17 mRNA were performed by RT-PCR.The serum concentrations of cytokine IL-12 and IL-17 were determined by enzyme-linked immuno sorbent assay (ELISA).Immunochemistry staining and cytochemistry staining were used to observe the peroxydase (POX) and human Leukocyte antigen (HLA)-DR expression of EOS in bone marrow.Immunofluorescence staining was used to observe mannose receptor (MR),IL-12,IL-17A and IL-17receptor A (IL-17RA) expression of EOS.The results between the CML patients and the healthy controls were compared.Results Serum levels of IL-12 and IL-17 were higher in the 60 CML patients [(196.33 ±21.79) ng/L and (36.55-±3.01) ng/L] than those in the controls [(96.60 ±4.92) ng/L and (23.74 ±1.36) ng/L].In the 32 patients with activated EOS,the levels of IL-12 and IL-17 were (273.12 ± 17.16)ng/L and (40.11 ± 6.13) ng/L,which were significantly higher than those in the non-activated EOS [(126.16 ± 14.27) ng/L and (28.14 ±5.29) ng/L] (P values <0.01).IL-12 and IL-17 mRNA were expressed in activated EOS,while BCR-ABL fusion gene was not found.The amounts of EOS were increased abnormally in the bone marrow and peripheral blood of the CML patients with POX positive staining in the cytoplasm and weakly positive HLA-DR staining.It was observed easily by a microscope that EOS could attack leukemic cells in bone marrow through adhesion,capture and phagocytosis.Activated EOS could express IL-12,IL-17A and MR,which was related with the serum levels of these cytokines.Conclusions Activated EOS in bone marrow of CML patients could express IL-12 and IL-17.Activated EOS could induce coup injury to leukemic cell by releasing POX and expressing IL-12 and IL-17.It can also capture or swallow target cells via the expression of MR on the membrane.EOS may play an important role in the anti-tumor immunologic function in bone marrow of CML patients.
7.Intervention on Apoptosis of Renal Tubular Epithelial Cell in Chronic Lead Poisoning Rats by Quqian Granules
Jin ZHOU ; Sha XUE ; Wei MA ; Geng ZHANG ; Ying ZHANG ; Hongfeng XU ; Lu CHENG
China Pharmacist 2014;(5):719-722
Objective:To investigate the protective effect of Yiqi Huazhi recipe Quqian granules on rat renal tubular cell apoptosis induced by lead poisoning. Methods:Totally 60 Wistar rats were divided into 2 groups, 12 in the control group and the others in the model group. Chronic lead poisoning model was made by drinking 0. 02% lead acetate water for 60 days. Then the lead poisoning rats were randomly divided into four groups, high-dose Quqian granules group (3. 0 g·kg-1·d-1), low-dose Quqian granules group (0. 6 g·kg-1 ·d-1 ) , positive control group ( calcium disodium edentate plus procaine, im, 50 mg·kg-1 ·d-1 ) and model group. Seven treatment courses were carried out in the first three groups with every 4-d as one course and 4-d withdrawal period between every two courses. After 60 days, the change of lead in blood and kidney was observed by atomic absorption spectrometry,the apoptosis of kidney tissues was studied by TUNEL, the expression of Bcl-2 protein was detected by immunohistochemical methods and the expression of p53 was studied by Western Blotting. Results:Compared with the control group, the body weight, hemoglobin and the expression of Bcl-2 in the model group were decreased significantly(P<0.01)those in, and Pb in blood(0.990 ±0.443)μg·ml-1, Pb in kidney(51.33 ± 5. 16)μg·ml-1 , the apoptosis of tubular epithelial cell(4. 148 ± 0. 414) and the expression of p53 protein (1. 868 ± 0. 139) were significantly higher (P<0. 05). Compared with the those in model group, the body weight, hemoglobin and Bcl-2 in high-dose group were increased significantly(P<0.01), and the blood lead level (0.082 ±0.015)μg·ml-1, the kidney lead level (6.38 ±0.97)μg ·ml-1 , the apoptosis of tubular epithelial cell(1. 412 ± 0. 109) and p53 protein expression(1. 164 ± 0. 172) were significantly lower (P<0. 05). Conclusion:Lead may induce high expression of p53,low expression of Bcl-2 and promote the apoptosis of renal tubular epithelial cells. It is proven that Yiqi Huazhi recipe Quqian granules can inhibit the expression increase of p53 and the expression de-crease of Bcl-2 resulting in the reduction of the renal tubular apoptosis to allivate the renal injury caused by lead.
