Objective To identify the chemoresistant factors predicting the response to preoperative chemotherapy and clinicopathological prognosis in bulky cervical cancer. Methods 68 patients with bulky cervical carcinoma treated with two courses of intraarterial infusion of cisplatin 80mg, 5 fluorouracil(5Fu) 1500mg and AT 1258 or EADR 60mg, followed by radical hysterectomy and pelvic lymphnodenectomy at our hospital between 1996-1999 were retrospectively reviewed. Expressions of the chemoresistance related proteins, such as P glycoprotein glutathione S transferase ?(GST ?), and proliferating cell nuclear antigen(PCNA) in the tumor cells were examined by immunohistochemistry in previous biopsy specimens. These results were compared with the chemotherapeutic response obtained by gynecological examination and vagina ultrasonic. 68 patients were followed up. SPSS 8.0 was used. Results P glycoprotein expression rate was 31% and GST ? expressioin rate was 51%. There were 38 patients whose PCNA labellings were more than 50% and 30 less than 50%. The total chemotherapeutic response rate was 84%. Chemotherapeutic response rate was significantly correlated with P glycoprotein expression( P =0.013) and PCNA labelling ( P =0.001), but not GST ? expression in the tumor cells. Parautrial involvement and lymph node metastasis were independent factors for prognosis in this group. The survival rate in MDR(+) group was lower than MDR(-) group. No significant correlation between eigher the expression of GST ? or PCNA. Conclusions The expression of P glycoprotein and PCNA is potentially useful for predicting the response to preoperative chemotherapy for cervical cancer. The parautrial involvement and lymph node metastasis were independent prognostic factors for the survival rate including. the expression of P glycoprotein.

Objective To evaluate the significance of expression of hMLH1 and hMSH2 in detecting hereditary nonpolyposis colorectal carcinoma (HNPCC). Methods Colorectal carcinomas from 66 patients were examined by immunohistochemistry for the expressions of hMLH1 and hMSH2 using commercially available monoclonal antibodies. Immunohistochemical patterns of tumors from 19 HNPCC patients (Group A), 20 suspected HNPCC patients (Group B), 14 patients whose clinical features conformed to the Bethesda guideline (Group C), and 13 sporadic colorectal cancer patients (Group D) were compared. Results The absence or low expression of hMLH1 and hMSH2 was revealed in 72.8% patients of group A, 60.0% in group B, 28.4% in group C and 7.7% in group D. The absence or low protein expression of hMLH1 and hMSH2 was significantly correlated with HNPCC (P=0.0008). The absence or low expression of hMLH1 was higher detection rate than that hMSH2 (P=0.01). Conclusions HNPCC patients can be identified by immunohistochemical methods according to the expression of hMLH1 and hMSH2, which may be used in clinical practice and research. Immunohistochemical analysis of hMLH1 and hMSH2 may predict the presence of corresponding gene mutations. Gene mutation in hMLH1 might be higher than that in hMSH2 in Chinese HNPCC.

Purpose:To explore the clinical and pathological characters and immunophenotype of 2 cases nasal type extranodal NK/T cell lymphoma primarily derived from adrenal gland.Methods:The clinical and pathological features of the 2 cases were analyzed and the immunostaining for UCHL-1,CD43,CD3,CD56,TIA-1 and LMP1 was performed using EnVision TM . IgH and TCR? gene rearrangement was analyzed by using PCR techniques.Results:Both patients were male and aged 50 and 51. Occupying masses in their right adrenal glands was detected. The two tumors showed similar morphological features, composing of small to middle sized and multi formed cells and growing invasive. Inflammatory background,necrosis and vessel involvement were seen. Positive surface staining of UCHL-1,CD43,CD56 and cytoplasma staining of CD3 was demonstrated. TIA-1 and LMP1 expression was also detected. No clonal rearrangement in both IgH and TCR? genes was detected.Conclusions:Nasal type extranodal NK/T cell lymphoma can occur primarily in adrenal gland. It should be distinguished from other small cell malignant tumor of this site.

Purpose: To study the immunophenotype and the expression of cytotoxic granule proteins of nasal NK/T cell lymphoma and its significance. Methods: 44 cases of nasal NK/T cell lymphoma were studied by the two-step method of DAKO EnVisionTM using a series of antibodies including CD3, CD20, CD43, CD45, CEI45RO, CD56, CD57, CD79?, TIA-1, granzyme-B and perform. Results: All the cases of nasal NK/T cell lymphoma were CD45 positive. 43% of the cases expressed CD3 with positive signal located in the cytoplasm, which was different from peripheral T cell lymphoma. 45% and 52% of the cases were CD43 and CD45RO positive respectively. Cases that reacted to CD56 accounted for 52% of the cases, 43% of which were also positive to CD3. Concerning the reactions to both CD3 and CD56, 10 cases showed CD3 + CD56 + , 13 showed CD3-CD56 + , 9 were CD3 + CD56- and 12 were CD3-CD56-. None of the 44 cases showed positive reaction to CD20, CD79? and CD57. All cases were reactive to TIA-1. 93% and 95% of the cases showed the reactions to granzyme-B and perform. All the controls were negative to TIA-1, granzyme-B and perform. Conclusions: The immunophenotypes of nasal NK/T cell lymphoma showed less consistency. CD56 was not always positive in the cases of this tumor. The different locations of the positive signal to CD3 showed that the cell lineage of this tumor was different from T lymphocytes. The high frequency of the staining by cytotoxic granule proteins, TIA-1, granzyme-B and perform, showed that these cells may have originated from NK cells. The distinctive differences in immunohistochemical staining of cytotoxic granule proteins in nasal NK/T cell lymphoma make their detection very useful and important in diagnosis and differential diagnosis.