8.A comparative study of the effects of off-pump versus on-pump CABG operations on renal function in elderly patients.
Huaibin WANG ; Wenjun ZHEN ; Hongfeng TONG ; Xiaokang OUYANG ; Yaoguang SUN ; Yujian MA
Chinese Journal of Geriatrics 2000;0(06):-
0. 05). Creatinine clearance improved significantly in group C from a mean value of (90?23)ml/min preoperatively to (139?46)ml/min during operation (P
9.Mid- and long-term follow-up for the graft patency after coronary artery bypass graft surgery
Wei CHENG ; Wenjun ZHEN ; Yujian MA ; Xiaokang OUYANG ; Hongfeng TONG ; Yaoguang SUN ; Yongzhong WANG ; Wen HUANG
Chinese Journal of Thoracic and Cardiovascular Surgery 2010;26(5):325-328
Objective To analyze the mid- and long-term results after coronary artery bypass graft surgery (CABG), to evaluate the accuracy of multi-detector CT coronary angiography (MDCT) on the graft patency status and to compare the patency for different grafts. Methods One hundred and one cases underwent CABG from June 1992 to March 2008 were followed up by searching the database of MDCT (42 cases) and selective coronary angiography (SCA, 59 cases). The mean following up period was (66.79 ±44.27) months. Three hundreds and 10 grafts including 115 arterial and 195 venous were analyzed.A comparison of the patency rate between the arterial and venous grafts was also carried out. At the mean time, 2 groups were divided according to the paft lesions( patency of not). Uunivariate analysis and Logistic regression analysis were made for statistics. Results At the follow-up of (53.93 ± 36.80) months, the patent rate of LIMA, RA, SV and CV examined by MDCT was 94.7%, 92.0%, 85.9% and 60.0%, respectively. The patent rate for MDCT patients with angina was 83.5%, and 95.2% for angina-free group ( P = 0.046). At the follow-up of (75.95 ±47.09) months, the patent rate of LIMA, RA, SV and CV examined by SCA was 87.1%, 81.0%, 53.6% and 57. 1%, respectively. In SCA patients the patent rate was 62.0% for angina group and 100% for angina-free group ( P = 0.025 ). According to logistic regression analysis, only the postoperative period was statistically related to the graft lesion. Conclusion MDCT could find out the occlusions and obvious stenoses of the grafts after CABG. It might be served as a noninvasive method to evaluate the grafts patency postoperatively. The mid- and long-term patency of arterial grafts is better than venous grafts. Graft lesion is an important factor leading to postoperative recurrent angina pectoris. The severity of the graft lesion has a close relation to the lenth of postoperative period.
10.Application of mini-open approach beside costodiaphragmatic recess in anterior thoracolumbar spine surgery
Baoshan XU ; Xinlong MA ; Qun XIA ; Xiaolin ZHANG ; Hongfeng JIANG ; Qiang YANG ; Yue LIU ; Ning JI
Tianjin Medical Journal 2015;(2):196-198,199
Objective To analyze the value of mini-open approach beside costodiaphragmatic recess in thoracolumbar spine surgery. Methods This approach was applied in 31 anterior thoracolumbar spine surgeries, including 22 men and 9 women, with a mean age of 41 years old (range, 26-58 yrs). The diagnosis were burst fractures in 27 cases (T12 level in 12 cas?es and L1 level in 15 cases) and disc herniations with osteochondrosis in 4 cases. An antero-lateral 10-15 (average is 12) cm incision was performed, then the 11th rib was resected and the extraperitoneal space below diaphragma was disconnected. The pleura fold was identified beneath the rib bed, so the gap beside the costdiaphragmatic recess was entered through an in?cision beyond the fold. The diaphragm and medial arcuate ligament were clipped and vertebral body from T11 to L2 were ex?posed. Results The lateral side of T11 to L2 vertebral body was sufficiently exposed in all the 31 patients. In 26 patients, the pleura fold was beyond the bed of the 11th rib, so the 11th intercostals vessel and nerve were exposed and protected, and the costodiaphragmatic recess was reached through the superior border of the 12th rib. Laceration of pleura occurred in 4 cases af?ter it was sutured, but the extra-pleura approach could still be used after repairing without invading into thorax. Fixation and fusion were performed from T11 to L2. Complications include intercostals nerve pain were seen in 3 cases, which resolved with conservative treatment. Conclusion The mini-open approach beside costodiaphragmatic recess can be used in anterior thoraclumbar spine surgery with sufficient explosion and minimum injury in which thoracic cavity.