Background and purpose:Blood vessel metastasis often occurs after the operation of rectal cancer of stage Ⅱ and Ⅲ,angiogenesis is an important step of the procedure.Cycloxygenase-2(COX-2) and vascular endothelial growth factor(VEGF-C) are correlated to angiogenesis. The purpose of this study is to investigate the expressions of Cycloxygenase 2(COX-2) and vascular endothelial growth factor(VEGF-C) in rectal cancer of stage Ⅱ and Ⅲ,to explore the relationships between them and the tumor biological characteristics,tumor angiogenesis.Methods:The expressions of COX-2,VEGF-C and the microvessel density(MVD) were detected by immunohistochemical staining.Results:①The positive rates of COX-2 and VEGF-C expression in rectal cancer were 72.5%,higher than those in peritumoral normal tissue(P

Purpose:To evaluate the relationship between the expressions of MDR and GST-? and the response to neoadjuvant chemotherapy and prognosis in cervical carcinoma. Methods:The expressions of MDR and GST-? were examined by Envision immunohistochemistry using pretreatment biopsy specimens. The stage distribution of 57 patients in the study was 7 stage Ib, 35 stage IIa, 15 stage IIb. Treatment consisted of 2 courses of neoadjuvant chemotherapy followed by radical hysterectomy and lymphonectomy. If the patient was found to have parametrial involvement, pelvic lymph node metastasis, microscopic tumor emboli or disease in vagina stemp, she was given adjuvant radiotherapy after operation. All patients were followed up and the median follow-up time was 35 months (21-66 months). Statistical method used was SPSS 8.0 package. Results:There were 14 patients with cervical carcinoma who had expression of MDR. The rate of expression of MDR was 24.6% (14/57). Also there were 29 patients who had expression of GST-?. The rate of expression was 50.8% (29/57). The total response rate of neoadjuvant chemotherapy was 81%. The complete clinical response rate was 19% (11/57) and partial response rate was 61% (35/57). All patients were treated by operation following chemotherapy and 13 patients were given adjuvant radiotherapy after operation. The 5-year survival rate in stage Ib was 100%, stage IIa 90% and stage IIb 78.5%. The results showed the expression of MDR was related to response of neoadjuvant chemotherapy and 5-year survival. It is 93%(40/43) in group of MDR(-) and 43%(6/14) in group of MDR(+)( P =0.001). But it was not related to FIGO, histopathologic, parametrial involvement, and pelvic lymph nodes metastasis. The expression with GST-? only related to response of chemotherapy. The response rate in the group with expression of GST-? is 69% and it is 93% in the group with GST-?(-)( P =0.02).Conclusions:The response rate of neoadjuvant chemotherapy in group of MDR(-) and GST-?(-) was better than in the group with expression of MDR and GST-?. The measure of MDR and GST-? is helpful to predict the response of neoadjuvant chemotherapy in cervical cancer and MDR may related to prognosis of cervical cancer.

0.05).In the followed-up patients,univariant analysis demonstrated that the expressions of CD44 and Ezrin and their coexpression were correlated with disease free survival(DFS)rate(P

Purpose:To report a seldom case of benign glandular schwannoma and review its pathological characteristic. Methods:The tumor was observed by histology, histochemistry and immunohistochemistry. Results:Histologically, the tumor is composed of spindle cell component showing typical schwannoma and collections of glandular component with the expressions of Leu 7,GFAP,MBP,CEA,AE1/AE3 and 34?E12. Conclusions:The histogenesis of the glandular schwannoma remains uncertain. In our opinion, the glands within the tumor, when examined immunohistochemically, probably represent entrapped dermal appendageal glands with proliferation. [

Purpose:To study the histogenesis,the differential diagnosis and the prognosis of melanotic schwannoma.Methods:The age of the patients was 4,17,28 years old, respectively.Three cases of melanotic schwannoma were examined using histologic (HE), immunohistochemical (ABC method) and electron microscopic techniques. Results: The diameter of tumor was 2,4,8, respectively. Histological examination showed pigmented spindle cells arranged in interlacing fascicles and occasional nuclear palisading. The pigmented polygonal cells were loosely arranged in sheets. After bleaching, the nuclei were oval and some showed slight pleomoprohism with prominent nucleoli, and nuclear mitoses were found. There was local invasion in two cases. Immunohistochemical stains for S 100 protein, HMB45, Vim, Leu 7, NSE were positive. Ultrastructrual examinations showed a lot of melanosomes in all stages of development and seldom basal lamina. Conclusions:①Melanotic schwannoma usually occurs in young adults. Most of the tumors are benign or only potentially malignant. Malignant MS is rare,and require follow up. ②MS originate from neural crest with a proliferation of the bipotential cell.

Purpose:To evaluate the efficacy and toxicity of imatinib mesylate (STI571) in the treatment of unresectable and/or metastatic gastrointestinal stromal tumors (GIST).Methods:Ten patients with unresectable and/or metastatic GIST received imatinib at doses of 400 mg qd.Results:Among 9 evaluable patients, 4 achieved a partial response and 4 had stable disease. Ten patients were evaluable for toxicities. The nonhaematological toxicities included edema (mainly periorbital edema), abdominal pain, diarrhoea, nausea/vomiting, fatigue, intratumoural bleeding, intermittent muscle cramps and conjunctivitis. Myelosuppression was an infrequent side effect.Conclusions:Imatinib mesylate is an inhibitor of tyrosine kinase and has been proven to be active in patients with unresectable or metastatic GIST. Toxicity is acceptable.